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PFKL (NM_002626) Human Tagged ORF Clone
CAT＃: RC200922
PFKL (Myc-DDK-tagged)-Human phosphofructokinase, liver (PFKL), transcript variant 2
ORF Plasmid: tGFP
"NM_002626" in other vectors (6)
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CNY 8,568.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP-PFK; PFK-B; PFK-L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200922 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCGCGGGCAAGGCCATCGGCGTCCTGACCAGCG GCGGCGACGCGCAAGGCATGAACGCTGCTGTCCGGGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAA AGTCTTCCTCATCTACGAGGGCTATGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGG CTGAGCGTCTCCAACATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCA CCAGGGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGTGCGTCATCGG CGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCAGCCTGCTGGAGGAGCTGGTG GCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACTCGCACCTGAACATCGCGGGCCTAGTGGGCT CCATCGATAACGACTTCTGCGGCACCGACATGACCATCGGCACGGACTCGGCCCTCCACCGCATCATGGA GGTCATCGATGCCATCACCACCACTGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGG CACTGCGGGTACCTGGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTC CACCCGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTGGGTCCCGACT GAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCATCTCGTCCAGCTACGTGAAG GACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAACTGTGCTGGGCCACGTGCAGCGGGGAGGGA CGCCCTCTGCCTTCGACCGGATCCTGAGCAGCAAGATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGC CACGCCTGACACGCCGGCCTGCGTGGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAG TGCGTGCAGATGACCAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTCC GTGGTGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCCCCAAGGAGAA GTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCATGAATGCGGCCGTGCGCTCG GCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACGTGGTGCACGATGGCTTCGAAGGCCTAGCCA AGGGTCAGGTGCAAGAAGTAGGCTGGCACGACGTGGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGG GACCAAGAGGACCCTGCCCAAGGGCCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCAC GCCCTGCTGGTGGTCGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGCT ACGAGGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTGGCACCGACTT CAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACCGCATCAAACAGTCTGCCTCG GGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGGGTTACTGTGGCTACCTGGCCACCGTGACTG GCATTGCTGTGGGGGCCGACGCCGCCTACGTCTTCGAGGACCCTTTCAACATCCACGACTTAAAGGTCAA CGTGGAGCACATGACGGAGAAGATGAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGC CATGACTACTACACCACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAACGGCGTCTTCGACTGCA GGACCAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACTATGGGACCAA GCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTTACCGCAAGGGACGGGTGTTC GCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGAAGAAGGCGGCGGCCTTCAGCCCCGTCACTG AGCTCAAGAAAGACACTGATTTCGAGCACCGCATGCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCAT GCTGAAGATGCTGGCACAATACCGCATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACC CGCCGCACCCTGAGCATGGACAAGGGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200922 protein sequence
Red=Cloning site Green=Tags(s) MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANW LSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNLCVIGGDGSLTGANIFRSEWGSLLEELV AEGKISETTARTYSHLNIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGR HCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVK DLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLME CVQMTKEVQKAMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRS AVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESIVENIRIYGIH ALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSAS GTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKVNVEHMTEKMKTDIQRGLVLRNEKC HDYYTTEFLYNLYSSEGNGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVF ANAPDSACVIGLKKKAAAFSPVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVT RRTLSMDKGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002626 |
| ORF Size | 2340 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002626.6 |
| RefSeq Size | 2934 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 5211 |
| UniProt ID | P17858 |
| Domains | PFK |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway |
| MW | 85 kDa |
| Gene Summary | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200922L1 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 2, Myc-DDK-tagged |
CNY 10,968.00 |
|
| RC200922L2 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200922L3 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200922L4 | Lenti ORF clone of Human phosphofructokinase, liver (PFKL), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200922 | PFKL (tGFP-tagged) - Human phosphofructokinase, liver (PFKL), transcript variant 2 |
CNY 4,370.00 |
|
| SC319353 | PFKL (untagged)-Human phosphofructokinase, liver (PFKL), transcript variant 2 |
CNY 8,576.00 |
