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RhoGDI (ARHGDIA) (NM_004309) Human Tagged ORF Clone
CAT＃: RC200902
ARHGDIA (Myc-DDK-tagged)-Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2
ORF Plasmid: tGFP
"NM_004309" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200902 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGCAGGAGCCCACAGCCGAGCAGCTGGCCCAGATTGCAGCGGAGAACGAGGAGGATGAGCACT CGGTCAACTACAAGCCCCCGGCCCAGAAGAGCATCCAGGAGATCCAGGAGCTGGACAAGGACGACGAGAG CCTGCGAAAGTACAAGGAGGCCCTGCTGGGCCGCGTGGCCGTTTCCGCAGACCCCAACGTCCCCAACGTC GTGGTGACTGGCCTGACCCTGGTGTGCAGCTCGGCCCCGGGCCCCCTGGAGCTGGACCTGACGGGCGACC TGGAGAGCTTCAAGAAGCAGTCGTTTGTGCTGAAGGAGGGTGTGGAGTACCGGATAAAAATCTCTTTCCG GGTTAACCGAGAGATAGTGTCCGGCATGAAGTACATCCAGCATACGTACAGGAAAGGCGTCAAGATTGAC AAGACTGACTACATGGTAGGCAGCTATGGGCCCCGGGCCGAGGAGTACGAGTTCCTGACCCCCGTGGAGG AGGCACCCAAGGGTATGCTGGCCCGGGGCAGCTACAGCATCAAGTCCCGCTTCACAGACGACGACAAGAC CGACCACCTGTCCTGGGAGTGGAATCTCACCATCAAGAAGGACTGGAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200902 protein sequence
Red=Cloning site Green=Tags(s) MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNV VVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKID KTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004309 |
| ORF Size | 612 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004309.6 |
| RefSeq Size | 1920 bp |
| RefSeq ORF | 615 bp |
| Locus ID | 396 |
| UniProt ID | P52565 |
| Domains | Rho_GDI |
| Protein Families | Druggable Genome |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 23.2 kDa |
| Gene Summary | This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
EphrinB1 promotes cancer cell migration and invasion through the interaction with RhoGDI1
,Cho, HJ;Hwang, YS;Yoon, J;Lee, M;Lee, HG;Daar, IO;,
Oncogene
,PubMed ID 29059157
[RhoGDI]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200902L1 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200902L2 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200902L3 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200902L4 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200902 | ARHGDIA (tGFP-tagged) - Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2 |
CNY 4,000.00 |
|
| SC117424 | ARHGDIA (untagged)-Human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA), transcript variant 2 |
CNY 2,400.00 |
