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PFKP (NM_002627) Human Tagged ORF Clone
CAT＃: RC200673
PFKP (Myc-DDK-tagged)-Human phosphofructokinase, platelet (PFKP), transcript variant 1
ORF Plasmid: tGFP
"NM_002627" in other vectors (6)
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CNY 5,744.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP-PFK; PFK-C; PFK-P; PFKF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200673 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCGGACGACTCCCGGGCCCCCAAGGGCTCCTTGCGGAAGTTCCTGGAGCACCTCTCCGGGGCCG GCAAGGCCATCGGCGTGCTGACCAGCGGCGGGGATGCTCAAGGTATGAACGCTGCCGTCCGTGCCGTGGT GCGCATGGGTATCTACGTGGGGGCCAAGGTGTACTTCATCTACGAGGGCTACCAGGGCATGGTGGACGGA GGCTCAAACATCGCAGAGGCCGACTGGGAGAGTGTCTCCAGCATCCTGCAAGTGGGCGGGACGATCATTG GCAGTGCGCGGTGCCAGGCCTTCCGCACGCGGGAAGGCCGCCTGAAGGCTGCTTGCAACCTGCTGCAGCG CGGCATCACCAACCTGTGTGTGATCGGCGGGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAG TGGAGTGGGCTGCTGGAGGAGCTGGCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAGAAGTACGCCT ACCTCAACGTGGTGGGCATGGTGGGCTCCATCGACAATGATTTCTGCGGCACCGACATGACCATCGGCAC GGACTCCGCCCTGCACAGGATCATCGAGGTCGTCGACGCCATCATGACCACGGCCCAGAGCCACCAGAGG ACCTTCGTTCTGGAGGTGATGGGACGACACTGTGGGTACCTGGCCCTGGTGAGTGCCTTGGCCTGCGGTG CGGACTGGGTGTTCCTTCCAGAATCTCCACCAGAGGAAGGCTGGGAGGAGCAGATGTGTGTCAAACTCTC GGAGAACCGTGCCCGGAAAAAAAGGCTGAATATTATTATTGTGGCTGAAGGAGCAATTGATACCCAAAAT AAACCCATCACCTCTGAGAAAATCAAAGAGCTTGTCGTCACGCAGCTGGGCTATGACACACGTGTGACCA TCCTCGGGCACGTGCAGAGAGGAGGGACCCCTTCGGCATTCGACAGGATCTTGGCCAGCCGCATGGGAGT GGAGGCAGTCATCGCCTTGCTAGAGGCCACCCCGGACACCCCAGCTTGCGTCGTGTCACTGAACGGGAAC CACGCCGTGCGCCTGCCGCTGATGGAGTGCGTGCAGATGACTCAGGATGTGCAGAAGGCGATGGACGAGA GGAGATTTCAAGATGCGGTTCGACTCCGAGGGAGGAGCTTTGCGGGCAACCTGAACACCTACAAGCGACT TGCCATCAAGCTGCCGGATGATCAGATCCCAAAGACCAATTGCAACGTAGCTGTCATCAACGTGGGGGCA CCCGCGGCTGGGATGAACGCGGCCGTACGCTCAGCTGTGCGCGTGGGCATTGCCGACGGCCACAGGATGC TCGCCATCTATGATGGCTTTGACGGCTTCGCCAAGGGCCAGATCAAAGAAATCGGCTGGACAGATGTCGG GGGCTGGACCGGCCAAGGAGGCTCCATTCTTGGGACAAAACGCGTTCTCCCGGGGAAGTACTTGGAAGAG ATCGCCACACAGATGCGCACGCACAGCATCAACGCGCTGCTGATCATCGGTGGATTCGAGGCCTACCTGG GACTCCTGGAGCTGTCAGCCGCCCGGGAGAAGCACGAGGAGTTCTGTGTCCCCATGGTCATGGTTCCCGC TACTGTGTCCAACAATGTGCCGGGTTCCGATTTCAGCATCGGGGCAGACACCGCCCTGAACACTATCACC GACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAACCAAGCGGCGCGTGTTCATCATCGAGACCATGG GCGGCTACTGTGGCTACCTGGCCAACATGGGGGGGCTCGCGGCCGGAGCTGATGCCGCATACATTTTCGA AGAGCCCTTCGACATCAGGGATCTGCAGTCCAACGTGGAGCACCTGACGGAGAAAATGAAGACCACCATC CAGAGAGGCCTTGTGCTCAGAAATGAGAGCTGCAGTGAAAACTACACCACCGACTTCATTTACCAGCTGT ATTCAGAAGAGGGCAAAGGCGTGTTTGACTGCAGGAAGAACGTGCTGGGTCACATGCAGCAGGGTGGGGC ACCCTCTCCATTTGATAGAAACTTTGGAACCAAAATCTCTGCCAGAGCTATGGAGTGGATCACTGCAAAA CTCAAGGAGGCCCGGGGCAGAGGAAAAAAATTTACCACCGATGATTCCATTTGTGTGCTGGGAATAAGCA AAAGAAACGTTATTTTTCAACCTGTGGCAGAGCTGAAGAAGCAAACGGATTTTGAGCACAGGATTCCCAA AGAACAGTGGTGGCTCAAGCTACGGCCCCTCATGAAAATCCTGGCCAAGTACAAGGCCAGCTATGACGTG TCGGACTCAGGCCAGCTGGAACATGTGCAGCCCTGGAGTGTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC200673 protein sequence
Red=Cloning site Green=Tags(s) MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDG GSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKE WSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQR TFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQN KPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGN HAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGA PAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEE IATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTIT DTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTI QRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAK LKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDV SDSGQLEHVQPWSV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002627 |
| ORF Size | 2352 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002627.1 |
| RefSeq Size | 2657 bp |
| RefSeq ORF | 2355 bp |
| Locus ID | 5214 |
| UniProt ID | Q01813 |
| Domains | PFK |
| Protein Families | Druggable Genome |
| Protein Pathways | Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway |
| MW | 85.6 kDa |
| Gene Summary | This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
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Protein/Peptide Tags
,Johnson, M;,
Labome Dec 2013.
[PFKP]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200673L1 | Lenti ORF clone of Human phosphofructokinase, platelet (PFKP), transcript variant 1, Myc-DDK-tagged |
CNY 8,144.00 |
|
| RC200673L2 | Lenti ORF clone of Human phosphofructokinase, platelet (PFKP), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC200673L3 | Lenti ORF clone of Human phosphofructokinase, platelet (PFKP), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC200673L4 | Lenti ORF clone of Human phosphofructokinase, platelet (PFKP), transcript variant 1, mGFP tagged |
CNY 8,144.00 |
|
| RG200673 | PFKP (tGFP-tagged) - Human phosphofructokinase, platelet (PFKP), transcript variant 1 |
CNY 7,344.00 |
|
| SC118507 | PFKP (untagged)-Human phosphofructokinase, platelet (PFKP), transcript variant 1 |
CNY 5,752.00 |
