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NNMT (NM_006169) Human Tagged ORF Clone
CAT＃: RC200641
NNMT (Myc-DDK-tagged)-Human nicotinamide N-methyltransferase (NNMT)
ORF Plasmid: tGFP
"NM_006169" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200641 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCAGGCTTCACCTCCAAGGACACCTATCTAAGCCATTTTAACCCTCGGGATTACCTAGAAAAAT ATTACAAGTTTGGTTCTAGGCACTCTGCAGAAAGCCAGATTCTTAAGCACCTTCTGAAAAATCTTTTCAA GATATTCTGCCTAGACGGTGTGAAGGGAGACCTGCTGATTGACATCGGCTCTGGCCCCACTATCTATCAG CTCCTCTCTGCTTGTGAATCCTTTAAGGAGATCGTCGTCACTGACTACTCAGACCAGAACCTGCAGGAGC TGGAGAAGTGGCTGAAGAAAGAGCCAGAGGCCTTTGACTGGTCCCCAGTGGTGACCTATGTGTGTGATCT TGAAGGGAACAGAGTCAAGGGTCCAGAGAAGGAGGAGAAGTTGAGACAGGCGGTCAAGCAGGTGCTGAAG TGTGATGTGACTCAGAGCCAGCCACTGGGGGCCGTCCCCTTACCCCCGGCTGACTGCGTGCTCAGCACAC TGTGTCTGGATGCCGCCTGCCCAGACCTCCCCACCTACTGCAGGGCGCTCAGGAACCTCGGCAGCCTACT GAAGCCAGGGGGCTTCCTGGTGATCATGGATGCGCTCAAGAGCAGCTACTACATGATTGGTGAGCAGAAG TTCTCCAGCCTCCCCCTGGGCCGGGAGGCAGTAGAGGCTGCTGTGAAAGAGGCTGGCTACACAATCGAAT GGTTTGAGGTGATCTCGCAAAGTTATTCTTCCACCATGGCCAACAACGAAGGACTTTTCTCCCTGGTGGC GAGGAAGCTGAGCAGACCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200641 protein sequence
Red=Cloning site Green=Tags(s) MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQ LLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLK CDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQK FSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006169 |
| ORF Size | 792 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006169.3 |
| RefSeq Size | 1579 bp |
| RefSeq ORF | 795 bp |
| Locus ID | 4837 |
| UniProt ID | P40261 |
| Domains | NNMT_PNMT_TEMT |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism |
| MW | 29.6 kDa |
| Gene Summary | N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A Proteogenomic Atlas of Clear Cell Renal Cell Carcinoma in a Chinese Population
,Ding, C;Qu, Y;Feng, J;Wu, X;Bai, L;Xu, W;Zhu, L;Liu, Y;Xu, F;Zhang, X;Yang, G;Lv, J;Chen, X;Shi, G;Wang, H;Cao, D;Xiang, H;Li, L;Tan, S;Gan, H;Sun, M;Zhang, H;Zhao, J;Ye, D;,
Research Square
[NNMT]
|
|
BRCA1 Deficiency Upregulates NNMT Which Reprograms Metabolism and Sensitizes Ovarian Cancer Cells to Mitochondrial Metabolic Targeting Agents
,Kanakkanthara, A;Kurmi, K;Ekstrom, TL;Hou, X;Purfeerst, ER;Heinzen, EP;Correia, C;Huntoon, CJ;O'Brien, D;Wahner Hendrickson, AE;Dowdy, SC;Li, H;Oberg, AL;Hitosugi, T;Kaufmann, SH;Weroha, SJ;Karnitz, LM;,
Cancer Res.
,PubMed ID 31619387
[NNMT]
|
|
NNMT silencing activates tumor suppressor PP2A, inactivates oncogenic STKs and inhibits tumor forming ability
,Palanichamy, K;Kanji, S;Gordon, N;Thirumoorthy, K;Jacob, JR;Litzenberg, KT;Patel, D;Chakravarti, A;,
Clin. Cancer Res.
,PubMed ID 27810903
[NNMT]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200641L1 | Lenti ORF clone of Human nicotinamide N-methyltransferase (NNMT), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200641L2 | Lenti ORF clone of Human nicotinamide N-methyltransferase (NNMT), mGFP tagged |
CNY 5,890.00 |
|
| RC200641L3 | Lenti ORF clone of Human nicotinamide N-methyltransferase (NNMT), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200641L4 | Lenti ORF clone of Human nicotinamide N-methyltransferase (NNMT), mGFP tagged |
CNY 5,890.00 |
|
| RG200641 | NNMT (tGFP-tagged) - Human nicotinamide N-methyltransferase (NNMT) |
CNY 4,000.00 |
|
| SC321591 | NNMT (untagged)-Human nicotinamide N-methyltransferase (NNMT) |
CNY 2,400.00 |
