ATP citrate lyase (ACLY) (NM_001096) Human Tagged Lenti ORF Clone
CAT#: RC200508L3
- LentiORF®
Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, Myc-DDK-tagged
"NM_001096" in other vectors (6)
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CNY 10,064.00
CNY 1,999.00
CNY 2,700.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACL; ATPCL; CLATP |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200508).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001096 |
ORF Size | 3303 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001096.2 |
RefSeq Size | 4450 bp |
RefSeq ORF | 3306 bp |
Locus ID | 47 |
UniProt ID | P53396 |
Domains | CoA_binding, ligase-CoA |
Protein Families | Druggable Genome |
Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways |
MW | 120.8 kDa |
Gene Summary | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200508 | ACLY (Myc-DDK-tagged)-Human ATP citrate lyase (ACLY), transcript variant 1 |
CNY 7,664.00 |
|
RC200508L1 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, Myc-DDK-tagged |
CNY 10,064.00 |
|
RC200508L2 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, mGFP tagged |
CNY 11,310.00 |
|
RC200508L4 | Lenti ORF clone of Human ATP citrate lyase (ACLY), transcript variant 1, mGFP tagged |
CNY 10,064.00 |
|
RG200508 | ACLY (tGFP-tagged) - Human ATP citrate lyase (ACLY), transcript variant 1 |
CNY 9,264.00 |
|
SC119485 | ACLY (untagged)-Human ATP citrate lyase (ACLY), transcript variant 1 |
CNY 7,672.00 |
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