ARF4L (ARL4D) (NM_001661) Human Tagged ORF Clone

CAT#: RC200502

ARL4D (Myc-DDK-tagged)-Human ADP-ribosylation factor-like 4D (ARL4D)

ORF Plasmid: DDK tGFP



  "NM_001661" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ARL4D Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ARF4L; ARL6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200502 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAACCACTTGACTGAGATGGCGCCCACTGCCTCCTCCTTCTTGCCCCACTTCCAAGCCCTGCATG
TCGTGGTCATTGGGCTGGACTCTGCTGGAAAGACCTCCCTCCTTTACCGCCTCAAGTTCAAGGAGTTTGT
CCAGAGTGTCCCCACCAAAGGCTTCAACACCGAGAAGATCCGGGTGCCCCTCGGGGGATCGCGTGGCATC
ACCTTCCAAGTGTGGGACGTCGGGGGGCAGGAGAAGCTGCGACCACTGTGGCGCTCTTATACCCGCCGGA
CAGACGGTCTAGTGTTTGTGGTGGACGCTGCGGAGGCTGAGCGGCTGGAGGAAGCCAAGGTGGAGTTGCA
CCGAATCAGCCGGGCCTCGGACAACCAGGGCGTGCCAGTGCTGGTGCTGGCCAACAAGCAGGACCAGCCC
GGGGCACTGAGCGCTGCTGAGGTGGAGAAGAGGCTGGCAGTCCGAGAGCTAGCAGCCGCCACTCTCACTC
ATGTGCAAGGCTGCAGCGCTGTGGACGGTCTGGGCCTGCAGCAGGGCCTTGAGCGCCTCTATGAGATGAT
CCTCAAGAGGAAGAAGGCAGCTCGGGGTGGCAAGAAGAGACGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200502 protein sequence
Red=Cloning site Green=Tags(s)

MGNHLTEMAPTASSFLPHFQALHVVVIGLDSAGKTSLLYRLKFKEFVQSVPTKGFNTEKIRVPLGGSRGI
TFQVWDVGGQEKLRPLWRSYTRRTDGLVFVVDAAEAERLEEAKVELHRISRASDNQGVPVLVLANKQDQP
GALSAAEVEKRLAVRELAAATLTHVQGCSAVDGLGLQQGLERLYEMILKRKKAARGGKKRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001661
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001661.4
RefSeq Size 1602 bp
RefSeq ORF 606 bp
Locus ID 379
UniProt ID P49703
Domains RAS, RAB, SAR, ARF, arf
MW 22.2 kDa
Gene Summary ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS). [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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