ARF4L (ARL4D) (NM_001661) Human Tagged ORF Clone
CAT#: RC200502
ARL4D (Myc-DDK-tagged)-Human ADP-ribosylation factor-like 4D (ARL4D)
ORF Plasmid: tGFP
"NM_001661" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ARF4L; ARL6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200502 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACCACTTGACTGAGATGGCGCCCACTGCCTCCTCCTTCTTGCCCCACTTCCAAGCCCTGCATG TCGTGGTCATTGGGCTGGACTCTGCTGGAAAGACCTCCCTCCTTTACCGCCTCAAGTTCAAGGAGTTTGT CCAGAGTGTCCCCACCAAAGGCTTCAACACCGAGAAGATCCGGGTGCCCCTCGGGGGATCGCGTGGCATC ACCTTCCAAGTGTGGGACGTCGGGGGGCAGGAGAAGCTGCGACCACTGTGGCGCTCTTATACCCGCCGGA CAGACGGTCTAGTGTTTGTGGTGGACGCTGCGGAGGCTGAGCGGCTGGAGGAAGCCAAGGTGGAGTTGCA CCGAATCAGCCGGGCCTCGGACAACCAGGGCGTGCCAGTGCTGGTGCTGGCCAACAAGCAGGACCAGCCC GGGGCACTGAGCGCTGCTGAGGTGGAGAAGAGGCTGGCAGTCCGAGAGCTAGCAGCCGCCACTCTCACTC ATGTGCAAGGCTGCAGCGCTGTGGACGGTCTGGGCCTGCAGCAGGGCCTTGAGCGCCTCTATGAGATGAT CCTCAAGAGGAAGAAGGCAGCTCGGGGTGGCAAGAAGAGACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200502 protein sequence
Red=Cloning site Green=Tags(s) MGNHLTEMAPTASSFLPHFQALHVVVIGLDSAGKTSLLYRLKFKEFVQSVPTKGFNTEKIRVPLGGSRGI TFQVWDVGGQEKLRPLWRSYTRRTDGLVFVVDAAEAERLEEAKVELHRISRASDNQGVPVLVLANKQDQP GALSAAEVEKRLAVRELAAATLTHVQGCSAVDGLGLQQGLERLYEMILKRKKAARGGKKRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001661 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001661.4 |
RefSeq Size | 1602 bp |
RefSeq ORF | 606 bp |
Locus ID | 379 |
UniProt ID | P49703 |
Domains | RAS, RAB, SAR, ARF, arf |
MW | 22.2 kDa |
Gene Summary | ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS). [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200502L3 | Lenti ORF clone of Human ADP-ribosylation factor-like 4D (ARL4D), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200502L4 | Lenti ORF clone of Human ADP-ribosylation factor-like 4D (ARL4D), mGFP tagged |
CNY 5,890.00 |
|
RG200502 | ARL4D (tGFP-tagged) - Human ADP-ribosylation factor-like 4D (ARL4D) |
CNY 4,000.00 |
|
SC119093 | ARL4D (untagged)-Human ADP-ribosylation factor-like 4D (ARL4D) |
CNY 2,400.00 |