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Chromogranin A (CHGA) (NM_001275) Human Tagged ORF Clone
CAT＃: RC200492
CHGA (Myc-DDK-tagged)-Human chromogranin A (parathyroid secretory protein 1) (CHGA)
ORF Plasmid: tGFP
"NM_001275" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200492 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCCCTGTGAACAGCC CTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCATCTCCGACACACTTTCCAAGCC CAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACACTCCGAGGAGATGAACGGATCCTTTCCATTCTG AGACATCAGAATTTACTGAAGGAGCTCCAAGACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGC AGAAGAAACACAGCGGTTTTGAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCT GAAAGAGGCGGTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAGAG AAAAGTGGTGAAGCCACAGACGGAGCCAGGCCCCAGGCCCTCCCGGAGCCCATGCAGGAGTCCAAGGCTG AGGGGAACAATCAGGCCCCTGGGGAGGAAGAGGAGGAGGAGGAGGAGGCCACCAACACCCACCCTCCAGC CAGCCTCCCCAGCCAGAAATACCCAGGCCCACAGGCCGAGGGGGACAGTGAGGGCCTCTCTCAGGGTCTG GTGGACAGAGAGAAGGGCCTGAGTGCAGAGCCAGGGTGGCAGGCAAAGAGAGAAGAGGAGGAGGAGGAGG AGGAGGAGGCTGAGGCTGGAGAGGAGGCTGTCCCCGAGGAAGAAGGCCCCACTGTAGTGCTGAACCCCCA CCCGAGCCTTGGCTACAAGGAGATCCGGAAAGGCGAGAGTCGGTCGGAGGCTCTGGCTGTGGATGGAGCT GGGAAGCCTGGGGCTGAGGAGGCTCAGGACCCCGAAGGGAAGGGAGAACAGGAGCACTCCCAGCAGAAAG AGGAGGAGGAGGAGATGGCAGTGGTCCCGCAAGGCCTCTTCCGGGGTGGGAAGAGCGGAGAGCTGGAGCA GGAGGAGGAGCGGCTCTCCAAGGAGTGGGAGGACTCCAAACGCTGGAGCAAGATGGACCAGCTGGCCAAG GAGCTGACGGCTGAGAAGCGGCTGGAGGGGCAGGAGGAGGAGGAGGACAACCGGGACAGTTCCATGAAGC TCTCCTTCCGGGCCCGGGCCTACGGCTTCAGGGGCCCTGGGCCGCAGCTGCGACGAGGCTGGAGGCCATC CTCCCGGGAGGACAGCCTTGAGGCGGGCCTGCCCCTCCAGGTCCGAGGCTACCCCGAGGAGAAGAAAGAG GAGGAGGGCAGCGCAAACCGCAGACCAGAGGACCAGGAGCTGGAGAGCCTGTCGGCCATTGAGGCAGAGC TGGAGAAAGTGGCCCACCAGCTGCAGGCACTACGGCGGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200492 protein sequence
Red=Cloning site Green=Tags(s) MRSAAVLALLLCAGQVTALPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSIL RHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAE KSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGL VDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGA GKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAK ELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKE EEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001275 |
| ORF Size | 1371 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001275.4 |
| RefSeq Size | 2079 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 1113 |
| UniProt ID | P10645 |
| Domains | Granin |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 50.7 kDa |
| Gene Summary | The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Histamine via histamine H1 receptor enhances the muscarinic receptor‐induced calcium response to acetylcholine in an enterochromaffin cell model
,null,
Clinical and Experimental Pharmacology & Physiology
,PubMed ID 35652717
[Chromogranin A]
|
|
Chromogranin A regulates neuroblastoma proliferation and phenotype
,Zhang, D;Babayan, L;Ho, H;Heaney, AP;,
Biol Open
,PubMed ID 30833285
[Chromogranin A]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200492L1 | Lenti ORF clone of Human chromogranin A (parathyroid secretory protein 1) (CHGA), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200492L2 | Lenti ORF clone of Human chromogranin A (parathyroid secretory protein 1) (CHGA), mGFP tagged |
CNY 5,890.00 |
|
| RC200492L3 | Lenti ORF clone of Human chromogranin A (parathyroid secretory protein 1) (CHGA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200492L4 | Lenti ORF clone of Human chromogranin A (parathyroid secretory protein 1) (CHGA), mGFP tagged |
CNY 5,890.00 |
|
| RG200492 | CHGA (tGFP-tagged) - Human chromogranin A (parathyroid secretory protein 1) (CHGA) |
CNY 5,256.00 |
|
| SC119356 | CHGA (untagged)-Human chromogranin A (parathyroid secretory protein 1) (CHGA) |
CNY 3,656.00 |
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