DHCR7 (NM_001360) Human Tagged Lenti ORF Clone
CAT#: RC200480L3
- LentiORF®
Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, Myc-DDK-tagged
"NM_001360" in other vectors (6)
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CNY 6,056.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SLOS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200480).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001360 |
ORF Size | 1425 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001360.2 |
RefSeq Size | 2665 bp |
RefSeq ORF | 1428 bp |
Locus ID | 1717 |
UniProt ID | Q9UBM7 |
Domains | ERG4_ERG24 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Metabolic pathways, Steroid biosynthesis |
MW | 54.5 kDa |
Gene Summary | This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200480 | DHCR7 (Myc-DDK-tagged)-Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1 |
CNY 3,656.00 |
|
RC200480L1 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC200480L2 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC200480L4 | Lenti ORF clone of Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG200480 | DHCR7 (tGFP-tagged) - Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1 |
CNY 5,256.00 |
|
SC110871 | DHCR7 (untagged)-Human 7-dehydrocholesterol reductase (DHCR7), transcript variant 1 |
CNY 3,656.00 |