EIPR1 (NM_003310) Human Tagged ORF Clone

CAT#: RC200409

TSSC1 (Myc-DDK-tagged)-Human tumor suppressing subtransferable candidate 1 (TSSC1)

ORF Plasmid: DDK tGFP



  "NM_003310" in other vectors (4)

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CNY 3,656.00

CNY 5,320.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TSSC1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms EIPR-1; TSSC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200409 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACGATGCACCAGTGATCTACGGGCTGGAGTTCCAGGCACGTGCCTTAACACCTCAAACTGCAG
AAACAGATGCCATTCGGTTTTTGGTTGGGACGCAGTCTCTTAAATATGATAATCAGATCCATATCATAGA
TTTTGACGATGAAAACAACATTATAAATAAAAATGTCCTCCTCCATCAAGCGGGTGAAATCTGGCATATT
AGCGCTAGCCCTGCAGACAGAGGTGTGCTGACGACCTGCTACAACAGAACTTCAGACAGCAAAGTCCTGA
CATGTGCAGCCGTGTGGAGGATGCCGAAGGAATTGGAATCAGGCAGCCACGAGTCCCCTGATGATTCATC
CAGCACTGCACAGACCCTGGAGCTGCTCTGTCACCTTGACAACACAGCCCATGGCAACATGGCCTGTGTC
GTGTGGGAGCCAATGGGAGATGGGAAGAAAATCATTTCCTTGGCTGATAACCATATCCTGCTGTGGGATT
TACAGGAAAGCTCGAGCCAGGCTGTGCTGGCCAGCTCAGCGTCCCTGGAAGGGAAGGGACAACTGAAGTT
CACCTCAGGACGGTGGAGCCCACATCATAACTGCACCCAGGTGGCCACAGCGAACGACACCACCCTCCGT
GGCTGGGACACCCGGAGCATGAGCCAGATCTACTGCATAGAGAATGCCCACGGACAGCTGGTGCGGGACC
TTGACTTTAATCCCAATAAGCAGTACTACTTGGCCAGCTGCGGAGACGACTGTAAGGTGAAGTTCTGGGA
CACCCGAAATGTCACCGAACCCGTGAAGACCCTGGAGGAGCACTCCCACTGGGTGTGGAACGTCCGCTAC
AACCACTCTCATGACCAGCTGGTCCTCACGGGCAGCAGTGACAGCAGAGTCATCCTTTCCAACATGGTGT
CCATCTCGTCGGAGCCCTTCGGCCACTTGGTAGACGACGATGACATCAGTGACCAGGAGGACCACCGTTC
TGAAGAGAAGAGCAAGGAGCCCCTGCAGGACAACGTGATCGCCACCTACGAGGAGCACGAGGACAGCGTC
TATGCCGTGGACTGGTCCTCGGCTGACCCGTGGCTGTTTGCCTCCCTGAGCTATGACGGGAGGCTCGTGA
TCAACAGGGTGCCCAGGGCCCTGAAGTACCACATCCTGCTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200409 protein sequence
Red=Cloning site Green=Tags(s)

MEDDAPVIYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINKNVLLHQAGEIWHI
SASPADRGVLTTCYNRTSDSKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMACV
VWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLEGKGQLKFTSGRWSPHHNCTQVATANDTTLR
GWDTRSMSQIYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDTRNVTEPVKTLEEHSHWVWNVRY
NHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEHEDSV
YAVDWSSADPWLFASLSYDGRLVINRVPRALKYHILL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003310
ORF Size 1161 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003310.5
RefSeq Size 1732 bp
RefSeq ORF 1164 bp
Locus ID 7260
UniProt ID Q53HC9
Domains WD40
Protein Families Druggable Genome
MW 43.6 kDa
Gene Summary This gene has been reported in PMID 9403053 as one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alignment of this gene to genomic sequence data suggests that this gene resides on chromosome 2 rather than chromosome 11. [provided by RefSeq, Dec 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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