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TBCD (NM_005993) Human Tagged ORF Clone
CAT＃: RC200381
- TrueORF®
TBCD (Myc-DDK-tagged)-Human tubulin folding cofactor D (TBCD)
ORF Plasmid: tGFP
"NM_005993" in other vectors (4)
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CNY 7,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PEBAT; SSD-1; tfcD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200381 representing NM_005993
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGAGCGACGAACCGGCCGCGGGCGGCCCCGAGGAGGAGGCGGAGGACGAGACACTGGCCTTTG GCGCGGCGCTGGAAGCGTTCGGCGAGAGCGCGGAGACCCGGGCGCTGCTGGGCCGCCTGCGGGAGGTGCA CGGCGGCGGCGCGGAGCGCGAGGTGGCCCTGGAGCGGTTCCGCGTAATAATGGACAAATACCAGGAGCAG CCTCATCTGTTGGACCCGCACCTTGAATGGATGATGAACTTGTTGTTGGACATAGTGCAAGATCAGACAT CTCCAGCTTCCCTTGTACATCTGGCTTTTAAATTTCTTTACATCATCACCAAGGTTCGAGGCTATAAAAC ATTTCTTCGTTTATTTCCTCATGAAGTTGCCGATGTAGAGCCTGTTTTAGATTTGGTCACAATTCAGAAT CCCAAGGACCATGAAGCTTGGGAAACCCGCTACATGCTTTTGCTCTGGCTCTCCGTGACCTGCCTGATCC CTTTTGATTTTTCTCGCCTTGACGGGAACCTCCTCACCCAGCCTGGGCAAGCACGAATGTCCATAATGGA CCGTATTCTCCAAATAGCAGAGTCCTACTTGATTGTCAGTGACAAGGCCCGAGATGCAGCTGCTGTCCTT GTGTCCAGATTTATCACACGTCCTGATGTCAAGCAAAGCAAGATGGCTGAGTTCCTGGACTGGAGCCTGT GCAATCTGGCCCGTTCCTCCTTCCAGACCATGCAGGGGGTCATCACCATGGATGGGACGCTGCAGGCCCT GGCACAAATATTTAAACATGGAAAACGTGAAGACTGTTTGCCCTATGCTGCCACTGTCCTCAGGTGCCTC GATGGCTGCAGACTCCCTGAGAGCAACCAGACCCTGCTGCGGAAGCTGGGGGTGAAGCTTGTGCAGCGAC TGGGGCTGACATTCCTGAAGCCGAAGGTGGCAGCATGGAGGTACCAGCGTGGCTGCCGATCTTTGGCTGC AAATCTGCAGCTCCTCACTCAGGGTCAGAGTGAGCAGAAGCCACTCATCCTGACCGAAGATGACGACGAA GATGACGACGTCCCAGAGGGGGTGGAGCGTGTGATAGAGCAGCTGCTGGTCGGGCTGAAGGACAAGGACA CGGTCGTGCGGTGGTCTGCAGCCAAGGGCATCGGTAGGATGGCTGGCAGGCTTCCCAGAGCCCTGGCGGA TGATGTGGTCGGGTCTGTGCTGGACTGCTTCAGTTTCCAGGAGACTGACAAGGCGTGGCATGGGGGATGT CTGGCGCTGGCAGAGCTGGGCAGGAGAGGCCTGTTGCTGCCGTCTCGACTCGTGGATGTTGTCGCCGTGA TCCTGAAGGCGCTGACCTACGACGAGAAGCGGGGTGCCTGCAGCGTGGGCACCAACGTCAGGGACGCCGC CTGCTACGTGTGCTGGGCCTTCGCGCGTGCCTATGAGCCTCAGGAGCTGAAGCCCTTTGTGACTGCAATC TCGAGTGCACTGGTGATTGCTGCGGTGTTTGACCGAGACATAAACTGCAGAAGAGCAGCCTCTGCCGCCT TCCAGGAGAATGTGGGGAGACAGGGCACTTTCCCTCATGGTATTGATATTTTGACCACAGCTGACTATTT TGCCGTCGGTAACAGATCCAACTGTTTCCTGGTTATAAGTGTGTTTATTGCCGGCTTTCCTGAGTACACG CAGCCAATGATAGACCACCTGGTTACCATGAAGATCAGCCACTGGGATGGGGTCATCCGAGAGTTGGCTG CGAGGGCGCTGCACAACCTGGCCCAGCAGGCACCCGAGTTCAGCGCCACGCAAGTCTTCCCGAGGCTGCT GTCCATGACACTGAGTCCAGATCTTCACATGAGGCATGGGTCGATTCTCGCCTGCGCAGAAGTTGCTTAC GCCTTGTACAAACTTGCAGCCCAAGAGAACAGGCCCGTCACGGACCATCTGGACGAGCAGGCAGTGCAGG GCCTGAAGCAGATTCACCAGCAGCTCTATGATCGTCAGTTATACAGGGGTCTGGGAGGACAGCTCATGAG ACAAGCAGTGTGTGTTTTAATAGAAAAGTTGTCACTTTCCAAAATGCCCTTTAGAGGTGACACCGTAATT GATGGTTGGCAATGGCTGATAAATGACACTTTGAGACATCTCCATCTCATCTCAAGTCACTCCCGCCAGC AGATGAAGGATGCAGCAGTCTCGGCCCTGGCTGCTCTATGCAGTGAATATTACATGAAGGAGCCGGGGGA GGCAGATCCCGCAATTCAGGAGGAGCTGATCACGCAGTACCTGGCTGAGCTTCGGAACCCCGAGGAGATG ACTCGCTGTGGCTTCTCGTTGGCCTTGGGCGCCCTTCCAGGCTTCCTTCTGAAAGGCCGGCTCCAGCAGG TTCTCACAGGTTTAAGAGCAGTTACCCACACTTCCCCCGAGGACGTAAGTTTTGCTGAGTCCAGGAGAGA CGGCTTGAAGGCCATTGCGAGGATTTGCCAGACTGTTGGTGTGAAAGCAGGAGCCCCAGACGAAGCTGTG TGCGGAGAGAATGTTTCCCAGATTTACTGTGCGCTGCTGGGCTGCATGGACGACTACACCACGGACAGCA GAGGGGACGTGGGCACCTGGGTCCGCAAGGCCGCCATGACCAGTCTGATGGATCTGACACTTCTGCTGGC TCGGAGCCAGCCTGAGCTGATCGAGGCCCATACCTGTGAGCGCATCATGTGCTGTGTGGCCCAGCAGGCC AGTGAGAAGATTGACCGTTTCCGTGCTCACGCCGCCAGCGTGTTCCTGACGCTCCTGCACTTTGACAGCC CTCCCATCCCCCACGTGCCCCACCGAGGAGAACTGGAAAAGCTGTTTCCCAGGTCCGATGTGGCCTCCGT GAACTGGAGTGCACCTTCCCAGGCCTTCCCACGCATCACCCAGCTCCTTGGGCTGCCCACCTACCGCTAC CACGTCCTGCTGGGGCTAGTCGTGTCCCTGGGCGGCTTGACGGAGTCGACGATCCGGCACTCCACCCAGA GCCTCTTTGAGTACATGAAGGGCATTCAGAGCGACCCGCAGGCCCTGGGCAGCTTCAGCGGGACCCTTCT GCAGATCTTTGAGGACAACCTTCTGAATGAGAGGGTGTCCGTGCCGCTGCTGAAGACGCTGGACCACGTG CTCACCCACGGCTGCTTCGACATCTTCACCACGGAGGAGGACCACCCCTTTGCTGTGAAGTTGCTTGCGC TCTGTAAGAAAGAAATCAAGAATTCAAAAGATATCCAGAAGCTCCTGTCAGGCATCGCAGTGTTCTGCGA GATGGTGCAGTTCCCCGGCGACGTGAGGAGGCAGGCCCTCCTGCAGCTGTGTCTGCTCCTCTGCCACCGT TTCCCGCTGATCCGGAAGACCACGGCCAGCCAGGTGTACGAGACATTGCTCACCTACAGTGACGTCGTGG GCGCGGATGTGCTGGACGAGGTGGTGACTGTGCTCAGTGACACTGCGTGGGACGCGGAGCTTGCAGTGGT GAGAGAGCAGCGCAACCGTCTGTGTGACCTTCTGGGCGTACCCAGGCCCCAGCTGGTGCCCCAGCCTGGT GCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200381 representing NM_005993
Red=Cloning site Green=Tags(s) MALSDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAEREVALERFRVIMDKYQEQ PHLLDPHLEWMMNLLLDIVQDQTSPASLVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQN PKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQARMSIMDRILQIAESYLIVSDKARDAAAVL VSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCLPYAATVLRCL DGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANLQLLTQGQSEQKPLILTEDDDE DDDVPEGVERVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGC LALAELGRRGLLLPSRLVDVVAVILKALTYDEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAI SSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISVFIAGFPEYT QPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAY ALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVI DGWQWLINDTLRHLHLISSHSRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEM TRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVSFAESRRDGLKAIARICQTVGVKAGAPDEAV CGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCERIMCCVAQQA SEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRY HVLLGLVVSLGGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERVSVPLLKTLDHV LTHGCFDIFTTEEDHPFAVKLLALCKKEIKNSKDIQKLLSGIAVFCEMVQFPGDVRRQALLQLCLLLCHR FPLIRKTTASQVYETLLTYSDVVGADVLDEVVTVLSDTAWDAELAVVREQRNRLCDLLGVPRPQLVPQPG AC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005993 |
| ORF Size | 3576 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005993.5 |
| RefSeq Size | 4449 bp |
| RefSeq ORF | 3579 bp |
| Locus ID | 6904 |
| UniProt ID | Q9BTW9 |
| Domains | B-tub_coD |
| MW | 132.4 kDa |
| Gene Summary | Cofactor D is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Biallelic Mutations in TBCD, Encoding the Tubulin Folding Cofactor D, Perturb Microtubule Dynamics and Cause Early-Onset Encephalopathy
,Flex, E;Niceta, M;Cecchetti, S;Thiffault, I;Au, MG;Capuano, A;Piermarini, E;Ivanova, AA;Francis, JW;Chillemi, G;Chandramouli, B;Carpentieri, G;Haaxma, CA;Ciolfi, A;Pizzi, S;Douglas, GV;Levine, K;Sferra, A;Dentici, ML;Pfundt, RR;Le Pichon, JB;Farrow, E;Baas, F;Piemonte, F;Dallapiccola, B;Graham, JM;Saunders, CJ;Bertini, E;Kahn, RA;Koolen, DA;Tartaglia, M;,
Am. J. Hum. Genet.
,PubMed ID 27666370
[TBCD]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200381L3 | Lenti-ORF clone of TBCD (Myc-DDK-tagged)-Human tubulin folding cofactor D (TBCD) |
CNY 12,070.00 |
|
| RC200381L4 | Lenti-ORF clone of TBCD (mGFP-tagged)-Human tubulin folding cofactor D (TBCD) |
CNY 12,070.00 |
|
| RG200381 | TBCD (tGFP-tagged) - Human tubulin folding cofactor D (TBCD) |
CNY 9,904.00 |
|
| SC321348 | TBCD (untagged)-Human tubulin folding cofactor D (TBCD) |
CNY 8,304.00 |
