United States
Germany
Japan
United Kingdom
China
Cathepsin H (CTSH) (NM_004390) Human Tagged ORF Clone
CAT＃: RC200373
CTSH (Myc-DDK-tagged)-Human cathepsin H (CTSH)
ORF Plasmid: tGFP
"NM_004390" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 5,320.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACC-4; ACC-5; ACC4; ACC5; CPSB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200373 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGTGGGCCACGCTGCCGCTGCTCTGCGCCGGGGCCTGGCTCCTGGGAGTCCCCGTCTGCGGTGCCGCCG AACTGTCCGTGAACTCCTTAGAGAAGTTTCACTTCAAGTCATGGATGTCTAAGCACCGTAAGACCTACAG TACGGAGGAGTACCACCACAGGCTGCAGACGTTTGCCAGCAACTGGAGGAAGATAAACGCCCACAACAAT GGGAACCACACATTTAAAATGGCACTGAACCAATTTTCAGACATGAGCTTTGCTGAAATAAAACACAAGT ATCTCTGGTCAGAGCCTCAGAATTGCTCAGCCACCAAAAGTAACTACCTTCGAGGTACTGGTCCCTACCC ACCTTCCGTGGACTGGCGGAAAAAAGGAAATTTTGTCTCACCTGTGAAAAATCAGGGTGCCTGCGGCAGT TGCTGGACTTTCTCCACCACTGGGGCCCTGGAGTCTGCGATCGCCATCGCAACCGGAAAGATGCTGTCCT TGGCGGAACAGCAGCTGGTGGACTGCGCCCAGGACTTCAATAATCACGGCTGCCAAGGGGGTCTCCCCAG CCAGGCTTTCGAGTATATCCTGTACAACAAGGGGATCATGGGTGAAGACACCTACCCCTACCAGGGCAAG GATGGTTATTGCAAGTTCCAACCTGGAAAGGCCATCGGCTTTGTCAAGGATGTAGCCAACATCACAATCT ATGACGAGGAAGCGATGGTGGAGGCTGTGGCCCTCTACAACCCTGTGAGCTTTGCCTTTGAGGTGACTCA GGACTTCATGATGTATAGAACGGGCATCTACTCCAGTACTTCCTGCCATAAAACTCCAGATAAAGTAAAC CATGCAGTACTTGCTGTTGGGTATGGAGAAAAAAATGGGATCCCTTACTGGATCGTGAAAAACTCTTGGG GTCCCCAGTGGGGAATGAACGGGTACTTCCTCATCGAGCGCGGAAAGAACATGTGTGGCCTGGCTGCCTG CGCCTCCTACCCCATCCCTCTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200373 protein sequence
Red=Cloning site Green=Tags(s) MWATLPLLCAGAWLLGVPVCGAAELSVNSLEKFHFKSWMSKHRKTYSTEEYHHRLQTFASNWRKINAHNN GNHTFKMALNQFSDMSFAEIKHKYLWSEPQNCSATKSNYLRGTGPYPPSVDWRKKGNFVSPVKNQGACGS CWTFSTTGALESAIAIATGKMLSLAEQQLVDCAQDFNNHGCQGGLPSQAFEYILYNKGIMGEDTYPYQGK DGYCKFQPGKAIGFVKDVANITIYDEEAMVEAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVN HAVLAVGYGEKNGIPYWIVKNSWGPQWGMNGYFLIERGKNMCGLAACASYPIPLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004390 |
| ORF Size | 1005 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004390.5 |
| RefSeq Size | 1494 bp |
| RefSeq ORF | 1008 bp |
| Locus ID | 1512 |
| UniProt ID | P09668 |
| Domains | Pept_C1 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Lysosome |
| MW | 37.4 kDa |
| Gene Summary | The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Crystal structures of human procathepsin H
,Hao, Y;Purtha, W;Cortesio, C;Rui, H;Gu, Y;Chen, H;Sickmier, EA;Manzanillo, P;Huang, X;,
PLoS ONE
,PubMed ID 30044821
[Cathepsin H]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200373L1 | Lenti ORF clone of Human cathepsin H (CTSH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200373L2 | Lenti ORF clone of Human cathepsin H (CTSH), mGFP tagged |
CNY 5,890.00 |
|
| RC200373L3 | Lenti ORF clone of Human cathepsin H (CTSH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200373L4 | Lenti ORF clone of Human cathepsin H (CTSH), mGFP tagged |
CNY 5,890.00 |
|
| RG200373 | CTSH (tGFP-tagged) - Human cathepsin H (CTSH) |
CNY 4,370.00 |
|
| SC319365 | CTSH (untagged)-Human cathepsin H (CTSH) |
CNY 2,950.00 |
其它Cathepsin H产品
