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NT5C2 (NM_012229) Human Tagged ORF Clone
CAT＃: RC200194
NT5C2 (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1
ORF Plasmid: tGFP
"NM_012229" in other vectors (4)
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CNY 4,184.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cN-II; GMP; NT5B; PNT5; SPG45; SPG65 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAACCTCCTGGAGTGATCGGTTACAGAATGCAGCAGATATGCCTGCTAACATGGATAAGCATGCCC TGAAAAAGTATCGTCGAGAAGCCTATCATCGGGTGTTTGTGAACCGAAGTTTAGCAATGGAAAAGATAAA GTGTTTTGGTTTTGATATGGATTATACCCTTGCTGTGTACAAGTCCCCAGAGTATGAGTCCCTTGGTTTT GAGCTTACTGTGGAGAGATTAGTTTCTATTGGCTATCCCCAGGAGTTGCTCAGCTTTGCTTATGATTCTA CATTCCCTACCAGGGGACTTGTCTTTGACACACTGTATGGAAATCTTTTGAAAGTCGATGCCTATGGAAA CCTCTTGGTCTGTGCACATGGATTTAACTTTATAAGGGGACCAGAAACTAGAGAACAGTATCCAAATAAA TTTATCCAGCGAGATGATACTGAAAGATTTTACATTCTGAACACACTATTCAACCTACCAGAGACCTACC TGTTGGCCTGCCTAGTAGATTTTTTTACTAATTGTCCCAGATATACCAGTTGTGAAACAGGATTTAAAGA TGGGGACCTCTTCATGTCCTACCGGAGTATGTTCCAGGATGTAAGAGATGCTGTTGACTGGGTTCATTAC AAGGGCTCCCTTAAGGAAAAGACAGTTGAAAATCTTGAGAAGTATGTAGTCAAAGATGGAAAACTGCCTT TGCTTCTGAGCCGGATGAAGGAAGTAGGGAAAGTATTTCTTGCTACCAACAGTGACTATAAATATACAGA TAAAATTATGACTTACCTGTTTGACTTCCCACATGGCCCCAAGCCTGGGAGCTCCCATCGACCATGGCAG TCCTACTTTGACTTGATCTTGGTGGATGCACGGAAACCACTCTTTTTTGGAGAAGGCACAGTACTGCGTC AGGTGGATACTAAAACTGGCAAGCTGAAAATTGGTACCTACACAGGGCCCCTACAGCATGGTATCGTCTA CTCAGGAGGTTCTTCTGATACGATCTGTGACCTGTTGGGAGCCAAGGGAAAAGACATTTTGTATATTGGA GATCACATTTTTGGGGACATTTTAAAATCAAAGAAACGGCAAGGGTGGCGAACTTTTTTGGTGATTCCTG AACTCGCACAGGAGCTACATGTCTGGACTGACAAGAGTTCACTTTTCGAAGAACTTCAGAGCTTGGATAT TTTCTTGGCTGAACTCTACAAGCATCTTGACAGCAGTAGCAATGAGCGTCCAGACATCAGTTCCATCCAG AGACGTATTAAGAAAGTAACTCATGACATGGACATGTGCTATGGGATGATGGGAAGCCTGTTTCGCAGTG GCTCCCGGCAGACCCTTTTTGCCAGTCAAGTGATGCGTTATGCTGACCTCTATGCAGCATCTTTCATCAA CCTGCTGTATTACCCTTTCAGCTACCTCTTCAGGGCTGCCCATGTCTTGATGCCTCATGAATCAACGGTG GAGCACACACACGTAGATATCAATGAGATGGAGTCTCCTCTTGCCACCCGGAACCGCACATCAGTGGATT TCAAAGACACTGACTACAAGCGGCACCAGCTGACACGGTCAATTAGTGAGATTAAACCTCCCAACCTCTT CCCACTGGCCCCCCAGGAAATTACACACTGCCATGACGAAGATGATGATGAAGAGGAGGAGGAGGAGGAA GAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200194 protein sequence
Red=Cloning site Green=Tags(s) MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGF ELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNK FIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHY KGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQ SYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIG DHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQ RRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTV EHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEE E TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012229 |
| ORF Size | 1686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012229.4 |
| RefSeq Size | 3551 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 22978 |
| UniProt ID | P49902 |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
| MW | 65 kDa |
| Gene Summary | This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200194L3 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200194L4 | Lenti ORF clone of Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200194 | NT5C2 (tGFP-tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1 |
CNY 5,784.00 |
|
| SC310267 | NT5C2 (untagged)-Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1 |
CNY 4,184.00 |
