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SWAP70 (NM_015055) Human Tagged ORF Clone
CAT＃: RC200074
SWAP70 (Myc-DDK-tagged)-Human SWAP switching B-cell complex 70kDa subunit (SWAP70)
ORF Plasmid: tGFP
"NM_015055" in other vectors (4)
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CNY 4,360.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSPC321; SWAP-70 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200074 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCTTGAAGGAGGAGCTGCTCAAAGCCATCTGGCACGCCTTCACCGCACTCGACCAGGACCACA GCGGCAAGGTCTCCAAGTCCCAGCTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCTCA TGACCCAGTTGCCCTTGAAGAGCACTTCAGGGATGATGATGAGGGTCCAGTGTCCAACCAGGGCTACATG CCTTATTTAAACAGGTTCATTTTGGAAAAGGTCCAAGACAACTTTGACAAGATTGAATTCAATAGGATGT GTTGGACCCTCTGTGTCAAAAAAAACCTCACAAAGAATCCCCTGCTCATTACAGAAGAAGATGCATTTAA AATATGGGTTATTTTCAACTTTTTATCTGAGGACAAGTATCCATTAATTATTGTGTCAGAAGAGATTGAA TACCTGCTTAAGAAGCTTACAGAAGCTATGGGAGGAGGTTGGCAGCAAGAACAATTTGAACATTATAAAA TCAACTTTGATGACAGTAAAAATGGCCTTTCTGCATGGGAACTTATTGAGCTTATTGGAAATGGACAGTT TAGCAAAGGCATGGACCGGCAGACTGTGTCTATGGCAATTAATGAAGTCTTTAATGAACTTATATTAGAT GTGTTAAAGCAGGGTTACATGATGAAAAAGGGCCACAGACGGAAAAACTGGACTGAAAGATGGTTTGTAC TAAAACCCAACATAATTTCTTACTATGTGAGTGAGGATCTGAAGGATAAGAAAGGAGACATTCTCTTGGA TGAAAATTGCTGTGTAGAGTCCTTGCCTGACAAAGATGGAAAGAAATGCCTTTTTCTCGTAAAATGTTTT GATAAGACTTTTGAAATCAGTGCTTCAGATAAGAAGAAGAAACAGGAGTGGATTCAAGCCATTCATTCTA CTATTCATCTGTTGAAGCTGGGCAGCCCTCCACCACACAAAGAAGCCCGCCAGCGTCGGAAAGAACTCCG GAAGAAGCAGCTGGCTGAACAAGAGGAACTGGAGCGACAAATGAAGGAACTCCAGGCCGCCAACGAAAGC AAGCAGCAGGAGCTGGAGGCCGTGCGGAAGAAACTGGAGGAAGCAGCATCTCGTGCAGCAGAAGAGGAAA AGAAACGCCTTCAGACTCAAGTGGAACTTCAGGCCAGGTTCAGCACAGAGCTGGAAAGAGAGAAGCTTAT CAGACAGCAGATGGAAGAACAGGTTGCTCAAAAGTCCTCTGAACTGGAACAGTATTTACAGCGAGTACGG GAGCTGGAAGACATGTACCTAAAGCTGCAGGAGGCTCTTGAAGATGAGAGACAGGCCCGGCAAGATGAAG AGACAGTGCGGAAGCTTCAGGCCAGGTTGTTGGAGGAAGAGTCTTCCAAGAGGGCTGAACTAGAAAAGTG GCACTTGGAGCAGCAGCAGGCCATTCAGACAACCGAGGCGGAGAAGCAGGAGTTGGAGAATCAGCGTGTC CTGAAGGAACAGGCCCTGCAGGAGGCCATGGAGCAGCTGGAGCAGCTTGAGTTAGAACGGAAGCAAGCAC TTGAGCAGTACGAGGAAGTTAAAAAGAAGCTGGAGATGGCAACTAATAAGACCAAGAGCTGGAAGGACAA AGTGGCCCATCATGAAGGATTAATTCGACTGATAGAACCAGGTTCAAAGAACCCTCACCTGATCACTAAC TGGGGACCTGCAGCTTTCACTGAGGCAGAACTTGAAGAGAGAGAGAAGAACTGGAAAGAGAAAAAGACCA CGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200074 protein sequence
Red=Cloning site Green=Tags(s) MGSLKEELLKAIWHAFTALDQDHSGKVSKSQLKVLSHNLCTVLKVPHDPVALEEHFRDDDEGPVSNQGYM PYLNRFILEKVQDNFDKIEFNRMCWTLCVKKNLTKNPLLITEEDAFKIWVIFNFLSEDKYPLIIVSEEIE YLLKKLTEAMGGGWQQEQFEHYKINFDDSKNGLSAWELIELIGNGQFSKGMDRQTVSMAINEVFNELILD VLKQGYMMKKGHRRKNWTERWFVLKPNIISYYVSEDLKDKKGDILLDENCCVESLPDKDGKKCLFLVKCF DKTFEISASDKKKKQEWIQAIHSTIHLLKLGSPPPHKEARQRRKELRKKQLAEQEELERQMKELQAANES KQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVR ELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTTEAEKQELENQRV LKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLIEPGSKNPHLITN WGPAAFTEAELEEREKNWKEKKTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015055 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015055.4 |
| RefSeq Size | 4882 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 23075 |
| UniProt ID | Q9UH65 |
| MW | 69 kDa |
| Gene Summary | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200074L3 | Lenti ORF clone of Human SWAP switching B-cell complex 70kDa subunit (SWAP70), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200074L4 | Lenti ORF clone of Human SWAP switching B-cell complex 70kDa subunit (SWAP70), mGFP tagged |
CNY 6,760.00 |
|
| RG200074 | SWAP70 (tGFP-tagged) - Human SWAP switching B-cell complex 70kDa subunit (SWAP70) |
CNY 5,960.00 |
|
| SC321329 | SWAP70 (untagged)-Human SWAP switching B-cell complex 70kDa subunit (SWAP70) |
CNY 4,360.00 |
