Jmjd1c (NM_001242396) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5430433L24Rik; D630035I23Rik; Jmjdic; TRIP8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231944 representing NM_001242396 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGTCCTTATTCATTAAATGGATACAGAGTGAGAGTCTATAGACAAGATTCTGCCACCCAGTGGT TTACTGGCATAATTACTCATCATGATCTCTTCACCCGCACCATGATCGTTATGAATGATCAGGTACTAGA ACCACAGAATGTCGATCCTTCTATGGTTCAAATGACCTTTCTAGATGATGTCGTTCACTCTTTGTTAAAA GGTGAAAATATTGGCATTACATCACGGCGAAGGTCTCGTGCCAGTCAAAATATCAGCACTGTTCACGGTC ATTATACACGTGCTCAAGCAAATAGTCCCAGACCAGCAATGAACTCTCAAGCTGCTGTACCAAAACAGAA CACACACCAGCAACAGCAACAAAGAAGTATTCGTCCAAATAAGAGGAAGGGTTCAGATAGCAGCATACCC GATGAAGAGAAGATGAAGGAAGATAAATATGATTGTGTATCACGAGGAGAAAATCCTAAAGGTAAGAACA AGCACGTGGTGACAAAACGGCGGAAACCTGAGGAGGCCGAGAAGAGGCTGAGCATGAAAAGGCTCCGGAC AGACAATGCCTCTGACGCCTCCGAGAGCAGTGACGCAGAGAGCTCCAGCAAGAGAGTGACTGAGACTTCC TCCTCTGAACCAATGCCAGAGTATGAACCGAAAAATAAAGTCACCTCAAAGGTAAATGGAGAGGAGGGAC AGTCCCAGGCTGCTGAAGAGGCAGGAGAAGAGACCCTGATAGATACTCGGCCTCCCTGGGACCAGATGCA GGAAGATAAAAACCACAACGAAGGGGAGAAGCCCAAGTCCACGGACAGCCACCTTCAAGACAAAATGACT CTCCGTTCATCTGAGCAAGCCACAGTTGCTGACCACAATTCTAATGATTCAGTCCTTCAAGAATGCAATG TGGAAAACCAACGCACAGTGGAATTATTACCAAAGGACAGGTTAGTGTCCAGAACACCGACACCCAAATG TGTTACGGATATCAAGAACGACACTCATTCTGAGAGGGCGGCTCAGGAAAATTTAAATACTTTTGGCCTC CAGACACCTGAGAACATGGATCCAAATGTTAGTGATTCCAAACATTCAAATGCTAAATACCTAGAAACAG CAAAGCAAGATTGTGACCAGAGCTGGGTCAGTGATGTAGTGAAAGTGGATTTAACTCAGTCAAGTGTTAC AAATGCTCCTTCAGGAAGTGATAAGCGCGATACGGAGAAAGAGAGAAATCACTATGTCTCATACATGTCT TCATTAAGTGCAGTTTCTGTCACAGAGGATCAGCTACATAAACGAAGTCCACCCCCAGAAACTATAAAAG CTAAACTAACTACTTCAGTAGATACCCAGAAGGCAAAATCCAGTTCTTCACCTGAAGTTGTTAAACCCAA GATCACTCATTCTCCTGATTCTGTGAAGTCTAAAGCTGCTTATGGGAACAGTCAAGCTGTTGGTGAGCGA AGGCTGGCAAATAAAATAGAGCATGAGTTATCCAGAGGTAGTTTCCATCCAGTTCCTACTCGAGGCAGTG CTTTGGAAACTACAAAGAGCCCTCTTATCATTGATAAAAACGAGCACTTCACAGTCTATAGGGATCCTGC TCTTATTGGATCAGAAACAGGAGCTAATCACATTTCACCTTTTTTGAGCCAGCATCCTTTTTCTCTTCAT TCCTCATCCCATAGAACCTGTTTAAACCCAGGTACCCATCACCCTGCCTTAACGCCAGGACCCCACTTAC TAGCTGGGTCAACTAGTCAAACTCCATTACCTACCATTAACACTCACCCTCTCACTAGTGGTCCACACCA TCCTGTCCATCACCCTCATTTGCTTCCCACTGTGTTACCTGGAGTGCCTACTGCCTCTTTACTCGGTGGC CACCCACGTCTAGAGAGTGCTCATGCAAGCAGCTTGAGCCATTTAGCACTAGCACACCAGCAGCAGCAAC AGTTGTTACAGCACCAGTCACCTCATCTTCTTGGACAAGCCCATCCTTCCGCTTCATATAATCAGCTTGG ACTTTATCCAATTATTTGGCAATATCCGAATGGAACACATGCATACTCAGGACTTGGTCTCCCTTCTTCT AAGTGGGTTCACCCAGAAAATGCAGTTAATGCTGAAGCTTCATTAAGGAGGAATTCTCCCAGTCCTTGGC TACATCAACCCACTCCTGTGACCTCAGCGGATGGTATTGGATTACTTAGTCACATTCCTGTCAGACCTTC CAGTGCAGAGCCTCATCGACCTCACAAGATTACAGTGCATTCCAGTCCCCCACTGACAAAGACATTAGCA GATCACCACAAAGAAGAATTGGAGAGGAAAGCTTTTATGGAACCATTACGGTCTAATGCATCCACATCAG TAAAAGGTGACCTGGATCTTAATAGGTCACAGGCAGGAAAAGATTGTCACTTGCATAGGCATTTTGTGGG TCCAAGGCCACCCCAGGAGACTGGAGAGAGACTGAACAAATACAAGGAGGAACATCGTCGGATTCTTCAG GAGAGTATTGATGTGGCTCCATTTACAACCAAAATCAAGGGTCATGAGGTTGAAAGAGAGAATTACTCCA GAGTAGTACCATCATCTTCTAGTCCTAAAAGCCATGCTATCAAACAAGATAAAGACGTAGACCGCTCCGT GTCAGAGATTTATAAGATGAAGCACTCAGTGCCTCAGAGTTTGCCCCAGAGTAACTACTTCACTACTTTA TCTAATAGTGTGGTCAATGAGCCACCAAGATCCTACCCATCCAAAGAAGTTTCCAACATTTACACTGAGA AACAAAATAATAACCTTTCTGCAACAGCCAATCCTCAAACTCATTCTTTTATATCATCTCTTTCGAAGCC TCCACCTTTGATTAAACACCAACCAGAAAGTGAAAGTTTAGTGGGCAAGATACCAGACCATCTTCCCCAT CAGAGTGCATCTCACTCAGTAACAACGTTCAGAAGTGATTGTCGGAGCCCTACCCATCTGACTGTTTCTT CTACTAATGCACTCCGCAGCATGCCTGCTCTGCACAGGGCACCAGTATTCCATCCACCAATCCACCACAG TTTGGAAAGAAAGGAAAGCAGCTACAGTAGCCTTTCCCCTCCAACACTGACACCGGTGATGCCAGTAAAT GCTGGTGGCAAAGTTCAAGAATCTCAGAAGCCTCCAACTCTAATTCCTGAGCCAAAAGACTCTCAGTCAA ATTTTAAGAATTCTTCTGATCAGAGTCTAACGGAAATGTGGAGATCTAATAACAACCTCAACAGAGAGAA AGCTGAGTGGCCCGTGGAAAAGAGTAGTGGAAAGTCCCAGGCTGCTGTGGCATCTGTCATTGTTCGTCCA CCTTCTAGTACAAAAGTTGATAGTGTGCCATCTGTACCATTAGCTTCCAAAGATCGAGTTTGTGAAAGAT CTTCATCTGGGGCAAATAAAACAGATTACCTCAAACCAGAAGCCGGAGAGACTGGAAGAATTATTCTACC AAATGTGAATTTAGAAAGTGCTCATGTGAAATCTGAAAAAAACTTTGAAGCTGTCTCCCAGGGCAACGTT CCTGTTTCAGTCATGTCTGCTGTAAATGTAGTCTCTACTACCAAAGCAGATGTGTTCACATCTGCTGCCA CTACCACCAGTGTCTCCAGCTTGAGTAGTGCAGAAACAAGTTATTCTTTATCAAATACCATTTCGGCCTC TACACCCTTCGAATGTACTTCTTCAAAAAGTGTTGTTAGTCAGGCAGTGGCGCAAGCAAAGGACTGCACA GTCAGCACCGCGGTTCCAGGCACTCTGGCCTGTAGTAAGACAGGAAGTGCTGTTCAGCCCGGCTCTGGCT TCTCAGGCACAACTGATTTTATTCATTTAAAAAAGCACAAGGCAGCGTTGGCTGCAGCTCAGTTTAAAAA TAGTAGTGTCAGTGAGGCTGAACTTAATACTGTGAGAAATCAGACAGTGGCAGCCTCTCTTCCCCTGGAT AGCACCATGACCTGCACTGCAAGTAACAAAGCAATCTCTGTAGGAAATGGGCCAGCAGCCCAGTCCAGTC AGCCCAACTACCACACTAAACTGAAGAAGGCCTGGCTCACCAGACATTCAGAAGAAGATAAAAATACTAA TAAAATGGAAAATTCAGGGAATTCTGTATCCGAAATTATTAAGCCATGTTCTGTCAACTTAATAGCCTCT ACATCTAATGATATAGAAAATCGTGCAGATGGCAGAGTCGCTGTAGATAAATATGGAAGAGATGAGAAAG TCAGCAGGAGAAAAGCCAAAAGAACTTACGAATCTGGCTCTGAAAGTGGAGACTCAGATGAGAGCGAAAG CAAGTCGGAGCAGAGGACTAAACGGCAGCCGAAGCCAACTTATAAAAAGAAGCAGAACGACCTGCAGAAG AGGAAAGGCGAGGTCGAAGAGGACTCGAAGCCTAATGGAGTTCTCAGCAGGAGCGCCAAGGACAAAAGTA AACTGAAGTTGCAGAACAGTAATAGCGCTGGCGTTCCTCGTTCCGTGCTGAAAGACTGGCGGAAGGTCAA GAAGCTGAAGCAAACTGGAGAATCATTTCTGCAGGATGACTCCTGCTGTGAGATAGGTCCCAACTTACAG AAGTGCCGAGAGTGCAGACTTATCCGGAGTAAGAAGGGGGAAGAATCAACTCACTCACCGGTGTTTTGTA GATTTTACTACTTTAGGAGATTGTCCTTTAGTAAGAATGGAGTAGTCAGAATAGATGGTTTCTCTTCTCC TGATCAATACGATGATGAAGCCATGAGTTTATGGACCCATGAAAATTATGAAGATGATGAAGTAGATGTT GAAACCTCTAAGTATATCTTGGACATAATAGGTGATAAGTTCTGTCAGTTAGTAACGTCTGAGAAAACAG CGTTGTCCTGGGTGAAAAAGGATGCCAAAATTGCATGGAAAAGGGCAGTTCGAGGTGTCCGGGAGATGTG TGATGCGTGCGAGGCCACACTGTTCAACGTCCACTGGGTTTGCCGCAAGTGTGGATTTGTGGCTTGCTTG GACTGTTACAAGGCCAAGGAAAGGAAGAGTTCTAGAGATAAAGAGCTGTATGCTTGGATGAAGTGTGTGA AAGGCCAGCCCCATGACCATAAGCATCTGATGCTGACTCAGATTATCCCTGGCTCTGTGTTGACAGACCT TCTTGATGCCATGCACATTCTTAGGGAAAAATATGGTATTAAATCCCATTGTCACTGTACAAACAGACAG AATTTACAAGGTGGAAATGTTCCTACAATGAATGGTGTGTCTCAAGTTCTACAGAATGTTCTTCACCACA GTAATAAGACTTCTGTGTCCCTGCCTGAGTCTCAGCAGCAGAACAGCCCTCAGAAATCTCAGACTAACGG CAACAGTAGCCCTGGAAGTGCAAGTACAGACAGCAGGTTGACACCTCCAGAGTCCCAGTCACCGCTGCAC TGGCTAGCTGATCTTGCTGAGCAGAAATCCAGAGAGGAAAAACAAGAAAACAAAGAATTTACTCTTGAAA GGGAAATCAAAGAAGATGGAGACCAAGATGCCTCTGATTCTCCAAATGGCAGCACATCACCTCCAGCATC CCAGAGCAACGAACAGGGTTCAACCCTACGTGATTTGCTGACCACAACAGCTGGCAAGCTTCGTGTGGGC TCTACAGATGCTGGCATTGCCTTTGCGCCAGTATATTCAATGGGAACCTCAAGTGGCAAAGGTGGCCGGA CTATGCCTAACATTCTTGATGACATCATTGCTTCAGTTGTTGAAAACAAAATTCCACCAAATAAAACCTC CAAGATCAATATAAAATCAGAACCTAATGAAGAACCCAAAGAAAGCAGTCTGCCTGCCACGGATGAAAGC AATAAGTCTTATCGTGACATACCACATTCTTGGATCTGTGACCAGCATATTTTATGGCTTAAAGATTACA AGAATAGTAATAACTGGAAGCTTTTCAAAGAGTGCTGGAAACAAGGACAGCCTGCAGTGGTTTCCGGTGT GCATAAGAAAATGAACATTAGCTTGTGGAAGGCAGAATCAATTAGTCTGGATTTTGGAGACCACCAAGCT GATCTCCTCAACTGCAAAGACAGCATTGTTTCCAATGCCAACGTTAAGGAGTTCTGGGATGGTTTTGAAG AAGTGTCAAAACGTCAAAAAAACAAAGGTGGAGAAACAGTAGTATTAAAGTTGAAAGACTGCCCATCTGG GGAGGACTTCAAGGCCATGATGCCAACAAGATATGAAGACTTTTTAAGATGTCTGCCATTACCAGAATAT TGCAACCCAGAAGGGAAGTTCAATTTGGCCTCCCATTTGCCAGGGTTCTTTGTACGCCCTGATCTAGGAC CCAGGCTGTGCAGTGCCTATGGTGTTGCTGCTGCTAAGGATCATGATATAGGAACCACAAATCTTCACAT TGAAGCTTCTGATGTTGTCAATGTCCTAGTCTATGTCGGCATAGCAAAAGGAAATGGCGTCCTCTCAAAA GCAGGAATACTTAAGAAATTTGAAGAAGAAGAATTAGATGATGTTTTAAGAAAAAGATTAAAGGATTCGA GTGAAATCCCTGGTGCTCTATGGCATATTTATGCTGGAAAAGATGTTGACAAGATAAGGGAATTTCTTCA AAAGATTTCAAAAGAACAAGGCCTTGAAGTTCTGCCAGAGCATGATCCAATCCGGGACCAAAGCTGGTAT GTGAATAGAAAGCTCCGCCAGAGACTGCTTGAGGAATATGGGGTGAGAGCCTGCACTCTGATTCAGTTCC TTGGTGATGCCATTGTTTTGCCAGCAGGAACACTTCACCAGGTACAAAATTTTCACAGCTGTGTTCAGGT GACCGAAGACTTTGTCTCTCCAGAACATCTTGTACAGTCGTTTCACTTAACACAGGAGCTGAGGCTTTTG AAGGAAGAAATCAATTACGATGATAAACTCCAGGTTAAGAATATCTTGTATCATGCAGTCAAGGAAATGG TGAGAGCTCTGAAGATGCATGAAGATGAAGTAGAGGATATGGAAGACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231944 representing NM_001242396 Red=Cloning site Green=Tags(s) MQGPYSLNGYRVRVYRQDSATQWFTGIITHHDLFTRTMIVMNDQVLEPQNVDPSMVQMTFLDDVVHSLLK GENIGITSRRRSRASQNISTVHGHYTRAQANSPRPAMNSQAAVPKQNTHQQQQQRSIRPNKRKGSDSSIP DEEKMKEDKYDCVSRGENPKGKNKHVVTKRRKPEEAEKRLSMKRLRTDNASDASESSDAESSSKRVTETS SSEPMPEYEPKNKVTSKVNGEEGQSQAAEEAGEETLIDTRPPWDQMQEDKNHNEGEKPKSTDSHLQDKMT LRSSEQATVADHNSNDSVLQECNVENQRTVELLPKDRLVSRTPTPKCVTDIKNDTHSERAAQENLNTFGL QTPENMDPNVSDSKHSNAKYLETAKQDCDQSWVSDVVKVDLTQSSVTNAPSGSDKRDTEKERNHYVSYMS SLSAVSVTEDQLHKRSPPPETIKAKLTTSVDTQKAKSSSSPEVVKPKITHSPDSVKSKAAYGNSQAVGER RLANKIEHELSRGSFHPVPTRGSALETTKSPLIIDKNEHFTVYRDPALIGSETGANHISPFLSQHPFSLH SSSHRTCLNPGTHHPALTPGPHLLAGSTSQTPLPTINTHPLTSGPHHPVHHPHLLPTVLPGVPTASLLGG HPRLESAHASSLSHLALAHQQQQQLLQHQSPHLLGQAHPSASYNQLGLYPIIWQYPNGTHAYSGLGLPSS KWVHPENAVNAEASLRRNSPSPWLHQPTPVTSADGIGLLSHIPVRPSSAEPHRPHKITVHSSPPLTKTLA DHHKEELERKAFMEPLRSNASTSVKGDLDLNRSQAGKDCHLHRHFVGPRPPQETGERLNKYKEEHRRILQ ESIDVAPFTTKIKGHEVERENYSRVVPSSSSPKSHAIKQDKDVDRSVSEIYKMKHSVPQSLPQSNYFTTL SNSVVNEPPRSYPSKEVSNIYTEKQNNNLSATANPQTHSFISSLSKPPPLIKHQPESESLVGKIPDHLPH QSASHSVTTFRSDCRSPTHLTVSSTNALRSMPALHRAPVFHPPIHHSLERKESSYSSLSPPTLTPVMPVN AGGKVQESQKPPTLIPEPKDSQSNFKNSSDQSLTEMWRSNNNLNREKAEWPVEKSSGKSQAAVASVIVRP PSSTKVDSVPSVPLASKDRVCERSSSGANKTDYLKPEAGETGRIILPNVNLESAHVKSEKNFEAVSQGNV PVSVMSAVNVVSTTKADVFTSAATTTSVSSLSSAETSYSLSNTISASTPFECTSSKSVVSQAVAQAKDCT VSTAVPGTLACSKTGSAVQPGSGFSGTTDFIHLKKHKAALAAAQFKNSSVSEAELNTVRNQTVAASLPLD STMTCTASNKAISVGNGPAAQSSQPNYHTKLKKAWLTRHSEEDKNTNKMENSGNSVSEIIKPCSVNLIAS TSNDIENRADGRVAVDKYGRDEKVSRRKAKRTYESGSESGDSDESESKSEQRTKRQPKPTYKKKQNDLQK RKGEVEEDSKPNGVLSRSAKDKSKLKLQNSNSAGVPRSVLKDWRKVKKLKQTGESFLQDDSCCEIGPNLQ KCRECRLIRSKKGEESTHSPVFCRFYYFRRLSFSKNGVVRIDGFSSPDQYDDEAMSLWTHENYEDDEVDV ETSKYILDIIGDKFCQLVTSEKTALSWVKKDAKIAWKRAVRGVREMCDACEATLFNVHWVCRKCGFVACL DCYKAKERKSSRDKELYAWMKCVKGQPHDHKHLMLTQIIPGSVLTDLLDAMHILREKYGIKSHCHCTNRQ NLQGGNVPTMNGVSQVLQNVLHHSNKTSVSLPESQQQNSPQKSQTNGNSSPGSASTDSRLTPPESQSPLH WLADLAEQKSREEKQENKEFTLEREIKEDGDQDASDSPNGSTSPPASQSNEQGSTLRDLLTTTAGKLRVG STDAGIAFAPVYSMGTSSGKGGRTMPNILDDIIASVVENKIPPNKTSKINIKSEPNEEPKESSLPATDES NKSYRDIPHSWICDQHILWLKDYKNSNNWKLFKECWKQGQPAVVSGVHKKMNISLWKAESISLDFGDHQA DLLNCKDSIVSNANVKEFWDGFEEVSKRQKNKGGETVVLKLKDCPSGEDFKAMMPTRYEDFLRCLPLPEY CNPEGKFNLASHLPGFFVRPDLGPRLCSAYGVAAAKDHDIGTTNLHIEASDVVNVLVYVGIAKGNGVLSK AGILKKFEEEELDDVLRKRLKDSSEIPGALWHIYAGKDVDKIREFLQKISKEQGLEVLPEHDPIRDQSWY VNRKLRQRLLEEYGVRACTLIQFLGDAIVLPAGTLHQVQNFHSCVQVTEDFVSPEHLVQSFHLTQELRLL KEEINYDDKLQVKNILYHAVKEMVRALKMHEDEVEDMEDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242396
ORF Size	7050 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242396.1, NP_001229325.1
RefSeq Size	8617 bp
RefSeq ORF	7053 bp
Locus ID	108829
UniProt ID	Q69ZK6
MW	261.1 kDa
Gene Summary	Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes (By similarity).[UniProtKB/Swiss-Prot Function]

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