Frem1 (NM_001198811) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC037594; crf11; D430009N09; D630008K06; eye; eyes2; heb; qbrick
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231925 representing NM_001198811 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCAGTGGTTCTTGCTGCTCCAGCTGCTTCTGCTGCACC TGGACCGGGTCAGTGCCACCTTCATCAGCATCAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT CCTGTCTGGAGATCACCTGAGGGTTGCAGTCCCTAAAGAAAAGGACGCCTGCAGACTGGAGGTGGTGATG AACGAGCCCGTAACCCAGAGGGTTGGGAAACTCTCTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG AAGTGAAGTATGTTCACAATGGATGCCCAATTCTGGATGAAGACTCTGTGAAGCTTAGACTTTACAGGTT TACTGAAACAGACACCTTCATGGAAACCTTTCTCTTGCGGGTTTATCTTGTGGAACCAGACTGTAACATC ATCCGTATGAGCAGTAATGTGTTGGAAGTGACTGAATTCTATGGCTTATCGCAAGCCATTGATAAGAACC TGCTCCAGTTTGATTATGATAGGACTGCCAGCCTGGATTGCACCATCAGACTAGACCCACTGAGGACTCA GCTGCCAGCTCACGGCAAGCTGGTCGTGGTGAATCGTAAGTCAGAGGGGCCTCGTGGAGATCAGCCACAC AGCTTCTTCTCTGAGACAGAGCTAGGGGCTGGACTGAAGTGTCCAGATGGAAGCTGTGCCCTGGAATTGA AGCAAGTGGCAAGTCTGAAAGTGAGCTGTGAGGAGTTTCTGCTGACGGGGTTTCACTACCAACACATGCA GCCCCCTTCACCCAACATTGATTACATCCCCATTCAGTTGGATCTCACCGACAGAAGGAGCAAAACCGTA TACAAGTCAGAGAGTGCGTGGTTGCCTGTCTATATCAGAGTTGGCATTCCTAACCAGGTCCCAAGGGCTG CATTTATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATACTGACCCCTCTGACAACCTCCGTTCTGGA CTGTGAGGAGGATGAGACTCCGAAACCCTTGTTGGTGTTCAACATCACTAAAGCCCCACTCCAGGGCTAT GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTCACCTGGAAGGATCTCAGTGACATGCAAG TTGCCTACCAGCCACCAAACAGCAGCCACCCTGAGAGGAGACATTACACGATGGAGCTGGAGGTGTACGA CTTCTTCTTTGAGAGGAGTGCCCCCATCACTGTCCACATCTCCATCAGAACAGCAGACACGAATGCACCA CGGGTGTCTTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAATTTC AGATCGTTGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGCCTGCAGCATGGACGGCT TACAGTACGAGAGGGGAAAGGATTTCTCTTCACCGTGACTGACCTTCAAGCTGGAGTTGTCCGCTATCAT CACGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCACCACAGCAGCCACC ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGCCCCCCATTCCTCATCACCAATGTCGTGATTGA ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCTATGTTGAGAGCTTCAGATATGGACTCCAGTGAT GACTACATCTTCTTTAATATCACAAAGTTTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAC TGATAGGGTATCCTGTCCCTGGCTTCCTGCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT TGGTGGAGAAATCTTTGAAGATTCCTTTGAATTCGTACTGTGGGACAGCCATGAGCCTCCAAATCTCTCA GTGCCACAGGTGGTGACAATTCATATCACTCCAGTAGATGACCAGCTTCCTAAAGAAGCTCCTGGCATTT CCCGGCATTTGGTTGTCAAGGAAACAGAGGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAT GGAATCCCGTGATGGGGAACTCATCTACACAGTAACACGCCCTCCATGTTTCTCCTTCAGCCACAGACAC TTGGATGCTGGGAAGCTATTCATGGTGGACAGCATACCAAAGCTAACCAAAAATCCGACAGCCCCAGGGC TAAGTTCATTTACTCAGCATGCCGTGAACCATATGAAAGTGGCCTACATGCCACCCATGCAAGATATAGG CCCCTCCCCTCGACATGTGCAGTTCACAGTGTCTGTCAGTAACCAGCATGGAGGTGCTTTGCATGGAATC TGCTTCAACATCACAGTTCTCCCAGTGGACAACCAGGTTCCCGAGGTATTCACCAACACCCTGAGAGTGG TCGAGGGCGGTCAGTGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCGCTAGACAG CATCAGCCTCTCTCTTAAGGAGAGGCCTCTGCATGGCGGGGTAGAGCTGGACGGATTTCCTCTAAACCCA AGAGGCACATTTTCTTGGCGAGACCTCAATACCTTAAAAGTTTGGTATCAACATGATGGATCTGAGGTTC TTCAGGATGAGATATTTTTGGAGGTCACAGATGGCACAAATTCAGCAGCATTTGTTCTCCACATTGAGGT GTTCCCTGTGAATGATGAGCCACCCATTCTGAAGGCTGACCTCATCCCCATGATGCACTGCTCTGAGGGT GGAGAGGTGACCATCACCCCTGAATACATTTCCGCTACTGATGCAGACAGCGATGACTTAGAACTGCTGT TTCTGATTGCCCGAGAGCCTCAGCATGGAGTGGTGAGAAAGGCTGGACTTCACGTGGACAGGTTCTCTCA GGGAGATGTCATCTCAGGGGCTGTGACATACAAACATACAGGTGGCGAGATTGGCCTTGAACCTTGTTCT GACACTGTTGTATTGGTGGTTTCGGATGGTGAAGCTGACCCTTTGATGAACGGCTGTTGCTACGATGGAC CTGATTCATCTGTTCCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACAGTACATCCAGTCGA CAACCAGCCACCTTCAATTATAATAGGTAGGATGCTCACAGTGGATGAAGGCTTCTCAGCAGCCCTTACT ACTCATCACTTGACTGTTGTTGATTGGGACACTGCCCCGGATGACTTAAAATTCATGTTGGCTTCTCAGC CCCAGTTTGGCTACTTAGAAAACGCACTGCCTTCTGCAGGCTTTGAGAAAAGTAACATCGGCATAAGGAT AGCCTCATTCCAGTGGACAGACATGAAGGCTTCACACATTAACTATGTGCAGTCCAGGCACCTGAGAGTG GAACCAACTGCTGACCAGTTCACAGTATATGCCACAGACGGGAAGCATCGCTCCTTGGAGACAACATTTC ACGTTATCATCAACCCCACAAACGATGAAGCTCCTGACCTTGCAGTACAGAACATCACTGTGTATGAGGG GCACATGGTAGAACTGGATTCTTCCATCATCAGTGCTACTGACAGGGATATTCCCAAGGACCCCTTGCTC TTCAGCATCGCTCTCAAGCCCCAGCACGGCCTGCTCGTCGATGCTGCCATCAGCAAGGACTCCCATCAGA TCAAGCAGCTGCAACACGAAATCCACAGCTTCTCCGTGGACCTTCTCAAGAATGGAATGAAGCTGGTCTA TGCCCACGACGACTCAGAGAGCTCTGCTGATAACTTCGTGATCCAGTTGTCAGACGGGAAACATAAAATC CTTAAAACCATCTCAGTAAACATTACCCCAGTGAATGACGAGACACCAACGCTAAGCAAGAAGGCTGAAA TCTCAATGGCTGTGGGCGATACTCGCGTCCTGTCTAGTGCTGTTCTTTCAGCCACAGATAAAGACTCGCC CAGAGAGAAGATTCACTATGTATTTGAAAGACTTCCCCAAAATGGACAACTTCAACTTAAGATAGGGAGG GACTGGGTCCCTCTCTCCACTGGCATGCAGTGCACCCAGGAGGATGTGGACCTGAACTTGCTAAGATACA CGCATGCTGGTAAAACGGACTCCCAGGATGGAGACAGCTTCACCTTCTACCTCTGGGATGAGGACAACAG GTCACCTGCATTTGACTGTCACATCATCATTGAAGACATGGGCAAAGGTGATATTGTCATTCATGCAAAA CCACTCGTGGTAGTCAAAGGTGACAGAGGTCTCTTGACGACTGCCACCCTTCTGGCTGTGGATGGAGCAG ACAAGCCTGAGGAACTGCTCTACCTCATCACCTCCCCACCACGGCATGGCCAAGTGGAGTACGTCCACTC TCCTGGAGTCCCCATTGCCAGCTTCAGCCAAATGGACATAGCAGGACAGACAGTGTGCTACATACACAAG AGCAGGACAGCTGTCCCCACTGACAGCTTCAGATTTACCATCAGCAATGGACTGCAGACCCAGCGTGGGG TGTTTGAAATCACACTGCAGACTGTGGACAGCGCCTTGCCTGTGCTGACCAAGAACAAAAGGCTGAGGCT AGCCGAAGGGGCCATGGGCCTCCTGTCCGCTGATCACCTTCAGCTGACTGACCCCGACACACCTCCAGAG AACCTGACCTTCTTTCTGGCTCAGCTCCCACGCCACGGGTACCTCTTCCTGAGAGGGAAAGCACTCCAAC ACAACTTCACCCAGCGAGATGTGGACAGCGGGGGCGTGGCCTACCAGCACTCAGGAGGCGGCGCCCGGGA GGACTATTTTACTTTTCTAGCCACAGACAGGAAGAACCAAGGCTTTGTTGTGGATGGGAAAGTTCAGAAA GAGCCTGTTCGCTTCACAATCCAGGTGGATCAGCTGGACAAGGCAGCGCCCCGTATCACACACTTGCACT CCCCTACTCAAGTGGGGCTCTTGAAAAATGGCTGCTATGGGATTTACATCACTTCCCGTGTGCTGAAGGC ATCAGACCCTGACACAGAGGATGACCAGATCATCTTTAAGATTTTACGAGGCCCATTGTACGGACGTCTG GAGAACACAACAACAGGTGAATTCATCCACGAGCGATTTAGCCAGAAGGACTTAAGCCATAAGACCATCC TTTACATCATAAACCCTTCTTTGCAAGTGACTTCCGATATCCTGGAATTTCAGGCCATGGACCCCACAGG GAACACTGCCACTCCCCAGAGCTTGGACTTGAGATGGTCGTATATTGAATGGGCCCAGACTGCATATGAG GTTTGTGAGAATGTGGGCTTATTGCCCTTGGAAGTTACCAGAAGGGGATATCCCATGGACTCAGCCTTTG TGGGTGTAGAGGTTAACCAAGTGTCAGCTACAGTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCT TCAATTTGACCCAGGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAAGAT GATGAGGTCTTTGAAGTAATTCTGAACTCCCCTGTGAATGCGGTTCTTGGCACGCAGACAAAAGCGGCGG TGAAAATTTTGGACTCAAAAGGAGGACGGTGCCATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCAC GTGGGGGAAGGGCCCTTGGCATCCGCTGCCCTCAGGCTCATCCTCGCTCACCACTTCAGGTTCTCCTCTT CTGGAAAGGCCTCCTCCATCTTTCACCAGTGGAGATGCTCTTCAGGGTTTTGGTCTCACCGACCTCACTC AAAGGAAGACAATGACCCAGGGGAATGGCAAATCAGTTCTCCCATCCTCTGTTTGTAGAAATGGGACAGA CACCATCTACAATTACCATGGTATAGTTTCCTTGAAACTAGAGGGCGACAGATTCTCAGCTCACAAACGG AAGGCCAAAATATCCATTGTTAGTCAGCCACAGAGGACAATCAAGGTGGCAGAGCTGCCTCTAGCTGATA AGGTGGAATCCACAACTGATTTGCACTTTCTCAGACAGGGTCTGCGGCCCTTGTTTCCAAAGAATTGTTC TGTGGACTTAAAGGGACTCTTTCATTTTGAAGAAAGCACCCACAGATTGTATCAATGTGATGGGATCTCC TGGAAAGCCTGGAGCCCCCAAACCAAGGGGCTGGAGGACAGATCCTGCCCAGGTGGATGGCTCCTTCATT CTGGCTACTGTCACATCTTGGTCACAAGACAGAAAGGCACCTGGACCACAGCTACCCGAGCTTGCAGAGA ACAGCACCAGGGCGACCTTGTGACCGTACTCTCCAGAAGGCATATGCAGTGGCTATGGGCGATGAGTGGA AGAAAACCCTTCTGGATAGGCTTGAAGAACCAACCGAGGACTGGCCACTGGGAGTGGATCGGAGGTGAAC CTGTTGCTTTTACCAACTGGAGGAGAGGGGCCCCTCTGCACCCAAAACCTGGAAAGAACTGTGCTTTGGT TCAGAAAAGAGGACAATGGCAGACAAAGAACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGG AAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231925 representing NM_001198811 Red=Cloning site Green=Tags(s) MHSPGCTGPKAQWFLLLQLLLLHLDRVSATFISINRGLRVMKGSSAFLSGDHLRVAVPKEKDACRLEVVM NEPVTQRVGKLSPQVFDCHFLPNEVKYVHNGCPILDEDSVKLRLYRFTETDTFMETFLLRVYLVEPDCNI IRMSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRLDPLRTQLPAHGKLVVVNRKSEGPRGDQPH SFFSETELGAGLKCPDGSCALELKQVASLKVSCEEFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTV YKSESAWLPVYIRVGIPNQVPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITKAPLQGY VTHLLDHTRPISSFTWKDLSDMQVAYQPPNSSHPERRHYTMELEVYDFFFERSAPITVHISIRTADTNAP RVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVTDLQAGVVRYH HDDSDTTKDFVAFRIFDGHHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSD DYIFFNITKFPQAGEIMKKPGPRLIGYPVPGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLS VPQVVTIHITPVDDQLPKEAPGISRHLVVKETEVAYITKKHLHFLDMESRDGELIYTVTRPPCFSFSHRH LDAGKLFMVDSIPKLTKNPTAPGLSSFTQHAVNHMKVAYMPPMQDIGPSPRHVQFTVSVSNQHGGALHGI CFNITVLPVDNQVPEVFTNTLRVVEGGQCTISTEHILVSDVDTPLDSISLSLKERPLHGGVELDGFPLNP RGTFSWRDLNTLKVWYQHDGSEVLQDEIFLEVTDGTNSAAFVLHIEVFPVNDEPPILKADLIPMMHCSEG GEVTITPEYISATDADSDDLELLFLIAREPQHGVVRKAGLHVDRFSQGDVISGAVTYKHTGGEIGLEPCS DTVVLVVSDGEADPLMNGCCYDGPDSSVPLHKSFPTYQLNITVHPVDNQPPSIIIGRMLTVDEGFSAALT THHLTVVDWDTAPDDLKFMLASQPQFGYLENALPSAGFEKSNIGIRIASFQWTDMKASHINYVQSRHLRV EPTADQFTVYATDGKHRSLETTFHVIINPTNDEAPDLAVQNITVYEGHMVELDSSIISATDRDIPKDPLL FSIALKPQHGLLVDAAISKDSHQIKQLQHEIHSFSVDLLKNGMKLVYAHDDSESSADNFVIQLSDGKHKI LKTISVNITPVNDETPTLSKKAEISMAVGDTRVLSSAVLSATDKDSPREKIHYVFERLPQNGQLQLKIGR DWVPLSTGMQCTQEDVDLNLLRYTHAGKTDSQDGDSFTFYLWDEDNRSPAFDCHIIIEDMGKGDIVIHAK PLVVVKGDRGLLTTATLLAVDGADKPEELLYLITSPPRHGQVEYVHSPGVPIASFSQMDIAGQTVCYIHK SRTAVPTDSFRFTISNGLQTQRGVFEITLQTVDSALPVLTKNKRLRLAEGAMGLLSADHLQLTDPDTPPE NLTFFLAQLPRHGYLFLRGKALQHNFTQRDVDSGGVAYQHSGGGAREDYFTFLATDRKNQGFVVDGKVQK EPVRFTIQVDQLDKAAPRITHLHSPTQVGLLKNGCYGIYITSRVLKASDPDTEDDQIIFKILRGPLYGRL ENTTTGEFIHERFSQKDLSHKTILYIINPSLQVTSDILEFQAMDPTGNTATPQSLDLRWSYIEWAQTAYE VCENVGLLPLEVTRRGYPMDSAFVGVEVNQVSATVGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEED DEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRCHPSNSFNQSKHSTWGKGPWHPLPSGSSSLTTSGSPL LERPPPSFTSGDALQGFGLTDLTQRKTMTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKR KAKISIVSQPQRTIKVAELPLADKVESTTDLHFLRQGLRPLFPKNCSVDLKGLFHFEESTHRLYQCDGIS WKAWSPQTKGLEDRSCPGGWLLHSGYCHILVTRQKGTWTTATRACREQHQGDLVTVLSRRHMQWLWAMSG RKPFWIGLKNQPRTGHWEWIGGEPVAFTNWRRGAPLHPKPGKNCALVQKRGQWQTKNCSKGKAHNFVCSR KL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198811
ORF Size	6516 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198811.2
RefSeq Size	9524 bp
RefSeq ORF	6519 bp
Locus ID	329872
UniProt ID	Q684R7
MW	243 kDa
Gene Summary	Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development.[UniProtKB/Swiss-Prot Function]

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