Ralgapa1 (NM_001286263) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310003F20Rik; 4930400K19Rik; AI563624; Garnl1; GRIPE; mKIAA0884; Tulip1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231912 representing NM_001286263 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGACCTTAAACAGTTTTT TGACCAACATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG GATAACTGCAGCAAAGAACAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTCTCAGCACCACAATCCG AATATGGACCTCGGACTTTAGATAATCTTATTAATCCTCCACTCAGCCTTCAAGAAACTCAAGTAACTAT AGAGGAAGTCACTCCTCTTGTTCCGCCACAGTCAGGAGATAAAGGACAAGAAGACCTCACAAGCTATTTT CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACATTTG CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGATCAGACCAAAGCCTCATTATGTGATG ATAAAGAAGGATGCTGAAACCAATGAAACAATATATTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTTA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATTCAGGACCTAATATTCCTGGGAT GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTTTCCAGAGAAGAAAGCAAA AATGATACTGTTGATAAAGTAGACAAGTCTGCAGAGCCTGAGCAGTCTCATTCCAACACAAGCACTCTCA CGGAGCGAGAGCCTAGCTCTTCCAGTCTCTGCAGCATCGACGAAGAACATCTCACAGACATCGAAATTGT TCGCAGAGTGTTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTGAAAGTGTATCAAGAATGGATTCAACAAGAGG AAAAGCCTTTGTTTATGCAAGAGCCTGAGGACACTGCGATCACTTGTTCAGATATACCCTGCAGCGAAAC TGTTGCAGACCATGACAGTGCAATAGAAGATGGAGAGAAAAGAGAAGAGGAAAATGGGACCAGTACTTCT GAACATGTTCGCAATTCTAGCTGGACAAAAAATGGTTCCTACCAAGAAGCTTTTCATGTCTGTGAAGAAG CCACAGAACAGAATATTCAAGCTGGCACTCAGGCCGTTTTGCAGGTGTTTATTATAAACTCATCGAACAT ATTTCTTCTTGAACCTGCAAATGAAATAAAGAATCTTCTGGATGAGCATACAGATATGTGTAAACGCATT CTTAATATTTATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTGCTCG TGCTGCTCAGAGTCACGGAGTCCGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA AAGCATGACCCTGGCGGGTCGACTTGCAGGACCACTTTTTCAGACTCTCATTGTTGCCTGGATCAAAGCA AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCGGTATTGTCATCATTGACCTATT GGGAAGAATTGGCCACTGAGTGGTCACTGACCATGGAGACACTGACTAAAGTTTTGGCTAGAAATTTATA TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA GGAGTTGGACACGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC CTGGCCAGGCACCTATGAGACAGAGAAGTGCAACGACCACTGGCTCCCCAGGAACTGAGAAGGCCAGGAG TATAGTACGGCAAAAAACTGTTGCCATGAGAAGCCGATCCATTGGTGAATGTGCTCTGCCATCGGCCTAT ATTCGCAGTGCTAAAAGTGCTCCTGTTCTGATCCATACTTCCAAACCCTTCTTGCCTGATATTGTTCTCA CTCCCCTTTCTGATGAGCTTTCAGATATTGATGATGCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACA TTTTTCACAGAGTGAAGACACTGGAAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGA AGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGA GCCCCAAACTGCCTCCTCTTAATAGTGAGACTGGTGGCAACAGCGCCAATGTTCCTGACCTGATGGATGA GTTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGGAGTAGTCCAGGCAGC CTTGAAATCCCGAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGGCCTGTTGATGACCCTG GTGTACCCTCCGAATGGACGTCTCCAGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTC GGATTCCTTCAGTGCCTTCCAGTGTGAGGGACGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGG ATCCCATCTTCTGCTGATGTGGATTTAGGTTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCCA GCCTAACTACGCTTCATTTAGATTCAGAAACAAGCAGCCTTAATCAGCAAGCCTTCTCTGCAGAAGTTGC AACCGTTACTGGTTCAGAAAGTGCTTCCCCCGTTCATTCAGCTCTGGGATCCAGGTCGCAGACCCCCTCC CCATCTACACTGAGCAGAGCTCACATAGAACAGAAGGACCTACAGCTCGATGAGAAGCTCCACCACTCTG TTCTTCAGACGCCGGATGACCTAGAAATCAGTGAATTTCCATCTGAATGTTGTAGCGTGATGGCAGGAGG TACTCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGCAGAATGCTGGGCATTTTGGGAGAT GTCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATC TAGCCAAGATCAGAGATAACCTTGGCATTTCAGCTGACAACCTGACTTCCCCTTCTCCACCCGTCCTGAT TCCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGT AAATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCTCCAAATAGAGATT TTTTAACACATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATTGTCAATACGAT CATCAAGCACTGCTCACCTCAGTTTTTTTCACTCGGTTTGCCTGGGGCCACGATGCTTATTATGGATTTT ATTATAGCAGCTGGGAGAGTGGCTTCTTCGGCTTTTCTTAATGCACCAAGAGTGGAAGCACAAGTTCTTC TGGGATCTTTAGTTTGCTTTCCCAACTTATATTGTGAATTGCCTGCTCTCCATCCCAACATTCCTGATAT TGCTGTGTCTCAATTTACAGATGTTAAGGAACTTATAATTAAAACTGTATTAAGCTCGGCAAGAGATGAG CCCTCTGGTCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATTTGGATTTGTGAAGAATTAGTCCACG AATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAAC AGTTGCCCACGTCGCCTGTAACATGCTTCACATGCTGGTTCACTATGTCCCTAGACTTCAGATTCACCAG CCGCAGTCTCCCCTGAAAATTATTCAGATCCTAATAGCAACTATCACCCACCTTTTGCCAAGTACAGAAG CTTCATCATATGAAATGGACAAGAGGTTGGTTGTCTCATTACTTCTTTGCCTTCTGGACTGGATCATGGC CTTACCTTTAAAGACACTGCTCCAACCAGTCCATGCTACAGGAGCAGAAAATGATAAGACCGAAAAGTCT GTTCTCAACTGCATTTATAAGGTATTACATGGGTGTGTCTATGGAGCGCAAAGTTTTAGCAATCCCAAGT ACTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGATCCGTTTATGCATTTGGAAAGTCTGAA AGAACCTGAACCTTTACACTCTCCAGATTCAGAGCGGTCTTCTAAACTGCAGCCTGTGACAGAAGTGAAA ACTCAGATGCAACAAGGATTAATCTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACTTGG GCCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCATGACAATCATTA CAGTGAAAGTACTGAACTTTCTCCTGAGCTCTTTGAAAGTCCAAACATCCAGTTCTTTGTGCTGAATAAT ACAACCTTAGTGTCCTGTATCCAGATAAGATCGGAAGAGAGTATGCCTGGAGGAGGTCTAGCTGCTGGCC TTGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGC CATACTGTATGGACCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTGAC CAAGAGAAACCGGAAGAGCCTCCTACATCTAACGAATGCTTAGAAGACATCGCAGTAAAAGATGGGCTTT CTCTCCAGCTTAGAAGATTTAGAGAAACTGTACCAACTTGGTCTACGATACGAGAGGAAGAAGATGTTCT TGACGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAGTGTTTACAGAGAACGGGAATCTCCCTCAAT GTTCCTGCTCCACAGCCTCTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGC AATACACGGAAGAAAAAGAGTTTGTAGAGAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGA TGAGCCAACACCTCAGAAACCTCAGTCAGCTTTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATG AATTCCTGGGACAAACGGAGGAGCTTTCATCTCTTGAAGAAAAATGAAAAGCTGCTCAGAGAACTTAGGA ACTTGGATTCAAGGCAATGCCGAGAGACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGA TAAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTAGCTGGCCTTGGTTGG GAGGTAAACCTTACAAACCACTGTGGCTTCATGGGAGGCCTTCAGAAGAACAGAAGCACTGGACTGACCA CTCCCTATTTTGCTACCTCGACAGTAGAAGTAATATTTCATGTATCTACACGAATGCCTTCTGATTCTGA TGACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTGCACATTGTTTGGTCAGAACATACT AGAGACTACAGGAGAGGAATTATTCCTACTGAGTTTGGTGATGTCCTCATTGTAATATACCCAATGAAAA ACCACATGTTCAGTATCCAGATCATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCACTTTTTGATGGTGC TATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAA TCACTGATTCCATTGTATCAAAATTTCTATGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACC ATTTAGAGCCAACAACATTTGAAGACTTTGCAGCACAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCC TGCTGACGCAGATCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231912 representing NM_001286263 Red=Cloning site Green=Tags(s) MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLK QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ DNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQEDLTSYF LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRPKPHYVM IKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESK NDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL LPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTS EHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRI LNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKA NLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGK GVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAY IRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALHEEQPLPR SSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGNSANVPDLMDEFIAERLRSGNASTMTRRGSSPGS LEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCEGRKFDNFGFGTDIG IPSSADVDLGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPS PSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGD VNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQG KLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDF IIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDE PSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIHQ PQSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKS VLNCIYKVLHGCVYGAQSFSNPKYFPISLSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVK TQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNN TTLVSCIQIRSEESMPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSD QEKPEEPPTSNECLEDIAVKDGLSLQLRRFRETVPTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLN VPAPQPLCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQSAFYYCRLLLSILGM NSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGW EVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHT RDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALK SLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFPADADH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286263
ORF Size	6246 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286263.1, NP_001273192.1
RefSeq Size	8421 bp
RefSeq ORF	6249 bp
Locus ID	56784
UniProt ID	Q6GYP7
MW	234.9 kDa
Gene Summary	Catalytic subunit of the heterodimeric RalGAP1 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB (By similarity). May interact with the HLH region of TCF3/isoform E12.[UniProtKB/Swiss-Prot Function]

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