Myo18a (NM_001291212) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAJN; MyoPDZ; MysPDZ; SP-R210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231910 representing NM_001291212 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACCTCATGAAGAAAGATAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAAAAAAAGGAGA AGAAGGAGAGGATGTCTGCGGCAGAACTGCGGAGCCTGGAAGAGATGAGCATGCGCCGTGGCTTTTTCAA CTTGAACCGGTCCTCCAAGCGGGAATCTAAGACGCGCCTGGAAATCTCCAACCCCATCCCTATCAAGGTG GCCAGTGGCTCTGACCTGCACCTGACGGACATTGACTCTGACAGCAATCGGGGTAGCATCATCCTGGACT CAGGACACCTAAGCACAGCCAGCTCCAGTGATGACCTCAAGGGTGAAGAAGGCAGCTTCCGGGGCTCTGT GCTGCAGCGGGCAGCCAAGTTCGGTTCACTGGCCAAGCAGAACTCTCAGATGATTGTCAAACGTTTTTCC TTCTCTCAACGGAGCCGGGATGAGAGTGCCTCGGAGACCTCAACCCCCTCAGAGCACTCTGCAGCCCCAT CACCTCAGGTAGAGGTGCGGACGCTTGAGGGACAGCTAATGCAGCATCCTGGGCTAGGCATTCCTCGACC AGGACCACGGTCTCGAGTCCCTGAGCTGGTGACTAAAAGATTCCCAGCTGATCTACGTCTTCCCGCTCTG GTGCCACCACCACCTCCTGCTCTCCGGGAGCTGGAGTTACAACGAAGACCCACAGGAGACTTTGGCTTCT CACTGCGGCGCACAACCATGCTGGATCGGGCCCCTGAGGGTCAGGCTTATCGACGGGTGGTTCACTTTGC TGAGCCAGGGGCAGGCACCAAGGACCTGGCCTTGGGGCTGGTACCAGGAGATCGACTGGTGGAGATTAAT GGACAGAATGTGGAGAATAAATCCAGGGATGAAATTGTGGAGATGATCCGGCAGTCTGGGGACAGTGTGC GGCTCAAGGTACAGCCCATCCCAGAGCTTAGCGAGCTGAGCCGGAGCTGGCTTCGGACTGGCGAGGGACA CCGCAGGGAGCCGGCTGATCTGGACCCCGAGGCCGCCTCTCCTGCCTACAGCCAGGCCAAGACGGAAGAG CAGATTGCCGCTGAAGAGGCCTGGTATGAGACGGAGAAGGTGTGGCTGGTCCATAGAGATGGATTCTCCT TAGCCAGCCAGCTCAAGTCTGAGGAGCTCAGCCTACCCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGA CGGAGCCATCCTGGATGTGGATGAAGATGATATAGAGAAGGCTAATGCTCCTTCATGTGACCGTCTAGAA GATCTGGCCTCACTGGTATACCTCAACGAGTCTAGCGTCCTGCATACACTGCGCCAGCGCTATGGGGCTA GCCTGCTACACACCTATGCTGGCCCCAGCCTGCTTGTCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTC AGAGAAGGTGATGCACATGTTCAAGGGGTGTCGGCGGGAGGACATGGCACCCCACATCTACGCTGTAGCC CAGACTGCATATAGGGCGATGTTGATGAGTCGTCAGGACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCA GTGGCAAGACTACCAGCTTTCAGCATCTGGTGCAGTATCTGGCTACCATCGCGGGTACCAGTGGGACCAA GGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGTCCACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATC ATGAATGGCAGTGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTT CGGCCTCCATCCAGACAATGCTCCTGGAGAAGCTGCGTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTT CAATGTCTTCTACTACTTGCTGGCCTGTGGGGATGCCACCCTCAGGACAGAGCTCCACCTGAACCACTTG GCAGAGAACAATGTGTTTGGAATTGTGCCACTGTCCAAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGT TCAGTAAGCTCCAGGCTGCCATGAAGGTGCTGGCCATCTCCCCCGAGGAACAGAAGACCTGCTGGCTCAT CCTGGCTTCCATCTACCACCTGGGGGCTGCTGGAGCTACCAAAGAAGCTGCTGAAGCTGGACGAAAGCAG TTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATACCTGCTGGGCTGCAGCTTGGAAGAGCTGTCCTCGG CTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGCAACGGTCCACCTCCTTCCGTCAGGGCCCTGAGGA GAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCCTCTAC AGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAACAGGGCTCTCAAGTCCAGCCAGCACTCACTCTGTT CCATGATGATCGTGGACACACCAGGCTTCCAGAACCCTGAATGGGGCGGCTCGGCCCGTGGAGCCTCCTT CGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCTGCAGAGGCTGTTCCACGAGCGCACCTTCCTCCAG GAGTTGGAGAGATACAAGGAGGATAACATAGAACTGGCGTTTGATGACTTGGAGCCAGTTGCAGATGACT CCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTCGCTCACTGGCCCATGCCGATGAGGCAAGAGGGCT ACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGGTGCGACAGAGGATGCCCTCCTTGATCGTCTTTTC TCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGCCAGAGTCCTCTTCTGCGAAGTAGCAAACCTCGGC ACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGGTGGAGTACAACGTGGCTGGCTGGCTGAATTACAC CAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCTTCTGCAGGACTCCCAGAAAAAGATCATCAGTAAC CTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTCTCAGGCTCGATTGCAGGCCTGGAAGGTGGCTCCC AGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAACCTTCACCACCGGCATGGCCGCTGTCAAGAAGAA GTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGCCCTCATTGATACCATCAAGAGGTCCAAGATGCAT TTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGGCCTGGGGAGCCCCGGTCTGCCTCTTCCCGCAGAG TCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAGACCCCTGTGAGGCTGGGCTGCTACAACTGGATGT GTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCTGCTTGATGCGATGCGCATGTACCGCCAAGGTTAC CCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGCTTTGATGTCCTGGCTCCACACCTGACCAAGAAAC ATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGGCTGTGGAAGAGCTGCTGGAGTCCCTGGACCTGGA AAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTTCTTCCGGGCAGGCACGTTGGCACGGTTGGAAGAA CAACGAGACGAGCAAACCAGCAGGCACCTGACCCTGTTCCAGGCAGCCTGCCGGGGCTACCTTGCCCGCC AGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAAGAAGAACAA GGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCACCACCGTGCGGCCCCTCATCCAAGTTCAGCTGTCA GAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAGCAGCTGCGCAGCAAGCTTGAGAAGGTGGAGAAGG AGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAGAGACCCGGATCTCAGAACTGACATCGGAGCTGAC TGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGGCTCCGGACC GAGAAGGAGATGAAAGAGCTACAGACCCAGTATGACGCACTGAAGAAGCAGATGGAGGTGATGGAGATGG AAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGATCAACGGGGAGGTGGATGATGATGATGCAGGGGG AGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTCCAGCAGGAG CTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCTGCAAG CAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGCTCAAGAAGAAGTGCCAAAGGCTCACAGCTGAGCT GCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGAAACCACGAGCTGGAGAAGAAGCAGAGGAGG TTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACGCAGCGGGAGAAGCTGCAGAGGGAGAAACTCCAGC GGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCTTGAAGCAGCAAATGGAGGAAAAAGACTTGGACAT TGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGCTGAGCTTCAGGACATTTCTTCTCAAGAGTCTAAG GATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGGACCAGGAAG AGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATGGAGAT GGAGAGAATGAGACAGACCCATTCCAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAAGAGGCCCGGCAG TCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAGCTCGAGGAGGAGTATGAAGACAAGCAGAAGGCGC TGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCGGGACTTTGA ATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCCCAGATCATGCTG GACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGGAAGAGTCGG AATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAGCGATGGAGGTGGAGATGGAAGACCTGCACCTGCA GATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAACTGAGTCGACTTCAGCGTGAGAAAAAC GAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGACATGAATGAGCTGATGAAGAAGCACAAGGCAGCTG TGGCCCAGGCCTCCCGGGACATGGCACAGATGAACGATCTCCAGGCTCAGATTGAAGAGTCCAACAAGGA GAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTG GACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGTTCGAAAAGA CCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGCTCAAAGAAACCATGGAGAAGCTGACTGAGGAACG GGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAGCTTCGTGAC ACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAAGCCGAGGCTAGCCGCAAGAAGCATGAACTGGAGA TGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCCTGCAAGCCGACCTAAAGCTGGCGTTCAAACGCAT TGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGAAAGTGACGAGAACGAGGACCTCATCAACAGTGAG GGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGGGTCGACGGGGTCAAGTCCTGGTTGTCAAAGAACA AGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCTTGAAGAGTTCCAGCCCAACCAGCCACTGGAAGCC ACTCGCCCCTGACCCATCGGATGATGAGCATGATCCTGTGGACAGCATCTCCAGACCCCGGTTCTCCCAC AGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACAGAGACCAGTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231910 representing NM_001291212 Red=Cloning site Green=Tags(s) MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSMRRGFFNLNRSSKRESKTRLEISNPIPIKV ASGSDLHLTDIDSDSNRGSIILDSGHLSTASSSDDLKGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS FSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLMQHPGLGIPRPGPRSRVPELVTKRFPADLRLPAL VPPPPPALRELELQRRPTGDFGFSLRRTTMLDRAPEGQAYRRVVHFAEPGAGTKDLALGLVPGDRLVEIN GQNVENKSRDEIVEMIRQSGDSVRLKVQPIPELSELSRSWLRTGEGHRREPADLDPEAASPAYSQAKTEE QIAAEEAWYETEKVWLVHRDGFSLASQLKSEELSLPEGKARVKLDHDGAILDVDEDDIEKANAPSCDRLE DLASLVYLNESSVLHTLRQRYGASLLHTYAGPSLLVLSTRGAPAVYSEKVMHMFKGCRREDMAPHIYAVA QTAYRAMLMSRQDQSIVLLGSSGSGKTTSFQHLVQYLATIAGTSGTKVFSVEKWQALSTLLEAFGNSPTI MNGSATRFSQILSLDFDQAGQVASASIQTMLLEKLRVARRPASEATFNVFYYLLACGDATLRTELHLNHL AENNVFGIVPLSKPEEKQKAAQQFSKLQAAMKVLAISPEEQKTCWLILASIYHLGAAGATKEAAEAGRKQ FARHEWAQKAAYLLGCSLEELSSAIFKHQLKGGTLQRSTSFRQGPEESGLGEGTKLSALECLEGMASGLY SELFTLLISLVNRALKSSQHSLCSMMIVDTPGFQNPEWGGSARGASFEELCHNYAQDRLQRLFHERTFLQ ELERYKEDNIELAFDDLEPVADDSVAAVDQASHLVRSLAHADEARGLLWLLEEEALVPGATEDALLDRLF SYYGPQEGDKKGQSPLLRSSKPRHFLLGHSHGTNWVEYNVAGWLNYTKQNPATQNAPRLLQDSQKKIISN LFLGRAGSATVLSGSIAGLEGGSQLALRRATSMRKTFTTGMAAVKKKSLCIQIKLQVDALIDTIKRSKMH FVHCFLPVAEGWPGEPRSASSRRVSSSSELDLPPGDPCEAGLLQLDVSLLRAQLRGSRLLDAMRMYRQGY PDHMVFSEFRRRFDVLAPHLTKKHGRNYIVVDEKRAVEELLESLDLEKSSCCLGLSRVFFRAGTLARLEE QRDEQTSRHLTLFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLFTTVRPLIQVQLS EEQIRNKDEEIQQLRSKLEKVEKERNELRLSSDRLETRISELTSELTDERNTGESASQLLDAETAERLRT EKEMKELQTQYDALKKQMEVMEMEVMEARLIRAAEINGEVDDDDAGGEWRLKYERAVREVDFTKKRLQQE LEDKMEVEQQSRRQLERRLGDLQADSDESQRALQQLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRR FDSELSQAHEETQREKLQREKLQREKDMLLAEAFSLKQQMEEKDLDIAGFTQKVVSLEAELQDISSQESK DEASLAKVKKQLRDLEAKVKDQEEELDEQAGSIQMLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEARQ SCQKKLKQMEVQLEEEYEDKQKALREKRELESKLSTLSDQVNQRDFESEKRLRKDLKRTKALLADAQIML DHLKNNAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEMEDLHLQIDDIAKAKTALEEQLSRLQREKN EIQNRLEEDQEDMNELMKKHKAAVAQASRDMAQMNDLQAQIEESNKEKQELQEKLQALQSQVEFLEQSMV DKSLVSRQEAKIRELETRLEFEKTQVKRLENLASRLKETMEKLTEERDQRAAAENREKEQNKRLQRQLRD TKEEMSELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSE GDSDVDSELEDRVDGVKSWLSKNKGPSKAPSDDGSLKSSSPTSHWKPLAPDPSDDEHDPVDSISRPRFSH SYLSDSDTEAKLTETSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291212
ORF Size	6141 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291212.1, NP_001278141.1
RefSeq Size	7396 bp
RefSeq ORF	6144 bp
Locus ID	360013
UniProt ID	Q9JMH9
MW	232.6 kDa
Gene Summary	May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function]

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