Adgb (NM_001127353) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9130014G24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231856 representing NM_001127353 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCAAACAAGCTAAAAGGAAAGAAGTGCATCGGATCAATTCCGCGCATGGATCAGATAAATCTA AAGATCTCTACCATTTTGGCAGCAATGTGCCACCGGGTTCTTTTGAACAGAAGAAGGGAAAGTTCCCCAT CTGGCCAGAGTGGAGCGAGGCAGACATAAATGCTGAAAAGTGGGATGCAGGCAAAGGCGGGAAGGAGAAG GACAAGACCGCCAAGAGCCCCATCTTTCACTTTTTCGAAGACCCTGAGGGAAAGATTGAATTACCACAGT CATTGAAAGTATTCTCCTGGAAACGTCCGCAAGATTTTATATTTAGCAGAACTCCTGTAGTTGTGAAAAA TGAAATCACATTTGACTTATTTTCACCAAATGAACATTTACTCTGCAGTGAGCTTATGAGGTGGATCATC AGCGAAATCTATGCTGTGTGGAAGATATTCAATGGTGGGATTCTGAGCAATTACCACAAGGGGAATTTGG GGGAGCTTCCTATTCTGCCCTGGAAACCCTGGGAACACATATACTCTCTCTGCAAGGCCGTGAAGGGTCA TGTGCCTTTGTTCAACAGCTACGGGAAGTATGTCGTCAAACTTTACTGGATGGGTTGTTGGAGAAAGATT ACTGTGGATGACTTCCTGCCATTTGATGAGGAGAACAATCTCTTGCTTCCAGCTACATCCTATGAATTTG AACTCTGGCCAATGCTACTGTCGAAAGCTATCATTAAGCTGGCAAATGTTGACGTCCATGTAGCACACAG GAGAGAGCTAGGAGAACTCACAGTAATCCACGCTCTGACTGGGTGGTTGCCTGAAGTCATCCCTCTTCAC CCAGCATATGTGGACAGAGTTTGGGAGCTCTTGAAGGAAATACTGCCTGAGTTTAAGCTCACAGAAGAGC CCAGTTCTGAAAGCAAAATCACAACGATAGACAACAAACTGAAGGAAGATGGCAAGGACGGGAAGGATGG GAAGGATGGCAAGGATGGGAAGGATGGCAAGGACAGCAAGGACTTCAAGTTGGAAGTCTCCCTTACAAAC CTGAAGATTCCCGAGAAGGTCGACAGAGCTGCAAAGGAGAAAGCAGATGCAAGAGACCTTGGGAAGAAGA ATAAGAAGGATGGAGAGAAGGAGAAGGAAAAATTCAAATTTTCACTCCATGGTTCGAGACCCTCATCAGA TGTTCAGTACTCTATGCAGTCCTTGTCAGAGTGTTCCTCAGCAATACAGTTACCTCACATGGTCGTATAT GCCACCTTTACACCACTCTATTTGTTTGAAAACAAAATCTTTTCATTAGAGAAGATGGCGAATTCTGCGG AGAAACTTAGGGAATATGGTCTTTCTCACATCTGTAGCCATCCTGTGCTGGTGACCAGAAGTAGGTCTTG CCCTCTTGTGTCACCCCCCAAACCTCCTCCCTTACCTGCCTGGAAACTCATTCGCCATAAAAAGGAAACA GTGATAACCGATGAAGCTCAAGATGCTGTCCCGAAGAAGCCTGAGCAGTTCCTTGAGATTTCAAGTCCAT TTTTGAATTATAGAATGACTCCATTTACGATTCCAACAGAAACACATTTTGTCCAATCTGTCATTAAGAA AGGAACACCGCTGGGATCCAGTCTTCCTCCCCTCGTGGAAAATGATTTAGTTGCAAGTACCAGCCAAGGG GAAATGAGCATAGTAAATGGAAACCAGTCTCAGGGCAATATTGCATTGCAAATTACACTTGGAAAAGGTA CAGATGAACCTTCAGAGCCTGCATTGGCGGACTTCCACCAACTTGAGGCTACTAGTTTGGACAGGGATTT GATCAGCCTGACTACAGCGACACTGGACAAGTCACAGGAAGAGTTAGCAATAAACGAAGGGGTTGCCAAA GAGATATGGTTAGACTTCGAAGACTTTTGTGTGTGCTTTCACCATATATATATTTTCCACAAGCCACATT CTTATTGTCTTAATTTTCAAAAATCAGAATTCAAGTTTGTGGAAGAGCGTGTGCCTTACTATCTATTTGT GGACAGCCTGAAGCCTATAGAACTACTGGTTTGCTTTTCCGCTTTGGTGCGCTGGGGGGAATCTGGAGCC TTAACAAAAGATAGCCCTCCTGTGGAGCCTGGACTCCTGACAGCTGAAGCCATCACCTGGAAATCCCTGA AGCCTCTTAGTGTTGTTCTGAGAATTCACACCTATGCTACGAAGGCCAGCGTGGTGCGCCTGCCTGCCGG GAGGCATATGCTGCTCTTCAATGCGTATTCCCCTGTGGGCCATGCCATACATGTCTGCAGCATGACAACA TTTGTCATTGGGGATGAAGATATTGTACTGCCCAACTTTGAGCCGGAGAGCTACCGATTTACAGAACAGT CTATAATAATTATGAAAGCTATTGGCAACGTGATTGCTAATTTCAAAGACAAAGGTAAATTGCCTGCAGC TCTGAGGGATCTGCAAGCAGCTCACTACCCTATCCCCCTCAACAACAAAGAACTCACTGCTCAACACTTC CGGGTTTTCCATATATCATTGTGGCGTTTAATGAAAAAATCTCAAGTAGCCAAGCCTCCTTCAAACTTCA AGTTTGCATTTCGGGCTATGGTTTTTGACACGGACTTACTGGATTCTTTCTCAGAGGATGTTTCTTTAGC GGAATGGGTCGACCTTAAATATTCTACACCAATAAATGAGAAGGAGTATACCTCCGAGGAAATAGCTGCT GCGGTGAAAATTCAATCCATGTGGAAAGGGTGCTATGTCAGACTGCTCATGAAAGCCAGAAAGCCAGAAA CAAAGGAAAATGTCACCGTTGCAGATACACTTCAGAAAATTTGGGCAGTATTGGAGATGAATTTGGAGCA ATATGCATTGTCTCTCTTAAGACTAATGTTTAAAAGCAAATGTAAGTCTATGGAATCCTACCCATGCTAT CAAGATGAAGAAACCAAGTTGGCCTTTGCTGACCACACTGTGAACTATGCAGACCAGCCTCCGAATTCTT GGTTTATAGTATTCAGAGAAATATTTTTGGTTCCTCAAGATATGATCATACTCCCCAAAGTGTACACTAC ACTCCCGATCTGTATATTGCATGTCATTAACAATGACACGTTGGAGCAGGTGCCAAAAGTGTTTCAGAAA GTGGTGCCTTTCCTTTACACCAAGAACAAGAAGGGATACACATTTGTGGCTGAAGCATATACTGGTGACA CATTCGTATCAGGAGCACGATGGAAACTGCGGCTCATCGGCTCTTACAACCCGCTCCCGTTTCTCGCCCG TGACTCACCATGCAACACCTTCTCCATAAAGGAAATCAGAGACTACTACATTCCTAATGACAGGAAGATC CTGTTCAGGTACTCCATTAAAGTGACAGTGGCACAGTCTATTACAATCCAGGTCCGCACATCCAAACCCG ACACCTTCATCAAGCTGCAGGTGCTGGAGAGTGAGGAGGTTATCACCAGCACCGTGGGCAAAGGGCAAGC TGTCATCCCTGCCTTCTACTTCCTTGGAAATGAAAAAGCCCTGAGCTCCCAGTCTAGCAAGCAAGTTCTC TTATCTCACCCCTCACCTAAGAAAGATCCAGAAGTGCTGACCAAGAAAAAGTCTGGCCAACCAGGTCAGA AATCCTTCAAAGGAAGATCTGGAGGTGGACTAACTGACACAGGAATGCCCCTGCTGGAAGAAGAAATTTT GAACATACCCACTCTAGAAGAAAATTCTAGCACGCCACAACAGTGCTATAAGTACATCATACAGTGCCTG GTGTTGTTTAACAGCTGGCCTCTAAACGAAACTCAACTGACATTTGTTCAAGCACTAAAAGACATGGAGA AAATGGATATCAAAGCAGAGAAACATGAAGAACCAGCTCCCATGGGAAGCCCAGATTCCCATGCAGTCAG CGAAGGGCAGAAATCTGTAGGAGTGCCCAAAACAACCCGGAAAGGCAAGGAGAAATCTGCCGAGAAAGAA AAGTTAGCTAAGGAAAAGCAAGCTCCTCGATTTGAACCTCAGGTGGTACAAATGCCTACTGCTGTTCACT CTCAGCAAGAAGATCCAAACAAACCCTACTGGATCTTGAGGCTGGTCTCTGAACACACCGACTCAGACTA TGTTGACGTAAAAAAAGACACAGAACGAGCAGATGAAATACGGGCCATGAAGCAAGCTTGGGAGACGACT GAACCGGGGAGGGCCATCAAGGCTGCTCAGGCCCGTTTGAAGTACCTGACCCAATTCATTAAGAAGCCAG TCACCACCGATACCACCACCTCTGCCCCCAGCCCTGAGACTTTGTCTGTGAGTCAAAGCCAAACTAAATC CTCAGAGGAAGGAGAACTAGATACTGGGAAATATGCAGACATCAAAGAACTGCCACCAAACGCAGCAGGA AGTGTGCTGTGGAAGAAGTGGCAAATGACCAAGACCATCACCTCTCTGACAAAATTCACAAGCAGTGAGA GTGTACCGAAGGAAGAGCCACCACAGAAGGAGATCCCTGTGGTCCGACAACGATCACCGACAATTTTGGA AACATCTCCACAACAAATTCGTAAAGCACTAGAGTTTTTGGATTTCAGTCATTATGTACGGAAAACAGCT GCGGAGGCCGTGCTGCAGACAGAGGAACTGAATAAGCAGCAAGCGATGCAGAAGGCAGAAGAAATCCACC AGTTTCGGCAGCACCGCTCCCGCATCCTCAGCATCAGAGACATTGATCAAGAAGAACGGTTTAAGCAAAA GGATGAGGTTCTGGAAATGTATGGAGAGATGCGGGACTCTGTAGATGAAGCCCGGCAGAAGATCCTTGAT ATCCGGGAAGTGTACCGAAACAAGTTGCTGGAGGCAGAGCGCTTGAGGATGGAGGCCTTGGCAGCTCAGG AAGCTGCAGTGAAGATAGAGATAGAGAAGAAGTCACCAGCTTCTGACTCACAGAAGAAGAAGAAAGTAGG AAAGAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231856 representing NM_001127353 Red=Cloning site Green=Tags(s) MASKQAKRKEVHRINSAHGSDKSKDLYHFGSNVPPGSFEQKKGKFPIWPEWSEADINAEKWDAGKGGKEK DKTAKSPIFHFFEDPEGKIELPQSLKVFSWKRPQDFIFSRTPVVVKNEITFDLFSPNEHLLCSELMRWII SEIYAVWKIFNGGILSNYHKGNLGELPILPWKPWEHIYSLCKAVKGHVPLFNSYGKYVVKLYWMGCWRKI TVDDFLPFDEENNLLLPATSYEFELWPMLLSKAIIKLANVDVHVAHRRELGELTVIHALTGWLPEVIPLH PAYVDRVWELLKEILPEFKLTEEPSSESKITTIDNKLKEDGKDGKDGKDGKDGKDGKDSKDFKLEVSLTN LKIPEKVDRAAKEKADARDLGKKNKKDGEKEKEKFKFSLHGSRPSSDVQYSMQSLSECSSAIQLPHMVVY ATFTPLYLFENKIFSLEKMANSAEKLREYGLSHICSHPVLVTRSRSCPLVSPPKPPPLPAWKLIRHKKET VITDEAQDAVPKKPEQFLEISSPFLNYRMTPFTIPTETHFVQSVIKKGTPLGSSLPPLVENDLVASTSQG EMSIVNGNQSQGNIALQITLGKGTDEPSEPALADFHQLEATSLDRDLISLTTATLDKSQEELAINEGVAK EIWLDFEDFCVCFHHIYIFHKPHSYCLNFQKSEFKFVEERVPYYLFVDSLKPIELLVCFSALVRWGESGA LTKDSPPVEPGLLTAEAITWKSLKPLSVVLRIHTYATKASVVRLPAGRHMLLFNAYSPVGHAIHVCSMTT FVIGDEDIVLPNFEPESYRFTEQSIIIMKAIGNVIANFKDKGKLPAALRDLQAAHYPIPLNNKELTAQHF RVFHISLWRLMKKSQVAKPPSNFKFAFRAMVFDTDLLDSFSEDVSLAEWVDLKYSTPINEKEYTSEEIAA AVKIQSMWKGCYVRLLMKARKPETKENVTVADTLQKIWAVLEMNLEQYALSLLRLMFKSKCKSMESYPCY QDEETKLAFADHTVNYADQPPNSWFIVFREIFLVPQDMIILPKVYTTLPICILHVINNDTLEQVPKVFQK VVPFLYTKNKKGYTFVAEAYTGDTFVSGARWKLRLIGSYNPLPFLARDSPCNTFSIKEIRDYYIPNDRKI LFRYSIKVTVAQSITIQVRTSKPDTFIKLQVLESEEVITSTVGKGQAVIPAFYFLGNEKALSSQSSKQVL LSHPSPKKDPEVLTKKKSGQPGQKSFKGRSGGGLTDTGMPLLEEEILNIPTLEENSSTPQQCYKYIIQCL VLFNSWPLNETQLTFVQALKDMEKMDIKAEKHEEPAPMGSPDSHAVSEGQKSVGVPKTTRKGKEKSAEKE KLAKEKQAPRFEPQVVQMPTAVHSQQEDPNKPYWILRLVSEHTDSDYVDVKKDTERADEIRAMKQAWETT EPGRAIKAAQARLKYLTQFIKKPVTTDTTTSAPSPETLSVSQSQTKSSEEGELDTGKYADIKELPPNAAG SVLWKKWQMTKTITSLTKFTSSESVPKEEPPQKEIPVVRQRSPTILETSPQQIRKALEFLDFSHYVRKTA AEAVLQTEELNKQQAMQKAEEIHQFRQHRSRILSIRDIDQEERFKQKDEVLEMYGEMRDSVDEARQKILD IREVYRNKLLEAERLRMEALAAQEAAVKIEIEKKSPASDSQKKKKVGKKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001127353
ORF Size	4980 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001127353.2, NP_001120825.2
RefSeq Size	5218 bp
RefSeq ORF	4977 bp
Locus ID	215772
MW	189.3 kDa

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