Nrxn3 (NM_001198587) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231843 representing NM_001198587 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTTACCCTCCACTCAGTTTTCTTCACCTTGAAGGTGAGCATCTTTCTGGGCTCCCTGGTGGGGC TTTGCCTGGGTCTGGAGTTCATGGGCCTTCCTAACCAGTGGGCCCGCTACCTGCGTTGGGATGCAAGCAC GCGCAGTGACCTGAGCTTCCAGTTCAAGACCAATGTTTCCACTGGGCTGCTCCTGTATTTGGATGATGGT GGTGTCTGTGACTTCCTCTGTCTCTCCCTGGTGGATGGCCGCGTTCAGCTCCGCTTTAGCATGGACTGTG CCGAGACCACTGTGCTATCCAACAAGCAGGTGAACGACAGCAGTTGGCACTTCCTCATGGTGAGCCGTGA TCGTGTGCGCACTGGGCTGGTGATTGATGGTGAGGGCCAGTCTGGAGAGCTACGGCCCCAGCGGCCTTAT ATGGATGTGGTCAGTGATTTGTTCCTCGGTGGCGTCCCCGCTGACATTCGACCTTCTGCCCTGACCCTCG ATGGAGTACAGAGCATGCCTGGCTTTAAGGGATTAATGCTGGATCTCAAGTATGGCAACTCGGAACCTCG GCTTCTGGGGAGCCAGAGCGTCCAGTTAGAAGCAGAGGGACCCTGTGGCGAGCGTCCCTGTGAAAATGGT GGGATCTGCTTCCTCTTGGATGGTCATCCCACCTGTGACTGTTCTACCACTGGCTATGGTGGCACACTCT GCTCAGAAGATGTCAGTCAAGGTCCAGGCCTCTCCCATCTTATGATGAGTGAACAAGCACGAGAGGAGAA CGTGGCCACCTTCCGAGGCTCAGAGTATCTGTGCTATGACCTGTCCCAGAACCCCATCCAGAGCAGCAGC GACGAAATCACCCTTTCCTTTAAGACCTGGCAACGCAATGGGCTCATCCTCCACACTGGCAAGTCGGCCG ACTATGTTAACCTGGCTCTGAAGGATGGTGCGGTCTCCTTGGTCATTAACCTGGGGTCCGGGGCCTTTGA GGCCATTGTGGAGCCAGTGAATGGGAAATTCAACGACAACGCCTGGCATGATGTCAAAGTGACACGCAAT CTTCGGCAGGTGACAATCTCTGTGGATGGCATCCTAACCACAACGGGCTACACTCAAGAGGACTACACCA TGCTGGGCTCAGATGACTTCTTCTATGTTGGTGGAAGCCCAAGTACCGCAGACTTGCCAGGTTCACCTGT GAGCAACAACTTCATGGGCTGCCTTAAAGAGGTTGTTTATAAGAATAATGACATCCGTCTAGAGCTGTCT CGCTTGGCCCGGATTGGAGACACCAAGATGAAAATCTATGGTGAAGTTGTGTTCAAGTGTGAGAACGTGG CCACACTGGATCCCATCAACTTTGAGACCCCAGAGGCTTACATCAGCTTGCCCAAGTGGAACACCAAACG TATGGGCTCCATTTCCTTTGACTTTCGCACCACTGAGCCCAACGGCCTGATTCTCTTCACTCACGGGAAG CCTCAAGAAAGGAAAGATGTCCGGAGCCAAAAGAACACAAAAGTTGACTTCTTTGCTGTGGAACTTCTTG ATGGCAACCTGTATTTGCTGCTTGACATGGGCTCTGGCACCATCAAAGTGAAGGCCACTCAGAAGAAGGC CAATGATGGGGAATGGTACCACGTGGATATTCAGCGTGATGGCAGATCAGGTACCATATCTGTGAACAGC AGGCGCACGCCATTCACCGCCAGTGGGGAGAGTGAAATTCTCGACCTGGAGGGGGACATGTATCTGGGCG GGCTGCCAGAGAACCGAGCTGGCCTCATCCTCCCCACGGAGCTCTGGACCGCCATGCTCAACTACGGTTA CGTGGGCTGCATCCGCGACCTGTTCATCGATGGGCGCAGCAAGAACATCCGGCAGCTGGCGGAGATGCAG AACGCAGCAGGCGTCAAGTCCTCCTGTTCACGCATGAGCGCCAAGCAGTGTGACAGCTACCCCTGCAAGA ACAACGCGGTGTGCAAGGACGGCTGGAACCGCTTCATCTGCGACTGCACTGGCACCGGCTACTGGGGGAG AACCTGCGAAAGAGAGGCATCTATCCTGAGCTATGACGGCAGCATGTACATGAAGGTCATCATGCCCATG GTGATGCACACGGAGGCAGAGGATGTGTCCTTCCGCTTCATGTCACAGCGAGCCTATGGGCTACTGGTAG CCACAACCTCCAGGGATTCTGCAGATACACTGCGTCTAGAGTTGGATGGTGGGCGTGTCAAGCTCATGGT TAACTTAGACTGTATCAGGATAAACTGTAACTCCAGCAAAGGACCTGAGACCCTGTATGCAGGGCAGAAG CTCAATGACAATGAGTGGCACACCGTCCGGGTAGTGCGGAGAGGAAAAAGCCTTAAGCTAACAGTGGATG ATGATGTGGCTGAGGGGACAATGGTGGGCGACCACACCCGCCTGGAATTCCACAATATTGAAACTGGTAT CATGACTGAGAAGCGTTACATCTCTGTGGTCCCCTCCAGTTTCATTGGCCATCTACAGAGCCTCATGTTC AATGGCTTACTGTACATTGATTTGTGCAAAAATGGCGACATCGACTACTGTGAACTGAAGGCTCGCTTTG GACTGAGAAACATCATCGCCGACCCTGTCACTTTTAAGACCAAGAGCAGCTACCTGACCCTCGCCACCCT ACAGGCTTACACCTCCATGCACCTCTTCTTCCAGTTCAAGACCACTTCAGCTGATGGCTTCATTCTCTTC AATAGCGGAGATGGCAATGATTTTATTGCAGTTGAGCTGGTCAAGGGGTACATACACTATGTGTTTGATC TCGGCAATGGTCCCAATGTGATCAAAGGCAACAGTGACCGCCCCCTGAATGACAACCAGTGGCACAACGT GGTCATCACCAGGGACAGCAGTAACACCCACAGTCTGAAGGTGGACACCAAGGTAGTCACTCAGGTCATC AATGGTGCCAAAAATCTGGATTTGAAAGGTGACCTCTATATGGCTGGCTTAGCCCAGGGCATGTACAGCA ACCTTCCCAAGCTTGTGGCCTCCAGGGATGGATTTCAGGGCTGCCTGGCCTCTGTGGACTTGAATGGACG CCTGCCTGATCTCATCAACGATGCTCTCCACAGGAGTGGACAGATCGAGCGAGGCTGTGAAGGACCCAGT ACAACCTGTCAGGAGGATTCATGCGCCAACCAGGGAGTTTGCATGCAGCAGTGGGAAGGTTTCACCTGTG ACTGCTCCATGACATCATATTCTGGAAACCAGTGCAATGACCCTGGTGCAACATACATCTTTGGGAAAAG CGGTGGCCTCATCCTCTATACCTGGCCAGCAAATGACAGACCCAGCACACGCTCTGACCGTCTCGCCGTG GGCTTCAGCACTACTGTGAAGGATGGTATCTTAGTACGCATTGACAGCGCCCCTGGACTTGGCGACTTCC TCCAGCTTCACATAGAACAAGGGAAAATTGGAGTTGTCTTCAATATTGGCACAGTTGACATCTCCATCAA AGAAGAGAGAACTCCTGTCAATGATGGCAAATACCACGTTGTGCGCTTCACCAGGAATGGGGGAAATGCT ACACTTCAGGTGGACAACTGGCCAGTGAATGAGCACTATCCTACAGGCAACACTGATAATGAACGCCTCC AAATGGTAAAACAGAAAATCCCCTTCAAATATAACCGGCCCGTAGAGGAGTGGCTGCAGGAAAAAGGCCG GCAGCTAACCATCTTCAACACCCAGGCGCAAATAGCCATCGGAGGAAAGGACAAAGGACGTCTCTTCCAA GGTCAACTCTCTGGGCTCTATTATGATGGTTTGAAAGTACTGAACATGGCAGCTGAGAACAACCCTAATA TTAAAATCAATGGAAGTGTCCGGCTAGTGGGAGAAGTCCCATCAGTCTCAGGAACAACACAGACAACGTC CATGCCACCTGAAATGTCTACCACCGTCATGGAAACCACCACCACCATGGCTACGACCACCACCCGAAAG AACCGCTCTACAGCCAGCATTCAGCCCACGTCAGATGATCTTGTTTCATCTGCTGAATGTTCAAGTGATG ATGAAGACTTTGTCGAATGTGAACCAAGTACAGATAAGAGTCTTTCCACTTCAATCTTCGAAGGTGGCTA CAAAGCACATGCGCCCAAGTGGGAATCCAAGGACTTTAGACCTAACAAAGTCTCGGAAACTAGTAGAACT ACAACCACCTCTTTGTCCCCTGAGCTGATCCGCTTCACAGCGTCCTCCTCGTCTGGGATGGTGCCCAAAT TGCCAGCTGGCAAAATGAATAACCGTGATCTCAAACCCCAGCCTGATATAGTCTTGCTTCCGTTGCCCAC TGCCTATGAGCTAGACAGCACCAAACTGAAGAGCCCACTAATTACTTCCCCCATGTTCCGTAATGTGCCC ACAGCAAACCCCACGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAGAGGTGATCCGGGAGTCCAGCA GTACAACAGGGATGGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCATCTTGATCCTCCTGTACGCCAT GTACAAGTACAGGAACAGGGACGAGGGGTCCTATCAAGTGGACGAGACGAGGAACTACATCAGCAACTCG GCCCAGAGCAACGGCACGCTCATGAAGGAGAAGCAAGCCAGCTCCAAGAGCGGCCATAAGAAACAGAAAA ACAAGGACAAGGAGTATTATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231843 representing NM_001198587 Red=Cloning site Green=Tags(s) MSFTLHSVFFTLKVSIFLGSLVGLCLGLEFMGLPNQWARYLRWDASTRSDLSFQFKTNVSTGLLLYLDDG GVCDFLCLSLVDGRVQLRFSMDCAETTVLSNKQVNDSSWHFLMVSRDRVRTGLVIDGEGQSGELRPQRPY MDVVSDLFLGGVPADIRPSALTLDGVQSMPGFKGLMLDLKYGNSEPRLLGSQSVQLEAEGPCGERPCENG GICFLLDGHPTCDCSTTGYGGTLCSEDVSQGPGLSHLMMSEQAREENVATFRGSEYLCYDLSQNPIQSSS DEITLSFKTWQRNGLILHTGKSADYVNLALKDGAVSLVINLGSGAFEAIVEPVNGKFNDNAWHDVKVTRN LRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDIRLELS RLARIGDTKMKIYGEVVFKCENVATLDPINFETPEAYISLPKWNTKRMGSISFDFRTTEPNGLILFTHGK PQERKDVRSQKNTKVDFFAVELLDGNLYLLLDMGSGTIKVKATQKKANDGEWYHVDIQRDGRSGTISVNS RRTPFTASGESEILDLEGDMYLGGLPENRAGLILPTELWTAMLNYGYVGCIRDLFIDGRSKNIRQLAEMQ NAAGVKSSCSRMSAKQCDSYPCKNNAVCKDGWNRFICDCTGTGYWGRTCEREASILSYDGSMYMKVIMPM VMHTEAEDVSFRFMSQRAYGLLVATTSRDSADTLRLELDGGRVKLMVNLDCIRINCNSSKGPETLYAGQK LNDNEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNIETGIMTEKRYISVVPSSFIGHLQSLMF NGLLYIDLCKNGDIDYCELKARFGLRNIIADPVTFKTKSSYLTLATLQAYTSMHLFFQFKTTSADGFILF NSGDGNDFIAVELVKGYIHYVFDLGNGPNVIKGNSDRPLNDNQWHNVVITRDSSNTHSLKVDTKVVTQVI NGAKNLDLKGDLYMAGLAQGMYSNLPKLVASRDGFQGCLASVDLNGRLPDLINDALHRSGQIERGCEGPS TTCQEDSCANQGVCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPANDRPSTRSDRLAV GFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIKEERTPVNDGKYHVVRFTRNGGNA TLQVDNWPVNEHYPTGNTDNERLQMVKQKIPFKYNRPVEEWLQEKGRQLTIFNTQAQIAIGGKDKGRLFQ GQLSGLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSVSGTTQTTSMPPEMSTTVMETTTTMATTTTRK NRSTASIQPTSDDLVSSAECSSDDEDFVECEPSTDKSLSTSIFEGGYKAHAPKWESKDFRPNKVSETSRT TTTSLSPELIRFTASSSSGMVPKLPAGKMNNRDLKPQPDIVLLPLPTAYELDSTKLKSPLITSPMFRNVP TANPTEPGIRRVPGASEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYQVDETRNYISNS AQSNGTLMKEKQASSKSGHKKQKNKDKEYYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198587
ORF Size	4713 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198587.3, NP_001185516.2
RefSeq Size	7933 bp
RefSeq ORF	4716 bp
Locus ID	18191
UniProt ID	Q6P9K9
MW	173.9 kDa
Gene Summary	This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. [provided by RefSeq, Dec 2012]

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