Tex14 (NM_001199293) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C85585
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231808 representing NM_001199293 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACCCTGTCTACAAACAAACAAACAAAACAACATGTCTCGGGGGGCTCCTTTTCCAGTCCCTTGCC CGGTGCTGCTGGGGACCTTCACAGATGACTCCCTGGAGGCTCAGCTTCACGAATACGCCAAGCAAGGGAA CTGCGTGAAGCTCAAGAAGATTCTTAAGAAGGGAGTCTGTGTCGATGCAGTTAACACTCAGGGCCAGTCA GCGCTCTTTGTTGCAGCATTACTGGGCCATGTGAAATTAGTGGATGTTCTGGTGGATTATGGATCAGATC CAAATCACCGCTGCTTTGACGGGAGCACTCCTGTCCATGCAGCAGCCTTTTCAGGCAACCAGTGGATCCT CAGTAAACTGCTGACTGCAGGGGGTGACCTGCGACTCCATGATGAGAAGGGTCGGAATCCACAGGCCTGG GCCTTGACAGCAGGGAAGGATCGTAGCACTCAGATGGTGGAGTTCATGCAACGTTGTACTTCACATATGA AGGCTATCATCCAGGGCTTCTCCTATGACCTTCTGAAGAAGATAGACTCTCCTCAGCGACTCATCGGCAG CCCACCCTGGTTTGGTAGCCTCATTCAGGGAAGCCCAAATAGTTCTCCCAACCGACAGCTTAAACCTGGA ATCATTTCTGCCCAAAATATCTACAGCTTTGGCTTTGGGAAGTTTTATCTCACCTCAGGGATGCAGCTTA CTTACCCAGGATCTCTTCCAGTCATTGGAGAGAAGGAAGTGGTCCAGGCTGATGATGAGCCCACCTTCTC TTTCTTCAGTGGCCCCTACATGGTCATGACTAACCTCGTGTGGAATAGGAGCAGAGTCACAGTAAAGGAG CTGAACCTTCCCACCCGTCCCCACTGTAGCAGGCTGAGGTTGGCCGACTTGCTGATTGCTGAGCAGGAGC ACAGCAGCAACCTGCGGCATCCTAACCTGCTGCAACTGATGGCTGTATGTTTGTCCCGGGACCTGGAGAA AATTCGCCTGGTTTACGAGCGTATCACAGTCGGCACACTGTTCAGTGTCCTCCATGAACGAAGGTCCCAG TTCCCAGTGCTGCACATGGAGGTGATTGTGCACCTGTTGCTCCAGGTTGCTGATGCCTTGATATACCTGC ATTCCCGGGGGTTCATCCACCGCTCCCTCAGCTCCTACGCTGTCCACATCGTCTCTGCAGGAGAAGCAAG GCTGACTAACCTGGAATACCTGACGGAAAGCCAGGACAGTGGTGCACACAGGAACGTGACTCGAATGCCC CTCCCCACCCAGCTGTACAACTGGGCTGCACCAGAAGTGGTCTTGCAGAAGGCAGCCACGGTGAAGTCAG ACATATACAGCTTTTCCGTGATCATACAAGAGATCTTAACAGACAGTATACCCTGGAATGGCTTGGATGG CTCACTTGTTAAAGAAACCATAGCCTTGGGAAATTATTTAGAAGCTGATGTCAGGCTTCCGGAACCTTAC TATGATATTGTTAAGTCAGGAATCCATGCCAAGCAGAAGAACCGAACAATGAACCTTCAAGATATTCGTT ATATTCTGAAGAATGACTTAAAGGAATTTATTGGAGCTCAGAAAACTCAGCCAACCGAGAGCCCCAGAGG GCAGAGCTATGAACCCCATCCTGATGTTAATATCTGCCTAGGTCTAACTTCAGAATATCAAAAGGACCCT CCAGACTTGGACATCAAGGAACTAAAGGAAATGGGTAGTCAGCCCCATTCACCTACAGATCACTCCTTTC TCACTGTAAAACCAACACTAGCTCCTCAGACCCTAGATTCAAGTCTGTCAGCCCAGAAACCTGACAATGC AAATGTTCCTTCTCCTCCTGCTGCATGTCTGGCAGAAGAGGTCAGGAGCCCCACTGCAAGTCAGGACAGC CTCTGCAGCTTTGAAATCAATGAGATCTACTCAGGCTGCTTGACACTGGGAACTGACAAGGAGGAAGAGT GTCTGGGGACTGCTGCTTCACCTGAGGGGGATAGACCAAACCAGGGAGATGAGCTGCCATCCCTGGAAGA AGAGCTCGATAAGATGGAGAGAGAATTGCACTGTTTTTGTGAAGAGGACAAAAGCATTTCAGAAGTTGAC ACAGACCTTCTTTTTGAGGATGATGACTGGCAAAGTGATTCTCTTGGTTCACTCAACCTGCCCGAACCAA CCAGAGAAGCCAAGGGCAAAACGAGCAGCTGGTCCAAGACTGATGAGTATGTCAGTAAGTGTGTGCTGAA TCTGAAGATTTCACAGGTGATGATGCAGCAGAGCGCTGAGTGGCTGAGGAAGCTTGAGCAGGAGGTAGAG GAGCTCGAGTGGGCACAGAAGGAGCTGGACAGTCAGTGCAGCAGTTTGCGGGATGCTTCATTAAAGTTTG CAAATGCCAAGTTCCAGCCGGCTGTAGGCCCTCCATCTTTGGCCTATCTTCCTCCTGTTATGCAATTACC AGGGCTCAAGCAGCCTGAAAATGGTGGCACCTGGTTAACCCTAGCAAGGTCTCCAGGAAATGAGAGAGAG TTCCAAGAGGGACATTTTAGCAAAAAACCTGAGAAACTAAGTGCCTGTGGCTGGAAGCCTTTTACACAAG TGTCTGAAGAAAGCAGAGGGGACTGCTCAGAGCTAAACAATCAGCTGCCGACTCTTCGTGGTCCTGGGAA GCAGAGCACAGGTGAGCAGTTACCATCCACTCAAGAAGCAAGGGAGAGTTTGGAAAAAAATACAAACCAA AATAGTAGGAGTATGGCGTCTGTGTCTTCTGAAATCTATGCTACTAAGTCAAGAAATAATGAGGATAATG GAGAGGCACACTTGAAATGGAGATTGGCAGTAAAAGAAATGGCAGAGAAAGCAGTTTCCGGACAGCTCTT ATTACCTCCTTGGAATCCTCAGAGTAGTGCGCCTTTTGAGAGTAAGGTTGAAAATGAGAGCACTCCTTTG CCACGGCCCCCAATTAGAGGTCCTGAGAGCACAGAATGGCAGCACATTTTAGAATACCAGAGGGAAAATG ATGAGCCCAAAGGAAATACGAAGTTTGGCAAAATGGACAACAGTGACTGTGACAAGAACAAGCACAGCAG ATGGACAGGCCTCCAGCGCTTCACTGGTATTAGATACCCATTCTTCAGAAACCACGAGCAGCCAGAGCAG AATGAAGCCTCTCAAGCAAGCTGTGACACGTCTGTGGGCACTGAGAAGTTCTACAGCACCTCAAGTCCCA TAGGAGACGACTTTGAAAGATTCCAAGATTCTTTTGCCCAACGTCAAGGCTATGTTGAAGAAAATTTCCA AATAAGAGAAATATTTGAAAAGAATGCTGAGATTTTGACCAAGCCTCAGTTTCAAGCTATTCAATGTGCT GAAGACAAACAAGACGAAACATTAGGGGAGACGCCAAAGGAACTGAAAGAGAAAAACACATCACTGACAG ACATTCAAGACTTGTCCAGCATCACCTATGATCAAGACGGCTATTTTAAGGAAACCTCATACAAAACACC CAAATTAAAACACGCACCAACTAGTGCCAGTACCCCGCTAAGCCCAGAGTCGATTTCTTCAGCTGCTAGT CACTATGAAGACTGCCTTGAAAATACCACATTTCATGTTAAAAGAGGATCTACATTTTGTTGGAATGGCC AAGAAGCTATGAGAACTTTGTCTGCCAAATTTACAACTGTCCGAGAGAGAGCTAAGAGCCTGGAATCACT TCTCGCTTCTTCTAAAAGCCTACCTGCCAAGCTGACTGACTCCAAGAGATTGTGTATGTTGAGTGAGACT GGCTCTTCTAACGTTTCTGCGGCATTTGTAACATCAACTCATGCTACCAAGAGGAAGAGCCTACCCAGAG AACTGGCAGAAGCCACCTCTCAACAGCATCTTGATGAGCTTCCACCACCAGCTCAGGAGCTACTTGATGA AATTGAGCAACTGAAGCAGCAGCAGGTCTCATCCCTGGCGTCACATGAGAACACGGCACGTGATCTGAGT GTCACTAACAAGGATAAGAAGCATTTGGAAGAACAAGAAACCAACAGTAGTAAAGACAGCAGTTTTCTTT CCAGCAGAGAAATTCAGGATCTGGAAGATACAGAGAGAGCTCATTCTTCTCTTGATGAGGACCTGGAAAG ATTCCTGCAGTCACCTGAGGAGAACACGGCACTGCTGGACCCTACCAAGGGCTCTACAAGGGAGAAAAAA AACAAAGATCAAGACGTTGTTGAGCAGAAGAGAAAAAAGAAAGAAAGCATCAAGCCAGAGAGAAGGGAGT CAGACAGCTCCCTAGGGACCTTGGAAGAAGATGAACTAAAACCCTGTTTTTGGAAGCGACTGGGTTGGTC CGAACCTTCCAGGATAATTGTGCTGGATCAGAGCGACTTGTCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231808 representing NM_001199293 Red=Cloning site Green=Tags(s) MRPCLQTNKQNNMSRGAPFPVPCPVLLGTFTDDSLEAQLHEYAKQGNCVKLKKILKKGVCVDAVNTQGQS ALFVAALLGHVKLVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLTAGGDLRLHDEKGRNPQAW ALTAGKDRSTQMVEFMQRCTSHMKAIIQGFSYDLLKKIDSPQRLIGSPPWFGSLIQGSPNSSPNRQLKPG IISAQNIYSFGFGKFYLTSGMQLTYPGSLPVIGEKEVVQADDEPTFSFFSGPYMVMTNLVWNRSRVTVKE LNLPTRPHCSRLRLADLLIAEQEHSSNLRHPNLLQLMAVCLSRDLEKIRLVYERITVGTLFSVLHERRSQ FPVLHMEVIVHLLLQVADALIYLHSRGFIHRSLSSYAVHIVSAGEARLTNLEYLTESQDSGAHRNVTRMP LPTQLYNWAAPEVVLQKAATVKSDIYSFSVIIQEILTDSIPWNGLDGSLVKETIALGNYLEADVRLPEPY YDIVKSGIHAKQKNRTMNLQDIRYILKNDLKEFIGAQKTQPTESPRGQSYEPHPDVNICLGLTSEYQKDP PDLDIKELKEMGSQPHSPTDHSFLTVKPTLAPQTLDSSLSAQKPDNANVPSPPAACLAEEVRSPTASQDS LCSFEINEIYSGCLTLGTDKEEECLGTAASPEGDRPNQGDELPSLEEELDKMERELHCFCEEDKSISEVD TDLLFEDDDWQSDSLGSLNLPEPTREAKGKTSSWSKTDEYVSKCVLNLKISQVMMQQSAEWLRKLEQEVE ELEWAQKELDSQCSSLRDASLKFANAKFQPAVGPPSLAYLPPVMQLPGLKQPENGGTWLTLARSPGNERE FQEGHFSKKPEKLSACGWKPFTQVSEESRGDCSELNNQLPTLRGPGKQSTGEQLPSTQEARESLEKNTNQ NSRSMASVSSEIYATKSRNNEDNGEAHLKWRLAVKEMAEKAVSGQLLLPPWNPQSSAPFESKVENESTPL PRPPIRGPESTEWQHILEYQRENDEPKGNTKFGKMDNSDCDKNKHSRWTGLQRFTGIRYPFFRNHEQPEQ NEASQASCDTSVGTEKFYSTSSPIGDDFERFQDSFAQRQGYVEENFQIREIFEKNAEILTKPQFQAIQCA EDKQDETLGETPKELKEKNTSLTDIQDLSSITYDQDGYFKETSYKTPKLKHAPTSASTPLSPESISSAAS HYEDCLENTTFHVKRGSTFCWNGQEAMRTLSAKFTTVRERAKSLESLLASSKSLPAKLTDSKRLCMLSET GSSNVSAAFVTSTHATKRKSLPRELAEATSQQHLDELPPPAQELLDEIEQLKQQQVSSLASHENTARDLS VTNKDKKHLEEQETNSSKDSSFLSSREIQDLEDTERAHSSLDEDLERFLQSPEENTALLDPTKGSTREKK NKDQDVVEQKRKKKESIKPERRESDSSLGTLEEDELKPCFWKRLGWSEPSRIIVLDQSDLSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199293
ORF Size	4386 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199293.1, NP_001186222.1
RefSeq Size	4795 bp
RefSeq ORF	4389 bp
Locus ID	83560
UniProt ID	Q7M6U3
MW	164.4 kDa
Gene Summary	Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro.[UniProtKB/Swiss-Prot Function]

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