Arap3 (NM_001205336) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Centd; Centd3; cnt-d3; DRA; Drag1; E030006K04Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231807 representing NM_001205336 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCAACTCCCAGCCCAGCCCCTGATGCCCAACCCCCCAAGCCTGTGCCTAAGCCCAGGACGGTGT TTGGGCTCAGCAACCCTGCCACTGCCCAGAGGCCTGGACTGAGCCCAATCTTCTGGGATCCAGAAGTATC CAGGAACTCAGAGTGTACGCAAAGATCCTCTCCTCTCCTTCCTTCCTCCTCTGAACAGCCTTCAGTCCCG AATACCATGGAGATGATGCCTAATGCCATCTACTTTGGCCTGGACTTAAGAGGTCGGGCCCAGGCAGCTC AGGACGTGACTCCAGATAGTTCTCAGGCGACTGTCCCAACCCCTGCCTTCAGGCCCACAACAGGCACAGT GCACATCATGGATCCTGGTTGCCTGTACTACGGTGTCCAGCCTGTGGGGATCCCAGGAGCCTCTGACAGG AGAGACGGCAGGGGTGTCTGTCAGGAAAGGGCAGAACACAGACAGGATCTGGAGACACGGGAGGATGCTG GTTATGCCAGCCTCGAGCTACCTGGAGACTCCATTCTTTCGCTACCCACCCAGGATGCAGAGACCAGTGA TGACCTCATTTCGCCCTATGCCAGCTTCTCCTCCACAGCAGATCGTCCCGTGCCTCTGCTCAGTGGCTGG CTGGACAAGCTCTCACCTCAGGGAAACTATGTGTTCCAGAGACGTTTTGTGCAGTTCAATGGAAGGAGTC TAATGTACTTTGGCAGTGACAAGGACCCCTTCCCCAAAGGTGTGATCCCTCTGACTGCCATCGAGATGAC CCGAAGCAGCAAGGACAACAAATTCCAGGTCATTACCGGCCAGAGAGTATTTGTGTTCCGCACAGAGAGT GAGGCTCAGCGTGACCTATGGTGCTCCACGCTGCAATCCTGCCTGAAGGAACAGCGCCTCCTGGGCCACC CCCGGCCCCCTCACCCACCACGACCCCTCCGCACGGGCACGTTAGAGTTGCGTGGACACAAGGCCAAGGT GTTTGCTGCCTTGATCCCTGGAGAGCTGGCACTGTACAAGAGTGAGCAGGCCTTCTCACTGGGCATCGGG ATCTGCTTCATTGAACTGCAAGGCTGCAGTGTCCGGGAGACCAAGAGTCGAAGCTTCGATCTGCTCACAC CCCATCGCTGCTTCAGCTTCACAGCCGAGTCTGGGGGGGCTCGACAGAGCTGGGCAGCCGCTCTGCAAGA AGCAGTAACCGAGACACTGTCTGACTACGAGGTGGCTGAGAAGGTCTGGTCCAACCCGGCAAACCGGCAT TGTGCGGACTGCAGGGCCTCCCGGCCGGATTGGGCTGCCGTCAACCTGGGGGTCGTCATCTGCAAGCAGT GTGCAGGTCAGCACAGGGCCCTGGGCTCTGGGATCTCCAAGGTGCAGAGCCTGAAGCTGGACACGAGTGT CTGGAGTAATGAGATAGTGCAGCTGTTCATTGTCCTGGGAAATGATCGTGCCAATTGCTTCTGGGCAGGG GCACTTCCCCCAGGTGAAGGGCTGCATCCAGATTCAGCCCCTGGCCCTCGAGGAGAGTTCATCTCCCGAA AGTATAAGCTGGGTCTCTTCCGGAAGCCCCACCCTCGGCATCCAGATCATAGCCAGCTTCTCCAGGCACT GTGTGCAGCTATGGCAGGACCCAACCTGCTCAAGAACATGGCCCAGCTTCTCTGTGTCGAAACCTCTGAG GGAGAGGAACCCTTGTCCCCTTCAGCCCTCAATGGCAGCTTGCTTAGCCTTCTACCTTCAGACTCCCCTG GAGTATACAATGAGGTGGTAGTGCCTGCCACTTACAGAGGTTTCCTGTACTGTGGCTCCATCAGCAACAA GGCTGGAGCCCCACCCCTTCGCAGGGGCCGGGATGCTCCTCCCCGCTTGTGGTGTGTGCTGGGAGCTGCC CTGGAAATGTTTGCATCAGAGAGCAGCCCTGAACCCCTCAGCCTTCTCCAGCCCCAGGATATTGTATGTC TGGGTGTAAGCCCCCCACCTGCTGATCCAGGTGACCTTGACAGGTTCCCCTTTTCCTTTGAACTCATCCT CACTGGGGGAAGAATCCAGCATTTTGCCACTGATGGAGCAGACAGTCTAGAGGCCTGGATCAGTGCAGTG GGCAAGTGGTTCTCCCCGCTCAGCTGCCACCAGCTGCTGGGCCCTGGGTTGCTGCGGATGGGTCGCCTGT GGCTACGGTCCCCCTCCCACGCAGGCCTGGCCCCTGGTCTCTGGCTGTCGGGCTTCGGCCTCCTTCGTGG TGATCATCTCTTCCTGTGCCCAGCGCCTGGCCCTGGTCCCCCTGCTCCGGAGGACATGGTGCATTTACGA CGTCTACAGGAGATCAGTGTGGTCTCGGCAGCTGACACTCCAGACAAAAAGGAGCATTTGGTCCTTGTAG AGACAGGAAGGACCCTGTATTTGCAGGGGGAAGGCAGGCTAGACTTTGCAGCGTGGAACACGGCCATTGG AGGTGCTGCCGGAGGGGGTGGCACGGGCCTGCAGGAGCAGCAGATGAGCCGGGGAGACATCCCTATCATT GTGGACGCTTGCATCAGTTTTGTCACGCAGCATGGGCTCCGGCTGGAAGGTGTATATCGGAAAGGAGGCG CTCGAGCCCGCAGCCTGCGACTCTTGGCTGAGTTTAGGCGGGATGCCCGGTCAGTGAAGCTTAGACCACG GGAACACTTTGTAGAAGATGTTACTGACACACTCAAACGCTTCTTTCGAGAGCTTGATGACCCCGTGACC TCTGCTAGGTTGCTGCCTCGATGGAGAGAAGCTGCAGAGCTTTCCCAGAAGAATCAGCGCCTGGAGAAGT ACAAAGAAGTGATTAGCTGCCTGCCTCGAGTCAACCGCAGAACACTGGCCACCCTCATCGGGCATCTGTA CCGGGTGCAGAAGTGTGCGTCTCTAAACCAGATGTGCACGCGGAACCTGGCCCTACTGTTTGCACCTAGT GTGTTCCAGACAGATGGGCGGGGAGAGCATGAGGTTCGGGTACTGCAGGAGCTCATCGATGGCTACATCT CTGTTTTTGATATTGACTCGGACCAGGCAGCTCAGATTGACTTGGAGGTCAGTCTCATTACCACCTGGAA GGATGTGCAGTTGTCCCAGGCTGGAGACCTCATCATGGAGGTTTACATAGAACAGCAGCTCCCGGACAAC TGTGTCACCCTAAAGGTATCCCCAACACTGACTGCTGAGGAGTTGACTAACCAGGTGCTGGAGATGCGAG GGGCGGCATCGGGGACAGACTTGTGGGTGACATTTGAAATTCTTGAACACGGAGAGCTTGAGAGGCCACT GCACCCCAAAGAAAAGGTTCTAGAGCAGGCCTTGCAGTGGTGCCAGCTTCCAGAGCCCTGCTCAGCCTCT CTGCTTCTGAGAAAAGTCTCTATGGCACACGCTGGCTGCCTCTTCACAGGAGTCCGGCGTGAGAGCCCAC GAGTGGGGCTGCTACGGTGTCGTGAGGAGCCACCTCGATTGCTGGGAAACCGTTTCCAGGAGAGGTTCTT TTTGGTGCGTGGCCGATGCCTGCTGCTGCTCAAGGAGAAGAAGAGCTCTAAGCCAGAACGGGAGTGGTCT CTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCAAGAAGTTAAAACCTCCAACACTGTGGGGTTTCACAT TAATATTGGAAAAGATGCACCTCTGTCTGTCCTGCATGGATGAGGAGGAGATGTGGGACTGGACCACAAG CATCCTTAAAGCCCAGCATGATGACCAACAGTCCGTGGTTTTACGTCGCCGTTCCTCCTCTGACCTTGCT CGTCAGAAGTTTGGCACTATGCCCTTGCTGCCCATCCGAGGGGATGACAGTGGAGCCACCCTTCTCTCTG CCAATCAGACCCTGCGGCGACTACACAACCGGAGGACCTTATCCATGTTCTTTCCAATGAAGTCACCCCA GGGATCTGTGGAAGAGCAAGATGAGCTGGAGGAGCCTGTGTATGAAGAGCCAGTATATGAGGAGGTCGGG GCCTTCCCTGAGCTTACCAAGGACACGACCTTCTCTTCCACATGGGAGTGGTCAGCCAAGTCAGATCCAT CCCTTACCAGCCAGAGGTCCTTTGATCAACCCCCCCTCTCCAAGGCAAGCATGTTGGGCCACGAAGAGAG GATACCTGACCCTCCTCCGGGCCCCCCTTCAAAAAGCAGTTCTCAGGCACGAGGGTCCCTAGAGGAACAG CTGCTTCAGGAACTCAACAACCTCATCCTGAGGAAAGGAGAGCCTGCCTCGTGCCCAGAAAGCTCTTCCC AGCCCACCAGTCCCCAAGCCCCAAGCCCCACCAGCCTTCCAACACCGACCCCCAGCTTACCCACCCAACC GCCCTGCACTTCCAATCCACCCTCCAGCCAGCCTCTCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231807 representing NM_001205336 Red=Cloning site Green=Tags(s) MEPTPSPAPDAQPPKPVPKPRTVFGLSNPATAQRPGLSPIFWDPEVSRNSECTQRSSPLLPSSSEQPSVP NTMEMMPNAIYFGLDLRGRAQAAQDVTPDSSQATVPTPAFRPTTGTVHIMDPGCLYYGVQPVGIPGASDR RDGRGVCQERAEHRQDLETREDAGYASLELPGDSILSLPTQDAETSDDLISPYASFSSTADRPVPLLSGW LDKLSPQGNYVFQRRFVQFNGRSLMYFGSDKDPFPKGVIPLTAIEMTRSSKDNKFQVITGQRVFVFRTES EAQRDLWCSTLQSCLKEQRLLGHPRPPHPPRPLRTGTLELRGHKAKVFAALIPGELALYKSEQAFSLGIG ICFIELQGCSVRETKSRSFDLLTPHRCFSFTAESGGARQSWAAALQEAVTETLSDYEVAEKVWSNPANRH CADCRASRPDWAAVNLGVVICKQCAGQHRALGSGISKVQSLKLDTSVWSNEIVQLFIVLGNDRANCFWAG ALPPGEGLHPDSAPGPRGEFISRKYKLGLFRKPHPRHPDHSQLLQALCAAMAGPNLLKNMAQLLCVETSE GEEPLSPSALNGSLLSLLPSDSPGVYNEVVVPATYRGFLYCGSISNKAGAPPLRRGRDAPPRLWCVLGAA LEMFASESSPEPLSLLQPQDIVCLGVSPPPADPGDLDRFPFSFELILTGGRIQHFATDGADSLEAWISAV GKWFSPLSCHQLLGPGLLRMGRLWLRSPSHAGLAPGLWLSGFGLLRGDHLFLCPAPGPGPPAPEDMVHLR RLQEISVVSAADTPDKKEHLVLVETGRTLYLQGEGRLDFAAWNTAIGGAAGGGGTGLQEQQMSRGDIPII VDACISFVTQHGLRLEGVYRKGGARARSLRLLAEFRRDARSVKLRPREHFVEDVTDTLKRFFRELDDPVT SARLLPRWREAAELSQKNQRLEKYKEVISCLPRVNRRTLATLIGHLYRVQKCASLNQMCTRNLALLFAPS VFQTDGRGEHEVRVLQELIDGYISVFDIDSDQAAQIDLEVSLITTWKDVQLSQAGDLIMEVYIEQQLPDN CVTLKVSPTLTAEELTNQVLEMRGAASGTDLWVTFEILEHGELERPLHPKEKVLEQALQWCQLPEPCSAS LLLRKVSMAHAGCLFTGVRRESPRVGLLRCREEPPRLLGNRFQERFFLVRGRCLLLLKEKKSSKPEREWS LEGAKVYLGIRKKLKPPTLWGFTLILEKMHLCLSCMDEEEMWDWTTSILKAQHDDQQSVVLRRRSSSDLA RQKFGTMPLLPIRGDDSGATLLSANQTLRRLHNRRTLSMFFPMKSPQGSVEEQDELEEPVYEEPVYEEVG AFPELTKDTTFSSTWEWSAKSDPSLTSQRSFDQPPLSKASMLGHEERIPDPPPGPPSKSSSQARGSLEEQ LLQELNNLILRKGEPASCPESSSQPTSPQAPSPTSLPTPTPSLPTQPPCTSNPPSSQPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205336
ORF Size	4380 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205336.1, NP_001192265.1
RefSeq Size	5425 bp
RefSeq ORF	4383 bp
Locus ID	106952
UniProt ID	Q8R5G7
MW	161.6 kDa
Gene Summary	This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

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