Ssh2 (NM_001291190) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mSSH-2L; SSH-2; SSH-2L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231800 representing NM_001291190 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGAGCACTTTGGCAGGCGAAAGGAAAGCGCTCCCTGCCAGCACCTGCAGCCTCGGTGGCCCCG ACATGATCCCCTACTTCTCTGCCAATGCGGTCATCTCGCAGAACGCCATCAACCAGCTCATCAGCGAGAG CTTCTTAACTGTCAAAGGTGCTGCCCTTTTTCTACCACGAGGGAATGGCTCGTCTACACCAAGAGTCAGC CACAGACGCAACAAGCATGCAGGTGATCTCCAACAGCATCTTCAAGCAATGTTCATATTACTCCGCCCAG AAGACAACATAAGGCTGGCTGTAAGACTGGAAAGTACTTACCAAAACCGAACACGCTATATGGTCGTGGT TTCAACTAATGGTAGACAAGACACTGAAGAAAGCATTGTCCTGGGAATGGATTTTTCTTCTAATGACAGC ACTTGTACCATGGGCTTGGTCTTGCCTCTCTGGAGTGACACCCTAATTCATTTGGATGGTGATGGTGGGT TCAGCGTATCAACAGATAACAGAGTTCACATATTCAAACCTGTATCTGTGCAAGCAATGTGGTCTGCACT ACAGAGTTTGCACAAGGCTTGTGAAGTGGCCAGAATGCATAACTACTATCCAGGCAGCCTTTTTCTCACT TGGGTGAGTTATTACGAGAGCCATATCAACTCAGATCAGTCCTCAGTCAATGAGTGGAATGCTATGCAGG ATGTGCAGTCTCATCGGCCTGACTCTCCAGCCCTCTTCACAGACATACCAACTGAACGTGAGCGAACAGA AAGGCTTATTAAGACTAAATTAAGGGAGATCATGATGCAAAAGGATTTGGAGAATATCACCTCCAAAGAG ATACGCACGGAGCTGGAGATGCAGATGGTGTGCAACTTGCGAGAATTTAAGGAATTCATAGATAATGAAA TGATCGTGATCCTTGGTCAGATGGACAGTCCCACACAGATATTTGAGCATGTATTCCTGGGCTCAGAATG GAATGCCTCGAACTTAGAGGACTTGCAGAACCGAGGGGTACGGTATATCTTGAATGTTACTCGAGAGATA GATAACTTCTTCCCTGGAGTCTTTGAGTATCATAACATTCGAGTATATGATGAAGAAGCAACAGATCTCC TGGCTTACTGGAATGACACTTACAAATTCATCTCTAAAGCAAAGAAACATGGATCTAAATGTCTTGTGCA CTGCAAAATGGGGGTGAGTCGCTCAGCCTCCACTGTGATTGCCTATGCAATGAAGGAGTATGGATGGAAT CTGGATCGAGCCTATGACTATGTGAAGGAAAGACGAACGGTGACCAAGCCTAACCCCAGCTTCATGAGAC AACTGGAAGAATACCAAGGGATCTTGCTGGCCAGCAAACAGCGGCATAACAAACTCTGGAGATCTCATTC AGATAGTGACCTTTCAGACCACCACGAACCCATCTGTAAACCTGGGCTCGAGCTCAACAAGAAGGAGATG ACCACGTCAGCAGACCAGATCGCTGAGGTGAAGACTGTGGAGAACCTTGCTGCCATGCCTACCGTCTTTA TGGAACATGTGGTCCCACAAGATGCAAATCAGAAAGGACTACACACCAAAGAAAGAGTCATCTGCTTGGA GTTTTCTTCACAAGAGTTTCGTGCCGGACAGATTGAAGATGAATTAAATTTAAATGACATCAATGGATGC TCATCAGGGTGTTGTCTCAGTGAATCAAAACTCCCTCTTGACAACTGCCATGCGTCTAAAGCCTTACTCC AACCTGGACAGGCCCCAGACATTGCCAACAAGTTCCCAGACTTAGCTGTGGAAGATCTGGAGACAGACGC ACTGAAAGCAGACATGAATGTCCACCTACTGCCAATGGAAGAGTTGACATCTCGACTGAAAGACCTCCCC ATGTCACCTGATCTGGAGTCACCAAGCCCCCAAGCCAGTTGCCAAGCTGCCATCTCAGATTTTAGTACAG ATCGTATTGACTTTTTTAGTGCCCTGGAGAAATTTGTAGAGCTTTCTCAAGAAACCCGGTCTCGGTCTTT TTCTCACTCAAGGATAGAAGAATTAGGTGGAGGAAGGAGTGAGGGCTGTCGTTTGTCAGTGATAGAAGTG GCAGCTTCAGAAATGGCAGCTGATGACCAGAGAAGCAGCTCTTTGAGTAATACGCCCCACGCCTCTGAAG AGTCTTCTGTGGATGAGGACCAGTCGAAGGCAATCACCGAACTTGTCAGCCCAGACATCATCATGCAATC TCACTCAGAAAATGCAATTTCAGTCAAAGAAATTGTGACTGAAATTGAGTCCATTAGTCAAGGAGTTGGG CAGGTTCAGTTAAAAGGAGACATCCTCTCTAACCCATGCCACACACCAAAGAAGAGCACCATTCATGAGC TGCCCCTGGAGAGGGTGCCAGCCCCTGAGAGCAAACCTGGACATTGGGAGCAGGATGAGAGTTTCTGTAG TGTCCAGCCAGAACTAGCTAGAGACTCAGGGAAGTGTGCCCCGGAGGAAGGCTGTCTGACCACACACTCA TCCACAGCAGACTTGGAAGAAGAAGAACCAGTTGAGGGGGAACATGACTGGGGCCCAGGGATGCACTCTG GTGCTAAGTGGTGCCCTGGGTCTGTGAGGCGAGCCACCCTGGAGTTTGAAGAGCGCTTGCGACAAGAGCA AGAGAACCATGGTACAGCCTCTGCAGGCCCCACATTATCCAATCGCAAAAACTCTAAGAATGATTCTTCT GTGGCAGACTTAATGCCAAAATGGAAAAGTGATGAAACTACCCCAGAACATTCATTTTTCCTCAAAGAAG CCGAGCCGAGCAAGGGTAAAGGGAAATGCAGTGGTTCTGAAGCCGGATCACTATCCCACTGTGAGCGTAA TCCCACCATGCCAGACTGTGAGCTGCTGGAGCATCATTCCTTGCCGGCTCCTCAGGACTGCCTAGGGTCA GATAGTAGAAGTAAGAAGCAGGAAGGAGACCTAAAGAAGCAGAGGGCTGTGGTTCCAAACCAGGAATGTG ACACACAGGCCATCCTTCTCCCCCTTCCCAAGAAAATAGAAATCATTGAGTATACGCCGACAGTTACATC ACTGGGTCACACCGAGCCAGGAGGTGAGGCGACACCCAGCAAAGAGGGTGAGAAACAAGGACTGAGGAAA GTGAAGATGGAGCAGTCTATCACTATGTTTTGTGCGCTGGATGAAAATCTGAACAGGACTCTGGAGCCCA GTCAAGTTTCTCTGCATCCCCAAGTGCTACCTCTGCCTCATTCTTCCTCAGAGTGTGACAGGCCCGCTGA CCCAAACCCTATGTTAAGTAGCCCGCAAGACAAAGGGGACTGCCCATCCACACCCTTCAAGACAGCAGCA CCTTTTGTCAGCTGCAGTACCCAGGGAGCATCTTTCAGCTTGGATTATTTGCTTCCCCACTCTGTGGTTC ACCTGGAAGGCTGCACAGAGCAAAGCAGTGCCACTGACAATGAACTGTCTCCAGAGCAGGCTAGCTGGGA GGACAGCCGGGGGCATTTCCTCTCCAGTGGTAGTGGAATGGCACACACATCCTCCCCATTAACTAACGAA GACCTAAGTTTAATTAACAAACTTGGTGACAGTGTTGGTGTGTTACAGAAAAAACTGGACCCGTCACCTG AAGCCTGTAGAATCCCACATAGCTCTAGTAGTGAAAATATAAGAGATCTCAGCCACAGCCGTGGTGTGGT GAAGGAGCATGCTAAAGAAATTGAGTCTCGAGTGATCTTCCAGGCAGGGTTTTCCAAAACATCCCAAATG AAGCGCTCAGCTTCCCTTGCCAAGTTGGGTTACCTGGACCTTTGTAAAGACTACTTACCAGATAGAGAGC TTGTCTCCTCAGAATCCCCTCATCTCAAATTGCTTCAGCCCTTCCTCAGAACAGACTCAGGCATGCATGC CTTGATGGCCCATGAGCCCTCAGAAAGCGCAGGTGCCCAGCAGAACCCACAGCCCACCAAGTATTCTGTA GAGCAACTCAAAACATCAGAGTGTATTGTGCAGAGCAAACCAGTGGAGAGGCCCAGTGTGCAGTATGCCA AAGAGTTTGGTTACAGTCAGCAGTGTTTGCTCCCCAAGGCAAGACCAGAATTGACTAGTTCTGAAGGAGG CCTTCCTTTGCTACAGACACAGGGTCTGCAGTACACAGGCCCCTCTCCAGGGCTGGCTGTGGCGCCCCGT CAGCAACATGGCAGAACTCACCCCCTTAGAAGACTGAAAAGAGCAAATGATAAAAAACGGACAACCAACC CCTTCTATAACACCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231800 representing NM_001291190 Red=Cloning site Green=Tags(s) MTLSTLAGERKALPASTCSLGGPDMIPYFSANAVISQNAINQLISESFLTVKGAALFLPRGNGSSTPRVS HRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDS TCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSALQSLHKACEVARMHNYYPGSLFLT WVSYYESHINSDQSSVNEWNAMQDVQSHRPDSPALFTDIPTERERTERLIKTKLREIMMQKDLENITSKE IRTELEMQMVCNLREFKEFIDNEMIVILGQMDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREI DNFFPGVFEYHNIRVYDEEATDLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWN LDRAYDYVKERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELNKKEM TTSADQIAEVKTVENLAAMPTVFMEHVVPQDANQKGLHTKERVICLEFSSQEFRAGQIEDELNLNDINGC SSGCCLSESKLPLDNCHASKALLQPGQAPDIANKFPDLAVEDLETDALKADMNVHLLPMEELTSRLKDLP MSPDLESPSPQASCQAAISDFSTDRIDFFSALEKFVELSQETRSRSFSHSRIEELGGGRSEGCRLSVIEV AASEMAADDQRSSSLSNTPHASEESSVDEDQSKAITELVSPDIIMQSHSENAISVKEIVTEIESISQGVG QVQLKGDILSNPCHTPKKSTIHELPLERVPAPESKPGHWEQDESFCSVQPELARDSGKCAPEEGCLTTHS STADLEEEEPVEGEHDWGPGMHSGAKWCPGSVRRATLEFEERLRQEQENHGTASAGPTLSNRKNSKNDSS VADLMPKWKSDETTPEHSFFLKEAEPSKGKGKCSGSEAGSLSHCERNPTMPDCELLEHHSLPAPQDCLGS DSRSKKQEGDLKKQRAVVPNQECDTQAILLPLPKKIEIIEYTPTVTSLGHTEPGGEATPSKEGEKQGLRK VKMEQSITMFCALDENLNRTLEPSQVSLHPQVLPLPHSSSECDRPADPNPMLSSPQDKGDCPSTPFKTAA PFVSCSTQGASFSLDYLLPHSVVHLEGCTEQSSATDNELSPEQASWEDSRGHFLSSGSGMAHTSSPLTNE DLSLINKLGDSVGVLQKKLDPSPEACRIPHSSSSENIRDLSHSRGVVKEHAKEIESRVIFQAGFSKTSQM KRSASLAKLGYLDLCKDYLPDRELVSSESPHLKLLQPFLRTDSGMHALMAHEPSESAGAQQNPQPTKYSV EQLKTSECIVQSKPVERPSVQYAKEFGYSQQCLLPKARPELTSSEGGLPLLQTQGLQYTGPSPGLAVAPR QQHGRTHPLRRLKRANDKKRTTNPFYNTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291190
ORF Size	4287 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291190.1, NP_001278119.1
RefSeq Size	9176 bp
RefSeq ORF	4290 bp
Locus ID	237860
UniProt ID	Q5SW75
MW	159.3 kDa
Gene Summary	Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.[UniProtKB/Swiss-Prot Function]

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