Asxl2 (NM_001270988) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4930556B16Rik; mKIAA1685
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231795 representing NM_001270988 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAAAAGGGCCGTAGGAAGAAGGGCAGGACCTGGGCGGAGGCCGCCAAGACGGTCTTAGAAAAAT ACCCTAATACACCCATGAGTCATAAAGAAATTCTTCAGGTTATCCAGAGAGAAGGACTGAAAGAAATCAG AAGTGGGACTTCCCCTCTTGCATGCTTGAATGCAATGCTGCACACAAACTCCCGAGGAGAAGAAGGCATC TTCTATAAAGTTCCAGGTAGAATGGGCGTGTATACTTTGAAGAAAGATGTGCCAGATGGAGTGAAAGAGC TGTCAGAAGGTTCAGAAGAAAGCAGTGATGGTCAGTCAGATTCTCAGAGCTCTGAGAACAGCAGCAGCAG TGATGGTGGCAGCAACAAAGAAGGGAGAAAGAGCAGGTGGAAAAGGAAAGTATCATCTAGACTGTCACAT CCACCCTCTCCCCCGTCAGGCTGCCCATCACCCACCATTCCAGCAAGTAAAGTCATTTCTCCATCACAGA AGCACAGCAAAAAGGCATTAAAACAGGCCTTGAAGCAGCAACAACAGAAGAAGAAGCAGCAGCAATGCAG ACCAAGCATGTCCATCTCCAATCAACACCTCTCTCTCAAGACTGTCAAAGCAGCCAGTGACTCTGTACCT GCCAAACCTGCCCTATGGGAAGGAAAGCAATCTGATGGACAGTCAAGCAGTCCCCAGAACTCAAATTCCA GCTTTTCTTCCTCAGTGAAAGTGGAAAGTTCTCTGCTAGGCTTGGGGAAGAAGTCTTTCCAGAGGTCTGA CAGACTCCACACAAGACAGATGAAAAGGACTAAATGTGCTGACATTGATGTTGAGACTCCTGATTCCATT CTGGTTAATACTAATCTTCGAGCACTGATCAACAAGCACACCTTTTCAGTTCTTCCTGGAGACTGCCAGC AGCGCCTGCTTTTGTTGCTTCCAGAGGTGGATCGACAGGTTGGTCCAGATGGCCTGATGAAGTTGAATGG CTCAGCCCTTAACAATGAGTTCTTCACTTCTGCAGCCCAGGGCTGGAAGGAAAGGCTCTCAGAAGGTGAA TTTACTCCTGAAATGCAGGTGAGAATTCGACAAGAGATTGAGAAGGAGAAGAAAGTAGAGCTGTGGAAGG AACAATTCTTTGAAAATTACTATGGTCAGAGTTCTGGCCTAAGCCTTGAAGATTCACAGAAACTGACAGC TTCCTCAAGTGATCCCAAAGCAAAGAAAACCCCAGCTGAGCAACCAAAATCCATACTTCCTTCAGAGGCC TCTCCTGTCAGAATTGTCCCAGTAGTTCCTCAGTCCGAGTGTAAAGAAGAAGCAGTGCAGATACCATCAC CATCCCAAAAGGAAGAAAATCAAGACGAGGCGAGGCCAGACTCCAAATCCCCAGAACCTGTTCTTGCATC AGCTAGCAATACAAATGAGCTTATCACCATGAAACCCATCAAGAGCCCTAAGGATGAGGGTCTCTTAGAG CAGAAGCCTGTTGCCTGTGCTGAACAAGAATCTGAGAAAGAAAATCATGTAACCACAACTTCTCGTAATA ACAAAAGTGAGAACCAAGAAGCGTTAGCTATATCCCCAAGCAAATCTAAAAATGCTGGGTTACAAAAACC CATAATAAAGCCTGTTGCAGAAGCCAGTCCACTGAACCCTGATATGAAGATGCCACCAGCAACTGTTACT GATCAGATCCAAGAAAGTCTCAAGAGGAAATCTTCTCTTACTGATGAAGAGGCCACCAGTAGCTGGGAGA AAAGACCACGTATCACTGAGAATCGCCAGCACCAGCAGCCATTTCAGGTCTCCCCACAGCCTTTTCTTAA TAGAGGGGACAGGGTCCAAGTGCGAAAAGTCCCACCTCTCAAGATCCCTGTCTCCAGAATCTCCCCGATG CTGTTTTCTACATCACAGGTCTCTCCCAGGGCTCGTTTTCCAATCTCCATCACTAGTCCTTACAGAACAG GAGCCAGAACTCTGGCAGACATCAAAGCAAAAGCCCAGTTGGTCAAAGCACAAAAGGCAGCAGCCGCAGC AGCTGCTGCAGCTGCTGCAGCCGCCTCAGTTGGAGGGACCATTCCGGGACCTGGCCCTGGGGGTGGACAA AGTCCAAGAGAGGGTGGTGAAAGGAAAATTGCTGGAGGAGGAAGTGCAGGCTCAGACCCAGTCAGTACAA ATGGAAAAGGTCCCACACTGGAACTGGCCGGAACTGGAAGCAGGGGAGGTACGAGAGAACTTTTACCCTG TGGTCCTCAACCTGAGACCAATATGCCAGGCCAAGCACAGCCTCCTGGTATCTCTGGAGCACAACTACAG CAAACCTCCTCAGTGCCTACGGGACTTGCCAGCAGTGGAGCATGCACAAGTGTCCCATTACCAGCCCACA TAGAAATATCAAACAGTGAAAAACCAAACCTCCACAAGGCAACAGCCACAGCAGCCTCTCCTTGCCACTT GCAAGACCCTAGGAGTTGCAGACTTGAAAAAGCTCTGTCTCCAACAGGGCCTCCTCTGATCTCAGGAGCC TCAACTGTTTATTTTGTAGCTGATGGCACAGTTGAGCCCAAAGCAGGTTCTAATAAGAATGCACCAAAGC CTTCAGCCTTAGCAAAGACAACTGCTCCTGCTCCACTAGATATGACTTCCTCTCCTGTGACAACAGCCAG TTTAGAAAAACTCCCTGTACCCCAGATCAGTGGAACTGCAACATCTACTGGATCAGCTCCATCCTCAAGC ACTTTGCCAGCAGCTTCTAGCCTTAAAACCCCAGGAACTTCTGCAAATATGAATGGACCCATTTCAAGAA CAAGTTCTAGTATCCCTGCTAATAATCCTTTAGTTACTCAGCTGCTCCAGGGCAAAGATGTTCCCTTGGA GCAAATTCTGCCTAAGCCTCTCACCAAAATTGAAATGAAAACAGTTCCACTGACTACAAAAGAGGAAAAA GGCATAGGGATATTCCCTGGTATCAGTGTAATGGAAAGTAGCAGCAGAGAGGAAGTTAATGGCAGGCAGG CCCATCTGGCTATCCCGCAGTTGGGGAAACCCTTGCAAAGTAAACAGCTTTCCCAGGTTCCAAGGCCAGT TTTCACTGCTAAGGACCGGAAGGACCCCTGCATTGACACTCACCAGTACCGAGAAGGTCTAAGTAAAACA ACGCAAGATCAGCTCTTTCAGACTCTTATCCAGAGGGCTCAGAGACAGAGTGTTCTTTCATTTGTACCGC CCTCTCAGTTCAACTTTGCTCACTCAGGTTTCCACTTGGAAGACATCTCCACAAGTCAGAAGTTCATGCT GGGTTTTGCTGGCAGAAGGACATCCAAGCCTGCAATGGCAGGTCACTATTTACTTAATATTTCCACCTAT GGCAGGGGTACAGAGAACATTAAAAGGACCCATTCTGTAAATCCTGATGATCGATTTTGTCTAAGTAGCC CCACTGAGGCGTTGAGAATGGGGCATGCAGATTATAAAAACACAACTGGAGAAATCAGCAGCAAAGAAGA TGAAAGTGATGAAGATCGCGTAGGTGATGAACAGGAGCCTATTTCAGTGAAGGAGGAGCCATGGGCTTCC CAGAGTTCTGGCAGGCATCCGCACCATGGGGAAGCTTCATCTACCAATGATTGTTTAGCTAGCAAGAATG GTAAGACTGAGGCACCAGTGAGTGAGCAAACCACTTTAGGCCAGGAGAATTACATATTCTCTAGAGGCCA AGCATCTGATGAAAAGAGCCTGCCTAGAGATTTTATTCCGGCAGCACATAAACAGATGACTCATGCTGTG AGAGGTAAGACAGTGTGTAGCAGCCCTGAGCTTTTCAATTCTACTGCTCTTTCTCTTCCCGCAGACAGTC CCACACATCAGCCTCTACTCCTTCCACCACTGCAAACCCCCAAATTGTATGGAAGCCCCACACAGATAGG GCCAAGCTATAGAGGCATGATCAATGTCTCCACCTCATCAGACATGGACCATAACTCTGCTATACCAGGC AGCCAGGTATCTAGCAATGTAGGGGACGTCATGTCGTTTTCAGTGACTGTCACTACCATCCCTGCCAGTC AAGCAATGAATCCCAGCAGCCATGGCCAGACAATTCCTGTTCAGACTTTTCCTGATGACAATAGTATCGA GGACACACCATCAAAATGTTACTGCCGGTTGAAAGCCATGATCATGTGCAAAGGATGTGGAGCTTTCTGC CATGATGATTGCATTGGCCCCTCCAAACTCTGTGTCTCCTGCCTTGTTGTTCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231795 representing NM_001270988 Red=Cloning site Green=Tags(s) MREKGRRKKGRTWAEAAKTVLEKYPNTPMSHKEILQVIQREGLKEIRSGTSPLACLNAMLHTNSRGEEGI FYKVPGRMGVYTLKKDVPDGVKELSEGSEESSDGQSDSQSSENSSSSDGGSNKEGRKSRWKRKVSSRLSH PPSPPSGCPSPTIPASKVISPSQKHSKKALKQALKQQQQKKKQQQCRPSMSISNQHLSLKTVKAASDSVP AKPALWEGKQSDGQSSSPQNSNSSFSSSVKVESSLLGLGKKSFQRSDRLHTRQMKRTKCADIDVETPDSI LVNTNLRALINKHTFSVLPGDCQQRLLLLLPEVDRQVGPDGLMKLNGSALNNEFFTSAAQGWKERLSEGE FTPEMQVRIRQEIEKEKKVELWKEQFFENYYGQSSGLSLEDSQKLTASSSDPKAKKTPAEQPKSILPSEA SPVRIVPVVPQSECKEEAVQIPSPSQKEENQDEARPDSKSPEPVLASASNTNELITMKPIKSPKDEGLLE QKPVACAEQESEKENHVTTTSRNNKSENQEALAISPSKSKNAGLQKPIIKPVAEASPLNPDMKMPPATVT DQIQESLKRKSSLTDEEATSSWEKRPRITENRQHQQPFQVSPQPFLNRGDRVQVRKVPPLKIPVSRISPM LFSTSQVSPRARFPISITSPYRTGARTLADIKAKAQLVKAQKAAAAAAAAAAAAASVGGTIPGPGPGGGQ SPREGGERKIAGGGSAGSDPVSTNGKGPTLELAGTGSRGGTRELLPCGPQPETNMPGQAQPPGISGAQLQ QTSSVPTGLASSGACTSVPLPAHIEISNSEKPNLHKATATAASPCHLQDPRSCRLEKALSPTGPPLISGA STVYFVADGTVEPKAGSNKNAPKPSALAKTTAPAPLDMTSSPVTTASLEKLPVPQISGTATSTGSAPSSS TLPAASSLKTPGTSANMNGPISRTSSSIPANNPLVTQLLQGKDVPLEQILPKPLTKIEMKTVPLTTKEEK GIGIFPGISVMESSSREEVNGRQAHLAIPQLGKPLQSKQLSQVPRPVFTAKDRKDPCIDTHQYREGLSKT TQDQLFQTLIQRAQRQSVLSFVPPSQFNFAHSGFHLEDISTSQKFMLGFAGRRTSKPAMAGHYLLNISTY GRGTENIKRTHSVNPDDRFCLSSPTEALRMGHADYKNTTGEISSKEDESDEDRVGDEQEPISVKEEPWAS QSSGRHPHHGEASSTNDCLASKNGKTEAPVSEQTTLGQENYIFSRGQASDEKSLPRDFIPAAHKQMTHAV RGKTVCSSPELFNSTALSLPADSPTHQPLLLPPLQTPKLYGSPTQIGPSYRGMINVSTSSDMDHNSAIPG SQVSSNVGDVMSFSVTVTTIPASQAMNPSSHGQTIPVQTFPDDNSIEDTPSKCYCRLKAMIMCKGCGAFC HDDCIGPSKLCVSCLVVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270988
ORF Size	4254 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270988.1, NP_001257917.1
RefSeq Size	9046 bp
RefSeq ORF	4257 bp
Locus ID	75302
UniProt ID	Q8BZ32
MW	152.8 kDa
Gene Summary	This gene encodes a homolog of the Drosophila Asx gene, which interacts with genes involved in axial patterning. Mice with mutations in this gene display abnormal patterning of the axial skeleton, suggesting a similar function in mice as in Drosophila. This gene may also be involved in bone mineral density, specifically osteoclastogenesis. [provided by RefSeq, Aug 2012]

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