Edc4 (NM_001301100) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC022641
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231792 representing NM_001301100 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCTGCGCGAGCATCGACATTGAGGACGCCACGCAGCATCTGCGGGACATACTCAAACTGGACC GGCCCGCCGGAGGTTCCAATGCCGAAAGCCAAAGGCCATCCAGTGCCTACAATGGAGACCTCAATGGGCT CCTGGTCCCCGACCCGCTCAGTTCAGGTGATGGTAACTCAACGAACAAACCTGGTATACGGACCATGCCA CCCATTAACCTGCAGGAAAAGCAGGTCATTTGCCTCTCTGGAGATGACAGCTCCACCTGCATTGGGATTT TAGCCAAGGAAGTGGAAATTGTGGCCAGCAGTGATTCTAGCATTTCCAGCAAGGCTCGGGGGAGCAACAA GGTGAAAATCCAGCCTGTCGCCAAGTATGACTGGGAGCAGAAATACTACTATGGCAACCTGATTGCAGTG TCTAACTCCTTCTTGGCATACGCCATTAGGGCTGCCAACAATGGCTCAGCAATGGTCCGTGTGATCAGTG TCAGCACTTCGGAGCGGACCCTGCTCAAAGGTTTCACAGGCAGTGTGGCTGATCTTGCTTTTGCACACCT CAACTCTCCACAGCTCGCCTGCCTGGATGAGGCTGGCAATCTTTTTGTGTGGCGCTTGGCTCTTGTTAAG GGTAAAATTCAAGAAGAGATTTTAGTCCATATCCGGCAGCCAGAGGGTACAGCGCTGAACCACTTCCGAA GGATCATCTGGTGCCCTTTCATCCCCGAGGAGAGTGAGGACTGTTGTGAGGAGAGCAGCCCGACAGTGGC CCTGCTGCATGAGGATCGGGCTGAGGTGTGGGACTTGGATATGCTTCGCTCCAGCCACAACACCTGGCCT GTGGATGTCAGCCAAATCAAGCAAGGCTTTATTGTGGTGAAAGGCCATAGCACGTGCCTAAGTGAAGGAG CCCTCTCCCCTGATGGGACTGTCCTGGCTACCGCAAGCCATGATGGCTTTGTCAAGTTCTGGCAGATCTA TATTGAAGGTCAGGATGAGCCAAGGTGTCTGCATGAATGGAAGCCTCATGATGGGAGGCCTCTTTCTTGC CTCCTGTTCTGTGATAACCATAAGAAACAGGATCCTGAGGTCCCGTTCTGGAGGTTCCTCATAACCGGCG CCGACCAGAATCGAGAGCTGAAGATGTGGTGCACAGTGTCCTGGACCTGCCTGCAAACCATTCGCTTCTC CCCAGATATCTTCAGCTCAGTGAGTGTGCCTCCCAGCCTCAAAGTTTGTTTGGACCTCTCAGCAGAGTAT TTGATTCTTAGTGATGTGCAACGAAAGGTCCTCTATGTGATGGAGCTGCTGCAGAACCAGGACGAGGGCC GCGCGTGCTTTAGTTCTATCTCTGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGCATCCAGGTTGT CAGTCGCTGCCGGCTACGGCACACTGAGGTCCTGCCTGCTGAGGAGGAGAATGACAGTCTGGGGACCGAG AGTTCCCATGGAGCTGGAGCCTTGGAATCCGCAGCTGGTGTGCTTATCAAGCTCTTTTGTGTGCACACTA AGGCACTACAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTCTCTAC CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGACCTGAACTAGGCTCCGAGGGGCTAGCTTCA GCTGCTCATGGTTCCCAGCCTGACCTGCGGCGCATTGTGGAACTGCCTGCTCCTGCAGACTTCCTCAGTC TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGATGCTTTCATGACACCTACTGCCTCCCTGCAGCA GATCTCTGCATCCCCTAGTAGTAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGC AGCTCTCTAACAGCTGTGTCTGCTGTGAGCAGCTCCTCAGCCATGGACCCTTCCTTGCCCAGACCACCTG AGGAGCTGACCTTGAGCCCCAAGCTGCAGCTAGATGGCAGCCTGACGCTAAACAGCAGCAGCAGCAGCCT GCAGGCAAGCCCTCGAAGCCTCCTTCCGGGCCTGCTCCCAGGCCCAGCTGACAAGTTGATTTCCAAGGGA CCTGGTCAGGTATCCACTGCTGCCTCTGCACTGTCCTTGGATTTGCAGGAAGTGGAGCCATTAGGGCTAC CCCAGGCCTCCCCAAGTCGAACCCGTTCTCCTGATGTGATCTCCTCAGCATCCACTGCCCTGTCCCAGGA CATTCCTGAAATCGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTCCTCTGTTCCTGAGGGCCTCATTGAG CCAGACAGCATGGCCTCAGCTGCCTCAGCACTACACCTACTCTCTCCTCGGCCCAGGCAAGGCCCTGAGC TTGGCTCTCAGCTGGGTCTGGATGGAGGCCCTGGAGATGGGGATAGGCATAGTACCCCTTCCCTGCTGGA AGCAGCCCTGACCCAGGAAGTTGCAACCCCTGACAGTCAAGTCTGGCCTACGGCACCTGACATTACTCGT GAGACCTGTAGCACACTGACAGAAAGCCCCAGGAATGGCCTCCAGGAAAAGCACAAAAGTCTGGCTTTCC ACAGGCCACCTTATCACTTGCTGCAGCAACGTGACAGTCAGGACACAAGTGCTGAGCAAAGTGACCATGA TGATGAGGTTGCCAGCCTTGCCTCTGCCTCAGGAGGTTTTGGCAGCAAAATTCCTACTCCACGGCTGCCT TCCAAGGATTGGAAGACGAAGGGATCCCCTCGGACGTCACCTAAGCTCAAGAGAAAAAGCAAGAAGGATG ATGGGGATTCAGCTGTGGGATCCCGGCTCACAGAGCACCAGGTGGCAGAGCCCCCTGAAGACTGGCCAGC ACTAATTTGGCAGCAGCAAAGAGAGCTGGCAGAGCTATGGCACAACCAAGAAGAACTGCTACAGCGTCTC TGTGCCCAGCTTGAAGGTCTTCAGAGTACTGTCACAGACCATGTAGAGCGTGCCCTGGAGACGCGGCATG AGCAGGAGCAACGGCGGCTGGAACGGGCACTGGCTGAGGGGCAGCAACGGGGTGGGCAGCTGCAGGAGCA GCTGACACAGCAGCTATCCCAGGCATTGTCTTCAGCTGTGGCTGGGCGGCTAGAGCGCAGCGTAAGGGAT GAAATCAAGAAAACGGTTCCTCCATGTGTCTCCAGAAGTCTGGAGCCTGTTGCAGGTCAACTAAGCAACT CAGTGGCTACCAAGCTTACAGCTGTGGAAGGCAGCATGAAAGAGAATATCTCTAAGCTACTCAAGTCCAA GAACTTAACAGATGCCATTGCCCGAGCAGCTGCAGACACGTTACAGGGACCAATGCAGGCTGCCTACCGG GAAGCCTTCCAGAGTGTGGTACTGCCAGCTTTTGAGAAGAGTTGCCAGGCTATGTTCCAGCAAATCAATG ACAGCTTTAGGCTGGGCACACAGGAATATTTGCAGCAGCTGGAGAGTCACATGAAGAGCCGGAAGGCCCG TGAACAAGAAGCTAGGGAGCCTGTGCTGGCCCAGCTTCGGGGCCTGGTCAGCACACTGCAGAGTGCCACC GAGCAGATGGCGGCCACTGTATCTAGCAGTGTTCGGGCCGAGGTGCAACACCAGCTGCATGTGGCTGTGG GCAGCTTGCAGGAGTCAATCTTAGCACAAGTACAGCGCATTGTCAAGGGTGAAGTGAGTGTGGCACTTAA GGAGCAGCAGGCCACCGTCACTTCCAGCATCATGCAGGCTATGCGTTCAGCTGCTGGCACACCTGTCCCC TCTGCCCACCTTGACTGCCAGGCCCAACAAGCCCATATCTTGCAGTTACTGCAGCAGGGCCACCTCAATC AGGCCTTCCAGCAGGCACTGACTGCTGCTGATCTCAATCTGGTGCTGTATGTGTGTGAAACTGTCGACCC AGCCCAGGTTTTTGGGCAGCCGCCCTGTCCACTATCCCAGCCTGTTCTCCTTTCCCTAATCCAGCAGCTA GCATCTGACCTTGGAACTAGAAGTGACCTCAAGCTCAGCTACTTGGAAGAAGCTGTGATGCACCTGGACC ACAGCGATCCCATCACTCGAGACCACATGGGCTCTGTCATGGCTCAGGTGCGCCAGAAGCTCTTCCAGTT CCTGCAGGCTGATCCACACAACTCACTTAGCAAAGCTGCCCGCCGCCTCAGCCTAATGTTACACGGCCTT GTGACCCCTAGCCTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231792 representing NM_001301100 Red=Cloning site Green=Tags(s) MASCASIDIEDATQHLRDILKLDRPAGGSNAESQRPSSAYNGDLNGLLVPDPLSSGDGNSTNKPGIRTMP PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVK GKIQEEILVHIRQPEGTALNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHNTWP VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGFVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC LLFCDNHKKQDPEVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY LILSDVQRKVLYVMELLQNQDEGRACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGTE SSHGAGALESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLSTHTAHEDFTFGESRPELGSEGLAS AAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPTASLQQISASPSSSSSSSSSSSSSSSSSS SSLTAVSAVSSSSAMDPSLPRPPEELTLSPKLQLDGSLTLNSSSSSLQASPRSLLPGLLPGPADKLISKG PGQVSTAASALSLDLQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSVPEGLIE PDSMASAASALHLLSPRPRQGPELGSQLGLDGGPGDGDRHSTPSLLEAALTQEVATPDSQVWPTAPDITR ETCSTLTESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDTSAEQSDHDDEVASLASASGGFGSKIPTPRLP SKDWKTKGSPRTSPKLKRKSKKDDGDSAVGSRLTEHQVAEPPEDWPALIWQQQRELAELWHNQEELLQRL CAQLEGLQSTVTDHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSVRD EIKKTVPPCVSRSLEPVAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYR EAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSAT EQMAATVSSSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQATVTSSIMQAMRSAAGTPVP SAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQL ASDLGTRSDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQADPHNSLSKAARRLSLMLHGL VTPSLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301100
ORF Size	4218 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301100.1, NP_001288029.1
RefSeq Size	4803 bp
RefSeq ORF	4221 bp
Locus ID	234699
UniProt ID	Q3UJB9
MW	152.9 kDa
Gene Summary	The protein encoded by this gene is thought to promote mRNA decay, and is known to interact with several mRNA decapping proteins. In humans, decreased expression of this gene prevents the accumulation of mRNA decapping proteins to mRNA processing bodies (P-body). Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.