Spag9 (NM_001199203) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3110018C07Rik; 4733401I23Rik; 4831406C20Rik; AW552012; Jip4; JLP; JSAP2; JSAP2a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231770 representing NM_001199203 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGAGGACGGTGTGGTATACCAGGAGGAGCCCGGAGGCTCCGGGGCCGTGATGTCAGAGCGGG TGTCCGGCCTGGCCGGCTCCATCTACCGCGAGTTCGAGCGGCTCATCGGGCGCTACGATGAGGAGGTGGT CAAGGAGCTGATGCCACTGGTGGTGGCCGTGCTGGAGAACCTGGACTCGGTGTTCGCGCAGGACCAGGAG CACCAGGTGGAGCTGGAGCTGCTGCGGGACGACAACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGG CGCTGCGCAAGCACGCCGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAAGGATTT ACAGACGCGAGTGGAGTCTCTAGAATCTCAAACAAGACAACTTGAACTCAAAGCAAAAAACTATGCTGAC CAGATCAGCAGACTTGAAGAGAGAGAAGCAGAACTGAAGAAGGAATATAATGCACTGCATCAGAGACACA CTGAGATGATCCATAATTATATGGAACACTTAGAAAGAACCAAACTTCATCAGCTCTCAGGGAGTGATCA ACTAGAGGCTACAGCTCATAGTAGAATTAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCT GCTGGAGATGGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAAT TTCAAGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGTCAGCCATAGCCCTGAACCTCTGAAGGC TGTAGAGCAGGAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGCTACCACTCCAGCTTCAACA GCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGATAATGAAGGATTTGTAAAAG GCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGGTTGCCCAAGAGACTAGGAA TGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAATCATTGAATCCACTCCTGAA TTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACCAAAGGCATAGAGAACAAAG CTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCTCGGGCCTTATTGGAGATGT GGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGAGAATCTCATACTGGAGAACACACAGCTG CTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTAATAGCCAAAGTGGATGAGCTGACCTGTG AGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAGCCAAGCTGAAGCTGGAGGACAAGAACAG AGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGAAGATGCAAGGCAAAAAGCAAAAGACGAT GATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACCAGAGTAGAAATGGCCCGAGTTCTCATGG AGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAGCTGTTCGATGGACGGAGATGATCCGGGC CTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAGCATCTGGCAATTTTTCAGCCGACTTTTT AGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTTAACCTGAAGTACAACGCCCCCACCTCTC ACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTCAGCTCCCTGGGGACAAGTCCAAGGCATT TGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCGCAGAGAGCAAAAGAGAGAGCAGTATCGG CAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAAGCTTTCGGCTGGAGTCTGCCTCAGAAGT ACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAAATTTACCTGTGCCTGTCTATCTCAGACC ACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGTAGGAGTCAATTTATCTGGTGGCAAGACA AGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAGGATATTGCTGGTTTGGACACTGAAGGCA GTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGGATCAAGAACTCAAGGAACAGCAGAAAGA ATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTGTACCAGCACCCACTCAACTACAAAGGTT ATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGTTTCACTGTTTGCAATTCTCATGTTCTGT GCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTGCAGGAGAAGAACTTTCTGAATCTGGTCA GGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAGCTCCGCAGAGATGGACAGCTTGCTGGGA GGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGAGCTGCCACTTCCCCAAGTACCAACGGTG CTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAAATAGCGAAGTTGATGAAAATATTCCAAC AGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTCCACTGAAGACACTGTGGACATCTCCCAG CCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGAGTTCAGATCCCAGAAGACCTCTCCCCAG TGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAATATCAGTATTGCCAAATGAACAAGACCT TGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAACCATGTGGCTTGGAGCTCAGAATGGCTGT TTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATTAAGCTCAAAGACTCAATTC TCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGGCGGATGGCACCCTTGCAATCTTCCACAG AGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTTAGACCTTGGACGTCCTCATCATTCCATA CGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAGGAACAAAATCTATGTGGTTCAACCAA AGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGAAGGAGAGCCAAGTACGGCAGCTTGCATG GGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCACGCTCCGCCTCTATCATGCACACACATAC CAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAAATGTTAGGTACTGGAAAACTGGGTTTCT CTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTTTGTGGGTGGGGACTGGAAATGGTGTCAT TATCTCCATCCCACTGACAGAAACAAATAAAACCTCAGGGACTCCAGGTAACCGTCCTGGAAGTGTAATC CGTGTATATGGTGATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATACCCTACTGTTCAATGGCAC ATGCACAGCTTTGTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAGTCCCAGGTCAGGTCAT TAGCCCACAAAGTAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGGGTCATCTGCACAGGAGCCC AGTAGCCAGACGCCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGCTACATTGACTTCCGGATGG GTGATGAAGGTGGAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAACCTTCAGTCACCAAAGCCGA GAGGAGTCACCTGATAGTGTGGCAAGTGATGTGTGGCAATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231770 representing NM_001199203 Red=Cloning site Green=Tags(s) MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQE HQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYAD QISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLEATAHSRIRKERPISLGIFPLP AGDGLLTPDTQKGGETPGSEQWKFQELSQPRSHTSLKVSHSPEPLKAVEQEDELSDISQGGSKATTPAST ANSDVSAIPPDTPSKEDNEGFVKGTDTSNKSEISKHIEVQVAQETRNVSTESGENEEKSEVQAIIESTPE LDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQL LETKNALNVVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEDKNRELEEELRKARAEAEDARQKAKDD DDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLF SSSSNATKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYR QVKAHVQKEDGRVQAFGWSLPQKYKQVANGQGETKMKNLPVPVYLRPLDEKDASMKLWCAVGVNLSGGKT RDGGSVVGASVFYKDIAGLDTEGSKQRSASQSSLDKLDQELKEQQKEFKNQEELSSQVWICTSTHSTTKV IIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEELSESGQVDKASLCGSMTSNSSAEMDSLLG GITVVGCSTEGLTGAATSPSTNGASPVIEKPPEMETENSEVDENIPTAEEATEATEGNAGSTEDTVDISQ PGVYTEHVFTDPLGVQIPEDLSPVFQSSNDSDVYKDQISVLPNEQDLAREEAQKMSSLLPTMWLGAQNGC LYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSI RCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTY QHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGTPGNRPGSVI RVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSGGADLTADKAGSSAQEP SSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMCGNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199203
ORF Size	3963 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199203.2
RefSeq Size	7392 bp
RefSeq ORF	3966 bp
Locus ID	70834
UniProt ID	Q58A65
MW	146.7 kDa
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function]

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