Arhgap33 (NM_001289682) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW324378; NOMA-GAP; Snx26; TCGAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231761 representing NM_001289682 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCAGGCACAGAAACAGTCAGATCCCATCCTGCCTTGGGGAGCTTCATGGGCTGGCAGGGGACAGA CTCTGAGGGCCCGCAGCACTGACAGCCTGGATGGCCCAGGGGAGGGCTCAGTGCAGCCCGTACCCACTAC TGGGGGGCCGGGTACAAAGGGGAAGCCTGGGAAGAGGCTCTCAGCTCCTCGAGGCCCTTTCCCCCGGCTG GCTGACTGTGCCCATTTCCACTATGAGAATGTTGACTTTGGCCACATTCAGCTCCTGCTGTCTCCAGAGC GTGAGGGCCCCAGCCTCTCTGGAGAAAATGAGCTGGTCTTTGGGGTACAGGTGACCTGTCAGGGCCGCTC CTGGCCAGTCCTCCGGAGCTATGACGATTTCCGTTCTCTGGATGCCCATCTCCACCGGTGCATATTTGAC CGGAGATTTTCCTGCCTCCCAGAGCTCCCTCCACCTCCAGAGGGTACCAGGGCTGCCCAGATGCTGGTAC CGCTGCTGCTGCAGTACCTGGAGACCCTGTCAGGACTGGTGGACAGTAACCTCAACTGTGGACCTGTGCT CACTTGGATGGAGCTGGACAACCATGGCCGGCGCCTGCTCCTCAGTGAGGAAGCCTCCCTCAATATCCCT GCTGTGGCTGCTGCCCACGTGGTCAAGCGGTACACTGCCCAGGCGCCAGATGAGCTGTCCTTTGAGGTGG GAGACATTGTCTCAGTGATCGACATGCCACCTACAGAGGATCGGAGCTGGTGGCGGGGCAAACGGGGCTT CCAGGTTGGTTTCTTCCCCAGTGAGTGTGTGGAACTCTTCACGGAGAGGCCAGGGCCTGGCCTAAAGGCA GATGCTGACAGTCCCCTGTGTGGCATCCCAGCTCCCCAGGGCATCTCTTCTCTGACCTCAGCTGTGCCCC GGCCACGTGGGAAGCTGGCAGGACTCCTCCGCACCTTCATGCGCTCTCGTCCTTCTCGCCAGCGGCTGCG CCAACGGGGAATCCTGCGGCAGAGGGTGTTTGGTTGTGATCTTGGAGAGCACCTCAGCAACTCAGGCCAA GACGTGCCCCAAGTGCTGCGCTGCTGCTCTGAGTTTATTGAGGCCCATGGTGTGGTGGATGGAATCTACA GGCTCTCTGGCGTGTCCTCCAACATCCAGAGGCTTCGGCATGAGTTTGATAGTGAGAGGATCCCTGAGCT GTCTGGCCCCGCCTTCCTACAAGATATCCACAGTGTGTCCTCCCTCTGCAAGCTCTACTTCCGGGAACTG CCCAACCCCTTGCTCACCTACCAGCTCTATGGGAAGTTCAGTGAGGCTATGTCAGTGCCTGGGGAGGAAG AACGCCTAGTGCGAGTGCATGACGTCATCCAGCAGCTGCCTCCACCACACTACAGGACCCTGGAGTACCT GCTGAGGCACCTGGCCCGCATGGCAAGACACAGCGCTAACACCAGCATGCATGCCCGCAACCTGGCCATT GTCTGGGCACCCAACCTGCTACGGTCTATGGAACTGGAGTCGGTGGGGCTGGGTGGGGCAGCCGCCTTCC GGGAGGTCCGGGTGCAGTCGGTGGTGGTGGAGTTCCTGCTTACCCACGTGGAGGTTCTGTTCAGCGATAC GTTCACCTCTGCTGGCCTGGACCCTGCAGGTCGCTGCCTCCTTCCCAGACCCAAGTCCCTTGCGGGGAGC AGTCCCTCCACTCGCCTGCTAACACTGGAAGAAGCCCAGGCACGAACTCAGGGCCGTCTTGGAACACCTA CAGAGCCCACGACTCCTAAGACGCCAGCCTCACCAGTGGAAAGGAGGAAAAGAGAGAGGGCGGAGAAACA AAGGAAGCCAGGAGGTAGCAGCTGGAAGACATTCTTTGCTCTGGGGCGGGGCCCCAGCATACCCCGGAAG AAGCCGCTGCCATGGCTGGGTGGCAGCCGAGCCCCACCACAGCCTTCAGGCAGCAGACCTGACACTGTCA CGCTGAGATCTGCCAAAAGTGAGGAGTCTCTGTCATCCCAGGCCAGCGGGGCTGGCCTCCAGAGGCTGCA CCGGCTGCGACGACCCCACTCCAGCAGCGATGCTTTTCCCGTGGGCCCAGCACCTGCTGGCTCTTGCGAG AGCTTGTCCTCTTCTTCTTCCTCTTCTTCCTCTTCCTCCTCCTCCTCCTCCTCAGAGTCTTCAGCAGGTG GCCTGGGGCCACTCTCTGGCTCCCCCTCACACCGCACCTCAGCCTGGCTAGATGATGGCGATGATCTGGA TTTCAGTCCACCCCGCTGTCTGGAAGGACTTCGGGGACTTGACTTTGATCCCCTTACCTTTCGCTGCAGC AGCCCCACCCCAGGGGACCCTGCACCTCCTGCCAGCCCAGCACCTCCAGCCTCAGCCTCTGCCTTCCCAC CTCGGGCAACCCCACAGGCTCTGTCACCCCATGGACCCACTAAGCCTGCTTCACCCACTGCCCTGGACAT CTCAGAGCCCCTGGCTGTATCAGTACCACCTGCTGTCCTGGAACTACTGGGAGCTGGAGGAACGCCTGCC TCAGCCACCCCAACACCGGCTCTCAGCCCTCATCTCATCCCCCTGCTGCTGCGTGGAGCTGAGGCCCAGC TAAGTGACACCTGTCAGCAGGAGATCAGCAGCAAGCTAGCACCAACCCGGGGAGCTCCAGGCCAGCAAAG TCCTGGCGGCATGGATTCACCGTTATTGCCCCCACCCTTGCCTCTGCTGCGCCCTGGTGGGGCTCCGCCC CCTCCCCCCAAGAACCCAGCTCGTCTCATGGCCCTGGCCTTGGCTGAGCGAGCTCAGCAGGTAGCAGAGC AACAGAGCCAACAAGAGCAGGGGGGCACCCCACCTGCTCCCCACTCCCCCTTTCGACGCTCTCTGTCCCT AGAGGTGGGTGGGGAGCCTGTGGGGACTTCAGGGAGTGGGATACACCCTCCCTCCTTAGCTCACCCAGGT GCCTGGGCTCCAGGTCCCCCACCTTACCTCCCAAGGCAACAGAGTGATGGGAGCCTGGTAAGAAGCCAGC GGCCCTTGGGGACCTCAAGGAGAAGTCCCAGAGGGCCTTCCCAGGTCAGTGCCCATCTCAGGGCAAGCGG GGCTTACAGGGATGCTCCAGAGATGGCAGCCCAGTCACCATGTTCTGTCCCCTCACAGGGTTCTAACCCC AGCTTCTTCTCAACCCCCCGAGAGTGTTTGCCACCTTTCCTTGGCGTCCCCAAACAAGGCTTGTACTCTC TGGGACCCCCATCCTTCCCACCTAGCTCTCCAGCCCCAGTCTGGAGGAACTCTCTGGGTGCCCCCTCAGC ATTGGACAGGGGAGAGAATCTGTACTATGAAATTGGGGTAGGGGAGGGGACCTCCTACTCAGGCCCCAGC CGGTCCTGGAGTCCGTTTCGCTCCATGCCTCCCGACAGGCATAATGCCTCCTATGGTATGCTTGGCCAGT CACCACCGCTCCACAGGTCCCCTGATTTCCTGCTCAGCTACCCGCCTCCCCCCTCCTGCTTTCCCCCAGA GCACCTTACCCACTCAGTTTCACAGCGCCTTGCCCGGCGCCCTACCCGTCCTGAGCCCCTCTATGTCAAC CTAGCCCTAGGGCCCAGGGGCCCTTCACCTGCCTCTTCTTCCTCTTCCTCTCCTCCTGCCCACCCCAGGA GCCGGTCAGATCCTGGGCCTCCTGTTCCCCGCCTCCCTCAGAAACAGCGGGCTCCTTGGGGTCCACATAC TCCCCATCGGGTGCCTGGACCTTGGGGTTCCCCCGAACCTTTTCTGCTCTATAGGCCAGCTCCACCATCC TATGGGAGGGGAGGGGAGGTCCGAGGATCCTTGTACAGAAATGGAGGACATAGAGGAGAGGGGGCTGGTC CCCCTCCTCCTTACCCCACACCCAGCTGGTCCTTACATTCAGAAGGACAGACCAGAAGTTACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231761 representing NM_001289682 Red=Cloning site Green=Tags(s) MLQAQKQSDPILPWGASWAGRGQTLRARSTDSLDGPGEGSVQPVPTTGGPGTKGKPGKRLSAPRGPFPRL ADCAHFHYENVDFGHIQLLLSPEREGPSLSGENELVFGVQVTCQGRSWPVLRSYDDFRSLDAHLHRCIFD RRFSCLPELPPPPEGTRAAQMLVPLLLQYLETLSGLVDSNLNCGPVLTWMELDNHGRRLLLSEEASLNIP AVAAAHVVKRYTAQAPDELSFEVGDIVSVIDMPPTEDRSWWRGKRGFQVGFFPSECVELFTERPGPGLKA DADSPLCGIPAPQGISSLTSAVPRPRGKLAGLLRTFMRSRPSRQRLRQRGILRQRVFGCDLGEHLSNSGQ DVPQVLRCCSEFIEAHGVVDGIYRLSGVSSNIQRLRHEFDSERIPELSGPAFLQDIHSVSSLCKLYFREL PNPLLTYQLYGKFSEAMSVPGEEERLVRVHDVIQQLPPPHYRTLEYLLRHLARMARHSANTSMHARNLAI VWAPNLLRSMELESVGLGGAAAFREVRVQSVVVEFLLTHVEVLFSDTFTSAGLDPAGRCLLPRPKSLAGS SPSTRLLTLEEAQARTQGRLGTPTEPTTPKTPASPVERRKRERAEKQRKPGGSSWKTFFALGRGPSIPRK KPLPWLGGSRAPPQPSGSRPDTVTLRSAKSEESLSSQASGAGLQRLHRLRRPHSSSDAFPVGPAPAGSCE SLSSSSSSSSSSSSSSSSESSAGGLGPLSGSPSHRTSAWLDDGDDLDFSPPRCLEGLRGLDFDPLTFRCS SPTPGDPAPPASPAPPASASAFPPRATPQALSPHGPTKPASPTALDISEPLAVSVPPAVLELLGAGGTPA SATPTPALSPHLIPLLLRGAEAQLSDTCQQEISSKLAPTRGAPGQQSPGGMDSPLLPPPLPLLRPGGAPP PPPKNPARLMALALAERAQQVAEQQSQQEQGGTPPAPHSPFRRSLSLEVGGEPVGTSGSGIHPPSLAHPG AWAPGPPPYLPRQQSDGSLVRSQRPLGTSRRSPRGPSQVSAHLRASGAYRDAPEMAAQSPCSVPSQGSNP SFFSTPRECLPPFLGVPKQGLYSLGPPSFPPSSPAPVWRNSLGAPSALDRGENLYYEIGVGEGTSYSGPS RSWSPFRSMPPDRHNASYGMLGQSPPLHRSPDFLLSYPPPPSCFPPEHLTHSVSQRLARRPTRPEPLYVN LALGPRGPSPASSSSSSPPAHPRSRSDPGPPVPRLPQKQRAPWGPHTPHRVPGPWGSPEPFLLYRPAPPS YGRGGEVRGSLYRNGGHRGEGAGPPPPYPTPSWSLHSEGQTRSYC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289682
ORF Size	3915 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289682.1, NP_001276611.1
RefSeq Size	4439 bp
RefSeq ORF	3918 bp
Locus ID	233071
UniProt ID	Q80YF9
MW	139.8 kDa
Gene Summary	May be involved in several stages of intracellular trafficking (By similarity). Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.[UniProtKB/Swiss-Prot Function]

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