Pex1 (NM_001293806) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5430414H02Rik; E330005K07Rik; ZWS1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231753 representing NM_001293806 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGTGGAGCAGCGATCGCTTAGCGGGTGCGGGGTCTGGTGGGGCGGTCGTGACGGTGGCCTTCACGAATG CTCGGGACTGCTTCCTGCATCTGCCGCGGCGCCTCGTGGCCCAGCTTCACCTGCTGCAGAATCAAGCTAT AGAAGTGGCCAGCGATCACCAGCCTACCTACCTGAGCTGGGTGGAAGGCAGGCATTTTAATGATCAAAGT GAAAATGTGGCAGAAATTAATAGACAAGTTGGCCAGAAACTTGGACTCTCAAGCGGAGATCAGGTGTTTC TCAGGCCTTGTTCCCATGTGGTATCTTGCCAACAGGTTGAAGTGGAGCCTCTCTCAGCAGATGACTGGGA AATACTGGAGCTGCACGCCATTTCCCTTGAACAGCATCTTCTGGATCAGATTCGAATAGTTTTCCCCAAA GCTGTTGTTCCCATCTGGGTTGACCAGCAGACCTACATATTTATCCAGATCGTCACTCTGATGCCAGCTG CCCCTTATGGAAGGCTAGAAACTAACACCAAACTCCTTATTCAGCCAAAGACACGCCAAGCCAAAGAGAG CACATTTCCAAAAGAAGGAGATGCACACGGACAAGTTCATAGTTATGGGCGAGAACAGAAAGGATTGTCA AAGGAATTACAAACCAGGCAGCTTCATACGAACTCAGAGGGTATCACTGCATCCAATGGAAGAGACCCAA AAGTCCCAGGTGGCCCGTTGAAGCCAAGCTGGTGGGCTGTGCTCGGAAGCATGCTTTCCTTTGGGCCTGA CAGTAAACAGGAGTCAGCCTGGGGCTCGTTGGAACTCGGTGCTTTCAAAAACATGCAGTCACAGGCTGCC CCTCTGGAAGGTACTTTCAGAGTATGCCAAGTCCAGCCACCTAGTGCGCGTACCACCACCGCTACCTCTG TGTTTCACAAACACTGCACCGCCCACGTATTTCCTTGGGACCAAGAATACTTTGATGTGGAGCCCAGCTT TACTGTGACCTATGGAAAACTAGTTAAGCTACATTCTCCAAAACAACAGCAAGACAAAAGTAAGCAGGGT GTCCTGTTGCCTGACAAAGAGAAGCAGCTGTCCAAGTCTCCAGATCATAAGCAGATCAGCTCCAACCGCA GTGAGGAGGCTGCTGAGGCCTGTGTGCTGAAGGTAGTCTGGAATGGACTCGAGGAGTTGAAGAATGCCAC AGAGTTCACCGAAAGTCTAGAGCTTCTCCACCGTGGGAAAGTCTGGATTCCAGATGACCTGAGAAAGAGA CTAAATATAGAAATGCATGCAGTAGTCAGAATAACTCCACTGGAAACCACGCCTAAAATCCCAAGATCTC TGAAATTACAACCTAGAGAGAACTTACCTAAAGACGTCAATGAAGAAACTATAAAAACAGTGTTCTCATC TTGGGTACAGCAGTCCGCTACCACGATGCTTCCTTTGGTAATATCAAAGGAAGAGCGTATTAAGCTGGAA ATTAAAGATGGGCTGAGAGAGTTCTCTCTGAGTACAGTTCATTCTCAGGAAAAAGAAAAGGAAGAAGGAA AAACTGTGTTTGTGTTGAGTTCCATCCTGCTGCAGAAGATCTCAGTACAAGTCCTTCTAGAGCCCATGAT AAAAGAAGAACAGAGTGCGGAAATCGACTTTCTTCTTCCCTCTTTAACGCTGAGCTCTTTGGGGGGAGTG AGTGCCTTAGGTGCATCTGCAATGGAGCACATCACTCACAGTCTCCTGGGACGCCCGTTGTCTAGGCAGC TGATGGCCCTCGTTGCAGGACTTAGGAATGGCGCTCTTTTGATCACTGGAGGAAAGGGAAGCGGGAAGTC AACATTCGCGAAAGCCATCTGCAAGGAAGCACAGGACACTCTGGATGCCCGTGTGGAGACAGTCGACTGC AAGGCTTTACGAGGAAAAAGGCTTGAAAGCATACAAAAAGCGCTAGAGGTGGCTTTCTCAGAGGCCGCCT GGAGGCAGCCATCTGTCATTCTGCTGGATGACCTGGACCTCATTGCCGGACTGCCAAGTGTCCCCGAGCA GGAGCACAGCCCTGAAGCGGTGCAGAGCCAGCGGCTTGCACATGCTTTGAACGATATGATCAAAGAGTTT GTTTCCACGGGAAGCTTGGTGGCACTCATCGCCACGAGCCAGCTCCAGCAGTCTCTGCACCCTTCCCTTG TGTCTGCTCAAGGAATCCACACGTTTCAATGTGTCCAGCACCTTCAGCCTCCCAATCCGGAACAGAGATG TGAAATTCTGCACAGTGTTGTGAAGAATAAACTGGGCTGTGATATAAGCAATTTCCCTGACTTGGACCTG CAGTGCATAGCTAAAGACACAGAAGCGTTTGTGGCTCGTGACTTTACAGTTCTTGTGGACCGAGCCATAC ACTCTTCTCTCTCTCGCCAGCATAGCTCCTCTAGGGAAGACTTGACTTTAACAACATCAGACTTCCAAAA GGCTCTCCGTGGATTCCTTCCTGCTTCTCTGCGAAATGTCAACTTGCATAAACCTAGAGACCTGGGCTGG GACAAGATTGGTGGATTACATGAAGTTCGGCAGATCCTCATGGATACTATCCAGTTACCAGCCAAGTACC CAGAATTATTTGCAAACTTACCCATACGACAGAGGACAGGAATACTGCTTTATGGTCCTCCAGGGACAGG AAAAACTTTACTTGCTGGGGTAGTTGCAAGAGAGAGTGGAATGAATTTTATTAGTATTAAGGGACCAGAG TTACTCAGCAAATATATTGGCGCAAGTGAGCAAGCTGTTCGAGATGTTTTCATCAGAGCACAGGCTGCAA AGCCCTGCATTCTTTTCTTTGATGAGTTTGAGTCCATCGCTCCTCGAAGAGGCCATGACAACACAGGGGT TACAGACCGAGTAGTCAACCAGTTGCTGACACAGTTAGACGGAGTAGAAGGCTTACAGGGAGTTTATGTG CTGGCTGCTACTAGTCGCCCTGACTTGATCGACCCTGCCCTGTTGCGGCCTGGCAGACTGGATAAATGTG TATACTGCCCTCCTCCAGATCAGGTGTCCCGTCTTGAGATTTTAACTGTCCTCAGCAAGTCTCTAGCTCT GGCAGATGACGTGGACCTTCAGCACGTGGCGTCGGTCACCGACTCGTTCACTGGAGCGGATCTGAAAGCT CTGCTGTACAACGCTCAGCTGGAGGCCTTGCAGGGACGGCTGCTGCCCAGTGGGCTTCCCGATGGAGGCT CCAGCTCTGACAGTGACCTGAGTCTGTCTTCAATGGTCTTTCTTAACCACAGCAGTGGTTCCGACGACTC CGCTGGAGATGGAGAATGTGGCTTAGAGCAATCCCTGCTTTCTCTCGAGATGTCTGAGATCCTTCCAGAC GAATCAAAATTCAATATGTACCGGCTCTACTTTGGAAGCTCGTATGAATCGGAGCTTGGAAATGGGACCC CTTCTGACTTGAGCTCACACTGTCTGTCTGCACCAAGCTCCGTGACTCAGGATTTACCTGCAGCTCCTGG GAAAGACCCGTTATTTACACAACATCCTGTGTTCAGGACACCTTCCCAAGAAGGCTGCCAAGACCTCACC CAGGAGCAGAGAGATCAGCTGAGGGCAGAGATCAGCATCATCAAAGGCAGATACCGGAGCCAAAGTGGAG AGGATGAATCCCTTAACCAGCCTGGACCAATCAAAACCACTTTTGCTATTAGCCAGGCACATTTAATGAC TGCACTTGCCCACACAAGACCGTCTATTAGTGAAGATGAAGGGAAGGAATTTGCTGAGCTGTATGAGAAC TTTCAAAATCCAAAGAAGAGAAAAAATCAAAGTGGAACAGTGTTTCGAACTGGACAGAAAGTAACTTTAG CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231753 representing NM_001293806 Red=Cloning site Green=Tags(s) MWSSDRLAGAGSGGAVVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVASDHQPTYLSWVEGRHFNDQS ENVAEINRQVGQKLGLSSGDQVFLRPCSHVVSCQQVEVEPLSADDWEILELHAISLEQHLLDQIRIVFPK AVVPIWVDQQTYIFIQIVTLMPAAPYGRLETNTKLLIQPKTRQAKESTFPKEGDAHGQVHSYGREQKGLS KELQTRQLHTNSEGITASNGRDPKVPGGPLKPSWWAVLGSMLSFGPDSKQESAWGSLELGAFKNMQSQAA PLEGTFRVCQVQPPSARTTTATSVFHKHCTAHVFPWDQEYFDVEPSFTVTYGKLVKLHSPKQQQDKSKQG VLLPDKEKQLSKSPDHKQISSNRSEEAAEACVLKVVWNGLEELKNATEFTESLELLHRGKVWIPDDLRKR LNIEMHAVVRITPLETTPKIPRSLKLQPRENLPKDVNEETIKTVFSSWVQQSATTMLPLVISKEERIKLE IKDGLREFSLSTVHSQEKEKEEGKTVFVLSSILLQKISVQVLLEPMIKEEQSAEIDFLLPSLTLSSLGGV SALGASAMEHITHSLLGRPLSRQLMALVAGLRNGALLITGGKGSGKSTFAKAICKEAQDTLDARVETVDC KALRGKRLESIQKALEVAFSEAAWRQPSVILLDDLDLIAGLPSVPEQEHSPEAVQSQRLAHALNDMIKEF VSTGSLVALIATSQLQQSLHPSLVSAQGIHTFQCVQHLQPPNPEQRCEILHSVVKNKLGCDISNFPDLDL QCIAKDTEAFVARDFTVLVDRAIHSSLSRQHSSSREDLTLTTSDFQKALRGFLPASLRNVNLHKPRDLGW DKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVVARESGMNFISIKGPE LLSKYIGASEQAVRDVFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYV LAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILTVLSKSLALADDVDLQHVASVTDSFTGADLKA LLYNAQLEALQGRLLPSGLPDGGSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLEQSLLSLEMSEILPD ESKFNMYRLYFGSSYESELGNGTPSDLSSHCLSAPSSVTQDLPAAPGKDPLFTQHPVFRTPSQEGCQDLT QEQRDQLRAEISIIKGRYRSQSGEDESLNQPGPIKTTFAISQAHLMTALAHTRPSISEDEGKEFAELYEN FQNPKKRKNQSGTVFRTGQKVTLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293806
ORF Size	3852 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293806.1, NP_001280735.1
RefSeq Size	4553 bp
RefSeq ORF	3855 bp
Locus ID	71382
UniProt ID	Q5BL07
MW	141.9 kDa
Gene Summary	Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes (By similarity).[UniProtKB/Swiss-Prot Function]

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