Frmpd4 (NM_001290428) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm196; Pdzd10; Pdzk10; PKAP1; preso; Preso1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231749 representing NM_001290428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACAAACCGAGATGGACGTGACTACTTCATCAATCACATGACACAGGCAATCCCATTTGATGACC CTCGGTTTGACAGCTGCCAAATCATCCCCCCAGCTCCACGAAAGGTGGAGATGAGGAGAGACCCTGTGCT GGGCTTTGGGTTTGTGGCAGGGAGCGAAAAGCCAGTGGTCGTTCGGTCAGTAACACCAGGTGGCCCCTCA GAAGGCAAGCTGATCCCAGGAGATCAGATTGTAATGATTAATGATGAAGCAGTCAGCGCTGCACCAAGAG AGAGAGTCATCGACCTGGTCAGGAGCTGCAAAGAATCGATTCTGCTCACTGTCATTCAGCCCTATCCATC TCCCAAATCAGCATTTATCAGTGCTGCTAAAAAGGCAAGATTGAAGTCCAATCCAGTCAAAGTGCGCTTT TCCGAAGAGGTCATAATCAATGGCCAAGTGTCGGAAACTGTTAAAGACAATTCACTTCTTTTTATGCCAA ATGTTTTGAAAGTCTATTTGGAAAACGGACAGACCAAATCCTTTCGTTTTGACTGCAGCACCTCCATCAA GGATGTCATCTTAACCCTGCAAGAGAAGTTGTCTATCAAAGGCATTGAACACTTCTCCCTCATGCTGGAG CAGAGAATTGAAGGGGCTGGCACCAAGCTGCTCTTGCTTCATGAACAGGAGACACTCACTCAGGTGACAC AGAGGCCCAGCTCCCATAAGATGAGGTGTCTTTTCCGAATCAGTTTTGTTCCCAAAGATCCCATTGACCT GTTAAGAAGAGATCCAGTTGCTTTTGAGTATCTCTATGTTCAGAGTTGTAACGATGTCGTTCAGGAGCGA TTTGGACCAGAGCTGAAATATGACATTGCCTTGCGGCTGGCCGCGTTACAAATGTACATTGCTACTGTCA CCACCAAACAGACGCAGAAAATCTCCCTCAAATACATTGAGAAAGAATGGGGACTAGAGACTTTTCTTCC ATCTGCTGTGCTGCAGAGCATGAAAGAGAAGAACATCAAGAAAGCACTCTCCCACCTTGTCAAAGCAAAT CAAAACTTGGTACCACCGGGTAAAAAGCTCTCTGCACTACAAGCTAAGGTCCACTATCTCAAGTTCCTCA GCGACCTGCGACTATATGGGGGCCGTGTATTCAAGGCAACATTAGTGCAGGCAGAGAAGCGCTCAGAAGT AACTCTTCTGGTTGGTCCCCGGTATGGCATAAGCCATGTCATAAACACCAAAACCAATCTGGTGGCTCTT TTAGCTGACTTCAGCCATGTCAACAGGATTGAAATGTTTACTGAAGAGGAGAGCTTGGTGAGGGTGGAGT TGCATGTGCTAGATGTGAAGCCCATTACACTACTTATGGAGTCATCAGACGCCATGAACCTGGCCTGTCT GACAGCTGGATACTACCGGCTGCTTGTGGACTCCAGGAGGTCAATATTTAACATGGCCAACAAGAAAAAC GCAGGCACGCAGGACACAGGATCAGAAAATAAAGGCAAGCATAATCTCCTTGGTCCTGATTGGAACTGTA TGCCCCAGATGACCACCTTCATTGGTGAAGGCGAACAAGAAGCCCAAATCACATATATAGATTCTAAGCA GAAGACAGTTGAGATGACAGACAGCACCTTGTGTCCCAAAGAGCACCGGCACTTATATATCGACAACTCA TACAGTTCAGATGAACTTAACCAGCCGCTGACTCAGCCAGGTGATGCCCCCTGTGAGGCCGACTATAGAA GTCTAGCTCAGCGGTCCCTTTTGACCCTCTCAGGACCAGACACTCTGAAGAAAGCACAGGAATCTCCGCG AGGAGCTAAAGTGTCCTTTATTTTTGGAGATCTTGCCTTAGATGATGGCATGAGTCCCCCAACTATAGGC TATGAAAGAATGTTAGAGGAGAATCCAGAAATGCTGGAGAAGCAGAGGAATCTCTACATCAGCAGTGCCA ATGACATGAAGAACCTGGACCTCACTCCAGACACAGACAGCATCCAGTTTGTGGCAAATTCTGTATATGC AAACATAGGTGATGTGAAGAACTTTGAGGCCCCTGAGGGGATAGAGGAGCCCCTCTTACATGACATCTGT TATGCAGAAAATACGGACGATGCAGAAGATGAAGATGAGGTGAGCTGCGAGGAGGACCTCGTGGTGGGTG AAATGAACCAACCAGCCATCCTTGACCTGTCCGGGTCAAGTGATGACATTATTGACCTCACAACCCTGCC TCCTCCAGAAGGAGATGACAATGAGGATGACTTCCTCCTGCGTTCTCTGAACATGGCCATCGCTGCTCCC CCACCTGGCTTTAGAGACAGTTCTGATGAAGAGGACACTCAGAGCCAGGCAACGTCCTTCCATGAGGACA AAGAACAAGGCAGCAGCCTGCAGAATGAGGAGATCCCTGTGTCCCTCATTGATGCTGTGCCCACCAGTGC AGAGGGCAAGTGTGAGAAGGGACTGGACCCTGCCGTCGTTTCCACACTGGAAGCCCTAGAAGCTCTTTCA GAAGAACAGCAGAAGAGTGAAAATTCAGGTGTAGCCATCTTGCGAGCTTATAGTCCCGAGTCTTCATCAG ACTCGGGCAATGAGACTAACTCTTCTGAAATGACAGAGGGTTCTGAGCTAGCTGCAGCACAGAAGCAGTC GGAAAGCCTCTCCCGCATGTTCTTGGCCACTCATGAAGGCTATCACCCTCTGGCAGAAGAGCAGACAGAG TTCCCCACCTCCAAAGCCCCCTCAGTGGGCTTGCCTCCAAAGTCCTCCCATGGCCTGGCTGCTCGCCCAG CGACCGACCTCCCACCCAAAGTTGTGCCTTCCAAGCAGATCCTTCACTCAGACCACATGGAAATGGAGCC AGAAACCATGGAGACCAAGTCTGTCACTGACTATTTTAGCAAACTGCATATGGGGTCAGTAGCATATTCC TGTACCAGCAAAAGGAAAAGCAAGCTTCCTGAGGGAGAGGGGAAATCCCCCCTGAGTGGGAATATACCAG GGAAAAAACAGCAGGGAACCAAAATAGCAGAGATAGAGGAGGACACCAAAGGCAAAGCTGGCACTGTGTC TTCAAGAGACAATCCACACCTCAGCACTTTTAACCTAGAGAGAACTGCCTTTCGCAAGGACAGCCAAAGA TGGTATGTGGCCTCTGATGGTGGGGTGGTAGAGAAAAGTGGAATGGAAGCACCAGCCATGAAAGTCTTTC CCAGAGGTCCAGGCTTGGGTAACAGAGAGGCTGAAGGGAAAGAGGATGGCACTGTGGAAGGAGGGGCTGA TGATGCTTCAGTACTTGGCCAAGGGGACCGCTTCCTCACAGACATGGCCTGTGTAGCCTCAGCCAAAGAC TTAGATAACCCCGAAGATACTGACTCTCCCTCTTGTGACCATGCCACTAAGCTTTCTGAGGCTGAAGACA ATGTGGCCCGCCTTTGTGACTACCATTTGGCCAAGCGAATGTCATCCCTGCAGAGTGAGGGCCATTTTTC TCTACAGAGCTCTCAAGGCTCTTCAGTGGACACAGGCTGTGGACCGGGCAGCAGTAGCAGTGCCTGTGCC ACTCCTGTGGAATCGCCCCTCTGCCCGTCCATGGGGAAGCACATGATTCCAGATGCTTCTGGGAAAGGAG GGAGATATATTTCACCAGAGGAGAGAGCCCCTGGCCATCCCAACCATGGAGCCACCTTCGAGGAACTGCA CCCACAGACAGAAGGGATGTGTCCACGCATGACAGTGCCTGCTCTGCACACAGCCATTAATGCCGACCCC CTGTTTGGCACTTTGAGAGATGGATGCCATCGACTGCCCAAGATTAAGGAAACCACAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231749 representing NM_001290428 Red=Cloning site Green=Tags(s) MMTNRDGRDYFINHMTQAIPFDDPRFDSCQIIPPAPRKVEMRRDPVLGFGFVAGSEKPVVVRSVTPGGPS EGKLIPGDQIVMINDEAVSAAPRERVIDLVRSCKESILLTVIQPYPSPKSAFISAAKKARLKSNPVKVRF SEEVIINGQVSETVKDNSLLFMPNVLKVYLENGQTKSFRFDCSTSIKDVILTLQEKLSIKGIEHFSLMLE QRIEGAGTKLLLLHEQETLTQVTQRPSSHKMRCLFRISFVPKDPIDLLRRDPVAFEYLYVQSCNDVVQER FGPELKYDIALRLAALQMYIATVTTKQTQKISLKYIEKEWGLETFLPSAVLQSMKEKNIKKALSHLVKAN QNLVPPGKKLSALQAKVHYLKFLSDLRLYGGRVFKATLVQAEKRSEVTLLVGPRYGISHVINTKTNLVAL LADFSHVNRIEMFTEEESLVRVELHVLDVKPITLLMESSDAMNLACLTAGYYRLLVDSRRSIFNMANKKN AGTQDTGSENKGKHNLLGPDWNCMPQMTTFIGEGEQEAQITYIDSKQKTVEMTDSTLCPKEHRHLYIDNS YSSDELNQPLTQPGDAPCEADYRSLAQRSLLTLSGPDTLKKAQESPRGAKVSFIFGDLALDDGMSPPTIG YERMLEENPEMLEKQRNLYISSANDMKNLDLTPDTDSIQFVANSVYANIGDVKNFEAPEGIEEPLLHDIC YAENTDDAEDEDEVSCEEDLVVGEMNQPAILDLSGSSDDIIDLTTLPPPEGDDNEDDFLLRSLNMAIAAP PPGFRDSSDEEDTQSQATSFHEDKEQGSSLQNEEIPVSLIDAVPTSAEGKCEKGLDPAVVSTLEALEALS EEQQKSENSGVAILRAYSPESSSDSGNETNSSEMTEGSELAAAQKQSESLSRMFLATHEGYHPLAEEQTE FPTSKAPSVGLPPKSSHGLAARPATDLPPKVVPSKQILHSDHMEMEPETMETKSVTDYFSKLHMGSVAYS CTSKRKSKLPEGEGKSPLSGNIPGKKQQGTKIAEIEEDTKGKAGTVSSRDNPHLSTFNLERTAFRKDSQR WYVASDGGVVEKSGMEAPAMKVFPRGPGLGNREAEGKEDGTVEGGADDASVLGQGDRFLTDMACVASAKD LDNPEDTDSPSCDHATKLSEAEDNVARLCDYHLAKRMSSLQSEGHFSLQSSQGSSVDTGCGPGSSSSACA TPVESPLCPSMGKHMIPDASGKGGRYISPEERAPGHPNHGATFEELHPQTEGMCPRMTVPALHTAINADP LFGTLRDGCHRLPKIKETTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290428
ORF Size	3840 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290428.1, NP_001277357.1
RefSeq Size	8156 bp
RefSeq ORF	3843 bp
Locus ID	333605
UniProt ID	A2AFR3
MW	140.9 kDa
Gene Summary	Positive regulator of dendritic spine morphogenesis and density. Required for the maintenance of excitatory synaptic transmission. Binds phosphatidylinositol 4,5-bisphosphate.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.