Mtus1 (NM_001286413) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI481402; ATBP135; Atip1; B430010I23Rik; B430305I03Rik; C85752; Cctsg1-440; MD44; mKIAA1288; MTSG1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231687 representing NM_001286413 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGATGATAATTCAGATAGGACAGAAGATGGATCGCGTTATGTATTTATCCGAGATAAAAACTCGA ATCCATCTGAATACTACCAAACATCGCTATCTGCCCAGTGCCCTTCAGTGAGTCACGGGGATTGGAACTC TGACAACCCAGATGCCATGGTGGTCGATTATGAAATGGATCCTGCGGTGGACAGTAGTGAAAGTGTATCT TTAAGCCATCAGTGTGTTGAAGAACTTGCTTACCCAGAGCCTTCAAGTGATTTCATGGGGAAGCATGAGT TTACAATGTACAGTGAGCTTACCTGCCAGTCTCCTGCACTTGTGAACACAGGGAAGCCCCAAGACCTGCA CAGTAACTGTGACTCCCTAGAGGCAATCCAGGATGAGAAGTTTGATCCCCTTAAGCCCTGTGAGTGCAGG TCTGATGACGATTATGCTTGTGGAGATTCCCCTGAAGTTTTGGAGCTAAAACAAACATATGGCATGAAAG TGGACACGGCTAACTACACCTTTATAGCACGCCATGATATTGAACAGGGTCAGCCTTTGCATGCTCCTGG AGGTTTGCAGACAACAGTGAGAGACAGAAATGCTTTGTCCTCCTGCGGTCGGACACCACCCCATTCCTCT AAGATGTATGTGAGAGGGGTTAATTATAATCGAGAGAACTTTGAAAACCTCCAAGCCACACCATCCAAGA CCCTAAACACAACCTTTACAGTGATCTCTGATGTGCTGATGCAAACTGACAGTCCAGACGTTGGAGTTCA GGGTCAGAATTCTTTAGGAAATGTCACCAAAGAGTACACAGATGGGACAAGGCGAGGACTCATTGGAGAA AAAGAGATACAAGCTGTGACACTGGTTTCGGATGGCATGGAGGTCCCCAACGGATCTGCATCACAGGAGT TCTATTGTGTATCTGAAGATGACCCAAACAGTGAGACACATTCACACGGCCCGTATGCTCAACAGGAAAT GGGCCAAAATTTAAGAGGAACACTGCCCAACTGTCATGTCGATGGTGAATGCCCAGTACTGGTGCCAGCT TTTGAGAAGAGTAAAACCAGAGTCCTGGGCTCTGAGTGTAAAGTCACTGTGACTGAAGACCCACACATCG ATTCCCACGACAATGACTCAGACATCCAAAGTAGTACTGAAGAGCTGACCCTGAGAAGTGTTTCAGGGCA GAGGGGTTCACCTTATGAAATGGGTTGGGGCGAGAACGGCGGAGCCATTTGCACAGATAAAGCAGGTTGC ATGTCAACACCAGTTGAGCAACCCCCCAATCTGAGCTTCAGACTTGAACCTGCTGAAGTGAAGAAGTACA ATAATGTGGAGAATGGACCAAGAGATGCTAAACGTGCACCAAACTTGAAAGGGGAACCAACAAATATGCC AAAACCTAATTTGGGAAAATCAGCTACCAAAACAAATACCACTGTAGGCAGTAAAGTCAGGAAGACAGAA ATTATAAGTTATCCTACACCAAACTTCAAAAACATTAAAGCCAAAGTTATATCCAGATCAGTGTTGCAAC CCAAAGACACTTCCATAATGAAGGACACACCCAGCCCTCAGGTAACAGGGGGCTCTTCACCATCACCAGG GCCTTCTAAACACTTGACAATGATGAACAAAGCACCAAGATCTGACTTTAAAGCCAGCAAAAAAGCAGAA ATCCCAATTAACAAGACACACAAGCAACAGTTTAATAAACTCATTACCAGCCAGGCTGCGCAAGTTACAA CTCATTCTAAAAATGCTTCACTCGGGGTTCCAAGAACAACATCTGCCACGAAATCGAATCAGGAGAATGT TGACAAGACAGGTTCTCCTCATGCAGGGAGTGAGACCGGGTCTGTAGCTGCCTTCTTCCAGAAGATCAAA GGCATTCTCCCCGTGAAAATGAAAAGCTCAGAGTGTTTGGAAGTGACTTACGTTTCCCACATCGATCAGA TTAGCCCTGAAAAGGGTGAGCAGGACGGTGAGGCTCCCATGGAAAAGCAAGAGCTGGGAAAACAAGCCAC GAATGAGATTTTTGAATCCAAGTCTCTATTGGTGGGCTCTGCACCCAAAACTTCCACCACGCCAGGAAGG AGCAGTTCCAAGCCAGACTCGCGGAGCCTGAGGAAAACACCAGGTCTAAAAGCCAAAGTGGGTCCCACTG CTGCCTGCCTGAGGAGGAAGAGTGAGAGTAGAACCCTAGGCTCCGACAGGGCCTTATCGCCTCAGAGGAT CAGGCGCGTGTCCGGCTCTGGTGGTCATGCTGCCATCAACAAATATGAAGAGAAACCTCCCAAACAAGCA TTTCAGAATGGGTCAGGACCCTTGTATCTGAAGCCTTTGGTACCCAGAGCTCACTCGCACTTGCTCAAAA CTTCTCCAAAAGGTCCTTCAAGGAAGAGTCTGTTTACAGCTTTTAATTCAGTTGAAAAGGGCAGGCAGAA GAATCCCAGGAGCCTATGCATCCAGACCCAGACAGCTCCAGATGTGCTGTCCTCCGAGAGAACGCTTGAG TTGGCCCAATACAAGACAAAATGTGAAAGCCAAAGTGGATTCATCCTGCACCTCAGACAGCTTCTTTCCC GTGGTAACACCAAGTTTGAAGCGCTGACCGTTGTGATCCAGCACCTCCTGTCTGAGCGGGAGGAAGCACT GAAGCAACACAAAACCCTCTCTCAAGAACTTGTCAGCCTCCGGGGAGAGCTAGTTGCTGCTTCAAGCGCC TGTGAGAAGCTAGAAAAGGCTAGGACTGACTTACAGACAGCGTATCAAGAATTTGTCCAGAAACTAAACC AGCAGCATCAGACAGACCGGACGGAACTGGAGAACCGGCTGAAGGACTTATACACCGCAGAGTGTGAGAA GCTTCAGAGCATTTACATTGAGGAGGCAGAAAAATATAAAACTCAACTGCAAGAGCAGTTTGACAACTTA AACGCCGCCCATGAGACCACTAAGCTTGAGATTGAAGCTAGCCACTCGGAGAAGGTGGAATTGCTGAAGA AGACCTATGAAACCTCCCTTTCAGAAATCAAGAAGAGCCATGAGATGGAGAAGAAGTCACTGGAGGATCT GCTTAATGAGAAGCAGGAATCGCTGGAGAAACAAATCAATGATCTGAAGAGTGAAAACGATGCTTTAAAC GAAAGGTTGAAATCAGAGGAGCAAAAGCAACTGTCAAGAGAGAAGGCGAATTCAAAAAACCCTCAGGTCA TGTATCTGGAGCAAGAACTAGAAAGCCTGAAGGCTGTGTTAGAGATCAAGAATGAGAAGCTGCACCAGCA GGACATGAAGCTAATGAAGATGGAAAAGCTGGTGGACAATAACACAGCATTGGTTGACAAGCTGAAGCGA TTCCAGCAGGAAAACGAGGAGTTAAAAGCTCGCATGGACAAACACATGGCAATTTCAAGGCAACTTTCCA CCGAGCAGGCCGCGCTGCAGGAGTCCCTTGAGAAGGAGTCGAAGGTCAACAAGAGACTGTCCATGGAGAA CGAGGAACTTCTGTGGAAACTGCACAACGGAGACCTGTGCAGCCCCAAGAGATCCCCCACCTCCTCGGCC ATCCCTTTCCAGTCCCCCAGGAATTCTGGTTCCTTCTCCAGCCCCAGCATCTCACCCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231687 representing NM_001286413 Red=Cloning site Green=Tags(s) MNDDNSDRTEDGSRYVFIRDKNSNPSEYYQTSLSAQCPSVSHGDWNSDNPDAMVVDYEMDPAVDSSESVS LSHQCVEELAYPEPSSDFMGKHEFTMYSELTCQSPALVNTGKPQDLHSNCDSLEAIQDEKFDPLKPCECR SDDDYACGDSPEVLELKQTYGMKVDTANYTFIARHDIEQGQPLHAPGGLQTTVRDRNALSSCGRTPPHSS KMYVRGVNYNRENFENLQATPSKTLNTTFTVISDVLMQTDSPDVGVQGQNSLGNVTKEYTDGTRRGLIGE KEIQAVTLVSDGMEVPNGSASQEFYCVSEDDPNSETHSHGPYAQQEMGQNLRGTLPNCHVDGECPVLVPA FEKSKTRVLGSECKVTVTEDPHIDSHDNDSDIQSSTEELTLRSVSGQRGSPYEMGWGENGGAICTDKAGC MSTPVEQPPNLSFRLEPAEVKKYNNVENGPRDAKRAPNLKGEPTNMPKPNLGKSATKTNTTVGSKVRKTE IISYPTPNFKNIKAKVISRSVLQPKDTSIMKDTPSPQVTGGSSPSPGPSKHLTMMNKAPRSDFKASKKAE IPINKTHKQQFNKLITSQAAQVTTHSKNASLGVPRTTSATKSNQENVDKTGSPHAGSETGSVAAFFQKIK GILPVKMKSSECLEVTYVSHIDQISPEKGEQDGEAPMEKQELGKQATNEIFESKSLLVGSAPKTSTTPGR SSSKPDSRSLRKTPGLKAKVGPTAACLRRKSESRTLGSDRALSPQRIRRVSGSGGHAAINKYEEKPPKQA FQNGSGPLYLKPLVPRAHSHLLKTSPKGPSRKSLFTAFNSVEKGRQKNPRSLCIQTQTAPDVLSSERTLE LAQYKTKCESQSGFILHLRQLLSRGNTKFEALTVVIQHLLSEREEALKQHKTLSQELVSLRGELVAASSA CEKLEKARTDLQTAYQEFVQKLNQQHQTDRTELENRLKDLYTAECEKLQSIYIEEAEKYKTQLQEQFDNL NAAHETTKLEIEASHSEKVELLKKTYETSLSEIKKSHEMEKKSLEDLLNEKQESLEKQINDLKSENDALN ERLKSEEQKQLSREKANSKNPQVMYLEQELESLKAVLEIKNEKLHQQDMKLMKMEKLVDNNTALVDKLKR FQQENEELKARMDKHMAISRQLSTEQAALQESLEKESKVNKRLSMENEELLWKLHNGDLCSPKRSPTSSA IPFQSPRNSGSFSSPSISPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286413
ORF Size	3630 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286413.1, NP_001273342.1
RefSeq Size	6432 bp
RefSeq ORF	3633 bp
Locus ID	102103
UniProt ID	Q5HZI1
MW	134.4 kDa
Gene Summary	Cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. May be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation.[UniProtKB/Swiss-Prot Function]

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