Tyk2 (NM_001205312) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JTK1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231681 representing NM_001205312 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGACCATGCCTCTGTGTGGGCGGAGAGCCATCTTGGAAGACAGCAAGGCAGATGGCACAGAAG CTCAGCCCCTGGTGCCCACAGGATGCTTGATGGTGCTACTGCATTGGCCTGGGCCTGAGGGCGGGGAGCC CTGGGTCACCTTCAGCCAGACATCTCTGACTGCAGAGGAGGTCTGCATCCACATCGCACACAAAGTCGGC ATCACTCCACCCTGCTTGAATCTCTTCGCACTCTACAATGCACAGGCTAAGGTCTGGCTGCCCCCAAACC ATATTCTGGATACATCCCAAGACATGAACCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTGGCATGG CATGAATCCCCAGGAGCCAGCTGTATACCGGTGTGGTTTCCCAGGGGCAGAGACTTCCTCAGACCGGGCA GAGCAAGGTGTACAGCTCTTGGACTCTGCCTCGTTTGAATACCTTTTTGAGCAGGGATGGTATGTTGGCA ATAGAGCAAAAAGCTCCCACACCTCTTTTTCTATCCATCGCTCTACCCCTCAGGGAAAGCATGAGTTCAT GAACGATGTGGTGTCTCTGCGGGACCTGTCTAGCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCTTA GGCATGGCCTTCCTGCACCTGTGTCACCTTGCTCTCAGCCGAGGCGTCCCCCTGGAGGAGATGGCCAGAG AGATCAGCTTCAAGAACTGCATCCCTCATTCCTTCCGACAGCACATCCGGCAGCACAATGTGCTCACACG CCTGCGGCTCCACAGAGTCTTCCGCCGCTTCCTGCGGGCCTTCCGGCCTGGCCACCTCTCCCAGCAGGTT GTGATGGTGAAGTACTTGGCTACCCTGGAGCGGCTGGCTCCGCGCTTTGGCTCAGAGCGCATACCTGTGT GTCATCTGGAGGTCCTGGCACAGCCCGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGACCGCTGG GGATCCAGGCCCAGAGCTGCCTTCCGGGCCTCCCACCCACGAGGTACTGGTGACAGGCACCGGAGGTATC CAGTGGCATCCGCTGCAGACCCAGGAATCTGAGAGAGGTAACAGCAGAGGGAATCCCCACGGCAGCCGGT CGGGGAAGAAGCCCAAAGCCCCCAAGGCTGGAGAGCACCTGACAGAGAGCCCTCAGGAACCACCTTGGAC CTACTTCTGTGACTTCCAGGACATTTCCCACGTGGTGCTAAAGGAGCGTCGCGTGCACATCCACCTCCAA GACAACAAGTGCTTGTTGCTGTGCCTCTGTTCCCAGGCTGAGGCCCTGTCCTTTGTGGCCCTGGTCGATG GCTATTTCCGCTTGACTGCTGACTCCAGCCACTATCTGTGCCATGAGGTGGCACCCCCGAGGCTGGTGAC TAGCATCCAGAACGGCATCCATGGGCCCCTGATGGATCCGTTTGTACAGGCCAAGCTGTGGCCAGAGGAC GGCCTCTACCTGATTCAGTGGAGCACCAGCCACCTGCACCGCCTGATCCTTACCGTGGCCCATCGAAACC CGGCTTTCAGTAATGGCCCTCGGGGCCTGCGCCTGCGAAAGTTCCCCATCACACAGCAGCCTGGAGCCTT TGTGCTGGATGGCTGGGGCCGCTCCTTTGCCAGCTTGGGGGACCTTCGGCTCGCCTTGCAGGGCTGCTCG TTGCGGGCCGGTGATGACTGTTTCCCCCTGCACCACTGCTGCCTGCCCCGGCCAAGAGAAATCTCCAACC TCGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCTCTCAACCTCAGTCAGCTCAGCTTCCACAGGGT TCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGAGGGCCTT CTAAGAGTGGGAGGCCCCGATGAGGGCAAAGTGGACAATGGATGTCCCCCTGAGCCTGGTGGGACTAGTG GGCAGCAGCTTCGAGTGGTGCTCAAAGTTCTCGACCCCAGTCACCATGATATCGCCTTGGCCTTCTATGA GACTGCTAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTTTGCGTGCGTGGC TCAGAGAATATCATTGTGACAGAATTCGTAGAACATGGTCCCTTGGATGTGTGGTTACGGCGACAGAGGG GCCAAGTGCCCATGACCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTCAGCTACCTGGAGGA CAAGAATCTGGTTCACGGGAATGTATGTGGCCGGAACATCCTGCTGGCTCGCCTGGGGTTGGAGGAGGGT ACCAACCCCTTCATCAAGCTAAGTGATCCTGGTGTGGGCCAGGGTGCCCTCTCCAGGGAAGAGCGGGTGG AGCGCATCCCCTGGACAGCTCCCGAGTGCCTGTCTGGAGGGACCAGTAGCTTGGGTACTGCCACGGACAT GTGGGGCTTTGGTGCCACCCTTCTTGAGATCTGCTTTGATGGGGAGGCACCCCTGCAGGGTCGTGGTCCC TCTGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCTGAACCCTCATCCCCAGAGCTGGCCACAC TCACCCGCCAGTGCCTGACCTATGAACCAGCACAGCGGCCATCCTTCCGCACCATTTTGCGGGACCTCAC AAGGCTGCAGCCACAGAATCTAGTCGGCACTTCGGCTGTGAACTCAGACTCACCAGCATCAGACCCCACT GTTTTCCACAAGCGCTATTTGAAAAAGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTCAGCCTGT ACTGCTACGACCCAACCAATGACGGCACTGGCGAGATGGTGGCCGTGAAGGCCCTGAAGGAAGGGTGCGG TCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAGATCCTGCGGACGCTGTACCACGAACATATTGTC AAGTATAAAGGCTGCTGTGAGGACCAAGGAGAGAAGTCTGTACAGCTGGTCATGGAGTACGTCCCTCTGG GCAGCCTCCGAGACTACCTGCCAAGGCACTGCGTAGGGCTGGCGCAGCTCCTGCTGTTTGCCCAGCAGAT CTGCGAGGGCATGGCCTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCCGCGCGCAACGTGCTG CTGGACAACGACAGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGCCACGAGT ACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTGTTCTGGTATGCCCCAGAATGCCTGAAGGAGTGCAA ATTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTAACTCTGTATGAGTTGTTGACATACTGTGACTCT AACCAGAGTCCCCATATGAAATTCACCGAGCTCATCGGCCATACCCAGGGCCAGATGACCGTGCTGAGGC TCACAGAGCTGCTGGAACGAGGAGAGAGGCTGCCTCGGCCTGACAGGTGTCCCTGTGAGATCTATCACCT CATGAAGAACTGCTGGGAGACAGAGGCCTCCTTCCGGCCCACCTTCCAGAATCTTGTGCCCATCCTCCAG ACAGCACAGGAGAAGTACCAAGGTCAGGTGCCTTCCGTGTTCAGCGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231681 representing NM_001205312 Red=Cloning site Green=Tags(s) MVGTMPLCGRRAILEDSKADGTEAQPLVPTGCLMVLLHWPGPEGGEPWVTFSQTSLTAEEVCIHIAHKVG ITPPCLNLFALYNAQAKVWLPPNHILDTSQDMNLYFRMRFYFRNWHGMNPQEPAVYRCGFPGAETSSDRA EQGVQLLDSASFEYLFEQGWYVGNRAKSSHTSFSIHRSTPQGKHEFMNDVVSLRDLSSEEEIHHFKNESL GMAFLHLCHLALSRGVPLEEMAREISFKNCIPHSFRQHIRQHNVLTRLRLHRVFRRFLRAFRPGHLSQQV VMVKYLATLERLAPRFGSERIPVCHLEVLAQPERDPCYIQNSGQTAGDPGPELPSGPPTHEVLVTGTGGI QWHPLQTQESERGNSRGNPHGSRSGKKPKAPKAGEHLTESPQEPPWTYFCDFQDISHVVLKERRVHIHLQ DNKCLLLCLCSQAEALSFVALVDGYFRLTADSSHYLCHEVAPPRLVTSIQNGIHGPLMDPFVQAKLWPED GLYLIQWSTSHLHRLILTVAHRNPAFSNGPRGLRLRKFPITQQPGAFVLDGWGRSFASLGDLRLALQGCS LRAGDDCFPLHHCCLPRPREISNLVIMRGSRAHTRPLNLSQLSFHRVHQDEITQLSHLGQGTRTNVYEGL LRVGGPDEGKVDNGCPPEPGGTSGQQLRVVLKVLDPSHHDIALAFYETASLMSQVSHMHLAFLHGVCVRG SENIIVTEFVEHGPLDVWLRRQRGQVPMTWKMVVAQQLASALSYLEDKNLVHGNVCGRNILLARLGLEEG TNPFIKLSDPGVGQGALSREERVERIPWTAPECLSGGTSSLGTATDMWGFGATLLEICFDGEAPLQGRGP SEKERFYTKKHQLPEPSSPELATLTRQCLTYEPAQRPSFRTILRDLTRLQPQNLVGTSAVNSDSPASDPT VFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKEGCGPQLRSGWQREIEILRTLYHEHIV KYKGCCEDQGEKSVQLVMEYVPLGSLRDYLPRHCVGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVL LDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKECKFYYASDVWSFGVTLYELLTYCDS NQSPHMKFTELIGHTQGQMTVLRLTELLERGERLPRPDRCPCEIYHLMKNCWETEASFRPTFQNLVPILQ TAQEKYQGQVPSVFSVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205312
ORF Size	3621 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205312.1, NP_001192241.1
RefSeq Size	4873 bp
RefSeq ORF	3624 bp
Locus ID	54721
MW	135.9 kDa
Gene Summary	Involved in intracellular signal transduction by amplifying type I and type II IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. Plays an essential role in promoting selective immune responses, including innate host defense mechanisms and specific antiviral activities.[UniProtKB/Swiss-Prot Function]

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