Dhx30 (NM_001252682) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2810477H02Rik; C130058C04Rik; Ddx30; HELG; Ret-CoR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231673 representing NM_001252682 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTTGGACTCATTCAGAAAAGATCGGACCCAGCACAGGCAGCGTCAATGCAAGCTTCCCCCAC CCCGTCTTCCACCCATGTGTGTCAACCCTGCCCCTGGAGGGACCATCACTCGAGCTTCTAGGGACCTATT AAAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCCTTGGCATCTCACATGCA AAAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCACCCTGCACATAAAGTGGC CCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGTCAGGCTGCAGCAGCTGC CTGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTGATGCAGCTAAATACCGA GTGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACCCACCATGCCTCCAACTT CCTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTATCCCGATCCTTAGGCCG AGAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGACAGAGTTTCTGTCTATG ACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGAAATGACAGATGATGACA GTGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAAGTGATTCAGATTGCAAC CTCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGACCAAGCTGGCTACACTC ACTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGCTGAGGCTGAGAATAAGG CAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAACAATGAGCCGCTTACCCA TGCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGCCATGTACCATCCAGGTG CCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGACAGCTCATGGATTTCCC CAGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGGCCCTTGAGTGACCCTAT CACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCCAGAGCCTGCTAGAGCTG TGGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCCTCATCGGGACACTATCC TCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGTGGGAAGACCACACGTAT CCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCAATGTGATCATCACACAA CCTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGGCCCCTCCTTGCGCCGGA ATGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCGCTGCTCTTCTGCACTGT GGGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCCATGTCATTGTGGATGAG GTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCTGCAGCGGCTCAACCCAG CTCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGATACTTTGGGGGCTGCCC TGTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGGACATCTTGGCCAAGCTG GGTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATGTGCACTTGATTTGGACC TTGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATCCTGTGCTTTCTACCTGG CTGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGCATGAAAGCAAGTACCTC ATCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCAACAGCCTCCACTTGGGG TACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAACGACATTGTACATGTCGT GGACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACTGTGTGG GTGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTCAGGTTTTGCCTACCACT TGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGA GAACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGTTCCTCTCTAAGGCTGTG GACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGATTGGGGTGCTGGACCAGC GGGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCCCGACTGGCCAAGGCCAT AGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCC TTCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCTGAGCCATGACAGTGGCA GTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGCTGGCAGGACCGTACCTC CAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCATCCACGGGCTCATCAAG CAGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTGCACACTGCCCTCTGCTC AGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCTGGCCTCTACCCCAACCT CATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACAGTGTCACTTACAGGACC AAATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCGGTTACGGAGCCGATGGC TGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCCTCCCAGGTGCACCCACT AGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTCGGGCCACCATCTCATTG AGTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCTAAGGGAGTTTCGTCGAG CCCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCTCTTAGTGTGCAGCAAGA ACACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCAGCTTTGATATGCGCAAG ACAGCTGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231673 representing NM_001252682 Red=Cloning site Green=Tags(s) MFSLDSFRKDRTQHRQRQCKLPPPRLPPMCVNPAPGGTITRASRDLLKEFPQPKNLLNSVIGRALGISHA KDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYR VLADRFGSPADSWWRPEPTMPPTSWRQLNPENIRPGGPAGLSRSLGREEEEDEEEELEEGTIDVTEFLSM TQQDSHNPLRDSRGGSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLATL TLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQV PEPILRKIEAFLSHYPVDSSWISPELRLQSDDILPLGKDSGPLSDPITGKPYMPLSEAEEVRLSQSLLEL WRRRGPIWQEAPQLPVDPHRDTILSAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQ PRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPARGGALLFCTVGILLRKLQSNPSLEGVSHVIVDE VHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKL GKHQYPHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYL ILPVHSNIPMMDQKAIFQQPPLGVRKIVLATNIAETSITVNDIVHVVDSGLHKEERYDLKTKVSCLETVW VSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAV DSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDP FSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRTSRENYLEENLLYAPSLRFIHGLIK QFSENIYEAFLVGKPSDCTLPSAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRT KSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISL SDSDLLRLEGDSRTVRLLREFRRALGRMVERSLRSELAALPLSVQQEHGQLLALLAELLRGPCGSFDMRK TADD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252682
ORF Size	3582 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252682.1, NP_001239611.1
RefSeq Size	4228 bp
RefSeq ORF	3585 bp
Locus ID	72831
UniProt ID	Q99PU8
MW	134.5 kDa
Gene Summary	RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function]

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