Nrde2 (NM_001290303) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6720454P05Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231652 representing NM_001290303 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGTTCCCGGCTTTTGCGGACGTAAGTGAGGCCTCCGGTGATGGGGCATTCAGGAAAGAGTTAG ATTGGCTGAGCAACCCAAGCTTTCGTGTTGGAAATCTAACTTCTCTGAGCCGACAAACTGAAGAGGTCAC AGCCCTTGCTTCTGAAGGGTCACCACCGCCAAGGTACTCTTTTATCAGGAGTCCTCTGAAGTCAGAGCTC TCAGGTGAGAGCAACACTAGCGAGAAGCTCGCACAGACAAGCAGGAAGAAGAAGAAGGAGAAAAAGAAGA GACGGAAGCATCAGCACCACAGGAAAACCAAGAGGAGGCACGAGCAGTTGAGCAGCAGCGGCTCTGAGTC AGACACAGAGGCTGGGAAGGACAGGGCTTCCCGGAGCATCAGGGACGACCAGAAGGAAGCCGAGAAACCC TGTCAGGGAAGCAATGCGGCTGCTGCTGTTGCTGCTGCTGCTGGACATCGGTCCATTTGGCTGGAAGATA TTCACGATCTGACCGACGTCTTCAGGACTGATAAGAAGCCAGATCCTGCCAACTGGGAGTATAAATCTCT GTATCGTGGGGATATAGCAAGGTACAAGAGGAAGGGAGACTCCTGCCTGGGTATCAATCCTAAGAAACAG TGTATATCTTGGGAAGGGGCTTCTGCAGCAAAGAAGCACTCACACAGGCACCTGGAGCGCTATTTTACCA AGAAGAATGTGGGGTTAATGAGAACAGAGGGCATTGCTGTGTGCAGTAACCCCGAGCCTGCCTCCTCCGA GCCCGTCACATTCATCCCAGTGAAGGACTCTGCTGAGGCAGCTACTCCTGTTACATCATGGCTGAATCCT CTGGGGATTTATGATCAGTCTACCACACAGTGGTTACAAGGACAGGGTCCAGCAGAGCAAGAGTCAAAGC AGCCGGACTCGCAACAGGACAGGGAGAATGCTGCTCTCAAGGCCAGAGTGGAAGAGTTTAACAGAAGGGT TCGGGAGAATCCTTGGGACACGCAACTATGGATGGCGTTTGTGGCTTTCCAGGACGAGGTCATGAGGAGT CCTGGCATCTATGCCCTTGGGGAAGGAGAACAGGAGAAGCACAGGAAGTCCCTGAAGCTCCTCCTGGAGA AGAAGCTGGCTGTGCTGGAGCGGGCCATCGAGAGCAACCCAGGCAGCGTGGAGCTGAAGCTTGCCAAGCT GCAGCTGTGCTCAGAGTTCTGGGAGCCCAGTGCGCTGGCCAAGGAGTGGCAGAAGCTCCTCTTCCTGCAC CCCAACAACACAAGCCTGTGGCAGCGCTATCTCTCCTTTTGCCAGAGCCAGTTTGGTACCTTTTCTGTGT CCAAACTCCACAGTCTGTATGGCAAGTGCTTGAGTACCTTGTCTGCTGTTAAAGATGGCAGCATGCTATC CCACCCTGTGTTGCCGGGCACCGAGGAGGCCATGTTTGGGCTCTTTCTTCAGCAGTGCCACTTTCTACGG CAGGCTGGCCACTCAGAGAAGGTCATCTCTTTGTTCCAGGCCATGGTTGACTTCACCTTCTTCAAGCCTG ACAGTGTGAAAGAGCTGCCTACTAAAGTACAGGTGGAATTCTTTGAGCCCTTCTGGGACAGCGGAGAACC ACGCGTTGGGGAGAAGGGAGCCCGAGGCTGGCGGGCGTGGATGCACCAACAGGAGCGAGGTGGCTGGGTG CTCATCACCCCAGATGAGGATGACGAGGAGCCCGAAGAGGAGGACCAGGAGATAAAAGACAAGACCCTGC CCCGGTGGCAGATCTGGCTTGCTGTTGAGCGTTCTCGAGACCAGAGGCACTGGCGTCCCTGGCGCCCTGA TAAGACCAAGAAGCAAACGGAGGAAGACTGTGAGGACCCTGAGAGACAGGTGCTGTTTGATGACATTGGG CAATCTTTGATCAGACTTTCCAGCCCAGACCTTCAGTTCCAGCTCATCCAGGCCTTTCTGCAGTTCCTGG GCGTGCCTTCCGGCTTCCTGCCCCCAGCCTCCTGCCTTTACCTGGCCATGGACGAGAGCAGCATCTTTGA GAGTGAACTTTATGATGAAAAGCCCCTGACTTACTTCAACCCTTCCTTTTCGGGCATTAGCTGTGTCGGC TCCATGGAACAGTTGGGTCGCCCTCGCTGGACCAAAGGTCACAACCGAGAGGGCGAGGAGTTTGTCCGCA ATGTCTTCCACCTGGTGCTGCCTTTGCTCGCAGGCAAGCAGAAGTCCCAGCTCAGCCTCTCCTGGTTACG GTACGAGATTGCAAAGGTCATTTGGTGTCTACACACTAAAAAGAAGCGATTGAAGTCTCAGGGAAAGAGC TGCAAAAAACTAGCCAAGAATCTCCTCAAGGAGCCAGAAAACCGCAACAACTTCTGCCTCTGGAAGCAGT ATGCACACCTGGAGTGGTTGCTCGGTAACACAGAGGATGCCAGAAAAGTGTTCGACACAGCACTCAGCAT GGCAGGCAGCAGCGAGCTGAAGGACCGTGAACTCTGCGAGCTCAGTCTGCTCTATGCCGAGCTGGAGATG GAACTGTCGCCGGACTCGAGAGGAGCCACCACAGGCCGAGCGGTCCACATATTAACACGGCTGACGGAGA GCAGTCCCTACGGGCCCTACACGGGGCAGGTCTCGTCCACCCAGGTGCTGAAAGCTCGGAAGGCTTACGA GCTCGCGCTGCAGGACTGCCTGGGCCAGAGCTGTGCCTCCAGCCCAGCTCCTGCAGAGGCCTTGGACTGC CTGGGTAGCTTGGTCCGATGCTTTATGCTCTTCCAGTATTTGACTGTGGGCATTGATGCTGCTGTGCAGA TATACGGAAGGGTGTTTGCCAAGCTGAAGGGCTCTGCTCGCTTAGAAGACCCAGGGCCAGAGGACAGCAC CAGCTCCCAGAGTTTGACCAATGTGCTCGAGGCTGTCAGCATGATGCACACAAGCCTGCTGCGATTCCAC ATGAACGTTTGTGTTTACCCTCTGGCTCCACTCCGAGAGACCCTCTCCGATGCTTTAAAGCTGTATCCGG GCAACCAGGTTCTTTGGAGGGCTTATGTACAGATTCAGAATAAGTCCCACAGTGCTAACAAGACCAGAAG GTTCTTTGACACAGTTACTAGGTCTGCCAAACATTTGGAGCCTTGGCTATTTGCAATCGAAGCTGAGAAG CTGAGGAAAAAGCTAGTGGAATCTGTTCAGAGGGTAGGAGGCAGAGAGGTCCATGCAACAATCCCCGAGA CTGGCCTTACGCATCGCATCAGAGCTTTGTTTGAAAATGCGATTCGGAGCGACAAGGGCAACCAGTGTCC CCTACTGTGGAGGATGTATTTGAATTTTTTGGTTTCCTTAGGAAACAAAGAAAGAAGCAAAGGCGTGTTC TACAAGGCCCTCCAGAGCTGTCCTTGGGCAAAGGTGCTATACATGGACGCCATGGAGTATTTCCCAGACG AGCTGCAGGAGATCCTGGACGTGATGACCGAGAAGGAGCTCCGTGTGCGCCTGCCCCTTGAGGAGCTGGA GCTGCTGCTGGAGGAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231652 representing NM_001290303 Red=Cloning site Green=Tags(s) MALFPAFADVSEASGDGAFRKELDWLSNPSFRVGNLTSLSRQTEEVTALASEGSPPPRYSFIRSPLKSEL SGESNTSEKLAQTSRKKKKEKKKRRKHQHHRKTKRRHEQLSSSGSESDTEAGKDRASRSIRDDQKEAEKP CQGSNAAAAVAAAAGHRSIWLEDIHDLTDVFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQ CISWEGASAAKKHSHRHLERYFTKKNVGLMRTEGIAVCSNPEPASSEPVTFIPVKDSAEAATPVTSWLNP LGIYDQSTTQWLQGQGPAEQESKQPDSQQDRENAALKARVEEFNRRVRENPWDTQLWMAFVAFQDEVMRS PGIYALGEGEQEKHRKSLKLLLEKKLAVLERAIESNPGSVELKLAKLQLCSEFWEPSALAKEWQKLLFLH PNNTSLWQRYLSFCQSQFGTFSVSKLHSLYGKCLSTLSAVKDGSMLSHPVLPGTEEAMFGLFLQQCHFLR QAGHSEKVISLFQAMVDFTFFKPDSVKELPTKVQVEFFEPFWDSGEPRVGEKGARGWRAWMHQQERGGWV LITPDEDDEEPEEEDQEIKDKTLPRWQIWLAVERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIG QSLIRLSSPDLQFQLIQAFLQFLGVPSGFLPPASCLYLAMDESSIFESELYDEKPLTYFNPSFSGISCVG SMEQLGRPRWTKGHNREGEEFVRNVFHLVLPLLAGKQKSQLSLSWLRYEIAKVIWCLHTKKKRLKSQGKS CKKLAKNLLKEPENRNNFCLWKQYAHLEWLLGNTEDARKVFDTALSMAGSSELKDRELCELSLLYAELEM ELSPDSRGATTGRAVHILTRLTESSPYGPYTGQVSSTQVLKARKAYELALQDCLGQSCASSPAPAEALDC LGSLVRCFMLFQYLTVGIDAAVQIYGRVFAKLKGSARLEDPGPEDSTSSQSLTNVLEAVSMMHTSLLRFH MNVCVYPLAPLRETLSDALKLYPGNQVLWRAYVQIQNKSHSANKTRRFFDTVTRSAKHLEPWLFAIEAEK LRKKLVESVQRVGGREVHATIPETGLTHRIRALFENAIRSDKGNQCPLLWRMYLNFLVSLGNKERSKGVF YKALQSCPWAKVLYMDAMEYFPDELQEILDVMTEKELRVRLPLEELELLLED SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290303
ORF Size	3516 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290303.1, NP_001277232.1
RefSeq Size	3725 bp
RefSeq ORF	3519 bp
Locus ID	217827
UniProt ID	Q80XC6
MW	133.9 kDa
Gene Summary	Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation. Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation. Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome. By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm.[UniProtKB/Swiss-Prot Function]

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