Itga10 (NM_001302471) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A630048L14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231642 representing NM_001302471 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTCTCTCCATCCCTCACCTGCTCCTGCCCCTGGCGTTGCTGACAGGTCTCTGCTCCTCCTTTA ATCTGGATGAACACCACCCACGACTCTTCACAGGGCCACCAGAGGCCGAATTTGGATACAGTGTCTTACA GCATGTTGGGGGTGGACAGCGATGGATGCTGGTGGGTGCCCCCTGGGATGGGCCATCAGGTGACCGGAGA GGGGATGTTTATCGTTGCTCTATAGGGGGATTCCACAGTGCTCCATGTACCAAAGGCCACCTGGGTGACT ATCAACTTGGAAATTCCTCTCAGCCTGCTGTGAATATGCACCTAGGGATGTCTCTACTAGAGACAGATGC TGATGGGGGATTCATGGCCTGTGCCCCTCTTTGGTCTCGTGCCTGCGGCAGCTCTGTCTTCAGTTCTGGA ATATGTGCCCGTGTGGATGCTTCATTCCGGCCCCAGGGAAGCCTGGCACCCACCGCCCAACGCTGTCCCA CATACATGGATGTCGTCATTGTTTTGGATGGCTCCAACAGTATCTATCCCTGGTCAGAAGTTCAGACTTT CCTTCGGAGGCTGGTAGGAAGACTGTTCATCGATCCGGAGCAGATACAGGTAGGACTGGTACAGTACGGG GAGAACCCTGTGCATGAGTGGTCCCTGGGAGACTTCCGAACAAAGGAAGAAGTTGTGAGAGCAGCAAGGA ACCTAAGTCGGAGGGAAGGGCGAGAAACGAGAACCGCCCAAGCGATCATGGTGGCATGCACAGAAGGGTT CAGTCAGTCCCGGGGGGGACGACCAGAGGCCGCTAGGCTGCTGGTAGTTGTCACTGATGGAGAGTCCCAT GATGGAGAGGAACTTCCAGCAGCGCTAAAGGCCTGTGAGGCTGGCAGAGTGACACGTTATGGGATTGCGG TCCTTGGTCACTATCTCCGGCGACAGAGAGACCCCAGCTCTTTTCTTCGGGAAATCAGAGCTATTGCTAG TGATCCAGATGAGCGATTCTTCTTCAATGTCACCGATGAAGCTGCGCTGACAGACATTGTGGATGCACTG GGGGACCGAATTTTTGGTCTTGAAGGGTCCCGTGGAGAAAATGAAAGCTCCTTTGGGCTAGAAATGTCTG AGATTGGCTTCTCCATCCACCGACTACAGGATGGGATTCTCTTTGGGATGGTGGGGGCCTATGACTGGGG GGGCTCCGTGCTATGGCTTGAAGAAGGTCGCCGCCTTTTCCCACCACAAGCTGCCCTGGAAGATGAGTTC CCCCCTGCGTTGCAGAACCATGCGGCCTACCTGGGTTACTCTGTTTCCTCCATGCTTCTTCCCGGTGGAC GCCGCCTCTTTCTCTCAGGGGCACCGAGGTTTAGACATCGAGGAAAAGTTATCGCCTTCCAGCTAAAGAA AGATGGGGTTGTGAGGGTCGCCCAGAGCCTCCAGGGGGATCAGATTGGCTCATACTTTGGCAGCGAGCTC TGCCCGTTGGATACAGATAAGGACGGAATAACTAATATCTTACTTGTGGCGGCTCCCATGTTCCTGGGTC CCCAGAACAAGGAGACCGGACGCGTTTATGTGTACATGGTGGGCCAGCAAAATTTGCTAATGCTCCAAGG AACCCTTCAGCCAGACCGTTCCCAGGATTCTCGGTTTGGCTTTGCCATGGCTGCTCTTCCTGATCTGAAC CATGATGGTTTCACTGATGTAGCGGTGGGGGCACCCCTGGAGGATGGACACCGGGGAGCGTTGTACCTGT ATCATGGAACCCAGACTGGAATCAGGCCGCATCCTACCCAGAGGATTGCTGCTGTCTCCATGCCACAGGC CCTCCGATACTTCGGCCGAAGTGTGGATGGCCGCTTAGATCTGGATGGAGATGATCTTGTAGATGTTGCC GTGGGTGCCCACGGGGCAGCCGTTCTGTTCAGCTCCCAGCCCATCATCCACCTGATTCCAACCCTGGATG TGATGCCTCCCCACATCAGTGTGGTTCAGAAGGACTGTAAAAGACGAGGCCAGGAAGCAGCCTGTCTGAC CGCAGCCCTTTGCTTCCAAGTAGTGTCTCAAACTCCTGGGCGTTGGGATAGAAGATTCTACATAAGATTC TCAGCATCACTGGATGAGTGGACCGCTGGGGCACGTGCAGTATTCGATGGCTCTGGTCAGCGCCTGTCCC CTCGGCAGCTCCAGCTTAGTGTTGGCAATGTCACTTGTGAACAGCTGCACTTCCATGCACTGGATACATC GGATTACCTCCGGCCAGTGGCCTTGACTGTGACTTTTGCTTTGGACAACACCACGAAGCCAGGGCCTGTG CTGGCGGAAGGATCCTCTACGACTATACGGAAGCTGATCCCCTTCTCAAAGGACTGTGGCCCTGACAATG AATGTGTCACAGACCTGGTGCTTCAAGCTGACATGGACATCAGAGGCTCCAGGAAGTCCCCATTTGTGGT TCAAGGTGGACGACAGAAAGTGCTGGTGTCTGCGACCCTGGAGAACAAGAAGGAGAATGCCTACAACACT AGCCTGAGTCTCAGCTTTTCTAGAAACCTCCACCTGGCCAGTCTTACTCCTCAGGCCAAATCAGTGAAGG TGGAGTGCGCAGTCCCTTCCCCCCATACCCGGCTCTGCACCGTGGGGCATCCGGTCTTCCAGACTGGGGC CAAGGTGAGCTTCCTGTTAGAGTTTGAATTTAGCTGCACCTTCCTCCTGAGCCAGGTCTTTGTGAGGCTG ACTGCCAGTAGCAGTAGCCTAGAGATGAATGAGACCCTTCAAGATAACACAGCTCAGACCTCTGCCTACA TCCGGTACGAACCTCACCTCGTGTTCTCCAGTGAGTCCACTCTGCATCGGTATGAGGTTCACCCTTATAG GACTCTCCCAATGGGTCCTGGCCCTGAATTCAAGACCACTCTTAGGGTTCAGAATCTTGGTTGCCATGTG GTCAGTGGTCTCGTCATCTCCGCCCTCCTTCCAGCTGTAGCCCATGGGGGTAACTACTTCCTGTCACTAT CTCAAGTCATCTCTGGCAATGCAAGCTGCACGGTGCAGAACCTGACTGAGCCCCCGGGCTTCCCTGTGCA CCCAGAGGAGCTTCAGCATGCAAGCAGACTGAATGGGAGTAACAGTCGATGTCAGGTGGTAAGGTGCCAC CTTGGACTGCTGGCAAAGGGGACTGAGATCTCTGTCAGGCTGCTGAGGCTGGTTCACAATGAATTCTTTC GGAGGGCCAAGTTCAAGTCTGTGACAGTGGTCAGCACCTTCAAGTTAGGAACTGAGGAGGGCAGTGTCCT ACTACTGAATGAAGCCTCCCGCTCGAGTGAGAGTCACTTGGAGGTGATTCAGACCCACCCGACCCTCATC TCCCTGTGGATCCTCGTTGGCAGTGTCCTGGGGGGGCTGCTCCTGCTTGCTCTCCTTGTCTTCTGCCTGT GGAAGCTTGGCTTCTTTACCCGTAAGAAAATCCCCAAAGAAGAAGAAAGTGAGGAGAAGTTGGAGCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231642 representing NM_001302471 Red=Cloning site Green=Tags(s) MESLSIPHLLLPLALLTGLCSSFNLDEHHPRLFTGPPEAEFGYSVLQHVGGGQRWMLVGAPWDGPSGDRR GDVYRCSIGGFHSAPCTKGHLGDYQLGNSSQPAVNMHLGMSLLETDADGGFMACAPLWSRACGSSVFSSG ICARVDASFRPQGSLAPTAQRCPTYMDVVIVLDGSNSIYPWSEVQTFLRRLVGRLFIDPEQIQVGLVQYG ENPVHEWSLGDFRTKEEVVRAARNLSRREGRETRTAQAIMVACTEGFSQSRGGRPEAARLLVVVTDGESH DGEELPAALKACEAGRVTRYGIAVLGHYLRRQRDPSSFLREIRAIASDPDERFFFNVTDEAALTDIVDAL GDRIFGLEGSRGENESSFGLEMSEIGFSIHRLQDGILFGMVGAYDWGGSVLWLEEGRRLFPPQAALEDEF PPALQNHAAYLGYSVSSMLLPGGRRLFLSGAPRFRHRGKVIAFQLKKDGVVRVAQSLQGDQIGSYFGSEL CPLDTDKDGITNILLVAAPMFLGPQNKETGRVYVYMVGQQNLLMLQGTLQPDRSQDSRFGFAMAALPDLN HDGFTDVAVGAPLEDGHRGALYLYHGTQTGIRPHPTQRIAAVSMPQALRYFGRSVDGRLDLDGDDLVDVA VGAHGAAVLFSSQPIIHLIPTLDVMPPHISVVQKDCKRRGQEAACLTAALCFQVVSQTPGRWDRRFYIRF SASLDEWTAGARAVFDGSGQRLSPRQLQLSVGNVTCEQLHFHALDTSDYLRPVALTVTFALDNTTKPGPV LAEGSSTTIRKLIPFSKDCGPDNECVTDLVLQADMDIRGSRKSPFVVQGGRQKVLVSATLENKKENAYNT SLSLSFSRNLHLASLTPQAKSVKVECAVPSPHTRLCTVGHPVFQTGAKVSFLLEFEFSCTFLLSQVFVRL TASSSSLEMNETLQDNTAQTSAYIRYEPHLVFSSESTLHRYEVHPYRTLPMGPGPEFKTTLRVQNLGCHV VSGLVISALLPAVAHGGNYFLSLSQVISGNASCTVQNLTEPPGFPVHPEELQHASRLNGSNSRCQVVRCH LGLLAKGTEISVRLLRLVHNEFFRRAKFKSVTVVSTFKLGTEEGSVLLLNEASRSSESHLEVIQTHPTLI SLWILVGSVLGGLLLLALLVFCLWKLGFFTRKKIPKEEESEEKLEQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302471
ORF Size	3498 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302471.1, NP_001289400.1
RefSeq Size	5041 bp
RefSeq ORF	3501 bp
Locus ID	213119
MW	128.2 kDa

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