Slc4a10 (NM_001242382) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA4136; NCBE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231613 representing NM_001242382 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATGAAGAAGCCGTTG TGGATAGAGGTGGAACACGCTCTATTCTCAAAACACATTTTGAGAAAGAAGATTTAGAAGGTCATCGGAC ATTATTTATTGGAGTTCATGTGCCCCTGGGTGGAAGAAAAAGCCATCGTCGTCACAGGCATCGTGGTCAT AAGCACAGAAAGAGGGACAGAGAGAGAGATTCGGGACTGGAGGATGGAAGAGAGTCCCCTTCTTTTGACA CCCCATCGCAGAGGGTGCAGTTTATTCTTGGAACTGAGGACGATGATGAGGAGCACCTCCCTCATGACCT TTTCACAGAGCTGGATGAGATTTGCTGGCGTGAAGGGGAAGATGCTGAGTGGCGAGAGACAGCCAGGTGG TTGAAATTTGAAGAGGATGTGGAAGATGGAGGAGAAAGATGGAGTAAGCCCTATGTGGCCACGCTTTCAT TACACAGCTTGTTTGAGTTGAGAAGCTGCATCCTGAATGGAACTGTGCTACTGGACATGCATGCCAACAC GATAGAAGAAATTGCAGATATGGTCCTTGACCAGCAGGTCAGCTCAGGCCAGCTGAATGAAGATGTTCGC CACAGGGTCCACGAAGCATTGATGAAGCAGCATCATCACCAGAATCAGAAAAAACTGGCTAACAGGATTC CTATTGTCCGATCTTTTGCTGATATTGGCAAGAAACAATCAGAACCAAATTCCATGGATAAAAATGGTCA GGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGCTGAGAATAAAAATGATGTCAGCAGGGAAAACAGCACT GTAGACTTCAGCAAGGGACTGGGAGGCCAACAAAAGGGGCATACTAGTCCATGTGGGATGAAACAAAGGC TTGACAAAGGACCTCCACACCAGCAGGAGAGAGAGGTTGATCTGCATTTTATGAAAAAGATTCCTCCGGG TGCTGAAGCTTCAAACATCTTGGTAGGAGAACTGGAGTTCCTAGACAGAACTGTGGTTGCCTTTGTCAGG TTGTCTCCAGCTGTCTTGCTCCAAGGACTTGCTGAAGTTCCAATCCCAAGCAGATTTCTGTTCATCCTTC TGGGACCCCTGGGAAAGGGTCAACAGTACCACGAGATTGGCAGATCGATTGCGACCTTAATGACTGATGA GGTGTTTCATGATGTTGCTTACAAAGCTAAAGACCGCAATGACTTGGTATCAGGAATTGATGAGTTTCTG GATCAGGTTACCGTTCTTCCTCCTGGAGAATGGGATCCAAGCATACGAATAGAACCTCCCAAAAATGTCC CTTCCCAGGAGAAGAGGAAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGCGAAGCTGAGCCACA TGGAGGACACAGCGGACCTGAACTCCAGCGAACTGGGAGGATTTTTGGGGGACTTATATTAGATATCAAA AGAAAGGCTCCATTCTTCTGGAGTGACTTCAGGGATGCTTTCAGCCTGCAGTGCTTAGCATCGTTCCTGT TTCTCTACTGTGCATGCATGTCTCCTGTCATCACATTTGGAGGACTGTTGGGAGAAGCAACTGAAGGTCG TATAAGTGCAATCGAATCACTCTTTGGAGCATCTATGACCGGGATAGCCTATTCTCTTTTTGGTGGACAG CCCCTGACCATATTAGGCAGCACAGGACCTGTTTTGGTGTTTGAAAAGATCTTGTTTAAGTTTTGCAAGG AATACGGCCTGTCGTACTTGTCCTTACGGGCCAGCATTGGGCTCTGGACTGCAACACTGTGCATCATCCT TGTGGCCACGGACGCGAGCTCACTCGTCTGCTACATCACCCGGTTTACCGAAGAGGCTTTTGCTTCTCTC ATTTGCATCATTTTTATCTATGAAGCCCTGGAGAAGTTGTTTGAGCTCAGTGAAACCTATCCAATCAATA TGCACAATGATTTGGAACTGCTGACACAATACTCATGTAACTGTATGGAGCCACATAGTCCCAGCAATGA CACACTGAAGGAATGGCGGGAGTCCAACCTTTCTGCCTCTGACATAATCTGGGGGAACCTAACTGTGTCA GAGTGCAGATCACTGCACGGGGAGTATGTCGGGCGAGCCTGTGGCCATGGCCACCCCTACGTGCCAGATG TTCTCTTCTGGTCGGTGATCCTGTTCTTCTCCACAGTTACCATGTCAGCCACCCTGAAGCAGTTCAAGAC CAGCCGCTATTTCCCAACCAAGGTTCGATCCATAGTGAGTGATTTTGCGGTTTTTCTTACAATTCTGTGT ATGGTTTTAATTGACTATGCCATTGGGATCCCATCACCAAAACTACAAGTACCAAGCGTTTTCAAGCCGA CCAGAGACGACCGTGGCTGGTTTGTTACACCTTTGGGTCCAAACCCATGGTGGACAATCATAGCTGCCAT CATCCCAGCTTTACTCTGTACTATTCTGATTTTCATGGACCAGCAGATTACAGCTGTCATCATCAACAGA AAAGAGCACAAGCTAAAGAAAGGTTGTGGCTATCACCTGGATCTGTTAATGGTGGCAGTCATGCTCGGGG TCTGCTCCATTATGGGCCTGCCATGGTTTGTGGCTGCCACAGTTCTCTCCATCACTCATGTCAACAGCCT CAAGCTCGAATCAGAGTGCTCTGCTCCAGGAGAACAACCCAAGTTTCTCGGCATTCGGGAGCAGAGGGTG ACCGGGCTCATGATTTTTATTCTTATGGGTTCATCCGTTTTCATGACCAGCATTCTGAAGTTTATCCCCA TGCCAGTGTTATACGGAGTGTTTCTTTATATGGGTGCTTCGTCTCTCAAAGGAATTCAGTTATTTGATAG AATAAAGCTCTTCTGGATGCCAGCCAAACATCAACCAGATTTCATCTATCTAAGGCACGTGCCCCTCCGG AAAGTCCATCTCTTCACAGTCATTCAGATGAGTTGTCTCGGCCTTCTGTGGATAATCAAAGTTTCGAGAG CTGCTATTGTCTTTCCTATGATGGTGTTGGCACTAGTGTTTGTGAGAAAGTTGATGGACTTCTTGTTTAC CAAACGGGAACTCAGCTGGCTTGATGATTTAATGCCTGAGAGTAAAAAGAAGAAACTTGAAGATGCTGAG AAAGAAGAAGAACAAAGTATGCTAGCCATGGAGGACGAGGGCACAGTACAACTCCCACTGGAGGGACACT ACAGAGACGACCCGTCTGTGATCAATATTTCTGATGAAATGTCAAAGACTGCCATGTGGGGGAACCTTCT AGTTACTGCTGACAACTCAAAAGAAAAGGAGTCACGCTTTCCTTCTAAAAGCAATGAAAGCCGAAAGGAG AAGAAAGCTGACTCAGGGAAAGGCGTTGACAGGGAGACTTGTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231613 representing NM_001242382 Red=Cloning site Green=Tags(s) MEIKDQGAQMEPLLPTRNDEEAVVDRGGTRSILKTHFEKEDLEGHRTLFIGVHVPLGGRKSHRRHRHRGH KHRKRDRERDSGLEDGRESPSFDTPSQRVQFILGTEDDDEEHLPHDLFTELDEICWREGEDAEWRETARW LKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGTVLLDMHANTIEEIADMVLDQQVSSGQLNEDVR HRVHEALMKQHHHQNQKKLANRIPIVRSFADIGKKQSEPNSMDKNGQVVSPQSAPACAENKNDVSRENST VDFSKGLGGQQKGHTSPCGMKQRLDKGPPHQQEREVDLHFMKKIPPGAEASNILVGELEFLDRTVVAFVR LSPAVLLQGLAEVPIPSRFLFILLGPLGKGQQYHEIGRSIATLMTDEVFHDVAYKAKDRNDLVSGIDEFL DQVTVLPPGEWDPSIRIEPPKNVPSQEKRKIPAVPNGTAAHGEAEPHGGHSGPELQRTGRIFGGLILDIK RKAPFFWSDFRDAFSLQCLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFGGQ PLTILGSTGPVLVFEKILFKFCKEYGLSYLSLRASIGLWTATLCIILVATDASSLVCYITRFTEEAFASL ICIIFIYEALEKLFELSETYPINMHNDLELLTQYSCNCMEPHSPSNDTLKEWRESNLSASDIIWGNLTVS ECRSLHGEYVGRACGHGHPYVPDVLFWSVILFFSTVTMSATLKQFKTSRYFPTKVRSIVSDFAVFLTILC MVLIDYAIGIPSPKLQVPSVFKPTRDDRGWFVTPLGPNPWWTIIAAIIPALLCTILIFMDQQITAVIINR KEHKLKKGCGYHLDLLMVAVMLGVCSIMGLPWFVAATVLSITHVNSLKLESECSAPGEQPKFLGIREQRV TGLMIFILMGSSVFMTSILKFIPMPVLYGVFLYMGASSLKGIQLFDRIKLFWMPAKHQPDFIYLRHVPLR KVHLFTVIQMSCLGLLWIIKVSRAAIVFPMMVLALVFVRKLMDFLFTKRELSWLDDLMPESKKKKLEDAE KEEEQSMLAMEDEGTVQLPLEGHYRDDPSVINISDEMSKTAMWGNLLVTADNSKEKESRFPSKSNESRKE KKADSGKGVDRETCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242382
ORF Size	3405 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242382.1, NP_001229311.1
RefSeq Size	5511 bp
RefSeq ORF	3408 bp
Locus ID	94229
MW	128.3 kDa
Gene Summary	Sodium/bicarbonate cotransporter which plays an important role in regulating intracellular pH (PubMed:10993873, PubMed:20566632). Has been shown to act as a sodium/bicarbonate cotransporter in exchange for intracellular chloride (PubMed:10993873, PubMed:20566632). Has also been shown to act as a sodium/biocarbonate cotransporter which is not responsible for net efflux of chloride, with the observed chloride efflux being due to chloride self-exchange (By similarity). Controls neuronal pH and may contribute to the secretion of cerebrospinal fluid (PubMed:18165320). Reduces the excitability of CA1 pyramidal neurons and modulates short-term synaptic plasticity (PubMed:26136660). Required in retinal cells to maintain normal pH which is necessary for normal vision (PubMed:23056253). In the kidney, likely to mediate bicarbonate reclamation in the apical membrane of the proximal tubules (By similarity).[UniProtKB/Swiss-Prot Function]

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