Asap1 (NM_001276462) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231602 representing NM_001276462 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT CCGAAAGAAGTAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGGT ACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAG TGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCAC GCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACA AGAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATAT AGCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGC ACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATG ACATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTAT AGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAA TTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAA ATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGC AAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTT GAGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGC CACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGAC ATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAAT ACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCA CCGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTG TGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAAT CTTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAAC GCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGG GTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACG AGCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTC CAACTGGCCCACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAA GAGGCCTCCTCCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCACGGG CCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACA AGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCC TAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAAC ATCCCACCTGAGACTTTTCAGAAATCATCACAGTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGC TGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCT ACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAG ATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAG CCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTC CAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCC GTACCACTGCCGAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCC AGGCAGATAATGACGACGAACTCACATTTATTGAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCA GGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCAC ATCCTGTCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231602 representing NM_001276462 Red=Cloning site Green=Tags(s) MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQ NEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKG DLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKT KKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLD PKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISH ATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQS TGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDK LGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELL EAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGN TVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWN LRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFAST STDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHG PPNKGAIPWGNDVGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTN IPPETFQKSSQLTELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQ MKDLPPKPQLGDLLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETP VPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVH ILSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276462
ORF Size	3372 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276462.1, NP_001263391.1
RefSeq Size	6181 bp
RefSeq ORF	3375 bp
Locus ID	13196
UniProt ID	Q9QWY8
MW	125.2 kDa
Gene Summary	May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]

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