St18 (NM_001244692) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV348974; mKIAA0535; Myt3; NZF-3; Nzf3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231520 representing NM_001244692 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCGAGGTTGAAGATAAAACGTTGCATACTCTTTCAAAAGGAACTGAAGTTCCAATGGATTCTC TGATCCCAGAGCTCAGGGTTCCATATGATTGCTCTATGGCGAAGAAGAGGAGAGCTGAAGAGCAGGCATC GGGAGTTCCAATTAACAAGAGGAAATCTCTGCTAATGAAACCCCGGCACTACAGCCCAGACATGGGCTGC AAGGAAAGCCCAGACAACAGGAACGAGGATGATGGCCTCCTAGAAACAAACGACCATGCCACTGCAGATG AAATCATGGTAAAATCCATGGATGAAACTCTTCATTTACCTGCACAAGACAGCTCACTCCAGAAGAAAGA CCAATACACATGTTATCCAGAGCTCATGGTCAAATCTCTGGTGCACTTAGGCAAATTTGAGGAAAGTGAG TCTGTGCAGACCACATGTGAGAACTTAAATGGCAGCAGTATCCAGTCTTTAAAAGCAGAGAGTGATGAAG CACATGAAGGTTCTATGGTTCATTCCGACAATGGAAGAGACAAAGTCCACCATTCCCAGCCACCTTTCTG CTCTTCTGGTGACAGCGAAAGTGACTCTGACAGTGCAGAGAATGGGTGGGGCAATGGCTCCAACTCATCA GAAGACACTGACACTCACAAAGGCCCCAAACACAAGCTGACATACAATAGAAAGGACCTGTTGGAAGTTC CTGAGATAAAAGCTGAAGATGACAAGTTTATCCCTTGTGAGAACAGGTGTGATTCTGACACAGATGGGAG AGACCCACAGAACTCTCATATGGAACCCTTGGTTGTGAAAGCCCAGCCTTCCTTCCCAGAGGTTGAAGAG GGTGAGAGCCTGGCCACAGTAACAGAAGAGCCTGCTGAGGTGGAGAAAGCAAAGGGGAACCTGAGTTTGC TGGAACAGGCTATTGCTCTACAGGCTGAACGAGGTTCTGTCTTCCATCACACTTATAAGGAGCTGGACCG ATTTTTCCTGGACCACCTGGCAAGGGAACGGAGGCAACCTAGAGTTACTGATGCCAATGGAAGACAAATC TTTACTAATAAACATTCACCAAGACCTGAAAGGAGGGAGGCCAAGTGCCCCATTCCTGGCTGTGATGGCA CGGGGCACGTAACAGGGCTCTACCCTCACCACCGCAGCCTTTCTGGGTGCCCCCACAAAGTGCGAGTTCC TCTGGAAATTCTCGCCATGCATGAGAACGTGCTCAAGTGTCCCACGCCAGGATGCACAGGAAGGGGACAT GTGAACAGCAATCGCAACACTCACAGAAGTCTTTCTGGTTGTCCGATTGCTGCAGCTGAAAAGCTGGCAA TGACCCAGGACAAAAGTCAACTTGATTCTTCTCAGACGGGGCAGTGTCCTGAGCAGGCACACAGGGTGAA TTTGGTGAAGCAAATTGAATTTAATTTCCGCTCACATGCCATCACTTCTCCAAGAGCCTCTGCATCAAAA GAACAAGAGAAGTTTGGGAAAGTACCGTTTGATTATGCCAGTTTTGATGCACAAGTATTTGGGAAACGTC CTCTCCTACAAACAGGGCAAGGACAAAAAGCACCACCATTTCCTGAATCAAAGCATTTTTCAAATCCAGT GAAATTTCCTAATGGACTGCCTAGTGCTGGCGCCCACACACAGAGCACAGTCCGTGCCAGCTCTTATGGC CATGGTCAGTACAGTGAAGACACCCACATAGCAGCAGCTGCTGCCATCCTGAACCTTTCCACCCGCTGCA GGGAAGCCACAGACATCCTCTCCAACAAACCACAAAGCCTGCGTGCCAAGGGAGCTGAGATAGAAGTAGA TGAAAACGGCACATTGGACTTAAGCATGAAAAAAAATCGAATCCACGACAAGTCTATACCCCCAACTTCC TCACCTACTACAATTACAACCCCATCCTCATCCCCATTCAACGCAAGCAGCCTTCTGGTCAATGCTGCCT TCTATCAGGCCCTCTCTGATCAAGAAGGCTGGAATGTGCCCATCAACTATAGCAAATCCCATGGGAAGAC AGAGGAGGAGAAAGAGAAAGATCCTGTGAACTTCCTAGAAAATTTAGAGGAAAAAAAGTTTGCTGGAGAG GCCTCTATCCCAAGCCCAAAGCCCAAGCTGCATACAAGAGATCTCAAGAAAGAATTAATCACCTGTCCAA CACCAGGATGTGATGGAAGCGGCCATGTCACAGGCAACTATGCATCTCACCGCAGTGTCTCTGGATGTCC TCTAGCAGATAAGACTCTGAAGTCTCTCATGGCTGCCAACTCTCAAGAGCTTAAGTGTCCAACCCCAGGT TGTGATGGTTCTGGTCATGTGACTGGAAACTATGCCTCCCACCGAAGCTTGTCTGGTTGCCCTCGTGCAA GGAAAGGTGGCATCAAAATGACCCCAACAAAAGAAGAAAAAGAAGACTCTGAACTTAGATGCCCTGTAAT AGGGTGTGACGGCCAAGGCCACATATCAGGTAAATACACATCACACCGCACAGCTTCTGGCTGTCCTCTG GCTGCCAAAAGACAGAAAGAGAATCCCCTCAATGGGGCACCTCTCTCCTGGAAACTGAACAAGCAAGAGC TTCCTCACTGTCCTCTGCCAGGATGCAATGGTCTGGGTCATGTAAACAACGTTTTTGTCACCCACAGAAG CTTATCTGGATGCCCTCTTAATGCACAAGCTATCAAAAAAGTCAAGGTCTCTGAGGAACTAATGACTATC AAGCTCAAAGCAACTGGGGGTATTGATGGTGATGAAGAAATTAGGCATCTGGATGAAGAAATCAAGGAAC TGAATGAATCCAACCTTAAAATTGAAGCAGATATGATGAAACTTCAGACCCAGATAACATCTATGGAGAG CAACTTGAAGACCATAGAGGAGGAGAACAAGCTTATAGAACAGAGCAATGAGAGTCTGCTGAAGGAGCTG GCAGGGCTTAGCCAGGCTCTCATCTCCAGCCTTGCTGACATCCAACTTCCACAGATGGGGCCTATCAATG AGCAGAATTTTGAAGCATATGTAAATACTCTCACAGACATGTACAGCAATCTGGAACAGGACTATTCCCC AGAATGCAAAGCTCTACTGGAAAGCATCAAGCAGGCGGTGAAGGGCATCCATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231520 representing NM_001244692 Red=Cloning site Green=Tags(s) MDAEVEDKTLHTLSKGTEVPMDSLIPELRVPYDCSMAKKRRAEEQASGVPINKRKSLLMKPRHYSPDMGC KESPDNRNEDDGLLETNDHATADEIMVKSMDETLHLPAQDSSLQKKDQYTCYPELMVKSLVHLGKFEESE SVQTTCENLNGSSIQSLKAESDEAHEGSMVHSDNGRDKVHHSQPPFCSSGDSESDSDSAENGWGNGSNSS EDTDTHKGPKHKLTYNRKDLLEVPEIKAEDDKFIPCENRCDSDTDGRDPQNSHMEPLVVKAQPSFPEVEE GESLATVTEEPAEVEKAKGNLSLLEQAIALQAERGSVFHHTYKELDRFFLDHLARERRQPRVTDANGRQI FTNKHSPRPERREAKCPIPGCDGTGHVTGLYPHHRSLSGCPHKVRVPLEILAMHENVLKCPTPGCTGRGH VNSNRNTHRSLSGCPIAAAEKLAMTQDKSQLDSSQTGQCPEQAHRVNLVKQIEFNFRSHAITSPRASASK EQEKFGKVPFDYASFDAQVFGKRPLLQTGQGQKAPPFPESKHFSNPVKFPNGLPSAGAHTQSTVRASSYG HGQYSEDTHIAAAAAILNLSTRCREATDILSNKPQSLRAKGAEIEVDENGTLDLSMKKNRIHDKSIPPTS SPTTITTPSSSPFNASSLLVNAAFYQALSDQEGWNVPINYSKSHGKTEEEKEKDPVNFLENLEEKKFAGE ASIPSPKPKLHTRDLKKELITCPTPGCDGSGHVTGNYASHRSVSGCPLADKTLKSLMAANSQELKCPTPG CDGSGHVTGNYASHRSLSGCPRARKGGIKMTPTKEEKEDSELRCPVIGCDGQGHISGKYTSHRTASGCPL AAKRQKENPLNGAPLSWKLNKQELPHCPLPGCNGLGHVNNVFVTHRSLSGCPLNAQAIKKVKVSEELMTI KLKATGGIDGDEEIRHLDEEIKELNESNLKIEADMMKLQTQITSMESNLKTIEEENKLIEQSNESLLKEL AGLSQALISSLADIQLPQMGPINEQNFEAYVNTLTDMYSNLEQDYSPECKALLESIKQAVKGIHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244692
ORF Size	3135 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244692.1, NP_001231621.1
RefSeq Size	5913 bp
RefSeq ORF	3138 bp
Locus ID	240690
UniProt ID	Q80TY4
MW	114.8 kDa
Gene Summary	Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.