Epb41l2 (NM_001199265) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4.1G; AW555191; D10Ertd398e; Epb4.1l2; NBL2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231461 representing NM_001199265 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACTGAAGTTGGCTCTGCATCTGAAGTGAAGAAGGGCTCTGACCAGGCTGGAGCAGATGCCTCCA AGGAGAAAGCTAAAGAAGTAGAAAATGAGCAGACTCCAGTATCTGAGCCAGAGGAAGAAAAGGGTTCCCA GCCAGGTCCTCCGGTTGAGAGGCAAAGCACTCCCCGCCTGCGGAAACGAGGGAAGGATCCGTCTGAGAAC AGGGGCATCTCTCGATTCATACCCCCGTGGCTTAAGAAGCAGAGGTCTTACAATTTAGTAGTAGCCAAAG ATGGAGGAGATAAAAAAGAGCCTACTCAAGCTGACGTGGAAGACCAGATCTTAGGCAAAGAGGAATCCCT TCCTGAAGAAGAGAGTCGGGCCAAGGGTGACGCAGAAGAAATGGCTCAGAGGAAGCACCTGGAGGTTCAG GTGGAGGTCAGGGAAGCAAAACCAGCCTTGAAAAGCAGCGTGGAGACCCAGCCTGCTGAAGAAGTGAGGA AGGACAAGGAGGAAACGATACAAGACACACAGGAAGAGAAACTGGAAGGAGGTGCAGCCAAAAGGGAGAC AAAGGAAGTACAGACAAGTGAGCTGAAGGCAGAGGTGGCCTCTCAGAAAGCTACCAAGAAGACCAAGACT GTCCTGGCCAAAGTGACCCTTCTAGATGGCACGGAATACAGCTGTGACCTTGAGAAACGTGCCAAGGGAC AAGTGTTGTTTGACCGAGTGTGCGAGCACCTGAACCTCTTGGAGAAGGACTACTTTGGACTTCTATTTCA GGATCATCCTGAGCAGAAAAACTGGCTAGATCCTGCAAAGGAAATAAAGAGACAGCTGAAAAATCTTCCC TGGCTGTTCACTTTCAATGTGAAATTTTATCCTCCAGATCCTTCTCAGCTGACTGAAGATATCACCAGAT ACTTCTTGTGCCTTCAGCTAAGGCAGGACATCGCCTCTGGCCGCCTGCCCTGCTCCTTTGTGACTCACGC CCTCTTGGGATCCTACACCTTGCAGGCCGAGCATGGGGACTATGACCCAGAAGAATATGACAGTATTGAT CTTGGTGACTTCCAGTTTGCCCCGGCCCACACCAAGGAGTTGGAGGAAAAGGTGTCAGAACTGCATAAAA CTCACAGGGGCTTATCTCCAGCACAAGCCGACTCTCAGTTCTTAGAGAACGCAAAGAGGCTTTCCATGTA CGGTGTTGACCTGCACCATGCCAAGGACTCTGAAGGTGTGGACATCAAGCTGGGTGTGTGTGCCAATGGC CTTCTCATTTACAAAGACAGACTAAGGATCAATCGTTTTGCTTGGCCGAAGATTTTGAAAATTTCCTATA AGCGCAGTAATTTCTACATTAAAGTTAGACCAGCAGAGCTGGAACAGTTTGAGAGTACCATTGGATTCAA ATTGCCAAACCACCGGGCAGCTAAACGGCTATGGAAGGTGTGTGTGGAACATCACACTTTCTACAGGCTT GTGTCTCCAGAGCAGCCACCAAAGACCAAGTTCTTGACCCTGGGGTCCAAATTCCGCTACAGCGGCCGCA CGCAGGCACAGACCAGAGAAGCAAGTACCCTCATAGACAGGCCGGCACCACAATTTGAGCGTGCCTCTAG TAAACGTGTCTCGAGGAGCCTAGACGGAGCCCCGATTGGTGTTGTGGACCAAAGCCCTCCTGGAGAGGGC TCAGTGCCTGGCCCTGGAGTTATCAGCTATACTACCATCCAGGATGGCCGGAGGGACAGCAAAAGCCCAA CAAAAGCTACACCCCTGCCCGCTGAGGGAAAGAAAAATACCTTGAGAGTAGACGGGGATAATATTTATGT CAGACATAGCAATTTAATGTTGGAGGACCTGGATAAGGCCCAGGAGGCCATTCTGAAACATCAGGCTAGC ATTAGTGAACTCAAGCGCAATTTCATGGCGTCCACACCTGAGCCTCGCCCCAGTGAATGGGAGAAGCGGC GGGTCACACCTCTGCCTTTCCAACCACAAGCGAGTTCACATGAGACTCTGAATGTAGTGGAGGAGAAGAA GCGGGCAGAGGTTGGGAAAGACGAAAGCGTAATCACAGAAGAAATGAATGGTAAAGAGATGTCACCGGGG CATGGTCCTGGGGAGACTCGTAAGGTGGAGCCTGTGGCACACAAAGACTCCACCTCCCTGTCTTCTGAGA GCAGCAGCAGCAGCAGTGAGAGTGAGGAGGACGTGGGAGAGTACCAGCCCCACCACCGAGTGACCGAGGG CACCATAAGGGAAGAGCAGGAGGAGTGTGATGAAGAGCTGGAGGAAGAGCCCGGCCAAGGAGCCAAGGTA GTAGAGAGGGAGGCAGCAGTGCCCGACGCCGTCCCAGACAGACAAGCAGGGGCCAGTGTGCTCCCAGTAG AAACAGAGGCCCAGGAACATGTAGTTGCCCAAAAGTTACCTGGAGAAAAGGGTGCACACGGAGGCACTGC TGAGCAGGACCCGAGAGAAGAAGCAGAGGAAGACCCGCACAGAGTTAATGGAGAGGTGCCCCATCTTGAC CTTGATGGTTTGCCAGAGATTATTTGTTGTTCAGAGCCACCAGTGGTAAAAACAGAGATGGTAACAATTT CTGATGCCTCACAAAGGACAGAAATCTCCACCAAGGAAGTCCCCATTGTTCAAACTGAGACCAAAACCAT CACATATGAGTCTCCACAGATTGACGGCGGGGCTGGTGGTGATTCGGGCGTGTTACTGACCGCACAAACC ATCACATCTGAGTCCGCGTCAACAACGACAACCACACACATCACCAAGACTGTGAAAGGTGGGATCTCTG AAACACGAATTGAGAAACGCATTGTGATCACAGGAGACGCAGCTCTGGATCATGATCAGGCGCTGGCTCA GGCCATACGGGAAGCGAGAGAGCAGCACCCTGATATGTCGGTCACAAGGGTGGTAGTGCACAAAGAAACA GAGTTGGCGGAGGAAGGAGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231461 representing NM_001199265 Red=Cloning site Green=Tags(s) MTTEVGSASEVKKGSDQAGADASKEKAKEVENEQTPVSEPEEEKGSQPGPPVERQSTPRLRKRGKDPSEN RGISRFIPPWLKKQRSYNLVVAKDGGDKKEPTQADVEDQILGKEESLPEEESRAKGDAEEMAQRKHLEVQ VEVREAKPALKSSVETQPAEEVRKDKEETIQDTQEEKLEGGAAKRETKEVQTSELKAEVASQKATKKTKT VLAKVTLLDGTEYSCDLEKRAKGQVLFDRVCEHLNLLEKDYFGLLFQDHPEQKNWLDPAKEIKRQLKNLP WLFTFNVKFYPPDPSQLTEDITRYFLCLQLRQDIASGRLPCSFVTHALLGSYTLQAEHGDYDPEEYDSID LGDFQFAPAHTKELEEKVSELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANG LLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRL VSPEQPPKTKFLTLGSKFRYSGRTQAQTREASTLIDRPAPQFERASSKRVSRSLDGAPIGVVDQSPPGEG SVPGPGVISYTTIQDGRRDSKSPTKATPLPAEGKKNTLRVDGDNIYVRHSNLMLEDLDKAQEAILKHQAS ISELKRNFMASTPEPRPSEWEKRRVTPLPFQPQASSHETLNVVEEKKRAEVGKDESVITEEMNGKEMSPG HGPGETRKVEPVAHKDSTSLSSESSSSSSESEEDVGEYQPHHRVTEGTIREEQEECDEELEEEPGQGAKV VEREAAVPDAVPDRQAGASVLPVETEAQEHVVAQKLPGEKGAHGGTAEQDPREEAEEDPHRVNGEVPHLD LDGLPEIICCSEPPVVKTEMVTISDASQRTEISTKEVPIVQTETKTITYESPQIDGGAGGDSGVLLTAQT ITSESASTTTTTHITKTVKGGISETRIEKRIVITGDAALDHDQALAQAIREAREQHPDMSVTRVVVHKET ELAEEGEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199265
ORF Size	2964 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199265.1, NP_001186194.1
RefSeq Size	4389 bp
RefSeq ORF	2967 bp
Locus ID	13822
UniProt ID	O70318
MW	109.9 kDa
Gene Summary	Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.