Sez6l (NM_001253916) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Acig1; AI843918; AIG1; AW539724; BSRP-B; mKIAA0927
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231435 representing NM_001253916 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGTGGCACGGCCACAAGCCGCGGGACCCGACAGGATCTCTCTGTTCCTTGTCGCCTTCCTTCTGG GGAGCCCCGCGGCAGCTCAGGCGGAAGATGGTGGTCCAGAGGGAGAGATGCACCCTTCCACTGCCTACCT CCTGCCCTCTGCCTCGCTGGAGAGTAGCCTGGAAGAGGGGGTGACATCAGCAGCCCCAGGTCTCCTGCCA TCCCAAGAAGCACTGGAAGCAATGGAAGAGTCCTTGCCCCCTGCGCTGCCCGACGAGGCCAGTGTCCAGC ACACGCCTGCCCTAAGGAAGGGTCTGCCGTCCCTGAAGCAACTCAACTCGGCCCGGAGGCAGCTGAGGCC CCTGGCTACCCCGACAACTCTGCAGAGACTGGGGTCTCCTGCCTCTGCCACCACGAAGCTGCGTGAGCCT GAAGACCCCGAGCAGCCCACGGCGCCTGCGCCACTGCAGATCGCGCCCTTCACTGCACTGGCGACCACAC TCCCACACAGCCCTCAGCCCGCTCAGGCCCCTGATGACAGCAGCCCCGGTAGCCCGCTGGACAAGGGGGA CAATGAGCTGACCGGGTCTGCCTCCGAGGAGAGCCAGGAGACCACCACGTCCACCATCGTCACTACCACC ATCATCACCACGGAGCAGGCCCCAGCTCTCTGCGGTGTGAGCTTCTCCGACCCGGAGGGATACATTGACT CAAGTGATTTCCCTCCACAACCCTACAGCAGCTTCCTGGAATGCACTTACAATGTGACGGTCTACACTGG CTACGGGGTGGAACTGCAGGTCAAGAGTGTCAACCTCTCCGAGGGGGAGCTGCTCTCCATCCGAGGAGTG GACGGTCCCACTCTGACAGTCCTGGCCAACCAGACTCTACTGGTAGAGGGCCAGGTGATCCGCAGCCCCA CCAACACCATCTCTGTCTACTTCCGGACCTTCCAGGATGATGGCCTCGGGACCTTCCAGCTACATTACCA GGCCTTCATGCTGAGCTGCCCCTTTCCCCGGAGGCCCGATGCCGGAGAGGTCACGGTGATGGATCTGCAC TCGGGCGGGGTGGCCCACTTCCATTGCCACCTGGGCTACGAGCTGCAGGGAGCCAAGACGCTGACCTGCA TCAATGCCTCCAAACCGCACTGGAGCAGCCAGGAGCCTGTCTGCTCAGCCCCATGTGGAGGAGCAGTGCA CAATGCCACCATCGGCCGAGTTCTCTCCCCCAGTTTCCCGGGAACCGCCAATGGCAGCCAGCTCTGCGTG TGGACCATAGAGGCTCCAGAGGGTCAGAAGCTTCACCTGCACCTTGAAAGGCTGCTGCTGCATGAGAAAG ACAGGATGATTGTCTACAGTGGAAGAACAAACACATCTGCCCTCCTGTACGACTCTCTCAGAACAGAGAG CGTGCCCTTCGAAGGCCTGCTGAGCGAGGGTAGCAGTATCCGCATTGAGTTCACGTCGGACCAGGGCCAG GCAGCTTCCGCCTTCAACATCCGCTTTGAGGCCTTTGAGAAAGGTCACTGCTATGAACCCTACATCCAAA ATGGGAATTTCACCACATCGGACCCCACCTACAACATCGGCACCATTGTGGAGTTCACCTGCGACCCCGG CCACTCTCTGGAACAGGGCCCGGCTGTCATCGAGTGTGTCAATGTGCGTGACCCATACTGGAATGACACA GAGCCTCTATGCAGAGCCATGTGTGGTGGGGAGCTCTCTGCTGTGGCTGGGGTGGTGCTGTCTCCAAACT GGCCAGAGCCCTATGCAGAAGGTGAAGACTGTGTGTGGAAGATCCACGTTGGGGAGGAGAAGCGGATCTT CCTGGACATTCAGTTCCTTAACCTGAGCAACAGCGACATCCTGACCATCTATGATGGCGATGAGGTTGTG CCCCACGTCCTGGGCCAGTACTTTGGCCACAGCAGCCCTCAGAAGCTGTACTCCTCCACACCGGACCTCA CCATCCAGTTCCACTCCGACCCTGCAGGCCTCATCTTTGGCAAGGGCCAGGGATTTATCATGAACTATAT AGAGGTGTCACGGAACGACTCCTGCTCAGACTTGCCTGAAATTCAGAACGGCTGGAAAACTACTTCCCAC ACAGAGCTGGTCAGGGGAGCCCGGATCACATACCAGTGTGACCCAGGCTATGACATTGTGGGGAGTGACA CCCTCACCTGCCAATGGGACCTCAGTTGGAGCAGCGACCCGCCGTTTTGTGAAAAAATTATGTACTGTAC TGACCCCGGGGAGGTGGAGCACTCCACCCGCCTCATTTCAGACCCTGTGCTGCTAGTGGGCACCACCATC CAGTACACCTGTAGCCCTGGCTTTGTGCTTGAAGGGAGCTCTCTTCTCACCTGCTACAGCCGCGAGACAG GAACTCCCATCTGGACATCTCGCCTGCCCCACTGTGTCTCTGAGGAGTCCCTGGCGTGTGACAACCCAGG ACTGCCTGAGAACGGGTACCAGATCCTGTACAAGCGGCTGTACCTGCCCGGAGAGTCCCTCACCTTCATG TGCTACGAGGGCTTTGAGCTCATGGGCGAAGTGACCATCCGGTGCATTCTGGGACAGCCGTCCCATTGGA GTGGGCCCCTGCCCATCTGTAAAGTTAACCAAGACAGTTTTGAACATGCATTGGAAGCAGAAGCGGCAGC AGAGTCCTCACTGGAAGGTGGGAACATGGCGCTGGCCATCTTCATTCCGGTCCTTCTCATCTCCTTGCTA CTGGGAGGAGCCTACATCTACGTCACGAGGTGCCGACAATACTCCAGCCTCCGCCTACCCCTCATGTACT CCCACCCCTACAGTCAGATCACCGTGGAGACTGAGTTCGACAATCCCATCTACGAGACTGGGGAAACAAG AGAGTATGAAGTTTCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231435 representing NM_001253916 Red=Cloning site Green=Tags(s) MPVARPQAAGPDRISLFLVAFLLGSPAAAQAEDGGPEGEMHPSTAYLLPSASLESSLEEGVTSAAPGLLP SQEALEAMEESLPPALPDEASVQHTPALRKGLPSLKQLNSARRQLRPLATPTTLQRLGSPASATTKLREP EDPEQPTAPAPLQIAPFTALATTLPHSPQPAQAPDDSSPGSPLDKGDNELTGSASEESQETTTSTIVTTT IITTEQAPALCGVSFSDPEGYIDSSDFPPQPYSSFLECTYNVTVYTGYGVELQVKSVNLSEGELLSIRGV DGPTLTVLANQTLLVEGQVIRSPTNTISVYFRTFQDDGLGTFQLHYQAFMLSCPFPRRPDAGEVTVMDLH SGGVAHFHCHLGYELQGAKTLTCINASKPHWSSQEPVCSAPCGGAVHNATIGRVLSPSFPGTANGSQLCV WTIEAPEGQKLHLHLERLLLHEKDRMIVYSGRTNTSALLYDSLRTESVPFEGLLSEGSSIRIEFTSDQGQ AASAFNIRFEAFEKGHCYEPYIQNGNFTTSDPTYNIGTIVEFTCDPGHSLEQGPAVIECVNVRDPYWNDT EPLCRAMCGGELSAVAGVVLSPNWPEPYAEGEDCVWKIHVGEEKRIFLDIQFLNLSNSDILTIYDGDEVV PHVLGQYFGHSSPQKLYSSTPDLTIQFHSDPAGLIFGKGQGFIMNYIEVSRNDSCSDLPEIQNGWKTTSH TELVRGARITYQCDPGYDIVGSDTLTCQWDLSWSSDPPFCEKIMYCTDPGEVEHSTRLISDPVLLVGTTI QYTCSPGFVLEGSSLLTCYSRETGTPIWTSRLPHCVSEESLACDNPGLPENGYQILYKRLYLPGESLTFM CYEGFELMGEVTIRCILGQPSHWSGPLPICKVNQDSFEHALEAEAAAESSLEGGNMALAIFIPVLLISLL LGGAYIYVTRCRQYSSLRLPLMYSHPYSQITVETEFDNPIYETGETREYEVSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253916
ORF Size	2889 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253916.1, NP_001240845.1
RefSeq Size	6098 bp
RefSeq ORF	2892 bp
Locus ID	56747
UniProt ID	Q6P1D5
MW	105.3 kDa
Gene Summary	Candidate tumor suppressor gene. May contribute to specialized endoplasmic reticulum functions in neurons.[UniProtKB/Swiss-Prot Function]

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