Tdrd5 (NM_001277730) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm103
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231411 representing NM_001277730 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAACAAGAGCGTATACAGGACTGTCTGAGGAAAGAGATAAGGTCCCTTCTTATTTCCACTAAGG ATGGTTTGACTCCACAACAGTTGGAAAAGGAGTACCTTTTGATGGTTGGCAACCATCTCCCGCTCCGAAT CCTTGGATATCGGTCCACTATGGAACTGGTGTTGGATATGCCCGATGTGGTTAGTGTCTGCCCCGGTGGG GATGGTACCGTAATACTGAAAGCCATTCCAGACGAATCCACCAAAGGAATAGCAAGTTTGGTTGCAAAGC AGAGAAGGAGCCATAAGGTCCGAAACTCCATGCCAAAGGGAAGACCCAGCATATGCTCTGGCCGGGTGCC TTACCGAGGAAGGGTGCCCCCAATTCTTCCAGCTGTAGTGAAGAGATTCCCAGTAATGAAGGCACACTTT TCACAACCCATTTCAAATATGGAGCCCCCCAAACAAGTACTGAGCATGGCGAAGACACCCACGTTTAATT CTGTGGAGGCTTCAAGGCTGAACCACACTGAAAAATTAAATCAGTTGGAGAGCACATTCAAATCCGTGAT TGCACAGATTGGACCTGGAGGGACTGTTGATCCTGAGCTAAAGCGCAAGATACAATTTGTCGTGTCTAAG TTTCCACAAGGTTTGTTTATTTCTAAACTGCTTGGAGAATTTGAGCTAACTTTTAAAGAGCAACTTTCAC CAAAACAATTAGGGTTCTTAAACGTGACAGAACTTGTTGGCGCTCTTAGTGACATTCTCCGAGTTGAGTT CAGTGAAGAAAGGCAAGACTTGCTCGTGTTTGATGCTGACCTGAAGCCTCTGCCGTCTGGTGGACCGCTT TCATCAGTCAGAAATTCGTGTTTAGTTCAACCAGACAAGAAAACAGAAGCTAACCCTTGGACTTCCAGTC TCTCCAGAAATTCACTATCCACTGTAGCAGTTAAGAAGACCACGTGGGATTGCCCTTTGAAGAACCAGAA GGAGCCAGAACAGAAGATATACAAGAAGCCTAATCTGGTGGTCAAGCCTCTGCAGCTGCAGGTAGAAACA AACAAACCGCAGCTCAGCCTGTCAGTGGCAAACCACGATATCCCACCAGACGCTGTGCGTGCCAAGAAAT TGTGCAGACTTCCTCCATTAGATACCAGCACCCTTGTGGGGGTCTTCGTGGAGTATATAATCTCCCCTAG TCAGTTCTACATCCGGATCTACAGCAGAGATTCCTCAGAACTACTTGAAGACATGATGATTGAAATGCGA CGCTGCTACTCCAACCAGCTGGTCTCTGACCGGTACATTATGCCAGAATATTTTATACAACCAGGACATC TCTGTTGTGTAAAGATCTCTGAGGACAAGTGGTGGTACCGGGTTATTATCCATCGGATCCTTGGGAAAAA GGAAGTGGAAGTGTTCTACCCAGATTTCGGAAATATCGGAACTGTGCAGAAGTCCTCGCTGAGGTTCCTC AAATGCTGCTACACAAAGCTTCCAGCTCAGGCTATCCCTTGCTCTTTGGCGTGGGTGAGGCCCGCAGAGG AACACTGGACAGCCAGAGCTATTTTGCACTTCCAGAAGCTGTGTGGTTTGAAGCCATTGGTGGGGGTGGT GGATGAGTATATAGACGGAATCCTTAATATTTTTCTTTGTGATACGTCCTCAAATGAAGATGTGTATTTT CATCATGTCTTGAGGACAGAAGGCCATGCTATTGTATGCCGAGAAAATGCCCCATCCAAGGGCTTCAGGG ATTTCAACCCACCAGCTCTGTACACTAACGCCAGCTCAGCGGGCGACATGGTCCTGACAGACCTGGGGCA TCCTGCTCAGCAGCACTATTTAAATGAAGACCAGGAGATACTGCAGCAAGCCCAGCAAGATATTAATGAT GGAAAGTGTATAAGTTACCTTAAATCTGCGCCCAAGGAATTATTAAAGGATTCTAAATTGAGCTCATTGA AAACACATAAGAGCTGTGAAGAGGACCCACGGTGGTCCATCCTGCAGCCCAAGGATCTGAAGGAAGAAAA TGAGGATGAGGTCCCTACTGGAATGCCATGCCTGGAGTCAGTGACCATAGGTGACGATGTTTGGGATGAG AACTGGCTCCCTCTACAGGCCAAAATGGGGAAGGCAGGGAGTCCTGCCTCCCAGCTGTTTACTTCAAACC TTGTTGGGAAGAAACAATATCAGACAGGTGGAGAAATGGCACAAAAGGACTGGTGTTTTTCTACATCTAA AGATATATGGGATGATTCCTGGCAGCCCTTAGGCCTTGCGAATGATGTGAAAGGAGGAATTCACACACCA GAAGGGCCCATTGCTCAAGAAAAAAATACCAGCACAACCCGAATTCAACAGCAGCCAGATTTACAGTATC CTTTGGATTCTTCCACACTGCCCAAACTGGAAGAGTTCTACATCTCTCTGATCAAGTCTCAGCAGTCCGC AGAGAGGAGCCAGTCTGAACCTGCCAGCATTCAGACTCATGCCGGACGGGCAGCCTCAAAAGCACTCAGT TCAACCCCTGCAGTGGGTGACTCTCCAGAAAACCACTCTGGCTCTGTGGAAAGCTCTCCAGGGAGCCTAA AGAAAGAAGATGTTTCTAATAGCCGTGCTGAAGCCACGGCCAAGGACAAGAGTCAGGGAGCCATAGACCA GCTCTCTTTCATTTTGTCCCCCCAGCACCAGATTTCTCAGAAGCTCTACATCCCTCGAAGTACAGCCACA GCTGTCTTGGGAGCTGCGGCACGGTTAGCCACGTCCAGGAGCCTCCTGCACTGGTACCCCAGTGTGAAAG GAGGGAAGCTTGAGGCAGAGAGAGATGGAGTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231411 representing NM_001277730 Red=Cloning site Green=Tags(s) MSEQERIQDCLRKEIRSLLISTKDGLTPQQLEKEYLLMVGNHLPLRILGYRSTMELVLDMPDVVSVCPGG DGTVILKAIPDESTKGIASLVAKQRRSHKVRNSMPKGRPSICSGRVPYRGRVPPILPAVVKRFPVMKAHF SQPISNMEPPKQVLSMAKTPTFNSVEASRLNHTEKLNQLESTFKSVIAQIGPGGTVDPELKRKIQFVVSK FPQGLFISKLLGEFELTFKEQLSPKQLGFLNVTELVGALSDILRVEFSEERQDLLVFDADLKPLPSGGPL SSVRNSCLVQPDKKTEANPWTSSLSRNSLSTVAVKKTTWDCPLKNQKEPEQKIYKKPNLVVKPLQLQVET NKPQLSLSVANHDIPPDAVRAKKLCRLPPLDTSTLVGVFVEYIISPSQFYIRIYSRDSSELLEDMMIEMR RCYSNQLVSDRYIMPEYFIQPGHLCCVKISEDKWWYRVIIHRILGKKEVEVFYPDFGNIGTVQKSSLRFL KCCYTKLPAQAIPCSLAWVRPAEEHWTARAILHFQKLCGLKPLVGVVDEYIDGILNIFLCDTSSNEDVYF HHVLRTEGHAIVCRENAPSKGFRDFNPPALYTNASSAGDMVLTDLGHPAQQHYLNEDQEILQQAQQDIND GKCISYLKSAPKELLKDSKLSSLKTHKSCEEDPRWSILQPKDLKEENEDEVPTGMPCLESVTIGDDVWDE NWLPLQAKMGKAGSPASQLFTSNLVGKKQYQTGGEMAQKDWCFSTSKDIWDDSWQPLGLANDVKGGIHTP EGPIAQEKNTSTTRIQQQPDLQYPLDSSTLPKLEEFYISLIKSQQSAERSQSEPASIQTHAGRAASKALS STPAVGDSPENHSGSVESSPGSLKKEDVSNSRAEATAKDKSQGAIDQLSFILSPQHQISQKLYIPRSTAT AVLGAAARLATSRSLLHWYPSVKGGKLEAERDGVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277730
ORF Size	2835 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277730.1, NP_001264659.1
RefSeq Size	3168 bp
RefSeq ORF	2838 bp
Locus ID	214575
UniProt ID	Q5VCS6
MW	105.8 kDa
Gene Summary	Required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Probably acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for chromatoid body (CB) assembly.[UniProtKB/Swiss-Prot Function]

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