Pde2a (NM_001243758) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CGS-PDE; cGSPDE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231410 representing NM_001243758 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGGGCTTGCACCCTTTTCAGCCTCCTGGGACGCCGATGAAGGCCTATCTGGTAGCCTTAGGA ACCCAGTGCTCTCACCCCACATTTGGGAACGGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAGCCGCC GCCGCAGCCATGCGCCGACAGCCTGCAGGATGCTTTGCTGAGCCTAGGCGCCGTTATCGACATTGCTGGC CTGCGACAGGCTGCCAGGGATGCCCTCTCAGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGC TAGATGGGGAGTCCAGACTGGTGTGTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGA AGCTGTGATCTCTCAGAAGCGGCTGAGTTGCAATGGGCTGGGACCTTCGGACCTACTGGGAAAGCCCTTG GCCAGGCTGGTGGCTCCACTGGCTCCTGACATGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTG GAAGTGTGGCAGCTGTCATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTGAGGAACAGAGCTTGCAGGT GGTAGAGAAGCACGCTCTGGTAGCCCTGCGGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTT CAGAACACGTCAGTGGATGCCTCTGAAGATCAAAAGGATGAGAAGGGGTACACCGACCATGACCGAAAGA TCCTGCAACTGTGTGGGGAACTCTTTGACTTGGATGCTACTTCTCTGCAGCTCAAAGTCCTTCAATATCT GCAGCAGGAGACACAGGCCACTCACTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTACAGCTTTCCTGC AAGGTCATTGGAGACAAAGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGTCTGGGGCAGG TGGTGGAAGACAAGCAGTGTATCCAGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTGCAAAACAT GTTGGGTTGTGAGCTGCAGGCTATGCTGTGTGTCCCTGTCATCAGCCGAGCCACTGACCAGGTGGTGGCC CTGGCTTGCGCCTTCAACAAGCTTGGAGGAGATTTCTTCACAGATGAGGATGAACACGTGATCCAACACT GCTTCCACTACACAGGCACGGTGCTCACCAGCACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGA GTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGATGTCTCTGTCCTGCTACAG GAGATCATCACGGAAGCCAGAAACCTCAGCAACGCAGAGATCTGCTCGGTGTTCCTGCTGGATCAGAACG AGCTGGTGGCCAAGGTGTTCGATGGTGGCGTTGTGGACGATGAGAGTTATGAGATCCGCATCCCGGCGGA CCAAGGCATCGCGGGCCACGTGGCGACTACGGGCCAGATCCTGAACATCCCAGATGCATATGCCCATCCG CTTTTCTATCGCGGCGTAGATGACAGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGA ACGAGAACCAGGAGGTCATTGGTGTGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAGTT TGATGAGGACCTGGCCACAGCCTTCTCCATCTACTGTGGCATCAGCATCGCCCACTCTCTCCTATACAAA AAGGTGAATGAAGCCCAATACCGCAGCCACCTGGCCAATGAGATGATGATGTATCATATGAAGGTCTCTG ATGATGAATACACCAAGCTTCTCCACGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAA CTTTACCTACACGCCTCGGTCTCTGCCTGAGGACGACACTTCTATGGCCATCCTGAGCATGCTGCAAGAC ATGAACTTCATCAATAACTACAAAATTGACTGCCCAACTCTGGCCCGATTCTGCCTGATGGTGAAGAAAG GCTACCGGGATCCACCCTACCACAACTGGATGCACGCCTTCTCTGTCTCTCATTTTTGCTACCTGCTCTA CAAGAATCTGGAGCTCTCCAACTACCTCGAGGACATCGAGATCTTTGCATTGTTTATTTCCTGCATGTGT CATGACCTGGACCACAGAGGCACAAACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCCGCACTCT ACAGCTCAGAGGGCTCTGTCATGGAGAGGCACCACTTTGCTCAAGCCATTGCTATCCTCAACACCCACGG CTGCAATATCTTTGACCACTTCTCTCGGAAGGACTATCAGCGCATGCTGGACCTGATGAGGGACATCATC TTGGCTACAGACCTGGCACACCACCTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATG ACCGAAACAACAGGCAACACCACAGGCTTCTTCTGTGCCTCCTCATGACCTCCTGTGACCTCTCTGACCA GACAAAGGGCTGGAAGACCACCAGAAAGATTGCAGAGCTGATCTACAAAGAGTTCTTCTCCCAGGGAGAC TTGGAGAAGGCCATGGGCAACCGACCGATGGAGATGATGGACCGTGAGAAGGCCTACATCCCTGAGCTTC AGATCAGCTTTATGGAGCACATTGCCATGCCTATCTACAAGCTTTTACAAGACCTGTTCCCCAAGGCGGC AGAGCTGTATGAACGTGTGGCCTCCAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGA GGCCTCCCCAGTAACAACTCGCTGGATTTCCTGGACGAGGAATACGAGGTCCCCGATTTGGACGGCACCA GAGCTCCTGTCAATGGCTGCTGCAGCCTCGAGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231410 representing NM_001243758 Red=Cloning site Green=Tags(s) MALGLAPFSASWDADEGLSGSLRNPVLSPHIWERRGQQVFLKPDEPPPQPCADSLQDALLSLGAVIDIAG LRQAARDALSAVLPKVETVYTYLLDGESRLVCEDPPHELPQEGKIREAVISQKRLSCNGLGPSDLLGKPL ARLVAPLAPDMQVLVIPLLDKETGSVAAVILVHCGQLSDSEEQSLQVVEKHALVALRRVQALQQRRPEAV QNTSVDASEDQKDEKGYTDHDRKILQLCGELFDLDATSLQLKVLQYLQQETQATHCCLLLVSEDNLQLSC KVIGDKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELQAMLCVPVISRATDQVVA LACAFNKLGGDFFTDEDEHVIQHCFHYTGTVLTSTLAFQKEQKLKCECQALLQVAKNLFTHLDDVSVLLQ EIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHP LFYRGVDDSTGFRTRNILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYK KVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAILSMLQD MNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELSNYLEDIEIFALFISCMC HDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDII LATDLAHHLRIFKDLQKMAEVGYDRNNRQHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGD LEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIR GLPSNNSLDFLDEEYEVPDLDGTRAPVNGCCSLEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243758
ORF Size	2835 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243758.1, NP_001230687.1
RefSeq Size	4112 bp
RefSeq ORF	2838 bp
Locus ID	207728
MW	106.8 kDa
Gene Summary	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.[UniProtKB/Swiss-Prot Function]

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