Ganab (NM_001293621) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU042638; G2an; GluII; mKIAA0088
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231409 representing NM_001293621 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAATAGCGGCAGTGGCGGCGCGTAGGAGGCGGTCTTGGTTAAGTTTGGTGCTGGCATACTTAG GGGTCTGTCTGGGGATTACACTTGCTGTGGATAGAAGCAACTTTAAGACCTGTGATGAGAGTTCCTTTTG CAAACGGCAGCGAAGCATTCGGCCAGGCCTCTCTCCTTACCGTGCCTTGCTGGACACTCTGCAGCTTGGT CCTGATGCTCTTACAGTCCATCTGATCCATGAAGTCACCAAGGTGCTGCTTGTGCTGGAGCTCCAGGGCC TTCAGAAGAACATGACTCGGATCAGGATCGATGAGCTAGAGCCCCGGCGGCCTCGATACCGAGTGCCAGA TGTTTTAGTGGCTGACCCCCCCACAGCTAGGCTTTCAGTCTCTGGCCGTGATGACAACAGTGTGGAGCTA ACAGTGGCTGAGGGACCCTACAAAATCATTTTGACAGCACAGCCATTCCGCCTTGACCTGCTAGAAGATC GAAGCCTCCTGCTCAGTGTCAATGCCCGAGGACTTATGGCCTTTGAGCACCAGAGGGCCCCCAGGGTCCC GCAAGAATCAAAAGACCCAGCTGAAGGCAATGGAGCCCAGCCTGAAGCAACACCTGGGGATGGTGACAAG CCAGAGGAGACCCAGGAAAAGGCTGAGAAGGATGAGCCAGGAGCCTGGGAGGAGACATTCAAAACACATT CTGACAGCAAGCCTTATGGCCCCACGTCTGTAGGTTTGGACTTTTCTCTGCCAGGAATGGAACATGTGTA TGGGATCCCTGAGCATGCTGACAGCCTGAGACTGAAGGTCACTGAGGGCGGTGAGCCGTACCGCCTGTAC AATTTGGATGTGTTCCAGTATGAGCTGAACAACCCCATGGCTCTATATGGGTCTGTGCCTGTGCTCCTGG CACACAGCTTTCATCGAGACCTGGGCATCTTCTGGCTTAATGCTGCTGAGACTTGGGTTGATATATCCTC CAACACGGCTGGGAAGACCCTGTTTGGGAAGATGCTTGATTACCTGCAGGGCTCTGGGGAGACTCCACAG ACAGACATTCGTTGGATGTCAGAGAGTGGCATTATTGATGTTTTCCTAATGCTTGGCCCTTCGGTTTTTG ATGTCTTTAGGCAGTATGCTAGTCTCACAGGGACCCAGGCATTGCCCCCACTCTTCTCCCTCGGCTATCA CCAGAGTCGCTGGAACTACCGGGATGAGGCTGATGTTTTGGAAGTGGATCAGGGTTTTGATGATCACAAC ATGCCTTGTGATGTCATTTGGTTGGACATTGAACATGCTGATGGCAAGCGGTACTTCACTTGGGACCCCA CCCGATTTCCTCAGCCCCTCAATATGCTTGAGCACTTGGCTTCCAAGAGGCGGAAGCTGGTGGCCATTGT GGACCCCCACATCAAGGTAGACTCTGGCTACCGAGTTCACGAAGAATTGCGAAACCATGGGCTGTATGTT AAAACTCGGGATGGCTCTGATTACGAGGGCTGGTGCTGGCCAGGCTCAGCTAGTTACCCTGACTTCACTA ATCCAAGGATGAGGGCCTGGTGGTCTAACATGTTCAGCTTTGACAATTATGAGGGTTCAGCTCCTAATCT TTATGTTTGGAATGACATGAATGAACCGTCTGTGTTCAATGGTCCTGAGGTCACCATGTTGAAGGATGCT GTGCATTATGGAGGCTGGGAGCACCGGGACATCCATAACATCTATGGCTTATATGTGCACATGGCGACTG CTGATGGGCTAATACAGCGCTCTGGGGGCATAGAGCGTCCCTTTGTCCTGAGTAGGGCTTTCTTCTCAGG CTCCCAGCGCTTTGGAGCTGTGTGGACAGGGGACAACACTGCCGAATGGGATCATTTGAAGATCTCTATC CCTATGTGTCTCAGCCTGGCACTGGTGGGGCTTTCCTTCTGTGGAGCGGATGTGGGTGGCTTCTTCAAGA ACCCAGAGCCAGAGCTGCTTGTGCGCTGGTACCAAATGGGTGCCTACCAGCCGTTCTTTCGGGCTCATGC CCACTTGGACACTGGGCGGCGAGAGCCATGGCTGTTAGCGTCTCAATACCAAGATGCAATCCGAGATGCC TTGTTCCAGCGATATTCTTTGCTGCCCTTCTGGTATACCCTCTTCTATCAAGCTCACAAGGAAGGGTTTC CTGTCATGAGGCCCCTCTGGGTACAGTATCCTGAGGATATGTCTACCTTCAGTATAGAGGATCAGTTCAT GCTTGGTGATGCACTCCTTATTCACCCTGTATCGGATGCTGGGGCCCACGGAGTGCAGGTCTATTTGCCT GGCCAAGAAGAAGTGTGGTATGACATTCAGAGCTATCAGAAGCATCATGGGCCCCAGACCTTGTATCTGC CAGTAACTTTGAGCAGTATCCCCGTGTTCCAGCGTGGCGGAACCATTGTGCCTCGATGGATGCGTGTGAG GCGCTCTTCAGACTGTATGAAGGACGATCCTATCACTCTCTTTGTTGCTCTCAGTCCCCAGGGTACTGCC CAAGGAGAGCTCTTTCTAGATGATGGACACACCTTTAACTATCAGACTCGCCATGAGTTCCTGTTGCGGC GGTTCTCTTTCTCTGGCAGCACACTGGTCTCTAGTTCAGCAGACCCCAAAGGCCACCTTGAGACACCTAT TTGGATTGAGCGAGTAGTCATCATGGGGGCTGGAAAGCCAGCAGCTGTGGTGCTCCAGACCAAAGGATCC CCTGAAAGTCGCCTGTCCTTCCAGCATGACCCTGAGACCTCAGTGCTGATATTGCGTAAACCTGGTGTCA GCGTGGCATCCGACTGGAGTATTCATCTTCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231409 representing NM_001293621 Red=Cloning site Green=Tags(s) MAAIAAVAARRRRSWLSLVLAYLGVCLGITLAVDRSNFKTCDESSFCKRQRSIRPGLSPYRALLDTLQLG PDALTVHLIHEVTKVLLVLELQGLQKNMTRIRIDELEPRRPRYRVPDVLVADPPTARLSVSGRDDNSVEL TVAEGPYKIILTAQPFRLDLLEDRSLLLSVNARGLMAFEHQRAPRVPQESKDPAEGNGAQPEATPGDGDK PEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVYGIPEHADSLRLKVTEGGEPYRLY NLDVFQYELNNPMALYGSVPVLLAHSFHRDLGIFWLNAAETWVDISSNTAGKTLFGKMLDYLQGSGETPQ TDIRWMSESGIIDVFLMLGPSVFDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHN MPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYV KTRDGSDYEGWCWPGSASYPDFTNPRMRAWWSNMFSFDNYEGSAPNLYVWNDMNEPSVFNGPEVTMLKDA VHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGSQRFGAVWTGDNTAEWDHLKISI PMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDA LFQRYSLLPFWYTLFYQAHKEGFPVMRPLWVQYPEDMSTFSIEDQFMLGDALLIHPVSDAGAHGVQVYLP GQEEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKDDPITLFVALSPQGTA QGELFLDDGHTFNYQTRHEFLLRRFSFSGSTLVSSSADPKGHLETPIWIERVVIMGAGKPAAVVLQTKGS PESRLSFQHDPETSVLILRKPGVSVASDWSIHLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293621
ORF Size	2832 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293621.1, NP_001280550.1
RefSeq Size	3908 bp
RefSeq ORF	2835 bp
Locus ID	14376
UniProt ID	Q8BHN3
MW	107.4 kDa
Gene Summary	Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:28375157).[UniProtKB/Swiss-Prot Function]

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