Ano6 (NM_001253813) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900059G15Rik; AA407480; AW554778; F730003B03Rik; Tmem16f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231401 representing NM_001253813 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGATGATGACTAGGAAGGTCCTGCTGAACATGGAGCTGGAGGAGGACGACGATGAGGATGGAGACA TTGGTGATGTTCCTGACTCCAGGAGGCCTTTTCTGGCTCCTCATACTCCTCTACCATCCGGTCTGGTGCT GGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTTCAGGACT CCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCGAATCGACT TCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAACAGAAGAG GAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGATCTGTTTCT GATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTATGCTGAGATCATGC ACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAACTGGTTCAC CAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTTTTGAGAAG AGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGCCGCATTG TTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAATTTGGGATTAATAGACT GGTCAGCTCTGGAATCTACAAAGCAGCGTTTCCTCTGCACGACTGCAGATTCAACTATGAGTCGGAGGAC ATCAGTTGTCCTAGCGAGCGTTACCTCCTGTACAGAGAATGGGCTCACCCTCGGAGTATATACAAGAAGC AGCCCTTGGATCTTATCAGGAAGTATTACGGCGAGAAGATTGGAATCTACTTTGCTTGGCTGGGCTATTA CACGCAGATGCTCCTTCTCGCAGCTGTGGTGGGCGTGGCCTGCTTCCTCTATGGATATCTTGATCAGGAT AACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAGATCCTGATGTGCCCCCAGTGTG ACAGGCTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCTTCTAAGAAATTGTGCATCTTTGACAG TTTTGGAACCCTGATCTTCGCTGTATTTATGGGAGTGTGGGTTACATTGTTTTTGGAGTTTTGGAAGCGG CGCCAGGCAGAGCTCGAATATGAATGGGACACCGTTGAGCTACAGCAAGAGGAACAGGCCCGGCCAGAGT ATGAGGCCCAGTGCAATCACGTGGTGATAAACGAGATCACTCAGGAGGAGGAGCGCATCCCATTTACCAC CTGTGGGAAGTGCATCCGGGTCACGCTGTGTGCGAGCGCCGTCTTCTTCTGGATCCTGCTCATCATCGCC TCTGTGATCGGGATCATTGTCTACAGGCTGTCCGTGTTCATCGTATTTTCCACAACGCTCCCCAAGAACC CCAACGGGACAGACCCGATCCAGAAGTACCTGACCCCACAGATGGCCACATCCATCACAGCCTCCATCAT CAGCTTCATCATCATCATGATCCTCAACACGATCTACGAGAAGGTGGCCATCATGATCACCAACTTCGAG CTCCCAAGGACCCAGACGGATTATGAGAACAGCCTGACCATGAAGATGTTCTTGTTCCAGTTTGTCAACT ATTACTCCTCATGCTTCTACATCGCATTCTTCAAGGGCAAATTCGTGGGCTATCCCGGGGACCCAGTGTA CTTGCTGGGCAAATACAGAAGCGAAGAGTGTGACCCGGGGGGCTGCCTCCTTGAACTGACCACACAGCTG ACGATCATCATGGGGGGAAAGGCAATCTGGAACAACATACAAGAAGTCTTGCTCCCATGGGTTATGAATC TAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATTACCATCT GCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGTTCGTCACT TTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAATAAGAGTGG ACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCGCGTGGCA GCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACGCCATGATCATTGCTTTCACATCAGAC ATGATCCCCCGCCTGGTGTATTATTGGTCTTTCTCTATCCCTCCCTACGGGGACCACACTTATTACACCA TGGACGGCTACATCAACAACACTCTCTCTGTCTTCAACATCACGGACTTCAAGAACACAGACAAAGAAAA CCCATACATTGGGCTTGGTAACTATACCTTGTGCAGGTACCGTGACTTCCGAAACCCACCTGGGCACCCA CAAGAGTATAAACACAACATCTACTATTGGCACGTGATTGCCGCCAAGCTGGCCTTCATCATTGTCATGG AGCACATCATCTACTCTGTGAAGTTCTTCATTTCCTACGCAATTCCAGATGTGTCCAAAATCACGAAAAG CAAGATCAAGAGGGAGAAATACCTCACGCAGAAGTTGCTTCACGAGAGTCACCTCAAAGACCTGACCAAA AACATGGGGATCATCGCCGAGAGGATAGGAGGAACGGTTGACAACAGCGTGCGGCCAAAACTCGAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231401 representing NM_001253813 Red=Cloning site Green=Tags(s) MQMMTRKVLLNMELEEDDDEDGDIGDVPDSRRPFLAPHTPLPSGLVLENFDQTIVCPTFGSLENQQDFRT PEFEEFNGKPDSLFFTDGQRRIDFILVYEDESKKENNKKGTNEKQKRKRQAYESNLICHGLQLEATRSVS DDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEK SRMNDFYILDRDSFFNPATRSRIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCRFNYESED ISCPSERYLLYREWAHPRSIYKKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLDQD NCTWSKEVCDPDIGGQILMCPQCDRLCPFWRLNITCESSKKLCIFDSFGTLIFAVFMGVWVTLFLEFWKR RQAELEYEWDTVELQQEEQARPEYEAQCNHVVINEITQEEERIPFTTCGKCIRVTLCASAVFFWILLIIA SVIGIIVYRLSVFIVFSTTLPKNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITNFE LPRTQTDYENSLTMKMFLFQFVNYYSSCFYIAFFKGKFVGYPGDPVYLLGKYRSEECDPGGCLLELTTQL TIIMGGKAIWNNIQEVLLPWVMNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVT LFVASFPLAPLLALVNNILEIRVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSD MIPRLVYYWSFSIPPYGDHTYYTMDGYINNTLSVFNITDFKNTDKENPYIGLGNYTLCRYRDFRNPPGHP QEYKHNIYYWHVIAAKLAFIIVMEHIIYSVKFFISYAIPDVSKITKSKIKREKYLTQKLLHESHLKDLTK NMGIIAERIGGTVDNSVRPKLE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253813
ORF Size	2796 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253813.1, NP_001240742.1
RefSeq Size	5725 bp
RefSeq ORF	2799 bp
Locus ID	105722
UniProt ID	Q6P9J9
MW	108.9 kDa
Gene Summary	Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.[UniProtKB/Swiss-Prot Function]

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