Egflam (NM_001289498) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5930412K08; AU040377
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231284 representing NM_001289498 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTGTGGTTTCCGATTCTGAAGTGGCCCTTTCTTGGAGACCTGGAGAGAATGAAGGAAGTGCCCC AATTCAGTCCTACTCTGTGGAATTCATTAGATTTTGACAAGAGCTGGACCATAATCCAAGAGCGCCTGCA GATGGACTCCATGGTTATCAAAGGCCTTGATCCAGATACCAACTACCAGTTTGCTGTGAAAGCCATGAAT GCTCACGGCTTCAGTCCGCGCAGCTGGCCCAGCAACACCGTCCGAACCCTGGGTCCCGGGGAGGCTGGAA GTGGTCATTATGGACCTGGGTATATCACCAATCCCGGTGTGAGTGAAGATGATGATGGATCTGAAGACGA ACTGGACCTGGATGTATCCTTTGAGGAGGTTAAACCACTTCCTGCTACCAAAGTTGGGAATAAGAAGTTC TCAGTGGAGTCCAAGAAGACTTCTGTCTCCAACTCAGTGATGGGCTCCAGACTCGCCCAACCTACCTCAG CCTCTCTCCACGAAACTACAGTGGCCATCCCACCCACACCAGCCCAACGGAAGGGGAAGAACAGTGTGGC CATGATGTCAAGGCTCTTTGACATGTCCTGTGATGAAACTCTCTGTTCAGCTGACAGCTTCTGTGTCAAT GACTACGCCTGGGGGGGCTCACGATGCCATTGCAACCTGGGCAAAGGAGGCGAGGCCTGCTCAGAAGATA TCTTTATTCAGTATCCTCAGTTCTTTGGCCACTCCTACGTAACCTTTGAGCCTCTGAAGAACTCTTATCA GGCATTTCAGGTTACTCTGGAGTTCAGGGCAGAGGCGGAGGATGGCTTGCTGCTCTACTGCGGAGAGAGT GAGCACGGAAGAGGAGACTTCATGTCCCTGGCTCTCATCCGGCGGTCCCTCCACTTCAGGTTTAATTGTG GAACTGGGATTGCCATCATCATAAGTGAGACCAAAATCAAACTTGGGGCCTGGCATACGGTGACACTGTA CAGAGATGGCCTGAATGGGATGCTGCAGTTGAATAATGGCACCCCAGTGACAGGCCAGTCCCAGGGTCAG TACAGTAAAATTACCTTCCGGACACCTCTGTACCTCGGGGGCGCTCCCAGTGCTTACTGGCTGGTCAGAG CAACAGGGACAAACCGTGGCTTCCAAGGCTGTGTGCAGTCACTTTCTGTTAATGGGAAGAAGATTGACAT GAGGCCCTGGCCATTAGGAAAAGCCCTCAATGGAGCTGATGTTGGGGAGTGCAGCAGTGGGATCTGTGAC GAAGCCTCCTGCATCCACGGGGGCACCTGCGCAGCCATCAAGGCCGACTCCTACATCTGCCTCTGTCCCC TTGGGTTCAGAGGTCGACACTGTGAAGATGCTTTTGCCCTGACCATCCCTCAGTTCAGAGAGTCGCTGAG GTCCTATGCTGCCACACCCTGGCCCCTGGAGCCTCAGCATTATCTTTCTTTCACCGAGTTCGAGATCACC TTTCGGCCAGACTCGGGGGATGGTGTCCTTTTGTACAGCTACGACACGGGCAGCAAGGACTTCCTCTCCA TCAATATGGCTGCGGGCCACGTGGAATTCCGCTTCGACTGTGGTTCTGGGACGGGAGTTCTCAGGAGTGA GGCTCCCCTCACCCTAGGCCAATGGCATGACCTACGTGTGTCTCGCACAGCGAAGAATGGGATCTTACAG GTGGACAAGCAGAAGGTGGTAGAGGGAATGGCAGAGGGAGGCTTCACCCAGATTAAGTGTAATACGGACA TTTTTATTGGTGGAGTCCCAAATTACGATGATGTCAAGAAGAACTCGGGGATCCTCCATCCATTTAGTGG GAGCATTCAGAAGATCATCCTCAACGACCGGACCATCCATGTGAAGCATGACTTTACATCGGGCGTGAAT GTGGAGAACGCAGCCCACCCCTGTGTAGGGGCCCCCTGTGCCCATGGGGGCAGCTGTCGGCCCAGGAAGG AGGGTTATGAGTGCGACTGTCCCTTGGGCTTCGAAGGCCTGAACTGCCAGAAAGAGTGTGGGAACCACTG CCTCAATACTATTATAGAAGCCATCGAGATCCCACAGTTCATCGGCCGCAGCTACCTGACGTATGACAAC CCAAATATCCTCAAGAGGGTGTCTGGATCGAGGTCAAATGCGTTCATGAGGTTTAAGACAACCGCCAAGG ATGGCCTGTTGCTGTGGAGGGGGGACAGCCCCATGAGACCTAACAGTGACTTCATTTCCCTGGGTCTTCG GGATGGAGCCCTGATATTCAGCTACAACCTGGGCAGTGGCGTGGCTTCCATCATGGTGAACGGTTCCTTC AGCGATGGCCGGTGGCACCGAGTCAAGGCTGTCAGGGATGGCCAGTCCGGGAAGATTACAGTGGATGACT ACGGAGCCAGAACAGGCAAGTCACCGGGCTTGATGCGGCAGCTTAACATCAACGGTGCTCTGTACGTGGG TGGAATGAAGGAAATCGCTCTGCACACGAACAGACAATACCTGCGAGGCCTTGTGGGCTGCATCTCCCAT TTCACACTGTCCACCGATTACCACATTTCCCTCGTGGAAGACGCCGTGGATGGGAAGAACATCAACACTT GTGGGGCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231284 representing NM_001289498 Red=Cloning site Green=Tags(s) MSLWFPILKWPFLGDLERMKEVPQFSPTLWNSLDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVKAMN AHGFSPRSWPSNTVRTLGPGEAGSGHYGPGYITNPGVSEDDDGSEDELDLDVSFEEVKPLPATKVGNKKF SVESKKTSVSNSVMGSRLAQPTSASLHETTVAIPPTPAQRKGKNSVAMMSRLFDMSCDETLCSADSFCVN DYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAEDGLLLYCGES EHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQ YSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGADVGECSSGICD EASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQHYLSFTEFEIT FRPDSGDGVLLYSYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQ VDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVN VENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDN PNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGSF SDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQYLRGLVGCISH FTLSTDYHISLVEDAVDGKNINTCGAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289498
ORF Size	2601 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289498.1, NP_001276427.1
RefSeq Size	4883 bp
RefSeq ORF	2604 bp
Locus ID	268780
MW	95.5 kDa
Gene Summary	Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.[UniProtKB/Swiss-Prot Function]

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