Hdac7 (NM_001204281) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5830434K02Rik; HD7; HD7a; Hdac7a; mFLJ00062
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231257 representing NM_001204281 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGCGGGTGGGCCAGCGGCCCACGGTGGAGCCCCCACCAGAGCCTGCGCTGCTGACCCTGCAAC ACCCCCAACGCCTGCACCGCCATCTCTTCCTGGCAGGCTTACACCAGCAACAGCGCTCAGCCGAGCCCAT GAGGCTCTCCATGGACCCACCAATGCCGGAGCTGCAGGGGGGACAGCAGGAGCAAGAACTTCGGCAACTT CTCAATAAAGACAAGAGCAAGCGAAGTGCCGTAGCCAGCAGTGTGGTCAAGCAGAAGCTGGCTGAAGTGA TCCTGAAGAAACAGCAGGCAGCCCTTGAGAGAACAGTCCATCCCAGCAGCCCCAGTATTCCCTACAGAAC TCTTGAGCCCTTGGACACAGAGGGTGCTGCCCGCTCCGTGCTTAGCAGCTTCCTGCCTCCTGTTCCCAGC CTGCCCACTGAACCCCCGGAACACTTTCCCTTGCGTAAAACAGTGTCTGAACCCAACCTGAAGTTGCGCT ACAAACCCAAGAAATCCCTGGAGAGACGCAAGAATCCCCTGCTCAGGAAGGAGAGTGCCCCGCCCAGCCT TCGGAGGAGGCCTGCCGAGACCCTTGGAGATTCCTCCCCCAGTAGTAGCAGCACACCCGCGTCAGGGTGC AGCTCCCCTAATGACAGCGAGCATGGCCCTAACCCTGCCCTAGGCTCAGAGGCTGATGGTGACCGCAGGA CCCATTCAACTTTAGGCCCTCGGGGTCCTGTACTGGGGAACCCCCATGCTCCCCTCTTCCTGCACCACGG TCTGGAGCCAGAGGCTGGGGGCACCTTACCCTCTCGCCTGCAACCCATTCTCCTGCTGGACCCCTCAGTC TCTCATGCCCCACTGTGGACTGTGCCTGGCCTTGGGCCCTTGCCCTTCCACTTTGCCCAGCCCTTACTGA CCACCGAGCGGCTCTCTGGGTCAGGCCTCCATCGACCACTTAACCGGACCCGCTCAGAGCCCCTGCCCCC CAGCGCCACAGCCTCCCCTCTGCTGGCCCCCCTGCAGCCCCGCCAGGATCGGCTCAAACCTCACGTCCAG CTGATCAAGGTGCCACCCTGGGAGCAGCAGCATCTAGCCGGGCGGCTCTCTCAGGGAAGCCCCGGGGACT CCGTGCTGATACCTCTGGCCCAGGTTGGACACCGGCCCCTGTCCAGAACCCAGTCTTCCCCAGCAGCACC TGTCTCCCTGCTGAGCCCAGAGCCCACCTGTCAGACCCAAGTCCTCAACAGCTCAGAGACACCTGCTACA GGGCTGGTCTATGACTCGGTGATGCTGAAACACCAATGTTCCTGTGGAGACAACAGCAAGCATCCCGAGC ATGCAGGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAACGGGGTCTCCGCAGCCAGTGTGAGTGTCT CCGAGGCCGAAAGGCTTCCCTAGAGGAGCTGCAGTCAGTCCACTCTGAACGGCACGTGCTCCTCTACGGC ACGAACCCACTCAGCCGCCTCAAACTGGATAACGGGAAGCTTACAGGACTCCTGGCACAGCGGACGTTTG TGATGCTACCCTGTGGCGGGGTTGGGGTCGATACTGACACCATCTGGAACGAGCTGCATTCCTCCAATGC AGCCCGCTGGGCTGCGGGCAGTGTCACCGACCTTGCCTTCAAAGTAGCTTCCCGAGAGCTGAAGAATGGC TTTGCTGTGGTGCGACCCCCGGGACACCATGCAGATCATTCTACAGCCATGGGCTTCTGCTTCTTCAACT CCGTGGCCATCGCCTGCCGACAGCTACAGCAACACGGCAAAGCCAGCAAGATCCTCATTGTTGACTGGGA TGTTCACCATGGCAACGGCACACAGCAGACTTTCTACCAGGACCCCAGTGTGCTCTACATTTCCCTTCAT CGCCATGACGACGGCAACTTCTTCCCAGGCAGTGGGGCCGTGGATGAGGTGGGAACTGGCAGTGGCGAGG GCTTCAATGTCAACGTGGCTTGGGCTGGGGGCTTGGATCCACCCATGGGGGATCCTGAGTACCTGGCTGC TTTCAGGATAGTGGTGATGCCCATTGCCCGAGAGTTTGCTCCAGACCTGGTCCTGGTGTCTGCTGGGTTT GATGCTGCGGAGGGTCACCCAGCCCCGCTGGGTGGCTACCATGTTTCTGCCAAATGTTTTGGGTACATGA CGCAGCAGTTGATGAACTTGGCAGGAGGCGCCGTGGTGTTGGCCTTAGAGGGTGGACATGACCTCACGGC CATCTGTGATGCCTCGGAGGCCTGTGTAGCTGCTCTTCTGGGCAACAAGGTGGACCCCCTTTCAGAAGAA AGCTGGAAACAGAAACCCAACCTCAGTGCCATCCGCTCGCTGGAAGCTGTGGTCAGGGTGCACAGGAAAT ACTGGGGCTGCATGCAGCGCTTGGCCTCCTGTCCAGACTCCTGGCTACCCAGAGTGCCGGGAGCTGATGC AGAAGTGGAAGCCGTGACCGCGCTGGCATCCCTTTCTGTGGGCATCCTGGCTGAAGACAGGCCCTCGGAG CGGCTGGTGGAAGAGGAAGAACCCATGAACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231257 representing NM_001204281 Red=Cloning site Green=Tags(s) MDLRVGQRPTVEPPPEPALLTLQHPQRLHRHLFLAGLHQQQRSAEPMRLSMDPPMPELQGGQQEQELRQL LNKDKSKRSAVASSVVKQKLAEVILKKQQAALERTVHPSSPSIPYRTLEPLDTEGAARSVLSSFLPPVPS LPTEPPEHFPLRKTVSEPNLKLRYKPKKSLERRKNPLLRKESAPPSLRRRPAETLGDSSPSSSSTPASGC SSPNDSEHGPNPALGSEADGDRRTHSTLGPRGPVLGNPHAPLFLHHGLEPEAGGTLPSRLQPILLLDPSV SHAPLWTVPGLGPLPFHFAQPLLTTERLSGSGLHRPLNRTRSEPLPPSATASPLLAPLQPRQDRLKPHVQ LIKVPPWEQQHLAGRLSQGSPGDSVLIPLAQVGHRPLSRTQSSPAAPVSLLSPEPTCQTQVLNSSETPAT GLVYDSVMLKHQCSCGDNSKHPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYG TNPLSRLKLDNGKLTGLLAQRTFVMLPCGGVGVDTDTIWNELHSSNAARWAAGSVTDLAFKVASRELKNG FAVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQHGKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLH RHDDGNFFPGSGAVDEVGTGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFAPDLVLVSAGF DAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNKVDPLSEE SWKQKPNLSAIRSLEAVVRVHRKYWGCMQRLASCPDSWLPRVPGADAEVEAVTALASLSVGILAEDRPSE RLVEEEEPMNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204281
ORF Size	2553 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204281.1, NP_001191210.1
RefSeq Size	3791 bp
RefSeq ORF	2556 bp
Locus ID	56233
MW	92.7 kDa
Gene Summary	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Positively regulates the transcriptional repressor activity of FOXP3 (By similarity). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (By similarity). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function]

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