Otud7b (NM_001025614) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; Trabid2; Za20d1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231241 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGGACATGGATGCTGTCCTGTCGGATTTTGTTCGTTCCACAGGTGCAGAGCCAGGGCTGGCTC GAGATCTTCTAGAAGGAAAGAACTGGGATGTGAGTGCTGCCCTCAGTGATTTTGAACAGTTGCGTCAAGT CCATGCTGGGAACCTATCCCCACCCTTTAGTGGAGGGAGTACTTGCCCTAAGACCCCTGAAAAAGGGGGT TCGGATAGAGAGCCCACTCGCCCTTCTCGACCCATCCTTCAGAGACAAGATGACGTCATTCAAGAAAAAC GCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGCTCCCATGTCTCCTCCAA TGGTGGGGGTGGGGGGAGCAGTGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTC ACTGTGTACAAAGAAGACTTCCGCAGCTTCATAGAGCGAGACCTCATTGAGCAGTCCATGCTGGTTGCCT TGGAACAGGCAGGGCGTCTGAATTGGTGGGTTAGTATGGACTCCACCTGCCAGAGGCTGCTCCCTTTAGC AACTACTGGGGATGGGAACTGCCTCCTGCATGCAGCATCTCTTGGGATGTGGGGTTTCCATGATAGAGAC TTGGTGCTGAGGAAAGCGCTGTATGCACTGATGGAGAAGGGGGTGGAGAAGGAAGCACTGAGAAGGCGCT GGAGGTGGCAGCAAACACAGCAGAATAAAGAGTCAGGACTGGTGTATACGGAAGATGAGTGGCAGAAGGA ATGGAATGAATTGATCAAACTTGCTTCAAGTGAACCCAGAATGCATCTGGGTTCCAACGGAGCCAGTGGT GGTGGAGTGGAGAGCTCCGAGGAGCCGGTATATGAAAGCCTAGAGGAATTCCATGTCTTCGTCCTTGCTC ATGTGCTCAAGAGGCCCATAGTGGTCGTGGCAGACACCATGCTGAGGGACTCTGGAGGGGAAGCATTTGC CCCTATCCCATTTGGAGGGATCTATCTGCCATTGGAGGTTCCAGCCAGCCAGTGTCACCGATCCCCTCTG GTGCTCGCCTATGATCAGGCCCACTTCTCTGCGCTTGTGTCGATGGAGCAGAAGGAGAGCGCCAAAGAAC AAGCTGTGATCCCACTCACAGATTCAGAGCACAAGCTGCTGCCCCTGCACTTTGCTGTGGACCCAGGAAA GGGCTGGGAGTGGGGCAAAGATGACAATGACAATGTCCGATTGGCCAGTATAATTCTGTCTCTGGAGGTG AAGTTACATCTGCTGCATAGCTATATGAATGTGAAGTGGATCCCACTGTCTTCTGATTCACAAGCTCCCC TAGCTCAGCCTGAGTCCCCCACAGCCTCAGCTGGAGATGAACCCAGGTCCACTCCTGAGTCTGGGGAGTC AGACAAGGAGTCGGTTGGCAGCAGTTCTCTCGGCAACGAGGGCAGCAGGCGGAAGGAGAAGTCAAAGCGA GACCGGGAGAAGGACAAGAAGAGAGCAGATTCCGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGG GCAGCAAGCTTAAGAAGAACATGGGGGGCCTGATGCACAGCAAGGGCCCCAAGCCTGGAGGGCTGGGGAG TGGGTCTGGAATAAGCAGTGGTACGGAGACATTAGAAAAGAAGAAGAAGAACAACACATTGAAGAGCTGG AAGGGTGGCAAGGAAGAGGCTGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCGTCTGAGTCTGTTGGCA ATGGCGGGAGCAAGTATAGCCAGGAGGTGATGCAAAGCCTGAGCACTATGAGGATCGCAATGCAAGGGGA GGGCAAGTACATTTTTGTTGGAACCCTGAAGATGGGCCACCGGCACCAGTATCAGGAGGAGATGATCCAG CGTTACCTTGCAGATGCTGAGGAAAGATTCCTGGCAGAGCAGAAGCAGAAGGAGGTTGAGAGGAAGATCA TGAATGGAGGGTTAGTTAGTGGGCCTCCTCCAGCCAAAAAGCCAGAGCCGGACGGTGGGGAGGACCAGCC CAGTGACTCCCCCGCAGAGCCCAAGGCGATGGCCTTCTCTACTGCTTACCCTGGGGGCTTTACTATCCCT CGGCCTTCTGGGGGTGGAGTCCACTGCCAGGAACCCCGGAGGCAATTGGCAGGGGGGCCATGTGTAGGGG GCTTACCATCATATGCCACCTTTCCCAGACAGTATCCTGGGCGACCGTACCCCCACCAGGACAACATCCC AGCTCTGGAGCCAGGCAAAGATGGAGTTCACAGGGGTGCCTTGTTACCACCCCAGTTCCGTGTGGCTGAT TCCTATAGCAATGGCTACAGAGAGCCCCCAGAGCCAGATGGATGGGCTGGAGCTCCACGGGGACTTCCCC CAACCCAGACCAAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAACTTGTGCTC TTGCTGTTACAGGGAAGAACTGAGGCGGAGGGAGCGGGAACCTGGTGGGGAACTGCTGGCACACAGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231241 protein sequence Red=Cloning site Green=Tags(s) MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVSAALSDFEQLRQVHAGNLSPPFSGGSTCPKTPEKGG SDREPTRPSRPILQRQDDVIQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSSEHPLEMPICAFQLPDL TVYKEDFRSFIERDLIEQSMLVALEQAGRLNWWVSMDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRD LVLRKALYALMEKGVEKEALRRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSNGASG GGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPL VLAYDQAHFSALVSMEQKESAKEQAVIPLTDSEHKLLPLHFAVDPGKGWEWGKDDNDNVRLASIILSLEV KLHLLHSYMNVKWIPLSSDSQAPLAQPESPTASAGDEPRSTPESGESDKESVGSSSLGNEGSRRKEKSKR DREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGPKPGGLGSGSGISSGTETLEKKKKNNTLKSW KGGKEEAAGDGPVSEKPPSESVGNGGSKYSQEVMQSLSTMRIAMQGEGKYIFVGTLKMGHRHQYQEEMIQ RYLADAEERFLAEQKQKEVERKIMNGGLVSGPPPAKKPEPDGGEDQPSDSPAEPKAMAFSTAYPGGFTIP RPSGGGVHCQEPRRQLAGGPCVGGLPSYATFPRQYPGRPYPHQDNIPALEPGKDGVHRGALLPPQFRVAD SYSNGYREPPEPDGWAGAPRGLPPTQTKCKQPNCSFYGHPETNNLCSCCYREELRRREREPGGELLAHRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001025614
ORF Size	2523 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001025614.1, NP_001020785.1
RefSeq Size	8043 bp
RefSeq ORF	2523 bp
Locus ID	229603
UniProt ID	B2RUR8
MW	92 kDa
Gene Summary	Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B (PubMed:23334419). Negatively regulates mucosal immunity against infections (PubMed:23334419). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (PubMed:26903241). Mediates deubiquitination of EGFR (By similarity). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin (By similarity).[UniProtKB/Swiss-Prot Function]

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