Smarcad1 (NM_001253392) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AV081750; AW226546; D6Pas1; Etl1; mKIAA1122
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231231 representing NM_001253392 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGAGCCATTGCTGCTGCCTTGCTGATGTTTGGTGATGCAGGTGGTGGACCCAGGAAAAGGAAAT TATCTTCTTCTTCTGAGGAGGATGACGTTAATGATGATCAGTCTGTAAAACAGCCGCGAGGGGACCGTGG AGAAGAATCAAATGAGTCTGCAGAAGCCAGCAGCAACTGGGAAAAGCAAGAAAGTATTGTGTTGAAATTG CAAAAGGAATTTCCAAATTTTGATAAACAGGAATTAAGAGAAGTGCTTAAGGAGCATGAGTGGATGTACA CAGAAGCCTTGGAGTCTCTGAAAGTGTTTGCGGAAGATCAAGACGTGCAGTGTGCTTCACAAAGTGAAGT TACAAATGGAAAAGAAGTTGCAAGGAATCAAAATTATTCTAAAAATGCAACTAAAATAAAAATGAAACAG AAAATTTCCGTGAAACCACAAAATGGCTTTAACAAGAAACGTAAGAAAAATGTATTTAATCCTAAGAAAG CCGTAGAGGACTCTGAGTACGATTCAGGTTCTGATGCTGGGAGCTCACTGGATGAGGACTATAGCAGTTG TGAAGAAGTGATGGAGGACGGCTATAAAGGCAAAATTCTTCATTTCCTTCAAGTTTCTTCAATTGCTGAA CTGACTTTGATTCCTAAGTGTTCTCAGAAAAAGGCTCAGAAAATAACAGAACTCCGGCCCTTTAATAATT GGGAAGCTCTGTTTACAAAGATGTCTAAAATCAATGGCCTATCTGAAGATTTGATATGGAATTGCAAAAC GGTAATTCAAGAAAGGGACGTAGTTATAAGACTTATGAACAAATGTGAGGACATTTCAAATAAACTGACC AAACAAGTTACGATGCTCACCGGGAATGGAGGCGGATGGAACAGAGAACAGCCCTCCCTTCTAAACCAAA GTTTGTCACTCAAGCCCTATCAGAAGGTTGGGTTGAACTGGCTGGCGTTGGTGCATAAGCATGGACTTAA TGGCATTCTGGCAGACGAAATGGGCCTAGGAAAAACCATTCAAGCCATTGCATTCCTGGCATACCTCTTT CAAGAGGGTAATAAAGGGCCTCATTTGATTGTTGTTCCAGCCTCCACCATAGATAACTGGTTAAGAGAAG TTAACTTATGGTGCCCCAGTTTAAATGTGCTCTGTTACTATGGTTCTCAAGAAGAGCGTAAGCAAATTAG ATTCAACATCCATAATAAGTATGAGGATTACAATGTAATTGTTACAACGTATAATTGTGCAATCAGCAGC TCTGATGACCGCAGTCTCTTTCGACGACTGAAACTGAATTATGCAATTTTTGATGAAGGCCATATGCTGA AGAATATGGGCTCTATCCGCTATCAACATCTAATGACAATTAATGCACGTAACCGTTTATTGCTCACAGG CACACCTGTGCAGAATAATCTGCTAGAACTCATGTCATTGTTGAATTTTGTTATGCCACACATGTTTAGC AGTAGCACCAGTGAAATCCGAAGGATGTTTTCTTCTAAAACGAAACCAGCAGACGAGCAGAGTATATACG AAAAGGAGAGAATAGCCCACGCGAAGCAAATCATTAAGCCCTTCATCCTCAGGAGAGTGAAGGAGGAGGT TCTTAAGCTCCTGCCTCCCAAGAAGGATCGGATTGAGCTATGTGCGATGTCAGAGAAGCAGGAGCAGCTC TACTCGGGCCTCTTCAACAGATTGAAAAAGTCTATCAACAACCTGGAAAAAAACACAGAGATGTGCAATG TCATGATGCAATTGAGAAAAATGGCCAATCACCCTTTACTACACCGCCAGTATTACACACCTGAGAAACT GAAGGAGATGTCTCAGCTAATGCTGAAGGAACCTACGCACTGTGAGGCCAACCCTGACCTGATCTTTGAA GACATGGAAGTTATGACAGATTTTGAACTACATGTACTTTGTAAACAGTATCAACACATTAATAGTTACC AGTTAGACATGGATTTAATTTTAGATTCTGGGAAATTCCGAGCCTTAGGATGCATCTTGTCTGAGTTGAA ACAGAAGGGTGATAGAGTTGTATTATTCAGCCAGTTTACCATGATGCTGGATATACTAGAGGTTCTCTTA AAGCATCATCAACATAGGTACCTCCGATTAGATGGAAAGACTCAGATTTCTGAAAGGATTCATCTAATTG ATGAGTTTAATACAGATATGGATATCTTTGTATTTCTCTTGTCAACTAAAGCTGGTGGACTAGGAATAAA TCTTACTTCAGCAAATGTTGTTATACTTCACGACATTGATTGCAATCCATACAATGACAAACAAGCAGAA GACAGGTGCCATAGAGTTGGTCAGACTAAAGAAGTATTAGTTATTAAATTAATAAGCCAAGGAACTATTG AAGAGTCCATGCTAAAAATTAACCAACAAAAGTTGAAACTAGAGCAAGACATGACCACAGTAGATGAAGC TGATGAAGGAAGTATGCCAGCAGATATAGCCACATTACTAAAAACATCAATGGGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231231 representing NM_001253392 Red=Cloning site Green=Tags(s) MDGAIAAALLMFGDAGGGPRKRKLSSSSEEDDVNDDQSVKQPRGDRGEESNESAEASSNWEKQESIVLKL QKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDVQCASQSEVTNGKEVARNQNYSKNATKIKMKQ KISVKPQNGFNKKRKKNVFNPKKAVEDSEYDSGSDAGSSLDEDYSSCEEVMEDGYKGKILHFLQVSSIAE LTLIPKCSQKKAQKITELRPFNNWEALFTKMSKINGLSEDLIWNCKTVIQERDVVIRLMNKCEDISNKLT KQVTMLTGNGGGWNREQPSLLNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLF QEGNKGPHLIVVPASTIDNWLREVNLWCPSLNVLCYYGSQEERKQIRFNIHNKYEDYNVIVTTYNCAISS SDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINARNRLLLTGTPVQNNLLELMSLLNFVMPHMFS SSTSEIRRMFSSKTKPADEQSIYEKERIAHAKQIIKPFILRRVKEEVLKLLPPKKDRIELCAMSEKQEQL YSGLFNRLKKSINNLEKNTEMCNVMMQLRKMANHPLLHRQYYTPEKLKEMSQLMLKEPTHCEANPDLIFE DMEVMTDFELHVLCKQYQHINSYQLDMDLILDSGKFRALGCILSELKQKGDRVVLFSQFTMMLDILEVLL KHHQHRYLRLDGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAE DRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEADEGSMPADIATLLKTSMGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253392
ORF Size	2508 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253392.1, NP_001240321.1
RefSeq Size	4637 bp
RefSeq ORF	2511 bp
Locus ID	13990
UniProt ID	Q04692
MW	95.8 kDa
Gene Summary	DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).[UniProtKB/Swiss-Prot Function]

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