Rabep1 (NM_001291141) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rab5ep; rabaptin-5; rabaptin-5alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231209 representing NM_001291141 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCCGGGCCCGGCTCCCCAGCCTGACGAGGATCTGAAGAGGCAAAATGCAGTATTGCAAGCTG CACAGGACGATTTGGGTCACCTTCGGACACAGCTGTGGGAGGCTCAGGCAGAGATGGAGAACATTAAGGC AATTGCCACAGTCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAAGGCAATGGAGAGAAGAA GTTGCTTCACTTCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCAGTTTCACCTGAGGCTGG AGCAGGAGCGAGCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATAGCTGACTTAAGAAGAAG GTTGTCTGAAGGTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCCAAGAGGATGCTGAAAAG CTTCGTTCTGTCGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAAACTGACAGAAGCTGAAG ACAAGATTAAAGAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTGGAGGCCGAGAAGTCTTG CAGGACTGACCTGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGCTACAGGAAGATGCTGAG AAATTGAGGAAAGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACAACAACACAACCAGTTAA AGCATACATGGCAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTGATGAGAGACATGCAGAG AATGGAGATTGTGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGAAGAAAGACCAGGAGGAG GATGAACAACAAAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGAAGAAGAAGTAAAAATAC CAGTAGTGTGTGCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAAGAGGAGCATTTAGACAG CACCCATGGGTCAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAGATCCATTTAGTAAATCG GACAATGACATGTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTTGGGAACCTCAAGCTCAT TGCAATCCAAAGCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTGGATGAGTCGGATTTTGG ACCACTGGTTGGAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTGGGTCACTGCAGATGCCA AGTGGATTTATGTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACCTGAGCAGGAGGAGACAG CATCCCTCCTCTCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCCAGTGGTTACCGCTTAGT GAGTGAGACGGAATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATAAGCTTGGTAGACGTTGT GATATGTGTTCCAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGCTGAAACAAGAGACCAGG TGAAAAAACTGCAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACGATGAAAGAGAAGCAGGA GCTGGAGGACTTCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTGCACTTGTTTTAAGAGCC CAAGCCTCTGAGGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAAGAGGGACGTTCAGGAGC AGATGGCGGTGCTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGGTTACAGAAAGACAACGA CAGCCTCCAGGGGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACTTCATTCTTCCAGACACT GTGGAGGTGCTCCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGTGCGCACAGCAGCAGACC ACATGGAAGAGAAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAAGCGGAACAGTGTTTAAA AGAAAACCTGGAAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAATAGCTTCCATTTCTAGT CTAAAAGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTACATTAAGAGAGAAGTCGC AACAACTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAGAAAGAAACTGCTGCTAA GGCTACTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGCAGACAGAATTAGATGTC AGTGAACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCAGTTGGAGAGGATCCGGC AAGCAGACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACAGACATTAACCAGCTCCC TGAGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231209 representing NM_001291141 Red=Cloning site Green=Tags(s) MAQPGPAPQPDEDLKRQNAVLQAAQDDLGHLRTQLWEAQAEMENIKAIATVSENTKQEAIDEVKRQWREE VASLQAIMKETVRDYEHQFHLRLEQERAQWAQYRESAEREIADLRRRLSEGQEEENLENEMKKAQEDAEK LRSVVMPMEKEIAALKDKLTEAEDKIKELEASKVKELNHYLEAEKSCRTDLEMYVAVLNTQKSVLQEDAE KLRKELHEVCHLLEQERQQHNQLKHTWQKANDQFLESQRLLMRDMQRMEIVLTSEQLRQVEELKKKDQEE DEQQRVNKRKDNKKTDTEEEVKIPVVCALTQEESSTPLSNEEEHLDSTHGSVHSLDADLMLPSGDPFSKS DNDMFKDGLRRAQSTDSLGTSSSLQSKALGYNYKAKSAGNLDESDFGPLVGADSVSENFDTVSLGSLQMP SGFMLTKDQERAIKAMTPEQEETASLLSSVTQGMESAYVSPSGYRLVSETEWNLLQKEVHNAGNKLGRRC DMCSNYEKQLQGIQIQEAETRDQVKKLQLMLRQANDQLEKTMKEKQELEDFLKQSAEDSSHQISALVLRA QASEVLLEELQQSFSQAKRDVQEQMAVLMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQLAEDFILPDT VEVLRELVLKYRENIVHVRTAADHMEEKLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIASISS LKAELERIKVEKGQLESTLREKSQQLESLQEMKVNLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDV SEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLTDINQLPET TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291141
ORF Size	2457 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291141.1, NP_001278070.1
RefSeq Size	5311 bp
RefSeq ORF	2460 bp
Locus ID	54189
UniProt ID	O35551
MW	94.8 kDa
Gene Summary	Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function]

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