Dlg3 (NM_001290402) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dlgh3; mKIAA1232; SAP102
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231201 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAAGCACCAGCACTGCTGTAAGTGCCCCGAGTGCTATGAGGTGACCCGCCTGGCCGCCCTGCGGC GTCTTGAGCCTCCTGGCTACGGGGACTGGCAGGTGCCGGACCCCTATGGGCCAAGTGGGGGCAATGGGGC TAGCTCCGGTTATGGAGGCTACAGCTCGCAGACCCTGCCTTCACAGGCAGGGGCTACCCCCACCCCTCGC ACCAAGGCCAAGCTCATCCCCACAGGCCGAGATGTGGGGCCAGTGCCCCCTAAGCCAGTCCCCGGCAAGA GCACCCCAAAACTCAACGGCAGTGGCCCCGGCTGGTGGCCCGAGTGCACCTGTACCAACCGGGACTGGTA TGAGCAGGTGAATGGCAGTGATGGCATGTTCAAGTATGAGGAGATAGTACTGGAACGGGGCAACTCTGGC CTTGGCTTCAGTATCGCAGGTGGCATCGACAACCCTCATGTCCCTGATGACCCTGGCATCTTTATTACCA AGATCATTCCTGGTGGAGCAGCTGCCATGGATGGGAGGCTGGGGGTGAATGACTGTGTGCTACGGGTGAA TGAGGTGGACGTGTCCGAGGTGGTGCACAGCCGGGCCGTGGAGGCACTGAAGGAGGCAGGCCCTGTGGTG CGGTTGGTGGTGCGGAGGCGACAGCCCCCACCTGAGACTATCATGGAGGTCAACCTGCTCAAAGGGCCAA AAGGCCTAGGTTTCAGCATTGCTGGGGGCATTGGCAACCAGCACATCCCAGGAGACAACAGCATCTATAT CACCAAGATCATTGAAGGAGGAGCTGCTCAGAAGGATGGACGCCTACAGATTGGGGACCGGCTGCTTGCG GTGAACAATACCAATCTGCAGGATGTGAGGCATGAGGAAGCTGTGGCCTCACTCAAGAACACATCAGACA TGGTCTATCTGAAGGTGGCCAAGCCAGGCAGCATCCACCTCAACGATATGTATGCTCCCCCTGACTATGC CAGCACTTTCACTGCCTTGGCTGACAATCACATAAGCCATAATTCCAGCCTGGGTTATCTTGGGGCAGTG GAGAGCAAGGTCACCTACCCTGCTCCACCTCAGGTGCCCCCTACTCGTTACTCTCCTATTCCCAGACACA TGCTGGCTGAGGAAGACTTTACCAGAGAGCCCCGCAAGATCATCCTGCACAAAGGTTCTACAGGCCTGGG CTTCAACATTGTAGGAGGAGAAGATGGAGAAGGCATTTTTGTTTCCTTCATCCTGGCGGGAGGCCCAGCA GACCTAAGTGGGGAGTTGCGAAGGGGAGACCGGATCTTATCGGTAAATGGAGTCAACCTGAGGAATGCTA CCCACGAGCAAGCAGCGGCTGCTCTGAAACGGGCTGGCCAGTCAGTCACCATTGTGGCCCAGTATAGACC TGAAGAGTACAGTCGCTTTGAATCCAAGATCCATGACTTGCGGGAACAAATGATGAACAGCAGCATGAGT TCTGGGTCTGGGTCTCTCCGAACCAGTGAGAAGAGGTCCTTGTATGTCAGGGCCCTGTTTGATTATGATC GGACTCGTGACAGCTGTCTACCAAGCCAGGGACTCAGTTTCTCTTATGGTGACATTCTGCACGTCATTAA CGCCTCTGATGATGAGTGGTGGCAAGCAAGGCTGGTGACTCCTCATGGAGAGAGTGAACAGATCGGTGTG ATCCCTAGTAAAAAGAGGGTGGAAAAGAAAGAGCGAGCTCGATTGAAAACTGTGAAGTTCCATGCCAGGA CAGGGATGATTGAGTCTAATCGGGACTTCCCTGGGTTAAGTGACGATTATTATGGAGCAAAGAACCTGAA AGGACAAGAGGATGCTATTTTGTCATATGAGCCAGTGACACGACAAGAAATTCACTATGCCAGGCCTGTG ATCATCTTGGGCCCAATGAAGGACCGAGTCAACGATGACCTCATCTCTGAGTTCCCGCATAAATTTGGAT CCTGTGTGCCACATACCACCCGGCCTCGGCGTGATAACGAGGTAGATGGACAGGATTACCACTTTGTGGT TTCCCGGGAACAAATGGAGAAGGATATTCAGGACAACAAGTTCATTGAGGCGGGCCAGTTCAATGATAAT CTCTATGGGACCAGCATCCAGTCTGTGCGGGCAGTTGCAGAGAGGGGCAAGCACTGCATCTTAGATGTCT CCGGCAACGCCATAAAGAGACTGCAGCAAGCACAACTTTATCCCATTGCCATTTTCATCAAGCCCAAGTC CATTGAAGCACTTATGGAAATGAACCGACGGCAGACATACGAACAAGCAAATAAGATCTTTGATAAAGCC ATGAAACTGGAGCAAGAATTTGGAGAATACTTTACAGCCATTGTACAGGGTGACTCACTGGAAGAGATTT ATAACAAAATCAAACAAATCATTGAGGACCAGTCTGGGCACTACATTTGGGTCCCATCCCCTGAAAAACT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231201 protein sequence Red=Cloning site Green=Tags(s) MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPR TKAKLIPTGRDVGPVPPKPVPGKSTPKLNGSGPGWWPECTCTNRDWYEQVNGSDGMFKYEEIVLERGNSG LGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVV RLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLA VNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSIHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAV ESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPA DLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMS SGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGV IPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGQEDAILSYEPVTRQEIHYARPV IILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDN LYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKA MKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290402
ORF Size	2454 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290402.1, NP_001277331.1
RefSeq Size	4905 bp
RefSeq ORF	2454 bp
Locus ID	53310
MW	90.3 kDa
Gene Summary	Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.[UniProtKB/Swiss-Prot Function]

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