Dpp6 (NM_001198886) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B930011P16Rik; D5Buc3; D5Buc4; D5Buc5; Dpp-6; DPP VI; Gm1377; In(5)6H-p; Peplb; Rw
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231177 representing NM_001198886 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAAAGGCCATGATCAAGACCGCTAAAATGCAGGGCAACGTGATGGAGCTGGTGGGGAGTAATC CTCCACAGAGGAACTGGAAAGGAATCGCCATTGCGCTGCTCGTCATCCTTGTCATCTGCTCTCTGATTGT CACCTCAGTCATCCTGTTGACCCCAGCGGAAGATACCAGTCTGTCTCAGAAGAAAAAAGTGACTGTGGAA GATCTCTTCAGTGAAGACTTCAAAATCCATGACCCAGAGGCAAAGTGGATAAGCAATAAGGAATTCATCT ACAGAGAACGGAAAGGGAGTGTCATACTGCGGAATGTTGAAACAAATAATTCCACGGTGTTAATAGAAGG CAAAAAAATTGAATCCTTGAGAGCCATCAGATATGAAATATCCCCAGATAAAGAGTATGTGCTGTTCTCC TATAATGTGGAGCCGGTATACCAACACTCCCACACTGGATATTATGTTCTGAGCAAAATTCCTCATGGGG ACCCTCAGAGTCTGGACCCACCAGAAGTCAGCAACGCAAAGCTTCAGTATGCAGGGTGGGGCCCAAAAGG CCAGCAGCTGATATTTATCTTTGAGAACAACATCTACTACTGTGCACACGTTGGAAAGCAGGCAATTCGA GTGGTCTCTACAGGGAAGGAGGGCGTGATCTACAATGGCCTCAGTGACTGGCTGTATGAAGAGGAGATTC TGAAGAGCCACATCGCTCATTGGTGGTCACCAGATGGTACAAGGCTCGCCTATGCCACCATCAACGACTC CCGGGTTCCACTTATGGAGCTGCCAACGTACACAGGCTCTGTCTACCCCACTGTGAAGCCCTACCACTAT CCTAAGGCTGGCAGTGAAAACCCCAGCATCTCGCTCCATGTCATCGGCTTGAATGGACCCACCCACGACC TGGAGATGATGCCACCTGATGACCCGCGCATGAGGGAATATTACATCACCATGGTGAAATGGGCCACCAG CACCAAGGTCGCTGTGACCTGGCTGAACCGGGCCCAGAATGTGTCAATCCTGACCCTCTGTGATGCCACC ACGGGTGTCTGCACTAAGAAACATGAAGATGAAAGCGAAGCCTGGCTCCACAGACAGAATGAAGAGCCTG TGTTCTCCAAAGATGGCAGGAAGTTTTTCTTTGTCAGAGCGATCCCACAGGGAGGCCGGGGTAAATTCTA CCATATCACCGTATCATCATCCCAGCCCAACAGCAGCAATGACAACATCCAGTCTATCACCTCTGGAGAC TGGGATGTGACCAAGATCCTGTCCTATGATGAGAAGCGGAATAAGATCTATTTCCTGAGCACAGAAGACC TGCCACGGAGACGACATCTCTACAGTGCCAACACGGTAGATGACTTCAACAGGCAGTGTCTGTCATGTGA CTTGGTGGAGAACTGCACCTATGTCAGCGCTTCTTTCAGCCATAACATGGACTTCTTCCTGCTCAAGTGT GAAGGCCCTGGCGTACCCACTGTTACTGTGCATAACACCACGGACAAGAGAAGAATGTTTGACTTGGAAG CAAATGAGGAAGTTCAGAAGGCTATCAACGACCGGCAGATGCCGAAGATTGAATATCGAAAAATTGAAGT AGAAGATTACAGCTTGCCAATGCAAATCTTGAAGCCGGCCACCTTCACCGACACAGCTCACTACCCCTTA TTGCTCGTGGTGGATGGTACCCCAGGGAGTCAGAGCGTGACAGAGAGGTTTGAGGTGACCTGGGAAACAG TGTTGGTGAGCAGCCATGGAGCCGTGGTGGTCAAGTGTGACGGCCGAGGCAGCGGCTTCCAGGGAACCAA GCTCCTACAGGAAGTACGGAGGCGGCTAGGCTTCCTGGAGGAGAAGGACCAGATGGAAGCTGTAAGAACT ATGCTGAAGGAGCAGTATATTGACAAGACTCGGGTAGCTGTGTTTGGGAAGGATTATGGTGGGTACCTGA GCACTTACATCCTCCCAGCCAAGGGAGAAAATCAAGGTCAGACTTTCACCTGCGGCTCTGCGCTCTCTCC AATAACAGACTTCAAACTCTATGCCTCTGCATTTTCTGAGAGGTACCTTGGCCTCCATGGACTCGACAAC AGAGCGTATGAGATGACCAAGCTGGCACACCGAGTGTCAGCACTGGAGGACCAGCAGTTCCTGATCATCC ACGCCACGGCCGATGAAAAAATCCATTTCCAGCACACAGCTGAGCTCATCACGCAGCTGATCAAGGGAAA GGCCAATTACAGCTTACAGATCTACCCAGATGAGAGTCACTACTTCCACAGCGTGGCGCTCAAGCAGCAC CTGTCCAGGTCCATCATCGGCTTCTTTGTGGAATGTTTCAGAGTCCAAGATAAGCTACCAACAGCCACAG CAAAAGAGGAGGAGGAGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231177 representing NM_001198886 Red=Cloning site Green=Tags(s) MKEKAMIKTAKMQGNVMELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDTSLSQKKKVTVE DLFSEDFKIHDPEAKWISNKEFIYRERKGSVILRNVETNNSTVLIEGKKIESLRAIRYEISPDKEYVLFS YNVEPVYQHSHTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQAIR VVSTGKEGVIYNGLSDWLYEEEILKSHIAHWWSPDGTRLAYATINDSRVPLMELPTYTGSVYPTVKPYHY PKAGSENPSISLHVIGLNGPTHDLEMMPPDDPRMREYYITMVKWATSTKVAVTWLNRAQNVSILTLCDAT TGVCTKKHEDESEAWLHRQNEEPVFSKDGRKFFFVRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITSGD WDVTKILSYDEKRNKIYFLSTEDLPRRRHLYSANTVDDFNRQCLSCDLVENCTYVSASFSHNMDFFLLKC EGPGVPTVTVHNTTDKRRMFDLEANEEVQKAINDRQMPKIEYRKIEVEDYSLPMQILKPATFTDTAHYPL LLVVDGTPGSQSVTERFEVTWETVLVSSHGAVVVKCDGRGSGFQGTKLLQEVRRRLGFLEEKDQMEAVRT MLKEQYIDKTRVAVFGKDYGGYLSTYILPAKGENQGQTFTCGSALSPITDFKLYASAFSERYLGLHGLDN RAYEMTKLAHRVSALEDQQFLIIHATADEKIHFQHTAELITQLIKGKANYSLQIYPDESHYFHSVALKQH LSRSIIGFFVECFRVQDKLPTATAKEEEEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198886
ORF Size	2403 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198886.1, NP_001185815.1
RefSeq Size	4615 bp
RefSeq ORF	2406 bp
Locus ID	13483
MW	91.6 kDa
Gene Summary	Promotes cell surface expression of the potassium channel KCND2 (PubMed:22311982). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:22311982). Has no dipeptidyl aminopeptidase activity (By similarity).[UniProtKB/Swiss-Prot Function]

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