Palb2 (NM_001289843) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC066140
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231047 representing NM_001289843 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTATATTTACAGTAAACTCAGTAGTATATAAAGCTGTGCGTGCCCATGGTCAGCTGCCAGGAAGTC CCAATTCTTGTTCTGTAAATGATCTCACGCATAGTAACTTGCCAGCAAATAGTACCCCAAACTCTAAATC TTTAAAATCTCCCAGTAACACTGTTGATGAGAGAAATGAACCTCTTCAGGAAGATGAAATTCTTGGTCCA TCTAAGAACTTCAACCTGGCAGCAGTCTCTCCTCCTTCCACAGAAAGTCAAATACATTCTTGTACTATGC TTGAAGGCCTTCTGTTTCCTGCAGAATACTATGTTAGAACAACCCGTCGTATGTCAGATTGTCAGAGAAA AATAGCTCTGGAAGCTGTAATTCAAAGTCATTTGGGTGTCAAAAAGAAAGAGCTTAAAAAGAAAACCAAA GCTACTAAGGCGGTCGTCCTCTCCAGTGAAGACACTGACCAGAGTGAAAGTGGCATGCTGGACACGAGCA CGGGACAGTCCAGTTCAGGAAGCCTCTCTCAGAAACTGCTCTCACCAGCTGAGGTCAGCTCTCCTCCAGG ACCTGCTGGAAAGGCCACCACACCGCCACCTGGTAGAGGACACAGAGGGAAACGAAAATCAGCCCGCACC TCCACACTGGGTCACTGCCAGCTGCTTTTTCCTCCTTGTGCCGCACTGGCTGTTAACAGGTCCAAGGGCA AATTCACCAAGCATAAATGTCAGAACAGAGGCGTGGTTATTCATGACTTTGAGTTACCTGATGAAGACTT TGGGCTTCTTAAACTTGAAAAATTGAAGTCCTGCTCGGAAAAACTGATTGAGTCTCCTGACTCAAAAAAC TGTGGTGAGAGGCTTCCTAGAGAAGGAAACCATGCTGCTCTGGAGGAACTGCAAAGAGATTCGGAGACGG AGGGCTTGGAAGAGGAGCTCACTGTTCCACCAGGAGAAGCGTACCGTCCAGGGCCAACCCTGAGAAGGCA GCCAGGGAGCAAGGACCTTTCTTCATCCATAGTGCTTTTCACTCCTGCAGACACTGCTGCGCCTAACGAC AGTGGCAGGCCTCCTCCCTCCCTGTGCTCACCTGCTTTCCCCATCTTAGGCATGACTCCAGCTCTTGGCT CCCAAGCAGCCGGTGAGACTCTATCTACTGAAGCTGCACAGCCTTGCTCTACATCCCAACCTCCTCTCTT GGGAGACACAAACAGTCTTGTCAATAACAGTAAACAGTGCAACAGTTCAGCCTGCTCACCAAAACCGGAC ACCAACCTGCAAGCGTCAGGTAGGCAAGGACAACCTGCCTGTGACAGTGACTCTGGCCCCCAAGCAACAC CTCTACCTGTTGAGTCATTCACTTTCAGAGAAAATCAGCTTTGTGGAAATGCATGCCTCGAGTTACATGA ACATTCCACTGAACAGACTGAAACTGCAGATCGCCCTGCTTGTGACAATCTAAACCCAGGAAACCTACAG TTGGTTTCAGAGTTAAAGAATCCTTCCAGTTCCTGCTCTGTGGATGTGAGCGCCATGTGGTGGGAAAGAG CTGGTGCTAAGGAGCCATGTATCGTAACTGCTTGTGAAGATGTAGTTTCTCTTTGGAAACCCTTGAATTC TCTGCAGTGGGAGAAAGTTCATACCTGGCACTTCACAGAGGTTCCAGTATTACAAATAGTTCCAGTGCCT GATGTTTACAATCTTATATGTGTAGCTTTGGGAAGTTTGGAAATCAGAGAAATCAGGGCGTTGCTGTGCT CTTCTGGTGATGACAGTGAAAAGCAAGTCCTCCTGAAATCTGGAGATATAAAAGCTATGCTTGGCCTGAC AAAGCGGAGGCTAGTTAGTAGCACTGGGACCTTTTGCAATCAACAAATACAAATCATGACATTTGCTGAC GACGGAAGCAGCAAAGATGAACAGCTTTTGATGCCTCCTGATGAGACTGTACTGACTTTTGCTGAAGTCC AAGGGACGCAGGAAGCTCTGCTTGGTACTACCACTGTGAACAGCATTGTGATCTGGAATTTAAAAACCGG CCAGCTCCTGAAGAAGATGCACATTGATGACTCCTACCAGGCTTCGGTCTGTCACGGAGCCTATTCTGAG AAGGTTCCCTGTAGCCCAGGCTGGCCTGCACCTGTCTATGTCCTGAGTAAGATGACCTTGAACTTACGAT TCCCTGCTTCTACCTCTCCAGTGCTGGGCTTTCTGGCACAAGTCAGGCTGAGCTACAATCCTATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231047 representing NM_001289843 Red=Cloning site Green=Tags(s) MTIFTVNSVVYKAVRAHGQLPGSPNSCSVNDLTHSNLPANSTPNSKSLKSPSNTVDERNEPLQEDEILGP SKNFNLAAVSPPSTESQIHSCTMLEGLLFPAEYYVRTTRRMSDCQRKIALEAVIQSHLGVKKKELKKKTK ATKAVVLSSEDTDQSESGMLDTSTGQSSSGSLSQKLLSPAEVSSPPGPAGKATTPPPGRGHRGKRKSART STLGHCQLLFPPCAALAVNRSKGKFTKHKCQNRGVVIHDFELPDEDFGLLKLEKLKSCSEKLIESPDSKN CGERLPREGNHAALEELQRDSETEGLEEELTVPPGEAYRPGPTLRRQPGSKDLSSSIVLFTPADTAAPND SGRPPPSLCSPAFPILGMTPALGSQAAGETLSTEAAQPCSTSQPPLLGDTNSLVNNSKQCNSSACSPKPD TNLQASGRQGQPACDSDSGPQATPLPVESFTFRENQLCGNACLELHEHSTEQTETADRPACDNLNPGNLQ LVSELKNPSSSCSVDVSAMWWERAGAKEPCIVTACEDVVSLWKPLNSLQWEKVHTWHFTEVPVLQIVPVP DVYNLICVALGSLEIREIRALLCSSGDDSEKQVLLKSGDIKAMLGLTKRRLVSSTGTFCNQQIQIMTFAD DGSSKDEQLLMPPDETVLTFAEVQGTQEALLGTTTVNSIVIWNLKTGQLLKKMHIDDSYQASVCHGAYSE KVPCSPGWPAPVYVLSKMTLNLRFPASTSPVLGFLAQVRLSYNPID TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289843
ORF Size	2238 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289843.1, NP_001276772.1
RefSeq Size	3253 bp
RefSeq ORF	2241 bp
Locus ID	233826
UniProt ID	Q3U0P1
MW	80.9 kDa
Gene Summary	Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).[UniProtKB/Swiss-Prot Function]

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